Objective To develop a sensitive,simple,and accurate method for the determination of shionone in rat plasma after ig administration of Asteris Radix petroleum ether extract(RAPE).Methods The separation was achieved by...Objective To develop a sensitive,simple,and accurate method for the determination of shionone in rat plasma after ig administration of Asteris Radix petroleum ether extract(RAPE).Methods The separation was achieved by HPLC on a RP18 column(150 mm × 3.9 mm,5 μm) with a mobile phase composed of acetonitrile-0.05% phosphoric acid water(98:2) at a flow rate of 1.0 mL/min.UV Detector was set at 200 nm and friedelin was chosen as an internal standard.Results The linear range of the standard curves was(0.3443-22.0) μg/mL with the correlation coefficient of 0.9968.The intra-and inter-day precisions were all below 10% and the relative error was -3.5%-1.1%.Conclusion The developed method can be successfully applied to the pharmacokinetic study.After ig administration of RAPE,T1/2(ka) is(33.09 ± 7.32) min and T1/2(ke) is(84.95 ± 22.34) min.展开更多
基金support from the scientific and technological project of Hebei Province,China (No 09276423D)
文摘Objective To develop a sensitive,simple,and accurate method for the determination of shionone in rat plasma after ig administration of Asteris Radix petroleum ether extract(RAPE).Methods The separation was achieved by HPLC on a RP18 column(150 mm × 3.9 mm,5 μm) with a mobile phase composed of acetonitrile-0.05% phosphoric acid water(98:2) at a flow rate of 1.0 mL/min.UV Detector was set at 200 nm and friedelin was chosen as an internal standard.Results The linear range of the standard curves was(0.3443-22.0) μg/mL with the correlation coefficient of 0.9968.The intra-and inter-day precisions were all below 10% and the relative error was -3.5%-1.1%.Conclusion The developed method can be successfully applied to the pharmacokinetic study.After ig administration of RAPE,T1/2(ka) is(33.09 ± 7.32) min and T1/2(ke) is(84.95 ± 22.34) min.
