A novel scheme to suppress both stimulated Brillouin scattering(SBS) and stimulated Raman scattering(SRS) by combining an alternating frequency(AF) laser and a transverse magnetic field is proposed. The AF laser allow...A novel scheme to suppress both stimulated Brillouin scattering(SBS) and stimulated Raman scattering(SRS) by combining an alternating frequency(AF) laser and a transverse magnetic field is proposed. The AF laser allows the laser frequency to change discretely and alternately over time. The suppression of SBS is significant as long as the AF difference is greater than the linear growth rate of SBS or the alternating time of the laser frequency is shorter than the linear growth time of SBS. However, the AF laser proves ineffective in suppressing SRS, which usually has a much higher linear growth rate than SBS. To remedy that, a transverse magnetic field is included to suppress the SRS instability. The electrons trapped in the electron plasma waves(EPWs) of SRS can be accelerated by the surfatron mechanism in a transverse magnetic field and eventually detrapped. While continuously extracting energy from EPWs, the EPWs are dissipated and the kinetic inflation of SRS is suppressed. The one-dimensional particle-in-cell simulation results show that both SBS and SRS can be effectively suppressed by combining the AF laser with a transverse magnetic field with tens of Tesla. The total reflectivity can be dramatically reduced by more than one order of magnitude. These results provide a potential reference for controlling SBS and SRS under the related parameters of inertial confinement fusion.展开更多
Because the breast cancer is an important factor that threatens women's lives and health,early diagnosis is helpful for disease screening and a good prognosis.Exosomes are nanovesicles,secreted from cells and othe...Because the breast cancer is an important factor that threatens women's lives and health,early diagnosis is helpful for disease screening and a good prognosis.Exosomes are nanovesicles,secreted from cells and other body fluids,which can reflect the genetic and phenotypic status of parental cells.Compared with other methods for early diagnosis of cancer(such as circulating tumor cells(CTCs)and circulating tumor DNA),exosomes have a richer number and stronger biological stability,and have great potential in early diagnosis.Thus,it has been proposed as promising biomarkers for diagnosis of early-stage cancer.However,distinguishing different exosomes remain is a major biomedical challenge.In this paper,we used predictive Convolutional Neural model to detect and analyze exosomes of normal and cancer cells with surface-enhanced Raman scattering(SERS).As a result,it can be seen from the SERS spectra that the exosomes of MCF-7,MDA-MB-231 and MCF-10A cells have similar peaks(939,1145 and 1380 cm^(-1)).Based on this dataset,the predictive model can achieve 95%accuracy.Compared with principal component analysis(PCA),the trained CNN can classify exosomes from different breast cancer cells with a superior performance.The results indicate that using the sensitivity of Raman detection and exosomes stable presence in the incubation period of cancer cells,SERS detection combined with CNN screening may be used for the early diagnosis of breast cancer in the future.展开更多
In-situ measurement of internal solitary waves(ISWs)is complicated in the ocean due to their randomness.At present,the ISWs are mainly detected by the chain structure of conductivity-temperature-depth systems(CTDs)or ...In-situ measurement of internal solitary waves(ISWs)is complicated in the ocean due to their randomness.At present,the ISWs are mainly detected by the chain structure of conductivity-temperature-depth systems(CTDs)or temperature sensors.The high cost limits the spatial resolution,which ultimately affects the measuring accuracy of the ISW amplitude.In this paper,we developed an experimental measurement system for detecting ISWs based on the stimulated Raman scattering in distributed optical fibers.This system has the advantages of high precision,low cost,and easy operation.The experimental results show that the system is consistent with CTDs in the measurement of vertical ocean temperature variation.The spatial resolution of the system can reach 1.0 m and the measuring accuracy of temperature is 0.2℃.We successfully detected 3 ISWs by the system in the South China Sea and two optical remote sensing images collected on May 18,2021,the same day of two detected ISWs,verify the occurrence of the measured ISWs.We used the image pairs method to calculate the phase velocity of ISW and the result is 1.71 ms^(-1).By extracting the distances between wave packets,it can be found that the semi-diurnal tide generates the detected ISWs.The impact of the tidal current velocity on the ISW in amplitude is undeniable.Undoubtedly,the system has a great application prospect for detecting ISWs and other dynamic phenomena in the ocean.展开更多
Beneting from the developments of advanced optical microscopy techniques,the mysteries of biological functions at the cellular and subcellular levels have been continuously revealed.Stimulated Raman scattering(SRS)mic...Beneting from the developments of advanced optical microscopy techniques,the mysteries of biological functions at the cellular and subcellular levels have been continuously revealed.Stimulated Raman scattering(SRS)microscopy is a rapidly growing technique that has attracted broad attentions and become a powerful tool for biology and biomedicine,largely thanks to its chemical specicity,high sensitivity and fast image speed.This review paper introduces the principles of SRS,discusses the technical developments and implementations of SRS microscopy,then highlights and summarizes its applications on biological cellular machinery andnally shares our visions of potential breakthroughs in the future.展开更多
With the continuous discovery and research of predictive cancer-related biomarkers,liquid biopsy shows great potential in cancer diagnosis.Surface-enhanced Raman scattering(SERS)and microfluidic technology have receiv...With the continuous discovery and research of predictive cancer-related biomarkers,liquid biopsy shows great potential in cancer diagnosis.Surface-enhanced Raman scattering(SERS)and microfluidic technology have received much attention among the various cancer biomarker detection methods.The former has ultrahigh detection sensitivity and can provide a unique fingerprint.In contrast,the latter has the characteristics of miniaturization and integration,which can realize accurate control of the detection samples and high-throughput detection through design.Both have the potential for point-of-care testing(POCT),and their combination(lab-on-a-chip SERS(LoC-SERS))shows good compatibility.In this paper,the basic situation of circulating proteins,circulating tumor cells,exosomes,circulating tumor DNA(ctDNA),and microRNA(miRNA)in the diagnosis of various cancers is reviewed,and the detection research of these biomarkers by the LoC-SERS platform in recent years is described in detail.At the same time,the challenges and future development of the platform are discussed at the end of the review.Summarizing the current technology is expected to provide a reference for scholars engaged in related work and interested in this field.展开更多
Cancer cells dysregulate lipid metabolism to accelerate energy production and biomolecule synthesis for rapid growth.Lipid metabolism is highly dynamic and intrinsically heterogeneous at the single cell level.Although...Cancer cells dysregulate lipid metabolism to accelerate energy production and biomolecule synthesis for rapid growth.Lipid metabolism is highly dynamic and intrinsically heterogeneous at the single cell level.Although°uorescence microscopy has been commonly used for cancer research,bulky°uorescent probes can hardly label small lipid molecules without perturbing their biological activities.Such a challenge can be overcome by coherent Raman scattering(CRS)microscopy,which is capable of chemically selective,highly sensitive,submicron resolution and high-speed imaging of lipid molecules in single live cells without any labeling.Recently developed hyperspectral and multiplex CRS microscopy enables quantitative mapping of various lipid metabolites in situ.Further incorporation of CRS microscopy with Raman tags greatly increases molecular selectivity based on the distinct Raman peaks well separated from the endogenous cellular background.Owing to these unique advantages,CRS microscopy sheds new insights into the role of lipid metabolism in cancer development and progression.This review focuses on the latest applications of CRS microscopy in the study of lipid metabolism in cancer.展开更多
COVID-19 has devastated numerous nations around the world and has overburdened numerous healthcare systems,which has also caused the loss of livelihoods due to prolonged shutdowns and further led to a cascading effect...COVID-19 has devastated numerous nations around the world and has overburdened numerous healthcare systems,which has also caused the loss of livelihoods due to prolonged shutdowns and further led to a cascading effect on the global economy.COVID-19 infections have an incubation period of 2–7 days,but 40 to 45%of cases are asymptomatic or show mild to moderate respiratory symptoms after the period due to subclinical lung abnormalities,making it more likely to spread the pandemic disease.To restrict the spread of the virus,on-site diagnosis methods that are quicker,more precise,and easily accessible are required.Rapid Antigen Detection Tests and Polymerase Chain Reaction tests are currently the primary methods used to determine the presence of COVID-19 viruses.These tests are typically time-consuming,not accurate,and,more importantly,not available to everyone.Hence,in this review and hypothesis,we proposed equipment that employs the properties of photonics to improve the detection of COVID-19 viruses by taking the advantage of typical binding of coronavirus with angiotensin-converting enzyme 2(ACE2)receptors.This hypothetical model would combine Surface-Enhanced Raman Scattering(SERS)and Fluorescence Resonance Energy Transfer(FRET)to provide great flexibility,high sensitivities,and enhanced accessibility.展开更多
Surface enhanced Raman scattering(SERS)is an efficient technique to detect low concentration molecules.In this work,periodical silicon nanowires(Si NWs)integrated with metal-insulator-metal(MIM)layers are employed as ...Surface enhanced Raman scattering(SERS)is an efficient technique to detect low concentration molecules.In this work,periodical silicon nanowires(Si NWs)integrated with metal-insulator-metal(MIM)layers are employed as SERS substrates.Laser interference lithography(LIL)combined with reactive ion etching(RIE)is used to fabricate large-area periodic nanostructures,followed by decorating the MIM layers.Compared to MIM disks array on Si surface,the SERS enhancement factor(EF)of the MIM structures on the Si NWs array can be increased up to 5 times,which is attributed to the enhanced electric field at the boundary of the MIM disks.Furthermore,high density of nanoparticles and nanogaps serving as hot spots on sidewall surfaces also contribute to the enhanced SERS signals.Via changing the thickness of the insulator layer,the plasmonic resonance can be tuned,which provides a new localized surface plasmon resonance(LSPR)characteristic for SERS applications.展开更多
Rapid and simple detections of two kinds of prohibited fish drugs, crystal violet (CV) and malachite green (MG), were accomplished by surface-enhanced Raman scattering (SERS). Based on the optimized Au/cicada wi...Rapid and simple detections of two kinds of prohibited fish drugs, crystal violet (CV) and malachite green (MG), were accomplished by surface-enhanced Raman scattering (SERS). Based on the optimized Au/cicada wing, the detectable concentration of CV/MG can reach 10-7 M, and the linear logarithmic quantitative relationship curves between log/and logC allows for the determination of the unknown concentration of CV/MG solution. The detection of these two analytes in real environment was also achieved, demonstrating the application potential of SERS in the fast screening of the prohibited fish drugs, which is of great benefit for food safety and environmental monitoring.展开更多
In broadband coherent anti-Stokes Raman scattering (CARS) spectroscopy with supercontinuum (SC), the simultaneously detectable spectral coverage is limited by the spectral continuity and the simultaneity of variou...In broadband coherent anti-Stokes Raman scattering (CARS) spectroscopy with supercontinuum (SC), the simultaneously detectable spectral coverage is limited by the spectral continuity and the simultaneity of various spectral components of SC in an enough bandwidth. By numerical simulations, the optimal experimental conditions for improving the SC are obtained. The broadband time-resolved CARS spectrography based on the SC with required temporal and spectral distributions is realised. The global molecular vibrational spectrum with well suppressed nonresonant background noise can be obtained in a single measurement. At the same time, the measurements of dephasing times of various molecular vibrational modes can be conveniently achieved from intensities of a sequence of time-resolved CARS signals. It will be more helpful to provide a complete picture of molecular vibrations, and to exhibit a potential to understand not only both the solvent dynamics and the solute-solvent interactions, but also the mechanisms of chemical reactions in the fields of biology, chemistry and material science.展开更多
Surface-enhanced resonance Raman scattering (SERRS) of Rhodamine 6G (R6G) adsorbed on colloidal silver clusters has been studied. Based on the great enhancement of the Raman signal and the quench of the fluorescen...Surface-enhanced resonance Raman scattering (SERRS) of Rhodamine 6G (R6G) adsorbed on colloidal silver clusters has been studied. Based on the great enhancement of the Raman signal and the quench of the fluorescence, the SERRS spectra of R6G were recorded for the samples of dye colloidal solution with different concentrations. Spectral inhomogeneity behaviours from single molecules in the dried sample films were observed with complementary evidences, such as spectral polarization, spectral diffusion, intensity fluctuation of vibrational lines and even "breathing" of the molecules. Sequential spectra observed from a liquid sample with an average of 0.3 dye molecules in the probed volume exhibited the expected Poisson distribution for actually measuring 0, 1 or 2 molecules. Difference between the SERRS spectra of R6G excited by linearly and circularly polarized light were experimentally measured.展开更多
The free carrier density and mobility in n-type 4H-SiC substrates and epilayers were determined by accurately analysing the frequency shift and the full-shape of the longitudinal optic phono-plasmon coupled (LOPC) m...The free carrier density and mobility in n-type 4H-SiC substrates and epilayers were determined by accurately analysing the frequency shift and the full-shape of the longitudinal optic phono-plasmon coupled (LOPC) modes, and compared with those determined by Hall-effect measurement and that provided by the vendors. The transport properties of thick and thin 4H-SiC epilayers grown in both vertical and horizontal reactors were also studied. The free carrier density ranges between 2× 10^18 cm^-3 and 8× 10^18 cm^-3with a carrier mobility of 30-55 cm2/(V.s) for ntype 4H-SiC substrates and 1× 10^16 -3× 10^16 cm^-3 with mobility of 290-490 cm2/(V.s) for both thick and thin 4H-SiC epilayers grown in a horizontal reactor, while thick 4H-SiC epilayers grown in vertical reactor have a slightly higher carrier concentration of around 8.1×10^16 cm^-3 with mobility of 380 cm2/(V.s). It was shown that Raman spectroscopy is a potential technique for determining the transport properties of 4H-SiC wafers with the advantage of being able to probe very small volumes and also being non-destructive. This is especially useful for future mass production of 4H-SiC epi-wafers.展开更多
The high-frequency edge of the first-order Raman mode of diamond reflects the stress state at the culet of anvil, and is often used for the pressure calibration in diamond anvil cell(DAC) experiments. Here we point ou...The high-frequency edge of the first-order Raman mode of diamond reflects the stress state at the culet of anvil, and is often used for the pressure calibration in diamond anvil cell(DAC) experiments. Here we point out that the high-frequency edge of the diamond Raman phonon corresponds to the Brillouin zone(BZ) center Γ point as a function of pressure. The diamond Raman pressure gauge relies on the stability of crystal lattice of diamond under high stress. Upon the diamond anvil occurs failure under the uniaxial stress(197 GPa), the loss of intensity of the first-order Raman phonon and a stressdependent broad Raman band centered at 600 cm^(-1) are observed, which is associated with a strain-induced local mode corresponding to the BZ edge phonon of the L1 transverse acoustic phonon branch.展开更多
We proposed a facile and rapid method for preparing silica-silver core-shell(SSCS) substrates to use Ag electroless plating on SiO2@Au-seed particles.UV-Vis-NIR absorption spectrometer and SEM were employed to monit...We proposed a facile and rapid method for preparing silica-silver core-shell(SSCS) substrates to use Ag electroless plating on SiO2@Au-seed particles.UV-Vis-NIR absorption spectrometer and SEM were employed to monitor the reaction process of the formation of Ag on the surfaces of silica beads,and the optical resonance of the substrate could shift from visible to NIR region.It has been found that surface-enhanced Raman scattering(SERS) enhancement changes with the electroless plating time and the SSCS substrate with the plating time of 90 s(90SSCS) shows the strongest SERS response under the laser excitation at 514.5 nm.Signals collected over multiple spots and substrate of rhodamine 6G(R6G) resulted in a relative standard deviation(RSD) of 9.75%.The calculated enhancement factor(EF) was approximately 105 "106.SSCS substrate exhibits high SERS performance,which is due to electromagnetic SERS enhancement with additional localization field within closely packed Ag nanoparticles decorated on the SiO2 nanoparticles.And this substrate presents tunable and broad localized surface plasmon resonance(LSPR),so this method may open a new way for SERS studies with other laser excitation.展开更多
Femtosecond time-resolved coherent anti-Stokes Raman scattering (CARS) spectroscopy is used to investigate gaseous molecular dynamics. Due to the spectrally broad laser pulses, usually poorly resolved spectra result...Femtosecond time-resolved coherent anti-Stokes Raman scattering (CARS) spectroscopy is used to investigate gaseous molecular dynamics. Due to the spectrally broad laser pulses, usually poorly resolved spectra result from this broad spectroscopy. However, it can be demonstrated that by the electronic resonance enhancement optimization control a selective excitation of specific vibrational mode is possible. Using an electronically resonance-enhanced effect, iodine molecule specific CARS spectroscopy can be obtained from a mixture of iodine-air at room temperature and a pressure of 1 atm (corresponding to a saturation iodine vapour as low as about 35 Pa). The dynamics on either the electronically excited state or the ground state of iodine molecules obtained is consistent with previous studies (vacuum, heated and pure iodine) in the femtoseeond time resolved CARS spectroscopy, showing that an effective method of suppressing the non-resonant CARS background and other interferences is demonstrated.展开更多
Stimulated Raman scattering(SRS)microscopy has the ability of noninvasive imaging of specific chemical bonds and been increasingly used in biomedicine in recent years.Two pulsed Gaussian beams are used in traditional ...Stimulated Raman scattering(SRS)microscopy has the ability of noninvasive imaging of specific chemical bonds and been increasingly used in biomedicine in recent years.Two pulsed Gaussian beams are used in traditional SRS microscopes,providing with high lateral and axial spatial resolution.Because of the tight focus of the Gaussian beam,such an SRS microscopy is difficult to be used for imaging deep targets in scattering tissues.The SRS microscopy based on Bessel beams can solve the imaging problem to a certain extent.Here,we establish a theoretical model to calculate the SRS signal excited by two Bessel beams by integrating the SRS signal generation theory with the fractal propagation method.The fractal model of refractive index turbulence is employed to generate the scattering tissues where the light transport is modeled by the beam propagation method.We model the scattering tissues containing chemicals,calculate the SRS signals stimulated by two Bessel beams,discuss the influence of the fractal model parameters on signal generation,and compare them with those generated by the Gaussian beams.The results show that,even though the modeling parameters have great influence on SRS signal generation,the Bessel beams-based SRS can generate signals in deeper scattering tissues.展开更多
Raman spectrum is a powerful analytical tool for determining the chemical information of compounds. In this study, we obtained analytical results of chlorophenols(CPs) molecules including 4-chlorophenol(4-CP), 2,6...Raman spectrum is a powerful analytical tool for determining the chemical information of compounds. In this study, we obtained analytical results of chlorophenols(CPs) molecules including 4-chlorophenol(4-CP), 2,6-dich- lorophenol(2,6-DCP) and 2,4,6-trichlorophenol(2,4,6-TCP) on the surface of Ag dendrites by surface-enhanced Raman scattering(SERS) spectra. SEM images indicate that the SERS substrate of Ag dendrites is composed of a large number of polygonal nanocrystallites, which self-assembled into a 3D hierarchical structure. It was found that there were distinct differences for those three molecules from Raman and SERS spectra. This indicates that SERS could be a new tool of detection technique regarding trace amounts of CPs.展开更多
Stimulated raman scattering (SRS) is an effective method for expanding the spectral range of high power lasers, especially in the regime of near IR and middle IR. We report the SRS of high pressure H2 with a multipl...Stimulated raman scattering (SRS) is an effective method for expanding the spectral range of high power lasers, especially in the regime of near IR and middle IR. We report the SRS of high pressure H2 with a multiple-pass cell configuration. The SRS with the multiple-pass cell configuration is found to be very efficient for reduction of threshold of the first Stokes (S1). Due to the coherent SRS (CSRS) process, the multiple-pass cell configuration is more effective for reduction of the threshold for the second Stokes (S2) SRS and for increasing the conversion efficiency of S2. This contributes to the relatively low conversion efficiency of S1 for the multiple-pass cell configuration. Multiple-pass cell SRS is also found to be very effective for improving the beam quality and the stability of S1.展开更多
The effect of solvent on surface enhanced Raman scattering [SERS) of colloidal silver has been studied. Experiments show that the intensity of SERS is related to the polarity and molecular constitution of the solvent....The effect of solvent on surface enhanced Raman scattering [SERS) of colloidal silver has been studied. Experiments show that the intensity of SERS is related to the polarity and molecular constitution of the solvent. The influence of solvent is due to the change of the adsorption quantity and adsorption intensity.展开更多
Gold nanorods with aspect ratios of from 1 (particles) to 31.6 were synthesized by the seed-mediated method and packed in a highly ordered structure on a large scale on silicon substrates through capillary force ind...Gold nanorods with aspect ratios of from 1 (particles) to 31.6 were synthesized by the seed-mediated method and packed in a highly ordered structure on a large scale on silicon substrates through capillary force induced self-assembly behaviour during solvent evaporation. The gold nanorod surface exhibits a strong enhancing effect on Raman scattering spectroscopy. The enhancement of Raman scattering for two model molecules (2-naphthalenethiol and rhodamine 6C) is about 5-6 orders of magnitude. By changing the aspect ratio of the Au nanorods, we found that the enhancement factors decreased with the increase of aspect ratios. The observed Raman scattering enhancement is strong and should be ascribed to the surface plasmon coupling between closely packed nanorods, which may result in huge local electromagnetic field enhancements in those confined junctions.展开更多
基金Project supported by the National Natural Science Foundation of China (Grant Nos.11975059 and 12005021)。
文摘A novel scheme to suppress both stimulated Brillouin scattering(SBS) and stimulated Raman scattering(SRS) by combining an alternating frequency(AF) laser and a transverse magnetic field is proposed. The AF laser allows the laser frequency to change discretely and alternately over time. The suppression of SBS is significant as long as the AF difference is greater than the linear growth rate of SBS or the alternating time of the laser frequency is shorter than the linear growth time of SBS. However, the AF laser proves ineffective in suppressing SRS, which usually has a much higher linear growth rate than SBS. To remedy that, a transverse magnetic field is included to suppress the SRS instability. The electrons trapped in the electron plasma waves(EPWs) of SRS can be accelerated by the surfatron mechanism in a transverse magnetic field and eventually detrapped. While continuously extracting energy from EPWs, the EPWs are dissipated and the kinetic inflation of SRS is suppressed. The one-dimensional particle-in-cell simulation results show that both SBS and SRS can be effectively suppressed by combining the AF laser with a transverse magnetic field with tens of Tesla. The total reflectivity can be dramatically reduced by more than one order of magnitude. These results provide a potential reference for controlling SBS and SRS under the related parameters of inertial confinement fusion.
基金This work was supported by the National Natural Science Foundation of China(62175071,11964032,31300691,32071399 and 61675072)the Science and Technology Project of Guangdong Province of China(2017A020215059)+2 种基金the Science and Technology Project of Guangzhou City(201904010323 and 2019050001)the Innovation Project of Graduate School of South China Normal University(2019LKXM023)Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education(Fujian Normal University)(JYG2008).
文摘Because the breast cancer is an important factor that threatens women's lives and health,early diagnosis is helpful for disease screening and a good prognosis.Exosomes are nanovesicles,secreted from cells and other body fluids,which can reflect the genetic and phenotypic status of parental cells.Compared with other methods for early diagnosis of cancer(such as circulating tumor cells(CTCs)and circulating tumor DNA),exosomes have a richer number and stronger biological stability,and have great potential in early diagnosis.Thus,it has been proposed as promising biomarkers for diagnosis of early-stage cancer.However,distinguishing different exosomes remain is a major biomedical challenge.In this paper,we used predictive Convolutional Neural model to detect and analyze exosomes of normal and cancer cells with surface-enhanced Raman scattering(SERS).As a result,it can be seen from the SERS spectra that the exosomes of MCF-7,MDA-MB-231 and MCF-10A cells have similar peaks(939,1145 and 1380 cm^(-1)).Based on this dataset,the predictive model can achieve 95%accuracy.Compared with principal component analysis(PCA),the trained CNN can classify exosomes from different breast cancer cells with a superior performance.The results indicate that using the sensitivity of Raman detection and exosomes stable presence in the incubation period of cancer cells,SERS detection combined with CNN screening may be used for the early diagnosis of breast cancer in the future.
基金National Natural Science Foundation of China(Nos.61871353,62031005)。
文摘In-situ measurement of internal solitary waves(ISWs)is complicated in the ocean due to their randomness.At present,the ISWs are mainly detected by the chain structure of conductivity-temperature-depth systems(CTDs)or temperature sensors.The high cost limits the spatial resolution,which ultimately affects the measuring accuracy of the ISW amplitude.In this paper,we developed an experimental measurement system for detecting ISWs based on the stimulated Raman scattering in distributed optical fibers.This system has the advantages of high precision,low cost,and easy operation.The experimental results show that the system is consistent with CTDs in the measurement of vertical ocean temperature variation.The spatial resolution of the system can reach 1.0 m and the measuring accuracy of temperature is 0.2℃.We successfully detected 3 ISWs by the system in the South China Sea and two optical remote sensing images collected on May 18,2021,the same day of two detected ISWs,verify the occurrence of the measured ISWs.We used the image pairs method to calculate the phase velocity of ISW and the result is 1.71 ms^(-1).By extracting the distances between wave packets,it can be found that the semi-diurnal tide generates the detected ISWs.The impact of the tidal current velocity on the ISW in amplitude is undeniable.Undoubtedly,the system has a great application prospect for detecting ISWs and other dynamic phenomena in the ocean.
基金We acknowledge the financial supports from the National Key R&D Program of China(2021YFF0502900)the National Natural Science Foundation of China(61975033)Shanghai Municipal Science and Technology Major Project No.2018SHZDZX01 and ZJLab.
文摘Beneting from the developments of advanced optical microscopy techniques,the mysteries of biological functions at the cellular and subcellular levels have been continuously revealed.Stimulated Raman scattering(SRS)microscopy is a rapidly growing technique that has attracted broad attentions and become a powerful tool for biology and biomedicine,largely thanks to its chemical specicity,high sensitivity and fast image speed.This review paper introduces the principles of SRS,discusses the technical developments and implementations of SRS microscopy,then highlights and summarizes its applications on biological cellular machinery andnally shares our visions of potential breakthroughs in the future.
基金supported by the Natural Science Foundation of Hunan Province,China(Grant No.:2021JJ80078).
文摘With the continuous discovery and research of predictive cancer-related biomarkers,liquid biopsy shows great potential in cancer diagnosis.Surface-enhanced Raman scattering(SERS)and microfluidic technology have received much attention among the various cancer biomarker detection methods.The former has ultrahigh detection sensitivity and can provide a unique fingerprint.In contrast,the latter has the characteristics of miniaturization and integration,which can realize accurate control of the detection samples and high-throughput detection through design.Both have the potential for point-of-care testing(POCT),and their combination(lab-on-a-chip SERS(LoC-SERS))shows good compatibility.In this paper,the basic situation of circulating proteins,circulating tumor cells,exosomes,circulating tumor DNA(ctDNA),and microRNA(miRNA)in the diagnosis of various cancers is reviewed,and the detection research of these biomarkers by the LoC-SERS platform in recent years is described in detail.At the same time,the challenges and future development of the platform are discussed at the end of the review.Summarizing the current technology is expected to provide a reference for scholars engaged in related work and interested in this field.
基金supported by the National Natural Science Foundation of China(Nos.91959120 and 62027824)Basic Research Program for Beijing-Tianjin-Hebei Coordination(19JCZDJC65500(Z))+1 种基金Open Project Program of Wuhan National Laboratory for Optoelectronics(No.2018WNLOKF026)Fundamental Research Funds for the Central Universities(YWF-22-L-547).
文摘Cancer cells dysregulate lipid metabolism to accelerate energy production and biomolecule synthesis for rapid growth.Lipid metabolism is highly dynamic and intrinsically heterogeneous at the single cell level.Although°uorescence microscopy has been commonly used for cancer research,bulky°uorescent probes can hardly label small lipid molecules without perturbing their biological activities.Such a challenge can be overcome by coherent Raman scattering(CRS)microscopy,which is capable of chemically selective,highly sensitive,submicron resolution and high-speed imaging of lipid molecules in single live cells without any labeling.Recently developed hyperspectral and multiplex CRS microscopy enables quantitative mapping of various lipid metabolites in situ.Further incorporation of CRS microscopy with Raman tags greatly increases molecular selectivity based on the distinct Raman peaks well separated from the endogenous cellular background.Owing to these unique advantages,CRS microscopy sheds new insights into the role of lipid metabolism in cancer development and progression.This review focuses on the latest applications of CRS microscopy in the study of lipid metabolism in cancer.
文摘COVID-19 has devastated numerous nations around the world and has overburdened numerous healthcare systems,which has also caused the loss of livelihoods due to prolonged shutdowns and further led to a cascading effect on the global economy.COVID-19 infections have an incubation period of 2–7 days,but 40 to 45%of cases are asymptomatic or show mild to moderate respiratory symptoms after the period due to subclinical lung abnormalities,making it more likely to spread the pandemic disease.To restrict the spread of the virus,on-site diagnosis methods that are quicker,more precise,and easily accessible are required.Rapid Antigen Detection Tests and Polymerase Chain Reaction tests are currently the primary methods used to determine the presence of COVID-19 viruses.These tests are typically time-consuming,not accurate,and,more importantly,not available to everyone.Hence,in this review and hypothesis,we proposed equipment that employs the properties of photonics to improve the detection of COVID-19 viruses by taking the advantage of typical binding of coronavirus with angiotensin-converting enzyme 2(ACE2)receptors.This hypothetical model would combine Surface-Enhanced Raman Scattering(SERS)and Fluorescence Resonance Energy Transfer(FRET)to provide great flexibility,high sensitivities,and enhanced accessibility.
基金financial support from A*STAR,SERC 2014 Public Sector Research Funding (PSF) Grant (SERC Project No. 1421200080)
文摘Surface enhanced Raman scattering(SERS)is an efficient technique to detect low concentration molecules.In this work,periodical silicon nanowires(Si NWs)integrated with metal-insulator-metal(MIM)layers are employed as SERS substrates.Laser interference lithography(LIL)combined with reactive ion etching(RIE)is used to fabricate large-area periodic nanostructures,followed by decorating the MIM layers.Compared to MIM disks array on Si surface,the SERS enhancement factor(EF)of the MIM structures on the Si NWs array can be increased up to 5 times,which is attributed to the enhanced electric field at the boundary of the MIM disks.Furthermore,high density of nanoparticles and nanogaps serving as hot spots on sidewall surfaces also contribute to the enhanced SERS signals.Via changing the thickness of the insulator layer,the plasmonic resonance can be tuned,which provides a new localized surface plasmon resonance(LSPR)characteristic for SERS applications.
基金Project supported by the National Basic Research Program of China(Grant No.2014CB745100)the National Natural Science Foundation of China(Grant Nos.21390202 and 21676015)the Beijing Higher Education Young Elite Teacher Project
文摘Rapid and simple detections of two kinds of prohibited fish drugs, crystal violet (CV) and malachite green (MG), were accomplished by surface-enhanced Raman scattering (SERS). Based on the optimized Au/cicada wing, the detectable concentration of CV/MG can reach 10-7 M, and the linear logarithmic quantitative relationship curves between log/and logC allows for the determination of the unknown concentration of CV/MG solution. The detection of these two analytes in real environment was also achieved, demonstrating the application potential of SERS in the fast screening of the prohibited fish drugs, which is of great benefit for food safety and environmental monitoring.
基金Project supported by the National Natural Science Foundation of China(Grant No.60627003)the Foundation for Creative Team in Institution of Higher Education of Guangdong Province,China(Grant No.06CXTD009)
文摘In broadband coherent anti-Stokes Raman scattering (CARS) spectroscopy with supercontinuum (SC), the simultaneously detectable spectral coverage is limited by the spectral continuity and the simultaneity of various spectral components of SC in an enough bandwidth. By numerical simulations, the optimal experimental conditions for improving the SC are obtained. The broadband time-resolved CARS spectrography based on the SC with required temporal and spectral distributions is realised. The global molecular vibrational spectrum with well suppressed nonresonant background noise can be obtained in a single measurement. At the same time, the measurements of dephasing times of various molecular vibrational modes can be conveniently achieved from intensities of a sequence of time-resolved CARS signals. It will be more helpful to provide a complete picture of molecular vibrations, and to exhibit a potential to understand not only both the solvent dynamics and the solute-solvent interactions, but also the mechanisms of chemical reactions in the fields of biology, chemistry and material science.
文摘Surface-enhanced resonance Raman scattering (SERRS) of Rhodamine 6G (R6G) adsorbed on colloidal silver clusters has been studied. Based on the great enhancement of the Raman signal and the quench of the fluorescence, the SERRS spectra of R6G were recorded for the samples of dye colloidal solution with different concentrations. Spectral inhomogeneity behaviours from single molecules in the dried sample films were observed with complementary evidences, such as spectral polarization, spectral diffusion, intensity fluctuation of vibrational lines and even "breathing" of the molecules. Sequential spectra observed from a liquid sample with an average of 0.3 dye molecules in the probed volume exhibited the expected Poisson distribution for actually measuring 0, 1 or 2 molecules. Difference between the SERRS spectra of R6G excited by linearly and circularly polarized light were experimentally measured.
基金supported by the National Natural Science Foundation of China (Grant No. 60876003)the Knowledge Innovation Project of Chinese Academy of Sciences (Grant Nos. Y072011000 and ISCAS2008T04)the Science and Technology Projects of the State Grid Corporation of China (ZL71-09-001)
文摘The free carrier density and mobility in n-type 4H-SiC substrates and epilayers were determined by accurately analysing the frequency shift and the full-shape of the longitudinal optic phono-plasmon coupled (LOPC) modes, and compared with those determined by Hall-effect measurement and that provided by the vendors. The transport properties of thick and thin 4H-SiC epilayers grown in both vertical and horizontal reactors were also studied. The free carrier density ranges between 2× 10^18 cm^-3 and 8× 10^18 cm^-3with a carrier mobility of 30-55 cm2/(V.s) for ntype 4H-SiC substrates and 1× 10^16 -3× 10^16 cm^-3 with mobility of 290-490 cm2/(V.s) for both thick and thin 4H-SiC epilayers grown in a horizontal reactor, while thick 4H-SiC epilayers grown in vertical reactor have a slightly higher carrier concentration of around 8.1×10^16 cm^-3 with mobility of 380 cm2/(V.s). It was shown that Raman spectroscopy is a potential technique for determining the transport properties of 4H-SiC wafers with the advantage of being able to probe very small volumes and also being non-destructive. This is especially useful for future mass production of 4H-SiC epi-wafers.
基金Project support by the National Natural Science Foundation of China(Grant No.11774247)。
文摘The high-frequency edge of the first-order Raman mode of diamond reflects the stress state at the culet of anvil, and is often used for the pressure calibration in diamond anvil cell(DAC) experiments. Here we point out that the high-frequency edge of the diamond Raman phonon corresponds to the Brillouin zone(BZ) center Γ point as a function of pressure. The diamond Raman pressure gauge relies on the stability of crystal lattice of diamond under high stress. Upon the diamond anvil occurs failure under the uniaxial stress(197 GPa), the loss of intensity of the first-order Raman phonon and a stressdependent broad Raman band centered at 600 cm^(-1) are observed, which is associated with a strain-induced local mode corresponding to the BZ edge phonon of the L1 transverse acoustic phonon branch.
基金Supported by the National Natural Science Foundation of China(Nos.20873050,20921003,20973074,20903044)the "111" Project(No.B06009)the Key Projects in the National Science & Technology Pillar Program,China(No.2007BAI38B03)
文摘We proposed a facile and rapid method for preparing silica-silver core-shell(SSCS) substrates to use Ag electroless plating on SiO2@Au-seed particles.UV-Vis-NIR absorption spectrometer and SEM were employed to monitor the reaction process of the formation of Ag on the surfaces of silica beads,and the optical resonance of the substrate could shift from visible to NIR region.It has been found that surface-enhanced Raman scattering(SERS) enhancement changes with the electroless plating time and the SSCS substrate with the plating time of 90 s(90SSCS) shows the strongest SERS response under the laser excitation at 514.5 nm.Signals collected over multiple spots and substrate of rhodamine 6G(R6G) resulted in a relative standard deviation(RSD) of 9.75%.The calculated enhancement factor(EF) was approximately 105 "106.SSCS substrate exhibits high SERS performance,which is due to electromagnetic SERS enhancement with additional localization field within closely packed Ag nanoparticles decorated on the SiO2 nanoparticles.And this substrate presents tunable and broad localized surface plasmon resonance(LSPR),so this method may open a new way for SERS studies with other laser excitation.
基金supported by the National Natural Science Foundation of China (Grant Nos.60878018 and 61008023)the Natural Scientific Research Innovation Foundation in Harbin Institute of Technology,China (Grant No.HIT.NSRIF.2009009)the Science and Technology Innovation Foundation,Harbin,China (Grant No.RC2007QN017030)
文摘Femtosecond time-resolved coherent anti-Stokes Raman scattering (CARS) spectroscopy is used to investigate gaseous molecular dynamics. Due to the spectrally broad laser pulses, usually poorly resolved spectra result from this broad spectroscopy. However, it can be demonstrated that by the electronic resonance enhancement optimization control a selective excitation of specific vibrational mode is possible. Using an electronically resonance-enhanced effect, iodine molecule specific CARS spectroscopy can be obtained from a mixture of iodine-air at room temperature and a pressure of 1 atm (corresponding to a saturation iodine vapour as low as about 35 Pa). The dynamics on either the electronically excited state or the ground state of iodine molecules obtained is consistent with previous studies (vacuum, heated and pure iodine) in the femtoseeond time resolved CARS spectroscopy, showing that an effective method of suppressing the non-resonant CARS background and other interferences is demonstrated.
基金This work was supported in part by the National Key R&D Program of China under Grant No.2018YFC0910600the National Natural Science Foundation of China under Grant Nos.81871397,81627807,11727813,91859109+2 种基金the Shaanxi Science Fund for Distinguished Young Scholars under Grant No.2020JC-27the Shaanxi Young Top-notch Talent of"Special Support Program"the Best Funded Projects for the Scientific and Technological Activities for Excellent Overseas Researchers in Shaanxi Province(2017017)..
文摘Stimulated Raman scattering(SRS)microscopy has the ability of noninvasive imaging of specific chemical bonds and been increasingly used in biomedicine in recent years.Two pulsed Gaussian beams are used in traditional SRS microscopes,providing with high lateral and axial spatial resolution.Because of the tight focus of the Gaussian beam,such an SRS microscopy is difficult to be used for imaging deep targets in scattering tissues.The SRS microscopy based on Bessel beams can solve the imaging problem to a certain extent.Here,we establish a theoretical model to calculate the SRS signal excited by two Bessel beams by integrating the SRS signal generation theory with the fractal propagation method.The fractal model of refractive index turbulence is employed to generate the scattering tissues where the light transport is modeled by the beam propagation method.We model the scattering tissues containing chemicals,calculate the SRS signals stimulated by two Bessel beams,discuss the influence of the fractal model parameters on signal generation,and compare them with those generated by the Gaussian beams.The results show that,even though the modeling parameters have great influence on SRS signal generation,the Bessel beams-based SRS can generate signals in deeper scattering tissues.
基金Supported by the National Natural Science Foundation of China(Nos.21073072, 20903044)the Scientific and Technological Development Plan Project of Jilin Province, China(No.20090546)+1 种基金the Open Project of State Key Laboratory of Superhard Materials, Chinathe Basic Research Fund of Jilin University, China
文摘Raman spectrum is a powerful analytical tool for determining the chemical information of compounds. In this study, we obtained analytical results of chlorophenols(CPs) molecules including 4-chlorophenol(4-CP), 2,6-dich- lorophenol(2,6-DCP) and 2,4,6-trichlorophenol(2,4,6-TCP) on the surface of Ag dendrites by surface-enhanced Raman scattering(SERS) spectra. SEM images indicate that the SERS substrate of Ag dendrites is composed of a large number of polygonal nanocrystallites, which self-assembled into a 3D hierarchical structure. It was found that there were distinct differences for those three molecules from Raman and SERS spectra. This indicates that SERS could be a new tool of detection technique regarding trace amounts of CPs.
基金Supported by the National Natural Science Foundation of China under Grant Nos 11304311 and 11475177
文摘Stimulated raman scattering (SRS) is an effective method for expanding the spectral range of high power lasers, especially in the regime of near IR and middle IR. We report the SRS of high pressure H2 with a multiple-pass cell configuration. The SRS with the multiple-pass cell configuration is found to be very efficient for reduction of threshold of the first Stokes (S1). Due to the coherent SRS (CSRS) process, the multiple-pass cell configuration is more effective for reduction of the threshold for the second Stokes (S2) SRS and for increasing the conversion efficiency of S2. This contributes to the relatively low conversion efficiency of S1 for the multiple-pass cell configuration. Multiple-pass cell SRS is also found to be very effective for improving the beam quality and the stability of S1.
文摘The effect of solvent on surface enhanced Raman scattering [SERS) of colloidal silver has been studied. Experiments show that the intensity of SERS is related to the polarity and molecular constitution of the solvent. The influence of solvent is due to the change of the adsorption quantity and adsorption intensity.
基金Project supported by the National Natural Science Foundation of China (Grant No.50872147)the National High Technology Research and Development Program of China (Grant No.2007AA03Z305)the Special Doctoral Foundation of the Ministry of Education of China (Grant No.20775030)
文摘Gold nanorods with aspect ratios of from 1 (particles) to 31.6 were synthesized by the seed-mediated method and packed in a highly ordered structure on a large scale on silicon substrates through capillary force induced self-assembly behaviour during solvent evaporation. The gold nanorod surface exhibits a strong enhancing effect on Raman scattering spectroscopy. The enhancement of Raman scattering for two model molecules (2-naphthalenethiol and rhodamine 6C) is about 5-6 orders of magnitude. By changing the aspect ratio of the Au nanorods, we found that the enhancement factors decreased with the increase of aspect ratios. The observed Raman scattering enhancement is strong and should be ascribed to the surface plasmon coupling between closely packed nanorods, which may result in huge local electromagnetic field enhancements in those confined junctions.