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Ramp facies in an intracratonic basin:A case study from the Upper Devonian and Lower Carboniferous in central Hunan,southern China 被引量:6
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作者 Longyi Shao Dongdong Wang Houan Cai Hao Wang Jing Lu Pengfei Zhang 《Geoscience Frontiers》 SCIE CAS 2011年第3期409-419,共11页
Detailed studies on Late Devonian to Early Carboniferous carbonate rocks in central Hunan, southern China have led to the recognition of 25 lithofacies which can be grouped into: (I) inner ramp peritidal platform, ... Detailed studies on Late Devonian to Early Carboniferous carbonate rocks in central Hunan, southern China have led to the recognition of 25 lithofacies which can be grouped into: (I) inner ramp peritidal platform, (2) inner ramp organic hank and mound, (3) mid ramp, (4) outer ramp, and (5) shelf basin facies associations. The peritidal platform facies association dominates the Zimenqiao Formation (Namurian A or late Datangian) and is characterized by gypsum and dolostone-containing sequences, indicating a peritidal platform environment. The other four facies associations dominate the Menggongao Formation (late Famennian), Liujiatang Formation (Tournaisian or Yangruanian), Shidengzi Formations (early Visean or early Datangian). Five upward-shallowing cycles were distinguished in these three Formations. The predominant facies associations developed in each Formation demonstrate an overall transgression-regression cycle in the Late Devonian to Early Carboniferous in central Hunan. The overall transgressive sequence was preserved in the Shaodong, Menggongao, and Liujiatang Formations, and the overall regressive sequence was preserved in the Liujiatang, Shidengzi, Ceshui and Zimenqiao Formations. 展开更多
关键词 Intracratonic basin Carbonate ramp Southern China Lower Carboniferous upper Devonian
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Discharge simulation and volt-second consumption analysis during ramp-up on the CFETR tokamak 被引量:3
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作者 Cheng-Yue Liu Bin Wu +6 位作者 Jin-Ping Qian Guo-Qiang Li Ya-Wei Hou Wei Wei Mei-Xia Chen Ming-Zhun Lei Yong Guo 《Chinese Physics B》 SCIE EI CAS CSCD 2020年第2期356-361,共6页
The plasma current ramp-up is an important process for tokamak discharge,which directly affects the quality of the plasma and the system resources such as volt-second consumption and plasma current profile.The China F... The plasma current ramp-up is an important process for tokamak discharge,which directly affects the quality of the plasma and the system resources such as volt-second consumption and plasma current profile.The China Fusion Engineering Test Reactor(CFETR)ramp-up discharge is predicted with the tokamak simulation code(TSC).The main plasma parameters,the plasma configuration evolution and coil current evolution are given out.At the same time,the volt-second consumption during CFETR ramp-up is analyzed for different plasma shaping times and different plasma current ramp rates dIP/dt with/without assisted heating.The results show that the earlier shaping time and the faster plasma current ramp rate with auxiliary heating will enable the volt-second to save 5%-10%.At the same time,the system ability to provide the volt-second is probably 470 V·s.These simulations will give some reference to engineering design for CFETR to some degree. 展开更多
关键词 plasma current ramp-up China Fusion Engineering Test Reactor TOKAMAK SIMULATION code volt-second CONSUMPTION
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Characteristics of an Upper Jurassic Carbonate Ramp in the Northern Amu-Darya Basin 被引量:1
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作者 Yongyao He Han Mu +2 位作者 Yuanyuan Kang Yingmin Wang Bojiang Fan 《International Journal of Geosciences》 2018年第2期148-159,共12页
Overemphasizing the value of reefs in oil and gas exploration, reef-oriented geologists explain all carbonate platform deposits using the Wilson model. In their eyes, rimmed shelves are more valuable than carbonate ra... Overemphasizing the value of reefs in oil and gas exploration, reef-oriented geologists explain all carbonate platform deposits using the Wilson model. In their eyes, rimmed shelves are more valuable than carbonate ramps. However, organic banks are excellent reservoirs generated by carbonate ramps in the study area, as verified beyond doubt through petroleum exploration, such as this thesis, which investigates the genesis, types, and distribution of carbonate deposition in the north zone of the Amu-Darya Basin. Monoclinal palaeogeomorphology and rudists suggest shallow environments. Given that oolite shoals and rudist patch reefs were observed in the study area, the depositional system is interpreted to be a carbonate ramp. The Callovian-Oxfordian stage consists of nine lithofacies: oolitic limestone, skeletal limestone, micritic limestone, bioturbated limestone, and crystalline limestone, which are grouped into three facies associations presenting outer ramp, mid-ramp, and inner ramp facies associations. Five depositional sequences can be distinguished in the Callovian-Oxfordian stage. Each third-order depositional sequence is composed of transgressive systems tracts (TST) and highstand systems tracts (HST). The TST consists of mudstones with a higher response to natural gamma rays, whereas the HST contains various types of grainstone, with subordinate dolostone. The vertical and lateral distributions of sedimentary facies, and their interpreted depositional environments, revealed a ramp exhibiting a gradual southeast-northwestward environmental change from outer ramp, mid ramp, and inner ramp carbonate facies. 展开更多
关键词 CARBONATE ramp FACIES Associations SEQUENCE Amu-Darya BASIN
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Two Dimensional Density FlUCtuation Measurements During the Non-Inductive Current Ramp-up Phase in the Compact Plasma Wall Interaction Experimental Device CPD
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作者 H.ZUSHI T.RYOUKAI +22 位作者 K.KIKUKAWA T.MORISAKI R.BHATTACHARYAY T.YOSHINAGA K.HANADA T.SAKIMURA H.IDEI K.DONO N.NISHINO H.HONMA S.TASHIMA T.MUTOH S.KUBO K.NAGASAKI M.SAKAMOTO Y.NAKASHIMA Y.HIGASHIZONO K.N.SATO K.NAKAMURA M.HASEGAWA S.KAWASAKI H.NAKASHIMA A.HIGASHIJIMA 《Plasma Science and Technology》 SCIE EI CAS CSCD 2009年第4期397-401,共5页
Two-dimensional structure of density fluctuations is examined during the current jump phase, indicating a change from the open magnetic fields to the closed ones. During the smooth current ramp-up phase the two-dimens... Two-dimensional structure of density fluctuations is examined during the current jump phase, indicating a change from the open magnetic fields to the closed ones. During the smooth current ramp-up phase the two-dimensional contour of the LiI intensity shows vertically alignment, consistent with the magnetic surfaces. At the inflection point in Ip ramp-up the LiI intensity contour becomes flat in the observation regime and then suddenly a steep gradient and higher intensity regime are formed in the vertical direction. This higher intensity corresponds to a burst of LiI waveform. According to these changes in the contour, it is found that, within ~1 ms around the burst of LiI, a low frequency coherent wave with a long wavelength rapidly grows. The relations with other signals (magnetic flux and microwave stray power) are discussed with respect to the topological change in the magnetic configuration and mode conversion of the incident electromagnetic waves. 展开更多
关键词 Li sheet beam density fluctuations current ramp-up spherical tokamak
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Current Ramp-up by Microwave Power in the LATE Device
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作者 Takashi MAEKAWA Hitoshi TANAKA Masaki UCHIDA 《Plasma Science and Technology》 SCIE EI CAS CSCD 2009年第4期394-396,共3页
In the low aspect ratio torus experiment (LATE) device, plasma current is initiated and ramped up to 20 kA solely by microwave power at the electron cyclotron (EC) range of frequency with a ramp of the external ve... In the low aspect ratio torus experiment (LATE) device, plasma current is initiated and ramped up to 20 kA solely by microwave power at the electron cyclotron (EC) range of frequency with a ramp of the external vertical field By for the radial equilibrium of plasma torus at larger currents. Measurements suggest that an energetic electron tail in the energy range of about 200 keV carries the current. The line averaged electron density is higher than the plasma cutoff density, suggesting that tail electrons might be driven by electron Bernstein waves modeconverted from the launched electromagnetic waves. 展开更多
关键词 current ramp-up MICROWAVE electron cyclotron range of frequency
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膝关节Ramp损伤的研究进展
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作者 程琛 热米拉·艾买提 +1 位作者 秦海龙 孙学斌 《创伤外科杂志》 2024年第4期305-311,共7页
Ramp损伤(RL)是膝关节内侧半月板后角与关节囊移行区域损伤,常见于慢性前交叉韧带(ACL)损伤患者,也可孤立发生。Ramp区对维持膝关节功能及保护ACL重建移植物有重要作用,膝关节MRI在诊断RL上敏感度中等但特异度较高,关节镜是诊断的金标... Ramp损伤(RL)是膝关节内侧半月板后角与关节囊移行区域损伤,常见于慢性前交叉韧带(ACL)损伤患者,也可孤立发生。Ramp区对维持膝关节功能及保护ACL重建移植物有重要作用,膝关节MRI在诊断RL上敏感度中等但特异度较高,关节镜是诊断的金标准。对RL的认识目前处于一个“再认识”的阶段,对其最佳的处理方式是近年来研究的热点。本文结合近年来研究现状,从RL的流行病学、解剖学、产生机制、分型、诊断以及手术治疗方面进行综述,以期为RL的研究及诊治提供参考。 展开更多
关键词 ramp损伤 关节镜 手术治疗
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前交叉韧带断裂合并内侧半月板Ramp区损伤的临床疗效分析
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作者 包志强 施生民 +6 位作者 曾昭勇 袁俊虎 傅煊健 陈贤艺 任庭杰 郇亚威 陈扬 《实用骨科杂志》 2024年第5期468-472,共5页
目的探讨前交叉韧带(anterior cruciate ligament,ACL)断裂合并内侧半月板后角Ramp区损伤同期治疗的临床疗效,对ACL断裂合并内侧半月板Ramp区损伤患者的手术治疗提供一些参考。方法2018年3月至2020年8月佛山市第一人民医院关节骨外科收... 目的探讨前交叉韧带(anterior cruciate ligament,ACL)断裂合并内侧半月板后角Ramp区损伤同期治疗的临床疗效,对ACL断裂合并内侧半月板Ramp区损伤患者的手术治疗提供一些参考。方法2018年3月至2020年8月佛山市第一人民医院关节骨外科收治23例ACL合并内侧Ramp区损伤患者,其中男15例,女8例;年龄19~64岁,平均(36.4±13.6)岁。接受关节镜下韧带重建同期行内侧半月板后角Ramp区修复手术,术后佩戴可调节膝关节角度的铰链支具3个月。术后3 d内行膝关节正侧位X线检查及CT骨隧道重建,末次随访时以前抽屉试验、Lachman试验评估膝关节稳定性及韧带松弛度,以2000国际膝关节评分委员会(international knee documentation committee,IKDC)膝关节评分、Lysholm膝关节评分和Tegner膝关节运动评分评估膝关节活动度及功能。结果23例均获随访,随访时间24~48个月,平均(34.0±8.0)个月。末次随访时ACL断裂合并Ramp区损伤患者2000 IKDC膝关节主观评分、Lysholm膝关节评分、Tegner膝关节运动评分分别从术前的(70.3±4.9)分、(58.0±7.0)分、(4.3±0.8)分提高到术后的(85.0±5.2)分、(81.6±6.1)分、(7.3±1.4)分,手术前后评分比较差异有统计学意义。23例患者患侧膝关节活动度平均(135.0±3.4)°,无活动受限。术后患者膝关节正侧位X线片示对线对位良好,未出现畸形,膝关节CT重建显示股骨及胫骨隧道位置良好,锚钉位置良好、固定牢固,MRI冠状面、矢状面、横断面序列未发现半月板后角Ⅲ级信号。23例患者随访期间均未发生韧带再损伤,未因半月板再次损伤而行关节镜手术。结论对ACL断裂合并内侧半月板Ramp区损伤患者,膝关节镜ACL重建同期行内侧半月板Ramp区修补术,术后膝关节稳定性、活动及功能明显改善,可进一步减少膝关节损伤,获得比较满意的活动度及关节功能。 展开更多
关键词 前交叉韧带损伤 半月板ramp区损伤 前交叉韧带重建 半月板修补 临床疗效
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Multi-Lever EarlyWarning forWind and Photovoltaic Power Ramp Events Based on Neural Network and Fuzzy Logic
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作者 Huan Ma Linlin Ma +3 位作者 Zengwei Wang Zhendong Li Yuanzhen Zhu Yutian Liu 《Energy Engineering》 EI 2024年第11期3133-3160,共28页
With the increasing penetration of renewable energy in power system,renewable energy power ramp events(REPREs),dominated by wind power and photovoltaic power,pose significant threats to the secure and stable operation... With the increasing penetration of renewable energy in power system,renewable energy power ramp events(REPREs),dominated by wind power and photovoltaic power,pose significant threats to the secure and stable operation of power systems.This paper presents an early warning method for REPREs based on long short-term memory(LSTM)network and fuzzy logic.First,the warning levels of REPREs are defined by assessing the control costs of various power control measures.Then,the next 4-h power support capability of external grid is estimated by a tie line power predictionmodel,which is constructed based on the LSTMnetwork.Finally,considering the risk attitudes of dispatchers,fuzzy rules are employed to address the boundary value attribution of the early warning interval,improving the rationality of power ramp event early warning.Simulation results demonstrate that the proposed method can generate reasonable early warning levels for REPREs,guiding decision-making for control strategy. 展开更多
关键词 Early warning machine learning power system security renewable energy power ramp event smart grid
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Ramping Up Reforms
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作者 Lan Xinzhen 《ChinAfrica》 2013年第12期38-39,共2页
THE Chinese Government released a range of reform and opening up policies, as is custom- ary. ahead of the Third Plenary Session of the 18th Central Committee of the Communist Party of China (CPC), which ran from No... THE Chinese Government released a range of reform and opening up policies, as is custom- ary. ahead of the Third Plenary Session of the 18th Central Committee of the Communist Party of China (CPC), which ran from November 9 to 12. This year. the corporate registration system was included in the reform package. 展开更多
关键词 ramping up Reforms
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Application of Connected Truck Data to Evaluate Spatiotemporal Impact of Rest Area Closures on Ramp Parking
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作者 Jijo K. Mathew Jairaj Desai +1 位作者 Edward D. Cox Darcy M. Bullock 《Journal of Transportation Technologies》 2024年第3期289-307,共19页
Ensuring adequate access to truck parking is critical to the safe and efficient movement of freight traffic. There are strict federal guidelines for commercial truck driver rest periods. Rest areas and private truck s... Ensuring adequate access to truck parking is critical to the safe and efficient movement of freight traffic. There are strict federal guidelines for commercial truck driver rest periods. Rest areas and private truck stops are the only places for the trucks to stop legally and safely. In locations without sufficient parking areas, trucks often park on interstate ramps, which create safety risks for other interstate motorists. Historically, agencies have employed costly and time intensive manual counting methods, camera surveillance, and driver surveys to assess truck parking. Connected truck data, available in near real-time, offers an efficient alternative to practitioners to assess truck parking patterns and identify areas where there may be insufficient safe parking spaces. This paper presents a case study of interstate I-70 in east central Indiana and documents the observed spatiotemporal impacts of a rest area closure on truck parking on nearby interstate ramps. Results showed that there was a 28% increase in parking on ramps during the rest area closure. Analysis also found that ramps closest to the rest area were most impacted by the closure, seeing a rise in truck parking sessions as high as 2.7 times. Parking duration on the ramps during rest area closure also increased drastically. Although it was expected that this would result in increased parking by trucks on adjacent ramps, this before, during, after scenario provided an ideal scenario to evaluate the robustness of these techniques to assess changing parking characteristics of long-haul commercial trucks. The data analytics and visualization tools presented in this study are scalable nationwide and will aid stakeholders in informed data-driven decision making when allocating resources towards improving the nations commercial vehicle parking infrastructure. 展开更多
关键词 Connected Truck Data Rest Areas Exit ramps Truck Parking Commercial Vehicles
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Design and Engineering of Urban Interchange Ramp Bridge Structure
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作者 Yuxiao Zhang Maode Yang 《Journal of Architectural Research and Development》 2024年第2期62-67,共6页
This paper analyzes the structural design of an urban interchange ramp bridge from four aspects,which are the superstructure,pier structure,foundation structure,and deck structure design to summarize the structural de... This paper analyzes the structural design of an urban interchange ramp bridge from four aspects,which are the superstructure,pier structure,foundation structure,and deck structure design to summarize the structural design ideas of this urban interchange ramp bridge,which can be used as a reference for future construction of the same bridge. 展开更多
关键词 Interchange ramp Bridge structure design Pier structure Foundation structure Bridge deck structure
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A Review of the Key Points in the Design of Small-Radius Curved Ramp Bridges
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作者 Yuxiao Zhang 《Journal of World Architecture》 2024年第1期97-102,共6页
This article explores the fundamentals of small-radius curved ramp bridges.It covers the selection of box girder spans,support methods,and forms,along with design optimization techniques for this type of bridge struct... This article explores the fundamentals of small-radius curved ramp bridges.It covers the selection of box girder spans,support methods,and forms,along with design optimization techniques for this type of bridge structure.The purpose of this paper is to provide robust support for enhancing the design quality of these bridges and ensuring their efficacy in real-world applications. 展开更多
关键词 Bridge design Small-radius curve ramp Box girder span Support method Support form
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利用RAMP和ISSR标记分析大麦种质资源的遗传多样性 被引量:23
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作者 侯永翠 颜泽洪 +2 位作者 兰秀锦 魏育明 郑有良 《中国农业科学》 CAS CSCD 北大核心 2005年第12期2555-2565,共11页
利用RAMP和ISSR两种标记分别对60份大麦种质资源遗传多样性进行了检测。结果发现,两种标记都能揭示材料间较高的遗传多样性,其中ISSR标记多态性又高于RAMP标记。在RAMP分析中,每个引物可扩增出1~10条DNA片段,平均为5.27条;22个RAMP引... 利用RAMP和ISSR两种标记分别对60份大麦种质资源遗传多样性进行了检测。结果发现,两种标记都能揭示材料间较高的遗传多样性,其中ISSR标记多态性又高于RAMP标记。在RAMP分析中,每个引物可扩增出1~10条DNA片段,平均为5.27条;22个RAMP引物共扩增出116条DNA片段,其中98条具有多态性,多态性比率(PPB)为84.48%;平均多态信息量(PIC)为0.277;每个位点有效等位基因数(Ne)为1.602;材料间遗传相似系数GS变化范围为0.551~0.965,平均值为0.830。在ISSR分析中,每个引物可获得1~10条DNA片段,平均为5.95条;19个ISSR引物共扩增出113条DNA片段,其中111条具有多态性,多态性比率(PPB)为98.23%;平均多态信息量(PIC)为0.427;每个位点有效等位基因数(Ne)为2.033;材料间遗传相似系数GS变幅为0.203~0.931,平均达0.676。聚类分析表明,两种标记都能将供试材料完全区分开,聚类结果具有一定的相似性,但也存在明显差异。Mantel检测表明,这两种标记极显著相关(r=0.346,t=2.808)。 展开更多
关键词 大麦 ramp ISSR 遗传多样性 遗传关系 聚类分析
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应用RAMP分子标记分析小豆栽培型种质资源遗传多样性 被引量:11
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作者 佘跃辉 荣廷昭 +2 位作者 粟生群 刘坚 田孟良 《作物学报》 CAS CSCD 北大核心 2006年第2期217-222,共6页
利用42对引物组合,对93份小豆栽培型种质资源的基因组DNA进行了RAMP分析,得到308条扩增谱带,其中297条(96.43%)具有多态性,每对引物组合可扩增出3-17条多态性谱带,平均7.1条,总遗传多样性指数或平均期望杂合度(Ht)达0.69... 利用42对引物组合,对93份小豆栽培型种质资源的基因组DNA进行了RAMP分析,得到308条扩增谱带,其中297条(96.43%)具有多态性,每对引物组合可扩增出3-17条多态性谱带,平均7.1条,总遗传多样性指数或平均期望杂合度(Ht)达0.693,表明小豆栽培型种质资源内存在较丰富的遗传多样性;小豆种质问遗传距离变异幅度为0.066-0.494,平均值为0.281,表明来源于不同生态区的小豆栽培型种质间的亲缘关系较近。利用RAMP扩增谱带数据进行聚类分析,可将93份小豆栽培型种质材料中的91份区分开,并划分为5个类群;其RAMP分子标记表现出明显的生育特性和生长习性趋同性,及一定的地域相关性。 展开更多
关键词 ramp 小豆 种质资源 遗传多样性
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应用RAMP分子标记研究红花资源遗传多样性 被引量:22
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作者 赵欢 吴卫 +2 位作者 郑有良 魏育明 杨玉霞 《植物遗传资源学报》 CAS CSCD 2007年第1期64-71,共8页
采用RAMP(Random amplified microsatellite polymorphism)对原产于42个国家的84份红花材料进行遗传多样性分析。结果表明,被测材料间RAMP标记多态性较高。16个引物组合所产生的122条DNA扩增片段中,有118条具有多态性,PPB为96.7%。PI... 采用RAMP(Random amplified microsatellite polymorphism)对原产于42个国家的84份红花材料进行遗传多样性分析。结果表明,被测材料间RAMP标记多态性较高。16个引物组合所产生的122条DNA扩增片段中,有118条具有多态性,PPB为96.7%。PIC的变化范围为0.580~0.978,平均值0.874。每个引物可扩增出4~11个DNA片段,平均获得7.6个DNA片断,其中7.4个具有多态性,遗传相似系数(GS)的变化范围0.338~0.907,平均值为0.665。基于GS的聚类结果可以将所有84份材料完全分开,并划分为6类,聚类结果与材料的地理分布有一定关系,来源于亚洲和美洲的材料多样性相对比较丰富,所有来自中国的材料被聚为一大类。据此认为,红花种质资源在分子水平上确实存在较大遗传差异,RAMP分子标记是评价红花资源遗传多样性的有效方法。 展开更多
关键词 红花 ramp标记 遗传多样性 聚类分析
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应用RAMP分子标记探讨仲彬草属的种间关系 被引量:19
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作者 张利 周永红 +2 位作者 魏育明 郑有良 刘世贵 《高技术通讯》 EI CAS CSCD 2003年第4期28-33,共6页
对仲彬草属Kengyilia 14个种和1个变种进行了RAMP分析。结果表明物种间遗传差异明显。40个引物组合产生的254条DNA扩增片段中,216条(85.0%)具有多态性,每个引物组合可扩增出1~9条多态性带,平均5.4条。遗传相似系数变化范围为0.327~0.... 对仲彬草属Kengyilia 14个种和1个变种进行了RAMP分析。结果表明物种间遗传差异明显。40个引物组合产生的254条DNA扩增片段中,216条(85.0%)具有多态性,每个引物组合可扩增出1~9条多态性带,平均5.4条。遗传相似系数变化范围为0.327~0.886,平均值为0.549。同时,形态相似、地理分布一致的物种有一定的亲缘关系,聚类在一起。这与RAPD、形态学和细胞学等分析结果基本一致。因此,RAMP分子标记是评价仲彬草属种间关系十分有效的方法。 展开更多
关键词 ramp分子标记 仲彬草属 种间关系 形态学 细胞学 遗传变异 亲缘关系
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基于RAMP密度-刚度插值格式的结构拓扑优化 被引量:16
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作者 罗震 陈立平 +1 位作者 黄玉盈 张云清 《计算力学学报》 EI CAS CSCD 北大核心 2005年第5期585-591,共7页
基于RAM P密度-刚度插值格式和优化准则(OC)方法,提出一种改进的拓扑优化设计的数学模型及设计变量的迭代格式。对棋盘格式和网格依赖性等数值计算问题进行了综述和研究,针对敏度过滤技术中存在的“边缘扩散”现象,提出了一种混合的过... 基于RAM P密度-刚度插值格式和优化准则(OC)方法,提出一种改进的拓扑优化设计的数学模型及设计变量的迭代格式。对棋盘格式和网格依赖性等数值计算问题进行了综述和研究,针对敏度过滤技术中存在的“边缘扩散”现象,提出了一种混合的过滤技术。给出了拓扑优化设计的程序流程,用FORTRAN程序实现了算法,通过几个经典算例证明了本文所研究方法的有效性。 展开更多
关键词 拓扑优化 ramp插值模型 优化准则法 混合过滤法
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80份甘蔗种质RAMP标记遗传多样性分析 被引量:15
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作者 王英 陈守俊 +1 位作者 朱相成 庄南生 《植物遗传资源学报》 CAS CSCD 北大核心 2011年第4期525-532,共8页
采用RAMP分子标记技术对80份甘蔗种质(32份祖亲种、48份栽培品种或品系)的遗传基础进行了分析。从30对引物组合中筛选出4对多态性较强引物,构建了甘蔗80份种质的RAMP指纹图谱,这4对引物组合共扩增出84条带,其多态性为91.7%。80份种质... 采用RAMP分子标记技术对80份甘蔗种质(32份祖亲种、48份栽培品种或品系)的遗传基础进行了分析。从30对引物组合中筛选出4对多态性较强引物,构建了甘蔗80份种质的RAMP指纹图谱,这4对引物组合共扩增出84条带,其多态性为91.7%。80份种质的遗传相似系数变化范围在0.433~0.988,平均0.710。聚类分析表明,随着相似系数结合线的不同,可分别将参试的甘蔗种质从属间(甘蔗属与斑茅种)、野生种(割手密种、大茎野生种、印度种、中国种)与栽培种(热带种)间、栽培种与杂交栽培品种(或品系)间区别开来。各祖亲种与杂交栽培品种(或品系)的遗传相似性由大到小依次为热带种〉印度种和中国种〉大茎野生种〉云南割手密种〉其他割手密种〉斑茅。另外,本试验首次利用RAMP标记,获得了部分热带种、野生种及斑茅种特异片段,并发现这些特异片段能不同程度地在具有其血缘的栽培种中得到传递。 展开更多
关键词 甘蔗 指纹图谱 ramp 相似系数 特异片段
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“福星01”人参与黄果人参及人参农家类型的ISSR、RAMP分析 被引量:7
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作者 王志清 王英平 +3 位作者 郭靖 赵亚会 李昌禹 田丽 《吉林农业大学学报》 CAS CSCD 北大核心 2010年第3期293-298,306,共7页
利用RAMP和ISSR2种标记分别对人参品种及农家类型间的遗传差异进行检测和分析。结果表明:2种标记均能揭示各材料间的遗传多样性,其中RAMP标记的多态性高于ISSR标记。在ISSR标记中,每个引物可扩增出1~7条DNA片段,24个引物扩增出97条带,... 利用RAMP和ISSR2种标记分别对人参品种及农家类型间的遗传差异进行检测和分析。结果表明:2种标记均能揭示各材料间的遗传多样性,其中RAMP标记的多态性高于ISSR标记。在ISSR标记中,每个引物可扩增出1~7条DNA片段,24个引物扩增出97条带,平均为4.04条。其中45条带具有多态性,多态性比率为46.4%,揭示供试材料间遗传相似系数(GS)为0.7526~0.9691,平均值为0.8304。在RAMP分析中,每条引物可扩增出1~8条DNA片段,23对引物共扩增出112条带,平均为4.87条。其中75条带具有多态性,多态性比率66.9%,揭示供试材料间遗传相似系数(GS)为0.5625~0.9286,平均值为0.7114。聚类分析结果表明,2种标记均能将供试材料完全分开,分类具有一定的相似性,即聚类与地域性有一定关系。 展开更多
关键词 人参品种 农家类型 ISSR ramp 遗传关系
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萝卜基因组DNA的RAMP-PCR体系优化 被引量:7
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作者 龚义勤 李培 +2 位作者 王明霞 赵丽萍 柳李旺 《植物研究》 CAS CSCD 北大核心 2006年第1期93-97,共5页
以萝卜为材料,对基因组DNA的RAMP分析体系中的Mg^2+、dNTPs和引物浓度进行优化。分别设计3个浓度梯度:Mg^2+为0.75、1.5、3.0mmol·L^-1;dNTPs为0.05、0.15、0.3mmol·L^-1;引物为0.065、0.2、0.4μmol·L^-... 以萝卜为材料,对基因组DNA的RAMP分析体系中的Mg^2+、dNTPs和引物浓度进行优化。分别设计3个浓度梯度:Mg^2+为0.75、1.5、3.0mmol·L^-1;dNTPs为0.05、0.15、0.3mmol·L^-1;引物为0.065、0.2、0.4μmol·L^-1,并对合适退火温度进行研究。筛选出的RAMP优化体系为(20μL):dNTPs 0.15mmol·L^-1,Mg^2+ 1.5mmol·L^-1,引物0.2~0.4μmol·L^-1,DNA 10ng,Taq E 0.8U;PCR扩增程序为94℃ 3min,94℃ 1min,45℃ 1min,72℃ 1.5min,42个循环,72℃ 8min。运用此体系,进行引物组合筛选,并对7个萝卜品种的遗传多样性与品种鉴定进行RAMP标记分析。 展开更多
关键词 萝卜 ramp标记 体系优化 遗传多样性
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