BACKGROUND Gut microbiota dysbiosis is reportedly actively involved in autoimmune diseases such as type 1 diabetes mellitus(T1DM).However,the alterations in the gut microbiota and their correlation with fasting blood ...BACKGROUND Gut microbiota dysbiosis is reportedly actively involved in autoimmune diseases such as type 1 diabetes mellitus(T1DM).However,the alterations in the gut microbiota and their correlation with fasting blood glucose(FBG)in Chinese children with T1DM remain unclear.AIM To investigate alterations in the gut microbiota in Chinese children with T1DM and their associations with clinical indicators.METHODS Samples from 51 children with T1DM and 47 age-matched and gender-matched healthy controls were obtained,to explore the structural and functional alterations in the fecal microbiota.The V3-V4 regions of the 16S rRNA gene were sequenced on a MiSeq instrument,and the association with FBG were analyzed.RESULTS We found that the bacterial diversity was significantly increased in the T1DMassociated fecal microbiota,and changes in the microbial composition were observed at different taxonomic levels.The T1DM-reduced differential taxa,such as Bacteroides vulgatus ATCC8482,Bacteroides ovatus,Bacteroides xylanisolvens,and Flavonifractor plautii,were negatively correlated with FBG,while the T1DMenriched taxa,such as Blautia,Eubacterium hallii group,Anaerostipes hadrus,and Dorea longicatena,were positively correlated with FBG.Bacteroides vulgatus ATCC8482,Bacteroides ovatus,the Eubacterium hallii group,and Anaerostipes hadrus,either alone or in combination,could be used as noninvasive diagnostic biomarkers to discriminate children with T1DM from healthy controls.In addition,the functional changes in the T1DM-associated fecal microbiota also suggest that these fecal microbes were associated with altered functions and metabolic activities,such as glycan biosynthesis and metabolism and lipid metabolism,which might play vital roles in the pathogenesis and development of T1DM.CONCLUSION Our present comprehensive investigation of the T1DM-associated fecal microbiota provides novel insights into the pathogenesis of the disease and sheds light on the diagnosis and treatment of T1DM.展开更多
目的:观察益气化聚方联合硫辛酸对2型糖尿病(T2DM)患者血糖、代谢指标及周围神经病变的影响。方法:选取2020年8月至2022年12月上海中医药大学附属岳阳中西医结合医院收治的T2DM患者120例作为研究对象,采用随机数字表法分为对照组和观察...目的:观察益气化聚方联合硫辛酸对2型糖尿病(T2DM)患者血糖、代谢指标及周围神经病变的影响。方法:选取2020年8月至2022年12月上海中医药大学附属岳阳中西医结合医院收治的T2DM患者120例作为研究对象,采用随机数字表法分为对照组和观察组,每组60例。对照组给予硫辛酸治疗,观察组在对照组基础上加用益气化聚方治疗。比较2组患者在治疗后的临床疗效、血糖变化、代谢相关指标、周围神经病变及治疗期间不良反应发生情况。结果:治疗后,观察组患者治疗总有效率高于对照组,且高低密度脂蛋白胆固醇(HDL-C)水平、总神经运动神经传导速度(MNCV)及感觉神经传导速度(SNCV)评分(正中神经和腓总神经)显著升高,差异有统计学意义(P<0.05),空腹血糖(FPG)、餐后2 h血糖(2 h PG)、总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、胰岛素抵抗指数(HOMA-IR)与对照组比较显著降低(P<0.05)。观察组治疗期间出现血小板功能异常2例、脸色苍白1例,对照组治疗期间出现血小板功能异常2例、脸色苍白2例,2组治疗后不良反应发生情况差异无统计学意义。结论:采用益气化聚方联合硫辛酸治疗T2DM可更明显改善周围神经病变,降低血糖,控制血脂,且安全性良好。展开更多
Objective:To explore demographic and metabolic factors associated with increased alanine aminotransferase(ALT)activity in non-diabetic non-alcoholic fatty liver disease(NAFLD)patients.Methods:Overall 372 patients who ...Objective:To explore demographic and metabolic factors associated with increased alanine aminotransferase(ALT)activity in non-diabetic non-alcoholic fatty liver disease(NAFLD)patients.Methods:Overall 372 patients who consecutively attended to Gastroenterology Clinic of Baqiyatallah University of Medical Sciences,Tehran,Iran awere diagnosed as NAFLD entered into analysis.Exclusion criteria were having diabetes mellitus and fasting blood glucose over126 mg/dL,active hepatitis B virus infection,having hepatitis C virus positive serology,and to be under corticosteroid therapy.ALT levels were considered pathologically high when it was over30 IU/L for men and over 19 IU/L for women.Results:Bivariate analyses using t test and chisquare test showed that patients with pathologically augmented ALT levels had significantly higher NAFLD grades in their ultrasonographic evaluations(P=0.003).Moreover,these patients represented significantly higher homeostatic model assessment levels(P=0.003),levels of serum insulin(P=0.002),fasting blood glucose(P<0.001),and uric acid(P=0.02).The prevalence of insulin resistance was also higher in patients with increased serum ALT concentrations.Multifactorial logistic regression models showed that ultrasonographic grading of NAFLD(P=0.027)and insulin resistance(P=0.013)were the only variables significantly associated with abnormal ALT levels.Conclusions:This study shows that the associations of increased ALT serum levels in NAFLD patients are different from what are supposed before.By excluding diabetic patients from our population,we find that increased ALT levels are not associated with dyslipidemias but are independently associated with insulin resistance and NAFLD grading on ultrasonographic evaluations.Further studies are needed to confirm our results.展开更多
目的探讨脂联素(ADPN)/瘦素(LEP)比值作为脂肪组织功能障碍生物标志物的应用。方法选择2021年1—12月在江苏省太湖疗养院进行体检的456名体检者作为研究对象,测量身高、体质量、体质量指数(BMI),腰围、体脂率等身体指标,检测ADPN、LEP...目的探讨脂联素(ADPN)/瘦素(LEP)比值作为脂肪组织功能障碍生物标志物的应用。方法选择2021年1—12月在江苏省太湖疗养院进行体检的456名体检者作为研究对象,测量身高、体质量、体质量指数(BMI),腰围、体脂率等身体指标,检测ADPN、LEP、三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、尿酸(UA)、超敏C-反应蛋白(hs-CRP)、葡萄糖(GLU)、同型半胱氨酸(Hcy)、胰岛素(INS)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、血清淀粉样蛋白A(SAA)水平,计算ADPN/LEP比值。根据ADPN/LEP比值将研究对象分为ADPN/LEP>1.0组(78例),ADPN/LEP 0.5~1.0组(77例),ADPN/LEP<0.5组(301例),分析并比较各组体格检查和实验室检查指标的变化及相关性;比较肥胖、糖尿病、代谢综合征(MS)患者与正常BMI、血糖正常、非MS受检者的ADPN/LEP比值差异;采用Pearson相关分析法分析ADPN/LEP比值与SAA的相关性。结果ADPN/LEP<0.5组的体脂率、BMI、腰围及TG、LDL-C、UA、hs-CRP、GLU、Hcy、INS、ALT水平均明显高于ADPN/LEP>1.0组和ADPN/LEP0.5~1.0组,HDL-C水平明显低于ADPN/LEP>1.0组和ADPN/LEP 0.5~1.0组[体脂率:(50.6±8.2)%比(24.6±6.9)%、(32.8±7.2)%,BMI(kg/m2):31.8±2.1比22.7±2.5、26.9±2.3,腰围(cm):119±13比77±10、95±15,TG(mmol/L):1.58±1.20比0.87±0.46、1.07±0.70,LDL-C(mmol/L):3.31±0.93比2.87±0.85、3.15±0.91,U A(μm o l/L):362±83比247±65、303±83,h s-C R P(m g/L):7.8±6.8比1.5±1.1、3.0±2.2,G L U(m m o l/L):5.9±1.5比5.1±0.9、5.5±1.5,H c y(μm o l/L):10.6±5.0比6.9±2.3、7.9±2.4,I N S(m U/L):17.3±7.3比4.3±3.1、5.2±3.2,ALT(U/L):30±18比13±8、17±14,HDL-C(mmol/L):1.24±0.31比1.68±0.41、1.58±0.44,均P<0.05]。正常BMI组、血糖正常组和非MS组分别有13%、56%和28%的受检者ADPN/LEP<0.5;而肥胖组、糖尿病组、MS组分别有93%、85%和87%的受检者ADPN/LEP<0.5。ADPN/LEP比值与SAA水平呈负相关(r=-0.21,P=0.005)。结论ADPN/LEP比值是脂肪组织功能障碍的可靠生物标志物,ADPN/LEP比值<0.5表明患心血管疾病风险明显增加。展开更多
基金National Natural Science Foundation of China,No.31700800,No.81771724,and No.81790631National S&T Major Project of China,No.2018YFC2000500.
文摘BACKGROUND Gut microbiota dysbiosis is reportedly actively involved in autoimmune diseases such as type 1 diabetes mellitus(T1DM).However,the alterations in the gut microbiota and their correlation with fasting blood glucose(FBG)in Chinese children with T1DM remain unclear.AIM To investigate alterations in the gut microbiota in Chinese children with T1DM and their associations with clinical indicators.METHODS Samples from 51 children with T1DM and 47 age-matched and gender-matched healthy controls were obtained,to explore the structural and functional alterations in the fecal microbiota.The V3-V4 regions of the 16S rRNA gene were sequenced on a MiSeq instrument,and the association with FBG were analyzed.RESULTS We found that the bacterial diversity was significantly increased in the T1DMassociated fecal microbiota,and changes in the microbial composition were observed at different taxonomic levels.The T1DM-reduced differential taxa,such as Bacteroides vulgatus ATCC8482,Bacteroides ovatus,Bacteroides xylanisolvens,and Flavonifractor plautii,were negatively correlated with FBG,while the T1DMenriched taxa,such as Blautia,Eubacterium hallii group,Anaerostipes hadrus,and Dorea longicatena,were positively correlated with FBG.Bacteroides vulgatus ATCC8482,Bacteroides ovatus,the Eubacterium hallii group,and Anaerostipes hadrus,either alone or in combination,could be used as noninvasive diagnostic biomarkers to discriminate children with T1DM from healthy controls.In addition,the functional changes in the T1DM-associated fecal microbiota also suggest that these fecal microbes were associated with altered functions and metabolic activities,such as glycan biosynthesis and metabolism and lipid metabolism,which might play vital roles in the pathogenesis and development of T1DM.CONCLUSION Our present comprehensive investigation of the T1DM-associated fecal microbiota provides novel insights into the pathogenesis of the disease and sheds light on the diagnosis and treatment of T1DM.
文摘目的:观察益气化聚方联合硫辛酸对2型糖尿病(T2DM)患者血糖、代谢指标及周围神经病变的影响。方法:选取2020年8月至2022年12月上海中医药大学附属岳阳中西医结合医院收治的T2DM患者120例作为研究对象,采用随机数字表法分为对照组和观察组,每组60例。对照组给予硫辛酸治疗,观察组在对照组基础上加用益气化聚方治疗。比较2组患者在治疗后的临床疗效、血糖变化、代谢相关指标、周围神经病变及治疗期间不良反应发生情况。结果:治疗后,观察组患者治疗总有效率高于对照组,且高低密度脂蛋白胆固醇(HDL-C)水平、总神经运动神经传导速度(MNCV)及感觉神经传导速度(SNCV)评分(正中神经和腓总神经)显著升高,差异有统计学意义(P<0.05),空腹血糖(FPG)、餐后2 h血糖(2 h PG)、总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、胰岛素抵抗指数(HOMA-IR)与对照组比较显著降低(P<0.05)。观察组治疗期间出现血小板功能异常2例、脸色苍白1例,对照组治疗期间出现血小板功能异常2例、脸色苍白2例,2组治疗后不良反应发生情况差异无统计学意义。结论:采用益气化聚方联合硫辛酸治疗T2DM可更明显改善周围神经病变,降低血糖,控制血脂,且安全性良好。
基金financially supported by Baqiyatallah University of Medical Sciences
文摘Objective:To explore demographic and metabolic factors associated with increased alanine aminotransferase(ALT)activity in non-diabetic non-alcoholic fatty liver disease(NAFLD)patients.Methods:Overall 372 patients who consecutively attended to Gastroenterology Clinic of Baqiyatallah University of Medical Sciences,Tehran,Iran awere diagnosed as NAFLD entered into analysis.Exclusion criteria were having diabetes mellitus and fasting blood glucose over126 mg/dL,active hepatitis B virus infection,having hepatitis C virus positive serology,and to be under corticosteroid therapy.ALT levels were considered pathologically high when it was over30 IU/L for men and over 19 IU/L for women.Results:Bivariate analyses using t test and chisquare test showed that patients with pathologically augmented ALT levels had significantly higher NAFLD grades in their ultrasonographic evaluations(P=0.003).Moreover,these patients represented significantly higher homeostatic model assessment levels(P=0.003),levels of serum insulin(P=0.002),fasting blood glucose(P<0.001),and uric acid(P=0.02).The prevalence of insulin resistance was also higher in patients with increased serum ALT concentrations.Multifactorial logistic regression models showed that ultrasonographic grading of NAFLD(P=0.027)and insulin resistance(P=0.013)were the only variables significantly associated with abnormal ALT levels.Conclusions:This study shows that the associations of increased ALT serum levels in NAFLD patients are different from what are supposed before.By excluding diabetic patients from our population,we find that increased ALT levels are not associated with dyslipidemias but are independently associated with insulin resistance and NAFLD grading on ultrasonographic evaluations.Further studies are needed to confirm our results.
文摘目的探讨脂联素(ADPN)/瘦素(LEP)比值作为脂肪组织功能障碍生物标志物的应用。方法选择2021年1—12月在江苏省太湖疗养院进行体检的456名体检者作为研究对象,测量身高、体质量、体质量指数(BMI),腰围、体脂率等身体指标,检测ADPN、LEP、三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、尿酸(UA)、超敏C-反应蛋白(hs-CRP)、葡萄糖(GLU)、同型半胱氨酸(Hcy)、胰岛素(INS)、丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、血清淀粉样蛋白A(SAA)水平,计算ADPN/LEP比值。根据ADPN/LEP比值将研究对象分为ADPN/LEP>1.0组(78例),ADPN/LEP 0.5~1.0组(77例),ADPN/LEP<0.5组(301例),分析并比较各组体格检查和实验室检查指标的变化及相关性;比较肥胖、糖尿病、代谢综合征(MS)患者与正常BMI、血糖正常、非MS受检者的ADPN/LEP比值差异;采用Pearson相关分析法分析ADPN/LEP比值与SAA的相关性。结果ADPN/LEP<0.5组的体脂率、BMI、腰围及TG、LDL-C、UA、hs-CRP、GLU、Hcy、INS、ALT水平均明显高于ADPN/LEP>1.0组和ADPN/LEP0.5~1.0组,HDL-C水平明显低于ADPN/LEP>1.0组和ADPN/LEP 0.5~1.0组[体脂率:(50.6±8.2)%比(24.6±6.9)%、(32.8±7.2)%,BMI(kg/m2):31.8±2.1比22.7±2.5、26.9±2.3,腰围(cm):119±13比77±10、95±15,TG(mmol/L):1.58±1.20比0.87±0.46、1.07±0.70,LDL-C(mmol/L):3.31±0.93比2.87±0.85、3.15±0.91,U A(μm o l/L):362±83比247±65、303±83,h s-C R P(m g/L):7.8±6.8比1.5±1.1、3.0±2.2,G L U(m m o l/L):5.9±1.5比5.1±0.9、5.5±1.5,H c y(μm o l/L):10.6±5.0比6.9±2.3、7.9±2.4,I N S(m U/L):17.3±7.3比4.3±3.1、5.2±3.2,ALT(U/L):30±18比13±8、17±14,HDL-C(mmol/L):1.24±0.31比1.68±0.41、1.58±0.44,均P<0.05]。正常BMI组、血糖正常组和非MS组分别有13%、56%和28%的受检者ADPN/LEP<0.5;而肥胖组、糖尿病组、MS组分别有93%、85%和87%的受检者ADPN/LEP<0.5。ADPN/LEP比值与SAA水平呈负相关(r=-0.21,P=0.005)。结论ADPN/LEP比值是脂肪组织功能障碍的可靠生物标志物,ADPN/LEP比值<0.5表明患心血管疾病风险明显增加。
