Mechanism of"Radix clematidis-impatientis semen"in the treatment of esophageal cancer based on network pharmacology

Objective:To explore the mechanism of“Radix clematidis-Impatientis semen”in the treatment of esophageal cancer based on network pharmacology.Methods:The active components of Radix clematidis and Impatientis semen were searched and selected through the TCMSP database,and supplemented with the literature,the targets of active components were predicted by Swiss Target Prediction platform.The main targets of esophageal cancer were obtained by Genecards,TTD and DisGeNet databases,and the key targets of“Radix clematidis-Impatientis semen”for the treatment of esophageal cancer were obtained by using Venn diagram analysis.The"drug-active ingredient-disease-target"network of“Radix clematidis-Impatientis semen”in the treatment of esophageal cancer was constructed with the help of Cytoscape3.7.2,and the key target PPI network was constructed by using STRING platform and Cytoscape software to find the core target.Metascape platform was used for GO and KEGG enrichment analysis of key targets,and the network diagram of"active componenttarget-pathway"was drawn.Results:There were 17 active components such as quercetin,kaempferol,3-epioleanolic acid and oleanolic acid in“Radix clematidis-Impatientis semen”,corresponding to 379 drug targets.178targets were obtained by mapping with 2396 disease targets of esophageal cancer,including PIK3CA,PIK3R1,SRC,MAPK1,MAPK3 and so on.KEGG enrichment analysis mainly involved PI3K-Akt,Rap1,Ras,VEGF signaling pathways,etc.Conclusion:This study preliminarily discusses the potential mechanism of“Radix clematidis-Impatientis semen”in the treatment of esophageal cancer,which provides a basis and new thought for further experimental research.