RAPD analysis for genetic diversity of germplasm resources of <i>Strobilanthes</i>

The 18 samples representing 18 populations of Strobilanthes cusia (Nees) O. Ktze in Fujian Province of China were analyzed with RAPD markers. Eleven primers were used, a total of 106 bands were scored and 88 of them were polymorphic. The percentage of polymorphic loci was 77.36%, Nei’s gene diversity was 0.2420, and Shannon’s index was 0.3700. The 18 populations were classified into 2 groups based on the RAPD data by the method of hierarchical cluster analysis. Most of the populations from Fujian were clustered into a group, other populations were clustered into the other group. There was a high level of genetic diversity among the populations, and the genetic differentiation was obvious among the populations from Fujian.

马蓝的化学成分研究

马蓝Strobilanthes cusia(Nees)O.Ktze.系爵床科植物,主要分布于华南、西南各地。马蓝有泻火、解毒、凉血等功效,是大青叶、板蓝根药材及制剂的主要来源之一。常用于治疗流感、流行性腮腺炎、流行性乙型脑炎、肝炎等疾病。化学成分据报道有氨基酸、靛蓝、靛玉红、色胺酮、β-谷甾醇、羽扇豆醇、白桦脂醇、羽扇酮。在我们的研究中除得到羽扇豆醇(Ⅰ)、白桦脂醇(Ⅱ)。

大青叶、板蓝根和青黛的本草考证

根据现代应用和对本草记载的考证,大青叶、板蓝根和青黛的原植物有多种,它们是蓼蓝(Poly-gonum tinctorium Ait.)、马蓝(Stno-kbilantheo cusia(Nees)O.Ktze.)、大青(Clenodendrum cyrtophyllum Tu-rez.)和菘蓝(Isatis indigotica Fort,)等。用作配方时,以植物蓼蓝的叶作大青叶为宜,马蓝的根作板蓝根为宜,而制作青黛则宜选用菘蓝为原料。