Overexpression of Sperm Acrosomal SLLP1 Binding protein (SAS1B) in various cancer types, including uterine cancer cells, was discovered a few years ago, and different monoclonal antibodies (anti-SAS1B) that specifical...Overexpression of Sperm Acrosomal SLLP1 Binding protein (SAS1B) in various cancer types, including uterine cancer cells, was discovered a few years ago, and different monoclonal antibodies (anti-SAS1B) that specifically bind to SAS1B antigens were developed. Labeling of these antibodies with radionuclides can provide an opportunity for imaging and radioimmunotherapy. The objective of this study was to label anti-SAS1B (SB5) with Zirconium-89 (<sup>89</sup>Zr) for PET imaging and determine its biodistribution. Anti-SAS1B (SB5) antibody was labeled with <sup>89</sup>Zr indirectly using the chelator desferrioxamine B (DFO), which is currently a best linker for<sup> 89</sup>Zr. The antibody, SB5, was first conjugated to DFO with a ratio of 1:5 and then labeled with 250 μCi of <sup>89</sup>Zr. Results of PET imaging in mouse-bearing uterine cancer tumor showed a limited uptake. The bio-distribution study matched the PET imaging and confirmed the uptake by the tumor, and the accumulation in bones. In conclusion, labeling of anti-SAS1B could provide an effective way of uterine cancer detection and treatment progression.展开更多
Nanographene oxide (NGO) is currently being explored for various biomedical applications.However,little information is known about its biological behaviors in vitro and in vivo.For further studying its pharmacokinetic...Nanographene oxide (NGO) is currently being explored for various biomedical applications.However,little information is known about its biological behaviors in vitro and in vivo.For further studying its pharmacokinetics and related biological behaviors in living systems,an effective and convenient tracing method is particularly demanded.In this work,NGO was labeled with radionuclide 188Re (188Re-NGO).To obtain high labeling yield and purity,a number of labeling conditions,including concentration of SnCl2 and ascorbic acid,reaction time and temperature,and pH were optimized,and stability of the 188Re-NGO in vitro and in vivo was evaluated.The results showed that NGO could be effectively labeled with high yield.The purified 188Re-NGO showed high stability in vitro and in vivo.A pretest of NGO biodistribution with single photon emission computed tomography showed that the 188Re-NGO was rapidly taken by organs such as lungs,liver,and spleen.The biodistribution of 188Re-NGO differs significantly from the free radionuclide,indicating that the labeling procedure is highly suitable for investigating its biological behavior in living systems.展开更多
Chitosan/bovine serum albumin (BSA) ultrathin films were fabricated on modified quartz wafer through layer by layer deposition.The effects of experimental conditions, such as pH value, molecular weight of CS, ionic st...Chitosan/bovine serum albumin (BSA) ultrathin films were fabricated on modified quartz wafer through layer by layer deposition.The effects of experimental conditions, such as pH value, molecular weight of CS, ionic strength and polyanions coexisting in BSA solution, on the fabrication and the stability of films were investigated with a radio\|labeled method. The results show that the absorbed BSA is almost proportional to the number of assembly cycle except the first 2 cycles, and chitosan/BSA films are favorable to be fabricated in chitosan aq AcOH at pH=5.2 as compared with at pH=1.0. When two different molecular weight (MW) chitosans were used ( M η≈1.2×10 5, M η≈1.0×10 6), the lower MW chitosan adsorbs more BSA, but less stable ultrathin films are fabricated. Similar results were obtained that the amount of adsorbed BSA is higher but the films formed are less stable in water than in phosphate buffered saline (PBS). The adsorption of BSA is restricted greatly in the presence of poly(sodium styrene sulfonate) (PSS) mainly because of the competitive adsorption.展开更多
文摘Overexpression of Sperm Acrosomal SLLP1 Binding protein (SAS1B) in various cancer types, including uterine cancer cells, was discovered a few years ago, and different monoclonal antibodies (anti-SAS1B) that specifically bind to SAS1B antigens were developed. Labeling of these antibodies with radionuclides can provide an opportunity for imaging and radioimmunotherapy. The objective of this study was to label anti-SAS1B (SB5) with Zirconium-89 (<sup>89</sup>Zr) for PET imaging and determine its biodistribution. Anti-SAS1B (SB5) antibody was labeled with <sup>89</sup>Zr indirectly using the chelator desferrioxamine B (DFO), which is currently a best linker for<sup> 89</sup>Zr. The antibody, SB5, was first conjugated to DFO with a ratio of 1:5 and then labeled with 250 μCi of <sup>89</sup>Zr. Results of PET imaging in mouse-bearing uterine cancer tumor showed a limited uptake. The bio-distribution study matched the PET imaging and confirmed the uptake by the tumor, and the accumulation in bones. In conclusion, labeling of anti-SAS1B could provide an effective way of uterine cancer detection and treatment progression.
基金Supported by the National Science Foundation of China(No.10775169,10905086,10975179)the Shanghai Municipal Natural Science Foundation(No.08ZR1422700,08JC1422600)+2 种基金the Ministry of Health(No.2009ZX10004-301)the CAS Innovation Program(No.095501K)the MOST973Program(No.2006CB705605)
文摘Nanographene oxide (NGO) is currently being explored for various biomedical applications.However,little information is known about its biological behaviors in vitro and in vivo.For further studying its pharmacokinetics and related biological behaviors in living systems,an effective and convenient tracing method is particularly demanded.In this work,NGO was labeled with radionuclide 188Re (188Re-NGO).To obtain high labeling yield and purity,a number of labeling conditions,including concentration of SnCl2 and ascorbic acid,reaction time and temperature,and pH were optimized,and stability of the 188Re-NGO in vitro and in vivo was evaluated.The results showed that NGO could be effectively labeled with high yield.The purified 188Re-NGO showed high stability in vitro and in vivo.A pretest of NGO biodistribution with single photon emission computed tomography showed that the 188Re-NGO was rapidly taken by organs such as lungs,liver,and spleen.The biodistribution of 188Re-NGO differs significantly from the free radionuclide,indicating that the labeling procedure is highly suitable for investigating its biological behavior in living systems.
文摘Chitosan/bovine serum albumin (BSA) ultrathin films were fabricated on modified quartz wafer through layer by layer deposition.The effects of experimental conditions, such as pH value, molecular weight of CS, ionic strength and polyanions coexisting in BSA solution, on the fabrication and the stability of films were investigated with a radio\|labeled method. The results show that the absorbed BSA is almost proportional to the number of assembly cycle except the first 2 cycles, and chitosan/BSA films are favorable to be fabricated in chitosan aq AcOH at pH=5.2 as compared with at pH=1.0. When two different molecular weight (MW) chitosans were used ( M η≈1.2×10 5, M η≈1.0×10 6), the lower MW chitosan adsorbs more BSA, but less stable ultrathin films are fabricated. Similar results were obtained that the amount of adsorbed BSA is higher but the films formed are less stable in water than in phosphate buffered saline (PBS). The adsorption of BSA is restricted greatly in the presence of poly(sodium styrene sulfonate) (PSS) mainly because of the competitive adsorption.
