Objective： To investigate the effect of Radix Isatidis polysaccharides （RIP） on the immunological function and expression of immune related cytokines in mice. Methods： Sixty mice were randomly divided into six gro...Objective： To investigate the effect of Radix Isatidis polysaccharides （RIP） on the immunological function and expression of immune related cytokines in mice. Methods： Sixty mice were randomly divided into six groups, the normal group, cyclophosphamide （Cy） model group, Levamisole （positive control） group, RIP low, medium and high dose groups （0.08 g/kg, 0.16 g/kg, 0.32 g/kg, respectively）, ten in each group. By detecting the value of abdominal macrophage phagocytic index, serum hemolysin （HC50）, proliferation of lymphocytes and expression of related cytokines, interleukin （IL-2） and interferon γ, （INF-γ）, the effect of RIP on immunological function and its mechanism were studied. Results： RIP could improve the level of hemolysin in immunological function depressed mice. The results showed that the value of macrophage phagocytic index in the high dose RIP group increased from 1.11 ±0.13 to 1.42±0.26. The level of IL-2 and INF- γ, could be decreased by Cy to 38.12±6.88 ng/L and 139.23±29.87 ng/L, respectively, while RIP in high dose could increase the secretion of IL-2 and INF- γ, to 53.54 ± 14.43 ng/L and 189.91 ± 32.63 ng/L, respectively. Conclusion： RIP could enhance non-specific immunological function, humoral immunity and cellular immunity in mice.展开更多
The Radix Isatidis polysaccharides (IIP) injected ip at the dose of 50mg/kg daily for seven days increased the spleen index. the total numbero of leukocytes and lymphocytes,the percentage of ANAE+ lymphocytes and the ...The Radix Isatidis polysaccharides (IIP) injected ip at the dose of 50mg/kg daily for seven days increased the spleen index. the total numbero of leukocytes and lymphocytes,the percentage of ANAE+ lymphocytes and the intensity of delayed type hyperoensitivity in normal mice as well as in immunosuppressed mice.Moreover,IIP could improve the phagocytic function of reticuloendothelial system,promote the yield of hemolysin,and increase the NK activity in norwal mice at the same dose.展开更多
基金Foundation of State Administration of Traditional Chinese Medicine(No.04-05ZP970)Military 11th Five-Year Foundation(No.06MA360)
文摘Objective： To investigate the effect of Radix Isatidis polysaccharides （RIP） on the immunological function and expression of immune related cytokines in mice. Methods： Sixty mice were randomly divided into six groups, the normal group, cyclophosphamide （Cy） model group, Levamisole （positive control） group, RIP low, medium and high dose groups （0.08 g/kg, 0.16 g/kg, 0.32 g/kg, respectively）, ten in each group. By detecting the value of abdominal macrophage phagocytic index, serum hemolysin （HC50）, proliferation of lymphocytes and expression of related cytokines, interleukin （IL-2） and interferon γ, （INF-γ）, the effect of RIP on immunological function and its mechanism were studied. Results： RIP could improve the level of hemolysin in immunological function depressed mice. The results showed that the value of macrophage phagocytic index in the high dose RIP group increased from 1.11 ±0.13 to 1.42±0.26. The level of IL-2 and INF- γ, could be decreased by Cy to 38.12±6.88 ng/L and 139.23±29.87 ng/L, respectively, while RIP in high dose could increase the secretion of IL-2 and INF- γ, to 53.54 ± 14.43 ng/L and 189.91 ± 32.63 ng/L, respectively. Conclusion： RIP could enhance non-specific immunological function, humoral immunity and cellular immunity in mice.
文摘The Radix Isatidis polysaccharides (IIP) injected ip at the dose of 50mg/kg daily for seven days increased the spleen index. the total numbero of leukocytes and lymphocytes,the percentage of ANAE+ lymphocytes and the intensity of delayed type hyperoensitivity in normal mice as well as in immunosuppressed mice.Moreover,IIP could improve the phagocytic function of reticuloendothelial system,promote the yield of hemolysin,and increase the NK activity in norwal mice at the same dose.
