To investigate the effects of Radix pseudostellariae fibrous root extracts(RPFRE) on serum cytokines in normal and immunosuppressed mice and the correlation between indexes, KM male mice were randomly divided into fou...To investigate the effects of Radix pseudostellariae fibrous root extracts(RPFRE) on serum cytokines in normal and immunosuppressed mice and the correlation between indexes, KM male mice were randomly divided into four groups, which were given 0(control), 0.1, 0.2, 0.4 g/kg RPFRE by gavage. The levels of IL-2, IL-4, IL-6, and IFN-γ in the serum of mice were measured 14 d after gavage. On the 15day, the remaining mice in the experimental group were intraperitoneally injected with cyclophosphamide(CY), and samples were collected on the 18day to measure serum cytokines levels. The results showed that RPFRE improved the serum cytokine level in normal mice. 0.1 g/kg RPFRE significantly increased the content of IL-2and IFN-γ(P<0.05), while 0.4 g/kg RPFRE significantly increased the content of IL-4 and IL-6(P<0.05). Correlation analysis showed that serum cytokines IL-4 and IL-6 in normal mice had an extremely significant positive correlation with RPFRE concentration(P<0.01), IL-2 had a significant or extremely significant positive correlation with IL-4, IL-6, and IFN-γ(P<0.01or P<0.05), and IL-4 had an extremely significant positive correlation with IL-6(P<0.01). After CY injection, serum cytokine levels in mice decreased and immune function was inhibited. Compared with the control group, the experimental group recovered the level of cytokines to some extent. 0.1 g/kg RPFRE significantly increased the content of IL-2 in immunosuppressed mice(P<0.05), and 0.4 g/kg RPFRE significantly increased the content of IL-4, IL-6, and IFN-γ in immunosuppressed mice(P<0.05). Correlation analysis showed that the serum cytokines in immunosuppressed mice were significantly positively correlated with RPFRE concentration(P<0.01), and there was a certain dose dependence. Moreover, the correlation coefficient between IL-2 and IL-4, IL-6, and IFN-γ, IL-4 and IL-6, and IL-6 and IFN-γ reached an extremely significant level(P<0.01). To sum up, RPFRE could improve the serum cytokines level of mice, promote the secretion of cytokines, and have a certain immune protective effect. There was a significant positive correlation between the RPFRE concentration and the serum cytokines in mice, which provided basic data support for the further study of RPFRE immune function.展开更多
[Objective] The paper was to explore the immunoprotection effects of Radix Pseudostellariae fibrous root extraction(RPFRE) on experi-mental mice and its relationship with antioxidant function. [Method] KM male mice we...[Objective] The paper was to explore the immunoprotection effects of Radix Pseudostellariae fibrous root extraction(RPFRE) on experi-mental mice and its relationship with antioxidant function. [Method] KM male mice were randomly divided into 4 groups based on RPFRE gradient concentration, and the immune function indexes and antioxidant indexes of mice were determined at 14 d post intragastric administration of RPFRE.The remaining mice in the experimental group were intraperitoneally injected with cyclophosphamide(CY) from the 15^(th) to 17^(th) day, and samples were collected to determine the above indexes on the 18^(th) day. [Result] Intragastric administration of RPFRE for 14 d improved the spleen index,thymus index, T/B cell stimulation index and total antioxidant capacity(T-AOC) of normal mice, and extremely increased the thymus index and TAOC(P<0.01). The T-AOC had extremely positive correlation with the spleen index, thymus index and B cell stimulation index( P<0.01), and had significantly positive correlation with the T cell stimulation index(P<0.05). From the 15^(th) to 17^(th) day of the experiment, the immune function indexes and antioxidant indexes of mice decreased and the malondialdehyde(MDA) content increased. The experimental groups of 0.1, 0.2 and 0.4 g/kg RPFRE promoted the immunoprotection and antioxidant effect of mice. RPFRE concentration had extremely positive correlation with 4 indexes of immune function and antioxidant indexes T-AOC and SOD(P<0.01), but had extremely negative correlation with MDA(P<0.01). There was a significantly positive correlation between immune function indexes and antioxidant indexes of mice after CY intervention( P<0.05 or P<0.01). [Conclusion] RPFRE can improve the immune function indexes and antioxidant indexes of normal mice and immunosuppressed mice and has immunopro-tection effect on immunosuppressed mice, and there is a significant correlation between immune function indexes and antioxidant indexes of mice.展开更多
文摘To investigate the effects of Radix pseudostellariae fibrous root extracts(RPFRE) on serum cytokines in normal and immunosuppressed mice and the correlation between indexes, KM male mice were randomly divided into four groups, which were given 0(control), 0.1, 0.2, 0.4 g/kg RPFRE by gavage. The levels of IL-2, IL-4, IL-6, and IFN-γ in the serum of mice were measured 14 d after gavage. On the 15day, the remaining mice in the experimental group were intraperitoneally injected with cyclophosphamide(CY), and samples were collected on the 18day to measure serum cytokines levels. The results showed that RPFRE improved the serum cytokine level in normal mice. 0.1 g/kg RPFRE significantly increased the content of IL-2and IFN-γ(P<0.05), while 0.4 g/kg RPFRE significantly increased the content of IL-4 and IL-6(P<0.05). Correlation analysis showed that serum cytokines IL-4 and IL-6 in normal mice had an extremely significant positive correlation with RPFRE concentration(P<0.01), IL-2 had a significant or extremely significant positive correlation with IL-4, IL-6, and IFN-γ(P<0.01or P<0.05), and IL-4 had an extremely significant positive correlation with IL-6(P<0.01). After CY injection, serum cytokine levels in mice decreased and immune function was inhibited. Compared with the control group, the experimental group recovered the level of cytokines to some extent. 0.1 g/kg RPFRE significantly increased the content of IL-2 in immunosuppressed mice(P<0.05), and 0.4 g/kg RPFRE significantly increased the content of IL-4, IL-6, and IFN-γ in immunosuppressed mice(P<0.05). Correlation analysis showed that the serum cytokines in immunosuppressed mice were significantly positively correlated with RPFRE concentration(P<0.01), and there was a certain dose dependence. Moreover, the correlation coefficient between IL-2 and IL-4, IL-6, and IFN-γ, IL-4 and IL-6, and IL-6 and IFN-γ reached an extremely significant level(P<0.01). To sum up, RPFRE could improve the serum cytokines level of mice, promote the secretion of cytokines, and have a certain immune protective effect. There was a significant positive correlation between the RPFRE concentration and the serum cytokines in mice, which provided basic data support for the further study of RPFRE immune function.
基金Supported by Special Project for the Central Government Guiding Local Scientific and Technological Development(2021L3030)Horizontal Project of School-enterprise Cooperation of Fujian Agriculture and Forestry University(104/KH180151A)。
文摘[Objective] The paper was to explore the immunoprotection effects of Radix Pseudostellariae fibrous root extraction(RPFRE) on experi-mental mice and its relationship with antioxidant function. [Method] KM male mice were randomly divided into 4 groups based on RPFRE gradient concentration, and the immune function indexes and antioxidant indexes of mice were determined at 14 d post intragastric administration of RPFRE.The remaining mice in the experimental group were intraperitoneally injected with cyclophosphamide(CY) from the 15^(th) to 17^(th) day, and samples were collected to determine the above indexes on the 18^(th) day. [Result] Intragastric administration of RPFRE for 14 d improved the spleen index,thymus index, T/B cell stimulation index and total antioxidant capacity(T-AOC) of normal mice, and extremely increased the thymus index and TAOC(P<0.01). The T-AOC had extremely positive correlation with the spleen index, thymus index and B cell stimulation index( P<0.01), and had significantly positive correlation with the T cell stimulation index(P<0.05). From the 15^(th) to 17^(th) day of the experiment, the immune function indexes and antioxidant indexes of mice decreased and the malondialdehyde(MDA) content increased. The experimental groups of 0.1, 0.2 and 0.4 g/kg RPFRE promoted the immunoprotection and antioxidant effect of mice. RPFRE concentration had extremely positive correlation with 4 indexes of immune function and antioxidant indexes T-AOC and SOD(P<0.01), but had extremely negative correlation with MDA(P<0.01). There was a significantly positive correlation between immune function indexes and antioxidant indexes of mice after CY intervention( P<0.05 or P<0.01). [Conclusion] RPFRE can improve the immune function indexes and antioxidant indexes of normal mice and immunosuppressed mice and has immunopro-tection effect on immunosuppressed mice, and there is a significant correlation between immune function indexes and antioxidant indexes of mice.
