Cryo-ET Workflow for Understanding Ion Channels Localization on the Nodes of Ranvier

The localization of ion channels on myelinated axon is closely related with the saltatory conduction of action potential (AP). Abnormal changes in these channels contribute to multiple mental diseases. The development of cryo-Electron Tomography (cryo-ET) has provided a promising prospect for peering into ion channels in their native environment at high resolution. Previous achievements are reviewed here on cryo-ET. Accordingly, a cryo-ET workflow is designed for understanding ion channels localization in myelinated axon, especially nodes of Ranvier, which are significant for the saltatory conduction involved in the propagation of high-speed AP. The workflow is divided into six parts: the preparation of neural cultures with myelin, antibodies and immunofluorescence staining, frozen-hydrated sample preparation, cryo-ET imaging, cryo-correlative light and electron microscopy (cryo-CLEM) imaging, three-dimensional (3D) reconstruction and refinement. The purpose is to conceive a possible solution for the problems related to ion channel compounds including localization, conformation dynamics, accessory structures of ion channel and transient regulatory factors, and thus provide insights into treating neurological diseases caused by abnormal ion channels activity.

Reassembly of the axon initial segment and nodes of Ranvier in regenerated axons of the central nervous system

Myelinated axons of the peripheral and central nervous system（PNS＆CNS）are divided into molecularly distinct excitable domains,including the axon initial segment（AIS）and nodes of Ranvier.The AIS is composed of a dense network of cytoskeletal proteins,cell adhesion molecules,and voltage gated ion channels and is located at the proximal most region of the axon（Koleand Stuart, 2012）.

经皮二氧化碳分压监测在硬质支气管镜下Feng′s EBUS-TBNB中的应用

目的:探讨经皮二氧化碳分压(transcutaneous partial pressure of carbon dioxide,PtcCO_(2))监测在硬质支气管镜下冯氏气管内超声引导纵隔切开活检(Feng′s endobronchial ultrasound-guided transbronchial node biopsy,Feng′s EBUS-TBNB)中的应用。方法:收集2019年8月—2022年8月期间天津医科大学总医院收治的纵隔疾病患者128例,随机分成Feng′s EBUS-TBNB组和支气管内超声引导针吸活检术(endobronchial ultrasound-guided transbronchial needle aspiration,EBUS-TBNA)组,患者均予高频喷射通气行硬质支气管镜下操作及PtcCO_(2)监测,EBUS-TBNA组患者行EBUS-TBNA,Feng′s EBUS-TBNB组患者行Feng′s EBUS-TBNB术,比较两组患者术前(T0)、通气开始时(T_(1))、通气10 min后(T_(2))、通气20 min后(T3)、通气30 min后(T_(4))、通气40 min后(T_(5))、通气50 min后(T_(6))、通气60 min后(T_(7))心率(heart rate,HR)、平均动脉压(mean arterial pressure,MAP)和PtcCO_(2)的变化。结果:T_(1)~T_(7)时,两组患者HR、MAP和PtcCO_(2)水平均明显高于T0时(P<0.05)。T0~T_(2)时,两组患者HR、MAP和PtcCO_(2)水平比较差异无统计学意义(P>0.05);T3~T_(5)时,Feng′s EBUS-TBNB组HR、MAP和PtcCO_(2)水平均明显高于EBUS-TBNA组(P<0.05);而T_(6)~T_(7)时,两组患者HR、MAP和PtcCO_(2)水平比较差异无统计学意义(P>0.05)。结论:将PtcCO_(2)应用于Feng′s EBUS-TBNB中,可间接反映患者HR、MAP水平变化,值得临床推广应用。

Direct contacts of microglia on myelin sheath and Ranvier’s node in the corpus callosum in rats

Over the recent years, it has been found that microglia pseudopodia contact synapses, detect sick ones and prune them, even in adult animals. Myelinated nerves also carry out plasticity in which microglia remove myelin debris by phagocytosis. However, it remains unknown whether microglia explore structures on nerve fibers, such as Ranvier’s node(RN) or myelin sheath, before they become debris. By double or triple staining RNs or myelin sheathes and microglia in healthy rat corpus callosum, this study unveiled direct contacts of microglia pseudopodia with RNs and with para-and inter-nodal myelin sheathes, which was then verified by electron microscopic observations. Our data indicated that microglia also explore unmyelinated nerve fibers. Furthermore, we used the animals with matured white matter;therefore, microglia may be actively involved in plasticity of matured white matter tracts as it does for synapse pruning, instead of only passively phagocytize myelin debris.

A New Layer W/Cu/S Polymer with Heterobimetallic Nodes and Tridentate Trithiocyanuric Acid

Solvothermal reaction of [WS4]2-with CuCN and trithiocyanuric acid（L1） in orga-nic solvents gave rise to a new W/Cu/S polymer with a 2D anionic network,namely [Et4N]2[WS4Cu3（C3N3S3H1.5）2]（1）.The anionic layer of 1 is constructed by the T-shaped {WS4Cu3} subunits as nodes and L1 as linkers and features a（4,4） topology.Both 1 and its isomorphous compound [Et4N]2[MoS4Cu3（C3N3S3H1.5）2]（2） have been fully characterized by X-ray crystal analysis,IR and microanalyses.Compounds 1 and 2 show optical transitions with band gaps of 2.17 and 1.84 eV,respectively.

Visualizing Complex Anatomical Structure in Bamboo Nodes Based on X-ray Microtomography

In recent years,bamboo has been widely used in a broad range of applications,a thorough understanding of the structural characteristics of bamboo nodes is essential for better processing and manufacturing of biomimetic materials.This study investigated the complex anatomical structure for the nodes of two bamboo species,Indocalamus latifolius(Keng)McClure and Shibataea chinensis Nakai,using a high-resolution X-ray microtomography(μCT).The results show that the vascular bundle system in the nodal region of I.latifolius and S.chinensis is a net-like structure composed of horizontal and axial vascular bundles.Furthermore,the fiber sheath surrounding metaxylem vessels tended to be shorter in the tangential direction.This structure of bamboo nodes facilitates the tangential and axial transport of moisture and nutrients.The anatomical structure of I.latifolius and S.chinensis nodes has obvious differences,especially in the arrangement of vascular bundles.Vascular bundle frequency was significantly higher in S.chinensis nodes than in I.latifolius nodes.These findings indicate thatμCT is a nondestructive three-dimensional imaging method that can used to examine the anatomical structure of bamboo nodes.

Evidential method to identify influential nodes in complex networks

Identifying influential nodes in complex networks is still an open issue. In this paper, a new comprehensive centrality mea- sure is proposed based on the Dempster-Shafer evidence theory. The existing measures of degree centrality, betweenness centra- lity and closeness centrality are taken into consideration in the proposed method. Numerical examples are used to illustrate the effectiveness of the proposed method.

A rare presentation of unicentric Castleman's disease in the thigh:A case report and review of literature

BACKGROUND Castleman's disease(CD)is a rare lymphoproliferative,emulating both benign and malignant diseases.The diagnosis of CD is formulated upon the combination of clinical and laboratory criteria and ultimately confirmed by histopathological assessment.Due to its rarity,CD presents a challenge in treatment selection,with available options encompassing surgery,chemotherapy,and autologous stem cell transplantation.However,studies suggest that surgical resection of the lesion is the most effective treatment modality,especially for unicentric CD(UCD).CASE SUMMARY Here,we describe the case of a 25-year-old woman who presented with painless left thigh swelling for 10 wk.She had been following a low-fat diet to lose weight and had normal laboratory results.Magnetic resonance imaging revealed a wellcircumscribed,demarcated cystic lesion located in the left inguinal region with eccentrically positioned signal void vascular structures,measuring 4.3 cm×3 cm×3.2 cm,likely of lymphoid origin.The patient underwent surgical resection,and the final histopathology showed a vascular proliferation and hyalinization of the vessel walls,along with atretic germinal centers traversed by penetrating vessels,consistent with CD.The patient was discharged home one day after the procedure in good condition,with a follow-up appointment scheduled in our outpatient clinic.CONCLUSION Although surgical resection is the mainstay for UCD,a multidisciplinary approach is needed due the lack of specific diagnostic features and treatments.

Extracting Multiple Nodes in a Brain Region of Interest for Brain Functional Network Estimation and Classification

Purpose: Brain functional networks (BFNs) has become important approach for diagnosis of some neurological or psychological disorders. Before estimating BFN, obtaining blood oxygen level dependent (BOLD) representative signals from brain regions of interest (ROIs) is important. In the past decades, the common method is generally to take a ROI as a node, averaging all the voxel time series inside it to extract a representative signal. However, one node does not represent the entire information of this ROI, and averaging method often leads to signal cancellation and information loss. Inspired by this, we propose a novel model extraction method based on an assumption that a ROI can be represented by multiple nodes. Methods: In this paper, we first extract multiple nodes (the number is user-defined) from the ROI based on two traditional methods, including principal component analysis (PCA), and K-means (Clustering according to the spatial position of voxels). Then, canonical correlation analysis (CCA) was issued to construct BFNs by maximizing the correlation between the representative signals corresponding to the nodes in any two ROIs. Finally, to further verify the effectiveness of the proposed method, the estimated BFNs are applied to identify subjects with autism spectrum disorder (ASD) and mild cognitive impairment (MCI) from health controls (HCs). Results: Experimental results on two benchmark databases demonstrate that the proposed method outperforms the baseline method in the sense of classification performance. Conclusions: We propose a novel method for obtaining nodes of ROId based on the hypothesis that a ROI can be represented by multiple nodes, that is, to extract the node signals of ROIs with K-means or PCA. Then, CCA is used to construct BFNs.

基于s域节点导纳矩阵的谐振稳定性分析方法

谐振稳定性问题在新型电力系统背景下日益突出,目前缺乏在大系统层面进行谐振稳定性分析的有效方法。在阐述谐振稳定性的定义和机理的基础上,提出了基于s域节点导纳矩阵原理实现谐振稳定性分析的完整方法。该方法分2个阶段完成,首先在假定系统阻尼为0的条件下确定各谐振模态的无阻尼谐振频率、节点电压振型和最佳可控可观测节点;然后在考虑系统所有阻尼的条件下,基于系统降阶和系统辨识的思路,采用测试信号法精确计算出谐振模态对应的特征根。进一步还可计算出各谐振模态对特定元件参数的灵敏度。该方法避免了直接求解s域节点导纳矩阵行列式零点的困难,为在大系统层面开展电力系统谐振稳定性分析提供了可靠途径。

S100A4 over-expression underlies lymph node metastasis and poor prognosis in colorectal cancer

AIM:To develop lymph node metastasis(LNM)-associated biomarkers for colorectal cancer(CRC) using quantitative proteome analysis.METHODS:Differences in protein expression between primary CRC with LNM(LNM CRC) and without LNM(non-LNM CRC) were assessed using methyl esterification stable isotope labeling coupled with 2D liquid chromatography followed by tandem mass spectrometry(2D-LC-MS/MS).The relationship to clinicopathological parameters and prognosis of candidate biomarkers was examined using an independent sample set.RESULTS:Forty-three proteins were found to be differentially expressed by at least 2.5-fold in two types of CRC.S100A4 was significantly upregulated in LNM CRC compared with non-LNM CRC,which was confirmed by Western blotting,immunohistochemistry and real-time quantitative polymerase chain reaction.Further immunohistochemistry on another 112 CRC cases showed that overexpression of S100A4 frequently existed in LNM CRC compared with non-LNM CRC(P < 0.001).Overexpression of S100A4 was significantly associated with LNM(P < 0.001),advanced TNM stage(P < 0.001),increased 5-year recurrence rate(P < 0.001) and decreased 5-year overall survival rate(P < 0.001).Univariate and multivariate analyses indicated that S100A4 expression was an independent prognostic factor for recurrence and survival of CRC patients(P < 0.05).CONCLUSION:S100A4 might serve as a powerful biomarker for LNM and a prognostic factor in CRC.

基于node.js和Qt的B/S+C/S混合模式下的专用业务信息系统研究与设计

随着互联网技术的发展及各式商用信息产品的普及,专用业务信息系统用户的需求和使用习惯的变化更加频繁,传统C/S架构的专用业务信息系统不能很好地满足新的市场变化的需求。针对这一现状,本文使用HTML5、node.js、Qt Web Engine、Thrift等一系列新的技术,并吸取B/S架构的优势,提出了一种B/S+C/S混合模式系统架构,采用了服务化和分布式的思想,使得系统既具备了C/S模式强大的数据操作和事务处理能力,又兼容了B/S模式便捷的维护升级以及良好的用户体验特性,能够对用户的新需求进行快速响应。

基于Node.js的网上书店设计与实现

在互联网迅猛发展的今天,以其为背景的电子商务正呈井喷式发展,人们的购物习惯随之改变。利用网络来满足人们日常购物需求的形式,逐渐变为人们主体的购物形式。该文系统采用B/S结构,使用Node.js搭建服务器端运行环境,并配合Vue框架实现前端代码以及MySQL管理后台数据库。网上书店的角色分为用户和管理员2种,主要实现了用户注册、登录、按商品分类查看书籍、按商品名称进行搜索、查看商品详情、商品的下单以及订单管理等功能。

基于Node.js技术的综合测评系统的设计与实现

该文采用Node.js＋Mongodb＋Bootstrap开发技术,设计并实现一个B/S结构的大学生综合测评系统,实现了综合测评、测评信息查询与管理、测评规则设置与管理、活动管理、用户管理及系统配置等功能。

基于Node.js的家庭智能地暖远程监控系统

智能家居系统中往往会有远程控制部分,这些远程监控系统通常是基于单一C/S或B/S模式实现的,其功能和性能受到单一模式的限制,无法最大化满足用户需求。本文以家庭智能地暖控制系统为项目背景,基于现在流行的Node.js设计了一个C/S和B/S混合模式的远程监控系统,重点描述基于Node.js构架服务器的方法,并介绍其中网页与客户端部分的设计工具与方案。

Cervical Castleman's disease mimicking lymph node metastasis of esophageal carcinoma

Castleman's disease(CD) is an uncommon benign lymphoproliferative disorder of unknown etiology. A rare case of cervical CD diagnosed at lymph node dissection for esophageal carcinoma is reported. An esophageal tumor was identified in a 67-year-old man during a follow-up examination after surgery for oral carcinoma. Esophagoscopy revealed a type 1 tumor in the cervical esophagus. Histology of esophagoscopic biopsies indicated squamous cell carcinoma. Contrastenhanced computed tomography revealed swollen lymph nodes of the right cervical region. No distant metastasis was detected. Esophageal carcinoma, T2N2M0, Stage ⅢA was diagnosed. Neoadjuvant chemotherapy was recommended, but the patient rejected the chemotherapy. The patient underwent laparoscopic-assisted transhiatal esophagectomy. The histopathological diagnosis was moderately differentiated squamous cell carcinoma with pT1bN0M0, Stage ⅠA. On histology, the swollen lymph nodes of the right cervical region revealed CD. The patient's postoperative course was relatively good.

Isolated synchronous Virchow lymph node metastasis of sigmoid cancer: A case report

BACKGROUND Colorectal cancer(CRC)is one of the most common malignant tumors of the digestive tract.Lymphatic metastases of this tumor are mostly confined to the regional lymph nodes,and distant supraclavicular lymph node metastases are very rare.CASE SUMMARY In this report,we describe a patient with sigmoid carcinoma and isolated synchronous supraclavicular lymph node metastases.A 56-year-old male presented with a left cervical mass that was confirmed as a lymph node metastasis from sigmoid cancer by several auxiliary examinations.After 6 cycles of chemotherapy with the 5-fluorouracil,leucovorin and oxaliplatin+cetuximab regimen,the sigmoid colon tumor and Virchow’s lymph node metastasis were significantly smaller than before treatment,and no new metastatic sites were observed.Considering the effects of chemotherapy on quality of life,resection of the primary tumor was performed followed by 4 cycles of chemotherapy with the original chemotherapy regimen.Virchow’s lymph node dissection was selected by mutual consultation between the patient and us.After the second surgery,the patient received capecitabine and cetuximab chemotherapy and did not experience recurrence or metastasis during follow-up.CONCLUSION In conclusion,supraclavicular lymph node metastasis without any other solid organ metastasis is a potential metastatic pathway for CRC.In addition,after resection of the primary lesion,postoperative chemotherapy combined with supraclavicular lymph node dissection is feasible for the treatment of patients with CRC and isolated synchronous Virchow’s lymph node metastases.

Effect of Altitude, Right Ascension of Ascending Node and Inclination on Lifetime of Circular Lunar Orbits

The lifetime of a lunar satellite orbit is constrained by the non-spherical nature of the Moon’s gravity field. The orbital lifetime of lunar orbits depends significantly on the initial conditions of the orbit. Right ascension of ascending node (Ω) is one of the important orbital parameter affecting the orbital lifetime. In the present work we have analyzed the effect of Ω on the variation of lifetime with altitude for circular lunar orbits. It is found that at a particular initial altitude, a small increase in the altitude results in substantial increase in the orbital lifetime due to effect of the long periodic terms of Earth’s gravity on eccentricity and this transition altitude is different for different Ω. Further, it is observed that the variation of transition altitude with Ω follows a definite, but different trend for orbits with different inclinations. The transition altitude for polar orbits is found to be higher without the effect of Sun and Earth gravity. Variation of transition altitude with orbital inclination is also analyzed. Lifetimes of high altitude circular lunar orbits are analyzed and it is observed that at high altitudes lifetime decreases with altitude.

基于C++的ANSYS RST文件中节点力数据读取方法

通过C++程序以及ANSYS提供的应用编程接口,给出了一种提取ANSYSRST结果文件中节点力数据的方法。使用ANSYS对某地铁车辆焊接构架的有限元模型进行静力学求解,获得包含节点力数据的RST文件,并将该方法读取的节点力数据与疲劳寿命计算软件WeldFatigue的计算结果进行对比,验证了利用该方法提取数据的准确性与适用性。

Geographic Drone-based Route Optimization Approach for Emergency Area Ad-Hoc Network

Wireless sensor Mobile ad hoc networks have excellent potential in moving and monitoring disaster area networks on real-time basis.The recent challenges faced in Mobile Ad Hoc Networks(MANETs)include scalability,localization,heterogeneous network,self-organization,and self-sufficient operation.In this background,the current study focuses on specially-designed communication link establishment for high connection stability of wireless mobile sensor networks,especially in disaster area network.Existing protocols focus on location-dependent communications and use networks based on typically-used Internet Protocol(IP)architecture.However,IP-based communications have a few limitations such as inefficient bandwidth utilization,high processing,less transfer speeds,and excessive memory intake.To overcome these challenges,the number of neighbors(Node Density)is minimized and high Mobility Nodes(Node Speed)are avoided.The proposed Geographic Drone Based Route Optimization(GDRO)method reduces the entire overhead to a considerable level in an efficient manner and significantly improves the overall performance by identifying the disaster region.This drone communicates with anchor node periodically and shares the information to it so as to introduce a drone-based disaster network in an area.Geographic routing is a promising approach to enhance the routing efficiency in MANET.This algorithm helps in reaching the anchor(target)node with the help of Geographical Graph-Based Mapping(GGM).Global Positioning System(GPS)is enabled on mobile network of the anchor node which regularly broadcasts its location information that helps in finding the location.In first step,the node searches for local and remote anticipated Expected Transmission Count(ETX),thereby calculating the estimated distance.Received Signal Strength Indicator(RSSI)results are stored in the local memory of the node.Then,the node calculates the least remote anticipated ETX,Link Loss Rate,and information to the new location.Freeway Heuristic algorithm improves the data speed,efficiency and determines the path and optimization problem.In comparison with other models,the proposed method yielded an efficient communication,increased the throughput,and reduced the end-to-end delay,energy consumption and packet loss performance in disaster area networks.