Hydrogen sulfide(H2S)is considered as a protective factor against cardiovascular disorders.However,there are few reports on the effects of H2S in the central nervous system during stress or injury.Previous studies on ...Hydrogen sulfide(H2S)is considered as a protective factor against cardiovascular disorders.However,there are few reports on the effects of H2S in the central nervous system during stress or injury.Previous studies on goldfish have shown that astrocytic response occurs in the damaged and contralateral optic nerves.Glial fibrillary acidic protein(GFAP)concentration in the optic nerves of rainbow trout has not been measured previously.This study further characterized the astrocytic response in the optic nerve and the brain of a rainbow trout(Oncorhynchus mykiss)after unilateral eye injury and estimated the amount of H2S-producing enzyme cystathionineβ-synthase(CBS)in the brain of the rainbow trout.Within 1 week after unilateral eye injury,a protein band corresponding to a molecular weight of 50 kDa was identified in the ipsi-and contralateral optic nerves of the rainbow trout.The concentration of GFAP in the injured optic nerve increased compared to the protein concentration on the contralateral side.The results of a quantitative analysis of GFAP+cell distribution in the contralateral optic nerve showed the largest number of GFAP+cells and fibers in the optic nerve head.In the damaged optic nerve,patterns of GFAP+cell migration and large GFAP+bipolar activated astrocytes were detected at 1 week after unilateral eye injury.The study of H2S-producing system after unilateral eye injury in the rainbow trout was conducted using enzyme-linked immunosorbent assay,western blot analysis,and immunohistochemistry of polyclonal antibodies again st CBS in the integrative centers of the brain:telencephalon,optic tectum,and cerebellum.Enzyme-linked immunosorbent assay results showed a 1.7-fold increase in CBS expression in the rainbow trout brain at 1 week after unilateral eye injury compared with that in intact animals.In the ventricular and subventricular regions of the rainbow trout telencephalon,CBS+radial glia and neuroepithelial cells were identified.After unilateral eye injury,the number of CBS+neuroepithelial cells in the pallial and subpallial periventricular regions of the telencephalon increased.In the optic tectum,unilateral eye injury led to an increase in CBS expression in radial glial cells;simultaneously,the number of CBS+neuroepithelial cells decreased in intact animals.In the cerebellum of the rainbow trout,neuroglial interrelationships were revealed,where H2S was released,apparently,from astrocyte-like cells.The organization of H2S-producing cell complexes suggests that,the amount of glutamate produced in the rainbow trout cerebellum and its reuptake was controlled by astrocyte-like cells,reducing its excitotoxicity.In the dorsal matrix zone and granular eminences of the rainbow trout cerebellum,CBS was expressed in neuroepithelial cells.After unilateral eye injury,the level of CBS activity increased in all parts of the cerebellum.An increase in the number of H2S-producing cells was a response to oxidative stress after unilateral eye injury,and the overproduction of H2S in the cerebellum occurred to neutralize reactive oxygen species,providing the cells of the rainbow trout cerebellum with a protective effect.A structural reorganization in the dorsal matrix zone,associated with the appearance of an additional CBS+apical zone,and a decrease in the enzyme activity in the dorsal matrix zone,was revealed in the zones of constitutive neurogenesis.All experiments were approved by the Commission on Biomedical Ethics,A.V.Zhirmunsky National Scientific Center of Marine Biology(NSCMB),Far Eastern Branch,Russian Academy of Science(FEB RAS)(approval No.1)on July 31,2019.展开更多
In contrast to astrocytes in mammals, fish astrocytes promote axon regeneration after brain injury and actively participate in the regeneration process. Neurolin, a regeneration-associated, Zn8-1abeled protein, is inv...In contrast to astrocytes in mammals, fish astrocytes promote axon regeneration after brain injury and actively participate in the regeneration process. Neurolin, a regeneration-associated, Zn8-1abeled protein, is involved in the repair of damaged optic nerve in goldfish. At 1 week after unilateral eye injury, the ex- pression of neurolin in the optic nerve and chiasm, and the expression of Pax6 that influences nervous system development in various brain regions in the rainbow trout （Oncorhynchus mykiss） were detected. Immunohistochemical staining revealed that the number of Zn8~ cells in the optic nerve head and intraor- bital segment was obviously increased, and the increase in Zn8~ cells was also observed in the proximal and distal parts of injured optic nerve. This suggests that Zn8＊ astrocytes participate in optic nerve regeneration. ELISA results revealed that Pax6 protein increased obviously at 1 week post-injury. Immunohistochemi- cal staining revealed the appearance of Pax6＋ neurogenic niches and a larger number of neural precursor cells, which are mainly from Pax6＋ radial glia cells, in the nuclei of the diencephalon and optic tectum of rainbow trout （Oncorhynchus mykiss）. Taken together, unilateral eye injury can cause optic nerve reaction, and the formation of neurogenic niches is likely a compensation phenomenon during the repair process of optic nerve injury in rainbow trout （Oncorhynchus mykiss）.展开更多
The growth performance of a selected F1 rainbow trout genetically characterized as polymorphic with locus Omy207UoG (NA = 13, PIC = 0.891, HE = 0.9003) was evaluated in a recirculating aquaculture system (RAS). A set ...The growth performance of a selected F1 rainbow trout genetically characterized as polymorphic with locus Omy207UoG (NA = 13, PIC = 0.891, HE = 0.9003) was evaluated in a recirculating aquaculture system (RAS). A set of 157 rainbow trout of 37.8 g of mean body weight and 14.6 cm mean total length per tank, were introduced in each of six fish tank of 5 m3 connected to a recirculating aquaculture system (RAS). The trout was rearing during 317 days. Growth performance indicators and water quality were evaluated during the experiment. The genetically selected F1 rainbow trout showed a tendency towards homogeneity in growth performance along the culture period, reaching a mean total weight of 552.2 g. The length-weight relationship indicated an isometric growth (>3.0). This study presents the basis to establish a long-term marker-assisted selection program for rainbow trout culture in a subtropical region.展开更多
In order to screen the effective preventive drugs for saprolegniasis occurred during hatching of the fertilized eggs of golden trout, effects of two kinds of self-made Chinese herbal compounds（AB） and two kinds of c...In order to screen the effective preventive drugs for saprolegniasis occurred during hatching of the fertilized eggs of golden trout, effects of two kinds of self-made Chinese herbal compounds（AB） and two kinds of chemical bactericides（peroxyacetic acid andhydrogen peroxide, CD） with three disinfection concentrations were compared in this study. The fertilized eggs of golden trout were randomly divided into 13 groups, each group with two parallels of 1 000 eggs. Drugs at high,medium and low concentration were respectively used to immerse the fertilized eggs35 min for disinfection once a day. The numbers of live and dead eggs were recorded each day before disinfection and the numbers of survival eyed eggs were counted in each experimental group after finishing the whole test. The results indicated that all of four kinds of drugs used here had a certain effect on prevention and control of saprolegniasis during hatching of fertilized eggs of golden trout, and also showed a correlation between the concentrations and effects. After the selection of several drugs, we found that group B-medium, C-medium and D-high exhibited the most remarkable effects on control of saprolegniasis. However, careful consideration should be given to chemical drugs residues and their potential hazard to the eyed eggs. Therefore, Chinese herbal compound B was suggested to be used for prevention and control of saprolegniasis to achieve the healthy aquaculture. And the concentration and operation time of Chinese herbal compound B should be strictly controlled when used in disinfection of fertilized eggs.展开更多
In this study, the changes of the fatty acid composition of phospholipid in different tissues(muscle, heart, brain and spleen) of steelhead trout(Oncorhynchus mykiss) were analyzed when the water temperature decreased...In this study, the changes of the fatty acid composition of phospholipid in different tissues(muscle, heart, brain and spleen) of steelhead trout(Oncorhynchus mykiss) were analyzed when the water temperature decreased gradually from 16 to 12℃℃, 8℃, 6℃, 4℃, 2 and 1℃℃. Three fish individuals each tank(average weight 70.32 g ± 9.12 g) were collected and used to analysis at each designed temperatures. At normal temperature(16℃), the fatty acid composition of phospholipid of muscle and heart was similar each other. The highest concentration of saturate fatty acids(SFA) was found in the phospholipid of spleen. The brain phospholipid contained higher oleic acid(18:1 n9) than the phospholipid of other tissues at 16℃. When the environmental temperature decreased, the concentration of unsaturated fatty acids of phospholipids in all tissues increased, and accordingly the ratio pf the unsaturated to saturated fatty acids(U/S) and unsaturation index(UI) increased, indicating that steelhead trout can compensate temperature-dependent changes in membrane fluidity by remodeling the fatty acid composition of phospholipids. The changes in the fatty acid composition of phospholipid were tissue-specific. At the early stages of the experiment(16 to 8℃℃), the fatty acid composition of phospholipid changed remarkably in muscle, heart, and spleen. When temperature decreased to less than 8℃, an obvious response of phospholipid fatty acid was observed in all tissues. The change of phospholipid composition of steelhead trout tissues may be affected by both cold stress and starvation when the temperature decreased to 2℃, and the change of phospholipid composition of muscle was very obvious.展开更多
[ Objective] The study aimed to discuss the effects of ferrous fumarate on growth performance, blood biochemical parameters and content of trace element of Oncorhynchus mykiss. [ Method ] Juvenile rainbow trout with a...[ Objective] The study aimed to discuss the effects of ferrous fumarate on growth performance, blood biochemical parameters and content of trace element of Oncorhynchus mykiss. [ Method ] Juvenile rainbow trout with an initial weight of （89.2 ±0.2） g were fed with the basal fodder supplemented with different levels of ferrous fumarate （0, 20, 40, 80,160 and 480 mg/kg iron） for 60 d, and the six groups were named DO, D20, D40, D80, D160 and D480, wherein DO was as the control group, actually containing 62.60, 79.50, 99.60, 139.30, 215.20 and 538.40 mg/kg iron respectively. [ Result] The growth performance of juvenile rainbow trout was not affected by different dietary iron levels obviously （ P 〉 0.05）. With the increase of dietary iron level, hemoglobin （Hb） content and number of red blood cells（RBC） rose firstly and then leveled off. No significance was found in hematocrit （Hct） among the six groups （P 〉0.05）. Iron content in the whole body, vertebrae and muscle increased significantly with the improvement of dietary iron level（ P 〈0.05）, and iron concentration in the liver increased firstly and then leveled off in groups from D40 to D480. No significant difference in zinc content of the whole body was found among the six groups （ P〉0.05）, while zinc content in the vertebrae and muscle in control group was significantly higher than that of other groups （ P〈0.05）, and zinc concentration in the liver in groups DO and 1320 was significantly higher than that of other groups （ P 〈0.05）. Copper content in the whole body increased significantly with the increase of dietary iron level （P 〈 0.05 ）, while no significant difference was observed in the vertebrae （ P 〉0.05）, and copper concentration in the muscle in control group was significantly lower than that of other groups （ P 〈0.05）. Serum lysozyme （LZM） activity of group DO was significantly lower than that of other groups （ P 〈 0.05） except for group D480 （ P 〉 0.05）. Catalase （CAT） activity of serum enhanced significantly （ P 〈 0.05） from DO to EH0 and then decreased obviously （P 〈0.05）. [ Conclusion] Based on hemoglobin and iron content in the liver, the broken-line model analysis showed that the dietary iron level provided by ferrous fumarate for juvenile rainbow trout was estimated to be 99.8 and 100.4 mg/kg respectively in the experiment.展开更多
The inclusion of PepSoyGen (PSG), a commercially-available fermented soybean meal product, was evaluated with juvenile rainbow trout, Oncorhynchus mykiss in an initial 70-day feeding trial, with a supplemental trial i...The inclusion of PepSoyGen (PSG), a commercially-available fermented soybean meal product, was evaluated with juvenile rainbow trout, Oncorhynchus mykiss in an initial 70-day feeding trial, with a supplemental trial involving a subset of the experimental diets continuing for an additional 40 d. Six diets containing 0%, 10%, 20%, 30%, 40%, or 50% PSG, with the PSG directly replacing fish meal, were used in the first trial. There were no significant differences in weight gain or feed conversion ratio between the fish meal-based control diet and diets containing up to 30% PSG. However, weight gain was significantly reduced and feed conversion ratio significantly increased with the 40% and 50% PSG diets. No health assessment differences were observed in fish receiving any of the diets, and no evidence of gross gut inflammation was evident. There were no significant differences in weight gain or feed conversion ratio among the four dietary treatments ranging from 0% to 30% PSG which were fed for an additional 40 d after the initial 70-d trial (110 days total). Based on these results, juvenile rainbow trout diets can contain up to 30% PSG without any loss of rearing performance, thereby replacing at least 60% of the fish meal.展开更多
The implantation of acoustic transmitters into the peritoneal cavity of fish is typically performed by experienced surgeons. This study compared the effectiveness of an experienced and inexperienced surgeon performing...The implantation of acoustic transmitters into the peritoneal cavity of fish is typically performed by experienced surgeons. This study compared the effectiveness of an experienced and inexperienced surgeon performing two different types of transmitter insertion techniques on rainbow trout (<i>Oncorhynchus</i> <i>mykiss</i>). The methods used were either a traditional sutured ventral incision or a novel unsutured lateral incision. There was no significant difference in surgical duration, tag retention, or fish survival between the experienced and na<span style="font-family:Verdana, Helvetica, Arial;white-space:normal;background-color:#FFFFFF;">ï</span>ve surgeon. The time to complete surgical wound closure was not significantly different between the fish operated on by an experienced or na<span style="font-family:Verdana, Helvetica, Arial;white-space:normal;background-color:#FFFFFF;">ï</span>ve surgeon, and surgeon experience had no significant effect on the duration of inflammation. However, there were significant differences between the two surgical techniques, regardless of surgeon experience. The ventral incision with sutures method took approximately three times longer to complete than the lateral incision-only method. However, complete wound closure was significantly faster in the sutured ventral incision treatment compared to the unsutured lateral treatment. Post-surgery inflammation was over seven times longer in the fish with sutures compared to those only receiving a lateral incision. Tag retention was not significantly different between the two surgical methods. The results of this study indicate that the unsutured lateral surgical technique used in this experiment can be completed with minimal training and surgical experience in contrast to the standard technique requiring su<span>tures. These results also provide additional support to the use of sutureless</span> surgical techniques for acoustic transmitter implantation.展开更多
Distillers dried grain with solubles (DDGS) was evaluated in juvenile Shasta-strain rainbow trout Oncorhynchus mykiss diets during a 36-day feeding trial. Two experimental diets containing either 10% or 20% DDGS with ...Distillers dried grain with solubles (DDGS) was evaluated in juvenile Shasta-strain rainbow trout Oncorhynchus mykiss diets during a 36-day feeding trial. Two experimental diets containing either 10% or 20% DDGS with supplemented amino acids (lysine, methionine, isoleucine, and his-tidine) and phytase were compared to a fish meal-only control diet. Tanks of trout receiving diets containing either concentration of DDGS weighed significantly less at the end of the trial and had significantly poorer feed conversion ratios than tanks of fish being fed the fish meal- only control. There was no significant difference in individual fish length, weight, condition factor, or any fish health measurements among diet treatments. Both the hepatosomatic index and viscerosomatic index were significantly less in the fish fed 10% DDGS than those fed the control diet. Body fat was significantly greater in the fish receiving 20% DDGS compared to fish fed either of the other two diets. Fillet composition, as determined by crude protein, crude lipid, ash, and water, was not significantly different among fish reared on any of the diets. There was also no significant difference in estimated protein digestibility coefficients among fish receiving any of the diets. The results suggest that DDGS, even if supplemented with essential amino acids and phytase, will lead to decreased juvenile rainbow trout growth at dietary concentrations of at 10% or greater.展开更多
A novel sutureless surgical technique has been successfully used to implant acoustic transmitters in relatively large rainbow trout Oncorhynchus mykiss with a tag-to-body-ratio of 0.88%. This study examined the same t...A novel sutureless surgical technique has been successfully used to implant acoustic transmitters in relatively large rainbow trout Oncorhynchus mykiss with a tag-to-body-ratio of 0.88%. This study examined the same technique in smaller rainbow trout in two, 12-week trials comparing both sutured and sutureless surgical techniques. In the first trial using a tag-to-body-ratio of 1.9% ± 0.04%, tag retention was only 16.6% in fish without sutures, which was significantly lower than the 83.3% retention with sutured incisions. Similarly, in the second trial with a tag-to-body-ratio of 3.2% ± 0.03%, tag retention was 55.5% without sutures, which was significantly lower than the 90.0% retention using sutures. Mortality was not significantly different between treatments in either trial. The results of this study indicate that sutures must be used during surgeries to implant acoustic transmitters in relatively smaller fish with tag-to-body-ratios at or above 1.9%.展开更多
Hatchery-reared fish are frequently adjusted (tempered) to the higher water temperatures present in the water bodies where they are to be stocked. This study was undertaken to determine the necessity of such tempering...Hatchery-reared fish are frequently adjusted (tempered) to the higher water temperatures present in the water bodies where they are to be stocked. This study was undertaken to determine the necessity of such tempering practices. <span><span style="font-family:Verdana;">This study used rainbow trout (</span><i><span style="font-family:Verdana;">Oncorhynchus mykiss</span></i><span style="font-family:Verdana;">) and brown trout</span></span><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">Salmo trutta</span></i><span style="font-family:Verdana;">) reared at 11.2</span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">°C. </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">The first two trials for each species were designed to simultaneously determine the upper incipient lethal temperature and 100% lethal temperature over a 14-day period. The third trial for each species evaluated the effects of an exaggerated 12-hour tempering regime on fish survival after placement in elevated water temperatures. After transfer from a water temperature of 11.2°C, no rainbow trout survived at 26°C, and only 50% survived at 25°C. No brown trout survived at 22°C and only 50% at 20°C. Survival of rainbow trout was not improved by the 12-hour tempering regime where water temperatures were slowly increased from 11.2°C to either 25°C or 26°C. Similarly, tempering did not improve brown trout survival at either 20°C or 22°C. These results suggest that tempering is not needed when hatchery-reared trout are reared and stocked at the water temperatures within the range of those used in this study.</span></span></span>展开更多
基金supported by a grant from the President of the Russian Federation(No.MD-4318.2015.4 to EVP)a grant from the Program for Basic Research of the Far East Branch of the Russian Academy of Sciences 2015–2017(No.15-I-6-116,section III to EVP,AAV and DKO)
文摘Hydrogen sulfide(H2S)is considered as a protective factor against cardiovascular disorders.However,there are few reports on the effects of H2S in the central nervous system during stress or injury.Previous studies on goldfish have shown that astrocytic response occurs in the damaged and contralateral optic nerves.Glial fibrillary acidic protein(GFAP)concentration in the optic nerves of rainbow trout has not been measured previously.This study further characterized the astrocytic response in the optic nerve and the brain of a rainbow trout(Oncorhynchus mykiss)after unilateral eye injury and estimated the amount of H2S-producing enzyme cystathionineβ-synthase(CBS)in the brain of the rainbow trout.Within 1 week after unilateral eye injury,a protein band corresponding to a molecular weight of 50 kDa was identified in the ipsi-and contralateral optic nerves of the rainbow trout.The concentration of GFAP in the injured optic nerve increased compared to the protein concentration on the contralateral side.The results of a quantitative analysis of GFAP+cell distribution in the contralateral optic nerve showed the largest number of GFAP+cells and fibers in the optic nerve head.In the damaged optic nerve,patterns of GFAP+cell migration and large GFAP+bipolar activated astrocytes were detected at 1 week after unilateral eye injury.The study of H2S-producing system after unilateral eye injury in the rainbow trout was conducted using enzyme-linked immunosorbent assay,western blot analysis,and immunohistochemistry of polyclonal antibodies again st CBS in the integrative centers of the brain:telencephalon,optic tectum,and cerebellum.Enzyme-linked immunosorbent assay results showed a 1.7-fold increase in CBS expression in the rainbow trout brain at 1 week after unilateral eye injury compared with that in intact animals.In the ventricular and subventricular regions of the rainbow trout telencephalon,CBS+radial glia and neuroepithelial cells were identified.After unilateral eye injury,the number of CBS+neuroepithelial cells in the pallial and subpallial periventricular regions of the telencephalon increased.In the optic tectum,unilateral eye injury led to an increase in CBS expression in radial glial cells;simultaneously,the number of CBS+neuroepithelial cells decreased in intact animals.In the cerebellum of the rainbow trout,neuroglial interrelationships were revealed,where H2S was released,apparently,from astrocyte-like cells.The organization of H2S-producing cell complexes suggests that,the amount of glutamate produced in the rainbow trout cerebellum and its reuptake was controlled by astrocyte-like cells,reducing its excitotoxicity.In the dorsal matrix zone and granular eminences of the rainbow trout cerebellum,CBS was expressed in neuroepithelial cells.After unilateral eye injury,the level of CBS activity increased in all parts of the cerebellum.An increase in the number of H2S-producing cells was a response to oxidative stress after unilateral eye injury,and the overproduction of H2S in the cerebellum occurred to neutralize reactive oxygen species,providing the cells of the rainbow trout cerebellum with a protective effect.A structural reorganization in the dorsal matrix zone,associated with the appearance of an additional CBS+apical zone,and a decrease in the enzyme activity in the dorsal matrix zone,was revealed in the zones of constitutive neurogenesis.All experiments were approved by the Commission on Biomedical Ethics,A.V.Zhirmunsky National Scientific Center of Marine Biology(NSCMB),Far Eastern Branch,Russian Academy of Science(FEB RAS)(approval No.1)on July 31,2019.
基金supported by the President of the Russian Federation(grant No.MD-4318.2015.4 to EVP)+2 种基金by the Far East Branch of the Russian Academy of Sciences within the Program for Basic Research for 2015–2017(grant No.15-I-6-116 section Ⅲ to AAV and EVP)
文摘In contrast to astrocytes in mammals, fish astrocytes promote axon regeneration after brain injury and actively participate in the regeneration process. Neurolin, a regeneration-associated, Zn8-1abeled protein, is involved in the repair of damaged optic nerve in goldfish. At 1 week after unilateral eye injury, the ex- pression of neurolin in the optic nerve and chiasm, and the expression of Pax6 that influences nervous system development in various brain regions in the rainbow trout （Oncorhynchus mykiss） were detected. Immunohistochemical staining revealed that the number of Zn8~ cells in the optic nerve head and intraor- bital segment was obviously increased, and the increase in Zn8~ cells was also observed in the proximal and distal parts of injured optic nerve. This suggests that Zn8＊ astrocytes participate in optic nerve regeneration. ELISA results revealed that Pax6 protein increased obviously at 1 week post-injury. Immunohistochemi- cal staining revealed the appearance of Pax6＋ neurogenic niches and a larger number of neural precursor cells, which are mainly from Pax6＋ radial glia cells, in the nuclei of the diencephalon and optic tectum of rainbow trout （Oncorhynchus mykiss）. Taken together, unilateral eye injury can cause optic nerve reaction, and the formation of neurogenic niches is likely a compensation phenomenon during the repair process of optic nerve injury in rainbow trout （Oncorhynchus mykiss）.
基金funded by SAGARPA-CONACYT-2006-01-12147 to IDLABSCBS-UAMI-2007-2010-147.07.03 to IDLABS and JLAF
文摘The growth performance of a selected F1 rainbow trout genetically characterized as polymorphic with locus Omy207UoG (NA = 13, PIC = 0.891, HE = 0.9003) was evaluated in a recirculating aquaculture system (RAS). A set of 157 rainbow trout of 37.8 g of mean body weight and 14.6 cm mean total length per tank, were introduced in each of six fish tank of 5 m3 connected to a recirculating aquaculture system (RAS). The trout was rearing during 317 days. Growth performance indicators and water quality were evaluated during the experiment. The genetically selected F1 rainbow trout showed a tendency towards homogeneity in growth performance along the culture period, reaching a mean total weight of 552.2 g. The length-weight relationship indicated an isometric growth (>3.0). This study presents the basis to establish a long-term marker-assisted selection program for rainbow trout culture in a subtropical region.
基金Supported by the Twelfth Five-year Plan"Integration and Demonstration of Key Technology of Raising Fish in Heilongjiang"(2012BAD25B10)~~
文摘In order to screen the effective preventive drugs for saprolegniasis occurred during hatching of the fertilized eggs of golden trout, effects of two kinds of self-made Chinese herbal compounds（AB） and two kinds of chemical bactericides（peroxyacetic acid andhydrogen peroxide, CD） with three disinfection concentrations were compared in this study. The fertilized eggs of golden trout were randomly divided into 13 groups, each group with two parallels of 1 000 eggs. Drugs at high,medium and low concentration were respectively used to immerse the fertilized eggs35 min for disinfection once a day. The numbers of live and dead eggs were recorded each day before disinfection and the numbers of survival eyed eggs were counted in each experimental group after finishing the whole test. The results indicated that all of four kinds of drugs used here had a certain effect on prevention and control of saprolegniasis during hatching of fertilized eggs of golden trout, and also showed a correlation between the concentrations and effects. After the selection of several drugs, we found that group B-medium, C-medium and D-high exhibited the most remarkable effects on control of saprolegniasis. However, careful consideration should be given to chemical drugs residues and their potential hazard to the eyed eggs. Therefore, Chinese herbal compound B was suggested to be used for prevention and control of saprolegniasis to achieve the healthy aquaculture. And the concentration and operation time of Chinese herbal compound B should be strictly controlled when used in disinfection of fertilized eggs.
基金jointly funded by the National Natural Science Foundation of China (Nos. 31572634 and 31702364)the Fundamental Research Funds for the Central Universities of China (No. 20161205)+1 种基金the Key Research and Development Program of Shandong Province (Nos. 2016CYJS04A01 and 2017CXGC0106)Science and Technology Planning Project of Guangdong Province, China (No. 2017B030314052)
文摘In this study, the changes of the fatty acid composition of phospholipid in different tissues(muscle, heart, brain and spleen) of steelhead trout(Oncorhynchus mykiss) were analyzed when the water temperature decreased gradually from 16 to 12℃℃, 8℃, 6℃, 4℃, 2 and 1℃℃. Three fish individuals each tank(average weight 70.32 g ± 9.12 g) were collected and used to analysis at each designed temperatures. At normal temperature(16℃), the fatty acid composition of phospholipid of muscle and heart was similar each other. The highest concentration of saturate fatty acids(SFA) was found in the phospholipid of spleen. The brain phospholipid contained higher oleic acid(18:1 n9) than the phospholipid of other tissues at 16℃. When the environmental temperature decreased, the concentration of unsaturated fatty acids of phospholipids in all tissues increased, and accordingly the ratio pf the unsaturated to saturated fatty acids(U/S) and unsaturation index(UI) increased, indicating that steelhead trout can compensate temperature-dependent changes in membrane fluidity by remodeling the fatty acid composition of phospholipids. The changes in the fatty acid composition of phospholipid were tissue-specific. At the early stages of the experiment(16 to 8℃℃), the fatty acid composition of phospholipid changed remarkably in muscle, heart, and spleen. When temperature decreased to less than 8℃, an obvious response of phospholipid fatty acid was observed in all tissues. The change of phospholipid composition of steelhead trout tissues may be affected by both cold stress and starvation when the temperature decreased to 2℃, and the change of phospholipid composition of muscle was very obvious.
基金funded by Technical System of Modern Agricultural Industry (nycytx-50 )Agriculture Transfer Fund of Ministry of Science and Technology (03EFN213700155)+1 种基金Scientific and Technological Development Plan Project of Shandong Province (2010-2013)Taishan Scholar Post Fund about Aquatic Animal Nutrient and Feed of Shandong Province(HY201004)
文摘[ Objective] The study aimed to discuss the effects of ferrous fumarate on growth performance, blood biochemical parameters and content of trace element of Oncorhynchus mykiss. [ Method ] Juvenile rainbow trout with an initial weight of （89.2 ±0.2） g were fed with the basal fodder supplemented with different levels of ferrous fumarate （0, 20, 40, 80,160 and 480 mg/kg iron） for 60 d, and the six groups were named DO, D20, D40, D80, D160 and D480, wherein DO was as the control group, actually containing 62.60, 79.50, 99.60, 139.30, 215.20 and 538.40 mg/kg iron respectively. [ Result] The growth performance of juvenile rainbow trout was not affected by different dietary iron levels obviously （ P 〉 0.05）. With the increase of dietary iron level, hemoglobin （Hb） content and number of red blood cells（RBC） rose firstly and then leveled off. No significance was found in hematocrit （Hct） among the six groups （P 〉0.05）. Iron content in the whole body, vertebrae and muscle increased significantly with the improvement of dietary iron level（ P 〈0.05）, and iron concentration in the liver increased firstly and then leveled off in groups from D40 to D480. No significant difference in zinc content of the whole body was found among the six groups （ P〉0.05）, while zinc content in the vertebrae and muscle in control group was significantly higher than that of other groups （ P〈0.05）, and zinc concentration in the liver in groups DO and 1320 was significantly higher than that of other groups （ P 〈0.05）. Copper content in the whole body increased significantly with the increase of dietary iron level （P 〈 0.05 ）, while no significant difference was observed in the vertebrae （ P 〉0.05）, and copper concentration in the muscle in control group was significantly lower than that of other groups （ P 〈0.05）. Serum lysozyme （LZM） activity of group DO was significantly lower than that of other groups （ P 〈 0.05） except for group D480 （ P 〉 0.05）. Catalase （CAT） activity of serum enhanced significantly （ P 〈 0.05） from DO to EH0 and then decreased obviously （P 〈0.05）. [ Conclusion] Based on hemoglobin and iron content in the liver, the broken-line model analysis showed that the dietary iron level provided by ferrous fumarate for juvenile rainbow trout was estimated to be 99.8 and 100.4 mg/kg respectively in the experiment.
文摘The inclusion of PepSoyGen (PSG), a commercially-available fermented soybean meal product, was evaluated with juvenile rainbow trout, Oncorhynchus mykiss in an initial 70-day feeding trial, with a supplemental trial involving a subset of the experimental diets continuing for an additional 40 d. Six diets containing 0%, 10%, 20%, 30%, 40%, or 50% PSG, with the PSG directly replacing fish meal, were used in the first trial. There were no significant differences in weight gain or feed conversion ratio between the fish meal-based control diet and diets containing up to 30% PSG. However, weight gain was significantly reduced and feed conversion ratio significantly increased with the 40% and 50% PSG diets. No health assessment differences were observed in fish receiving any of the diets, and no evidence of gross gut inflammation was evident. There were no significant differences in weight gain or feed conversion ratio among the four dietary treatments ranging from 0% to 30% PSG which were fed for an additional 40 d after the initial 70-d trial (110 days total). Based on these results, juvenile rainbow trout diets can contain up to 30% PSG without any loss of rearing performance, thereby replacing at least 60% of the fish meal.
文摘The implantation of acoustic transmitters into the peritoneal cavity of fish is typically performed by experienced surgeons. This study compared the effectiveness of an experienced and inexperienced surgeon performing two different types of transmitter insertion techniques on rainbow trout (<i>Oncorhynchus</i> <i>mykiss</i>). The methods used were either a traditional sutured ventral incision or a novel unsutured lateral incision. There was no significant difference in surgical duration, tag retention, or fish survival between the experienced and na<span style="font-family:Verdana, Helvetica, Arial;white-space:normal;background-color:#FFFFFF;">ï</span>ve surgeon. The time to complete surgical wound closure was not significantly different between the fish operated on by an experienced or na<span style="font-family:Verdana, Helvetica, Arial;white-space:normal;background-color:#FFFFFF;">ï</span>ve surgeon, and surgeon experience had no significant effect on the duration of inflammation. However, there were significant differences between the two surgical techniques, regardless of surgeon experience. The ventral incision with sutures method took approximately three times longer to complete than the lateral incision-only method. However, complete wound closure was significantly faster in the sutured ventral incision treatment compared to the unsutured lateral treatment. Post-surgery inflammation was over seven times longer in the fish with sutures compared to those only receiving a lateral incision. Tag retention was not significantly different between the two surgical methods. The results of this study indicate that the unsutured lateral surgical technique used in this experiment can be completed with minimal training and surgical experience in contrast to the standard technique requiring su<span>tures. These results also provide additional support to the use of sutureless</span> surgical techniques for acoustic transmitter implantation.
文摘Distillers dried grain with solubles (DDGS) was evaluated in juvenile Shasta-strain rainbow trout Oncorhynchus mykiss diets during a 36-day feeding trial. Two experimental diets containing either 10% or 20% DDGS with supplemented amino acids (lysine, methionine, isoleucine, and his-tidine) and phytase were compared to a fish meal-only control diet. Tanks of trout receiving diets containing either concentration of DDGS weighed significantly less at the end of the trial and had significantly poorer feed conversion ratios than tanks of fish being fed the fish meal- only control. There was no significant difference in individual fish length, weight, condition factor, or any fish health measurements among diet treatments. Both the hepatosomatic index and viscerosomatic index were significantly less in the fish fed 10% DDGS than those fed the control diet. Body fat was significantly greater in the fish receiving 20% DDGS compared to fish fed either of the other two diets. Fillet composition, as determined by crude protein, crude lipid, ash, and water, was not significantly different among fish reared on any of the diets. There was also no significant difference in estimated protein digestibility coefficients among fish receiving any of the diets. The results suggest that DDGS, even if supplemented with essential amino acids and phytase, will lead to decreased juvenile rainbow trout growth at dietary concentrations of at 10% or greater.
文摘A novel sutureless surgical technique has been successfully used to implant acoustic transmitters in relatively large rainbow trout Oncorhynchus mykiss with a tag-to-body-ratio of 0.88%. This study examined the same technique in smaller rainbow trout in two, 12-week trials comparing both sutured and sutureless surgical techniques. In the first trial using a tag-to-body-ratio of 1.9% ± 0.04%, tag retention was only 16.6% in fish without sutures, which was significantly lower than the 83.3% retention with sutured incisions. Similarly, in the second trial with a tag-to-body-ratio of 3.2% ± 0.03%, tag retention was 55.5% without sutures, which was significantly lower than the 90.0% retention using sutures. Mortality was not significantly different between treatments in either trial. The results of this study indicate that sutures must be used during surgeries to implant acoustic transmitters in relatively smaller fish with tag-to-body-ratios at or above 1.9%.
文摘Hatchery-reared fish are frequently adjusted (tempered) to the higher water temperatures present in the water bodies where they are to be stocked. This study was undertaken to determine the necessity of such tempering practices. <span><span style="font-family:Verdana;">This study used rainbow trout (</span><i><span style="font-family:Verdana;">Oncorhynchus mykiss</span></i><span style="font-family:Verdana;">) and brown trout</span></span><span style="font-family:Verdana;"> (</span><i><span style="font-family:Verdana;">Salmo trutta</span></i><span style="font-family:Verdana;">) reared at 11.2</span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">°C. </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">The first two trials for each species were designed to simultaneously determine the upper incipient lethal temperature and 100% lethal temperature over a 14-day period. The third trial for each species evaluated the effects of an exaggerated 12-hour tempering regime on fish survival after placement in elevated water temperatures. After transfer from a water temperature of 11.2°C, no rainbow trout survived at 26°C, and only 50% survived at 25°C. No brown trout survived at 22°C and only 50% at 20°C. Survival of rainbow trout was not improved by the 12-hour tempering regime where water temperatures were slowly increased from 11.2°C to either 25°C or 26°C. Similarly, tempering did not improve brown trout survival at either 20°C or 22°C. These results suggest that tempering is not needed when hatchery-reared trout are reared and stocked at the water temperatures within the range of those used in this study.</span></span></span>
