Strigolactones(SLs)are newly discovered plant hormones which regulate the normal development of different plant organs,especially root architecture.Lateral root formation of rapeseed seedlings before winter has great ...Strigolactones(SLs)are newly discovered plant hormones which regulate the normal development of different plant organs,especially root architecture.Lateral root formation of rapeseed seedlings before winter has great effects on the plant growth and seed yield.Here,we treated the seedlings of Zhongshuang 11(ZS11),an elite conventional rapeseed cultivar,with different concentrations of GR24(a synthetic analogue of strigolactones),and found that a low concentration(0.18μmol L–1)of GR24 could significantly increase the lateral root growth,shoot growth,and root/shoot ratio of seedlings.RNA-Seq analysis of lateral roots at 12 h,1 d,4 d,and 7 d after GR24 treatment showed that 2301,4626,1595,and 783 genes were significantly differentially expressed,respectively.Function enrichment analysis revealed that the plant hormone transduction pathway,tryptophan metabolism,and the phenylpropanoid biosynthesis pathway were over-represented.Moreover,transcription factors,including AP2/ERF,AUX/IAA,NAC,MYB,and WRKY,were up-regulated at 1 d after GR24 treatment.Metabolomics profiling further demonstrated that the amounts of various metabolites,such as indole-3-acetic acid(IAA)and cis-zeatin were drastically altered.In particular,the concentrations of endogenous IAA significantly decreased by 52.4 and 75.8%at 12 h and 1 d after GR24 treatment,respectively.Our study indicated that low concentrations of exogenous SLs could promote the lateral root growth of rapeseed through interaction with other phytohormones,which provides useful clues for the effects of SLs on root architecture and crop productivity.展开更多
Melatonin is a naturally occurring compound in plants. Here, we tested the effect of exogenous melatonin on rapeseed(Brassica napus L.) grown under salt stress. Application of 30 μmol L^-1 melatonin alleviated salt...Melatonin is a naturally occurring compound in plants. Here, we tested the effect of exogenous melatonin on rapeseed(Brassica napus L.) grown under salt stress. Application of 30 μmol L^-1 melatonin alleviated salt-induced growth inhibition, and the shoot fresh weight, the shoot dry weight, the root fresh weight, and the root dry weight of seedlings treated with exogenous melatonin increased by 128.2, 142.9, 122.2, and 124.2%, respectively, compared to those under salt stress. In addition, several physiological parameters were evaluated. The activities of antioxidant enzymes including peroxidase(POD), catalase(CAT) and ascorbate peroxidase(APX) were enhanced by 16.5, 19.3, and 14.2% compared to their activities in plants without exogenous melatonin application under salt stress, while the H2O2 content was decreased by 11.2% by exogenous melatonin. Furthermore, melatonin treatment promoted solute accumulation by increasing the contents of proline(26.8%), soluble sugars(15.1%) and proteins(58.8%). The results also suggested that higher concentrations(〉50 μmol L^-1) of melatonin could attenuate or even prevent the beneficial effects on seedling development. In conclusion, application of a low concentration of exogenous melatonin to rapeseed plants under salt stress can improve the H2O2-scavenging capacity by enhancing the activities of antioxidant enzymes such as POD, CAT and APX, and can also alleviate osmotic stress by promoting the accumulation of osmoregulatory substances such as soluble proteins, proline, and water soluble glucan. Ultimately, exogenous melatonin facilitates root development and improves the biomass of rapeseed seedlings grown under salt stress, thereby effectively alleviating the damage of salt stress in rapeseed seedlings.展开更多
The investigation was carried out to study the response of winter rapeseed to potassium (K) feritlization and the critical soil available K level for current winter rapeseed production in the Yangtze River Valley (...The investigation was carried out to study the response of winter rapeseed to potassium (K) feritlization and the critical soil available K level for current winter rapeseed production in the Yangtze River Valley (YRV) of China. A total of 132 field experiments were conducted in fields of farmers in the major winter rapeseed growing areas in YRV in 2000/2001 and 2004/2005 to 2006/2007 during growing season. Results of these field experiments showed that the average rapeseed yield increment resulting from 100 kg K ha-1 application was 358 kg ha-1, an increase over the control CK (no K) of 18.0% in 2005/2006 and 2006/2007. The average internal use efficiency (IE) of K was higher in the CK treatment (21.9 kg grain, kg-1 K uptake) than in the +K (100 kg K ha-1) treatment (17.7 kg grain, kg-1 K uptake). Winter rapeseed required 68.1 kg of K to produce 1 000 kg seed. The recovery efficiency of K fertilizer in rapeseed production averaged 39.3%. The K balance was negative, with an average net removal of 117.6 kg K ha-1 in the CK treatment annually, and 56.8 kg K ha-1 in the +K treatment. The results indicated that there was a significant negative relationship between yield increments by K application and soil available K content. Based on the relative yield of CK/+K at 90% level, the critical level of soil available K (NH4OAc-extractable K) was 135 mg kg-1.展开更多
14 yellow-seeded rapeseed lines (Brassia napus L.) from different genetic sources were used to analyze diversity of testa pigments content, oil and protein content, and RAPD markers. The results showed that the anthoc...14 yellow-seeded rapeseed lines (Brassia napus L.) from different genetic sources were used to analyze diversity of testa pigments content, oil and protein content, and RAPD markers. The results showed that the anthocyanin and melanin were the most important pigments in testa and their content were responsible for the variation in seed color ranging from orange to black yellow, 14 yellow-seeded lines could be classified into 3 groups: high anthocyanin content group with anthocyanin content over 2. 54 mg g-1 DW, the seed color was light yellow or orange; low pigments content group with low content of anthocyanin and melanin, the testa was transparent and the seed color was light yellow, greenish yellow or twany; high melanin content group with melanin content over 178. 4U(A290nm) , the testa was black, the seed color was black yellow. Oil content changed from 36.2% to 45. 5%, protein content from 21.1% to 27.7% , and the correlation analysis revealed that the oil content is highly significantly negatively correlated with the protein content. The cluster analysis showed that the extensive genetic variation existed among 14 yellow-seeded lines by using unweighted paired group method with arithmetic average (UPGMA) based on RAPD markers which were amplified with decamer primers, the genetic similarity among them ranged from 0. 25 to 0.909, and 14 yellow-seeded lines could put into 2 clusters corresponding to genome difference.展开更多
Glucosinolates(GSLs) and their hydrolytic products contribute to the quality traits of rapeseed flowering stalk tissues, such as taste, flavor and anticarcinogenic properties(Glucoraphanin). However, little is known a...Glucosinolates(GSLs) and their hydrolytic products contribute to the quality traits of rapeseed flowering stalk tissues, such as taste, flavor and anticarcinogenic properties(Glucoraphanin). However, little is known about the genetic mechanisms of GSL accumulation in rapeseed flowering stalks. In this study, the variation and genetic architecture of GSL metabolites in flowering stalk tissues were investigated for the first time among a panel of 107 accessions. All GSL compounds exhibited continuous and wide variations in the present population. Progoitrin,glucobrassicanapin and gluconapin were the most abundant GSL compounds. Five quantitative trait loci(QTL) significantly associated with three GSL compounds were identified by genome-wide association study. GRA_C04 was under selected during modern breeding, in which the ratio of lower GSL haplotype(HAP2) in the accessions bred before 1990(52.56%) was significantly lower than that after 1990(78.95%). Four candidate genes, BnaA01. SOT16, BnaA06. SOT17, Bna A06. MYB51a, and Bna A06. MYB51b, were identified in the GTL_A01 and 4OH_A06 regions.These findings provide new insights into GSL biosynthesis in flowering stalk tissues and facilitate quality improvement in rapeseed flowering stalks.展开更多
Nutrient deficiency stresses often occur simultaneously in soil. Thus, it's necessary to investigate the mechanisms underlying plant responses to multiple stresses through identification of some key stress-responsive...Nutrient deficiency stresses often occur simultaneously in soil. Thus, it's necessary to investigate the mechanisms underlying plant responses to multiple stresses through identification of some key stress-responsive genes. Quantitative real-time PCR (qRT-PCR) is essential for detecting the expression of the interested genes, of which the selection of suitable reference genes is a crucial step before qRT-PCR. To date, reliable reference genes to normalize qRT-PCR data under different nutrient deficiencies have not been reported in plants. In this study, expression of ten candidate reference genes was detected in leaves and roots of rapeseed (Brassica napus L.) after implementing different nutrient deficiencies for 14 days. These candidate genes, included two traditionally used reference genes and eight genes selected from an RNA- Seq dataset. Two software packages (GeNorm, NormFinder) were employed to evaluate candidate gene stability. Results showed that VHA-E1 was the highest-ranked gene in leaves of nutrient-deficient rapeseed, while VHA-G1 and UBC21 were most stable in nutrient-deficient roots. When rapeseed leaves and roots were combined, UBC21, HTB1, VHA-G1 and A CT7 were most stable among all samples. To evaluate the stabilities of the highest-ranked genes, the relative expression of two target genes, BnTrxl;1 and BnPhtl;3 Were further determined. The results showed that the relative expression of BnTrxl;1 depended on reference gene selection, suggesting that it's necessary to evaluate the stability of reference gene prior to qRT-PCR. This study provides suitable reference genes for gene expression analysis of rapeseed responses to different nutrient deficiencies, which is essential for elucidation of mechanisms underlying rapeseed responses to multiple nutrient deficiency stresses展开更多
To better understand waterlogging effect on leaf senescence in winter rapseed (Brassica napus L.) during flowering stage, experiments were designed to explore foliar K application influences on adverse effects of wate...To better understand waterlogging effect on leaf senescence in winter rapseed (Brassica napus L.) during flowering stage, experiments were designed to explore foliar K application influences on adverse effects of waterlogging stress. Winter rapeseed was sprayed with K after waterlogging at initial flowering stage. Results indicated that waterlogging significantly decreased leaf net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci) and transpiration rate (Tr). It also declined maximum quantum yield of PS II (Fv/Fm), quantum yield of electron transport (ΦPS II) and photochemical quenching (qP), but increased leaf non-photochemical quenching (NPQ) and minimal fluorescence (Fo). Interestingly, exogenous application of K significantly alleviated waterlogging-induced photosynthesis inhibition. Foliar K application increased RuBisCO activation, chlorophyll and soluble protein contents, while significantly decreased MDA content under waterlogging stress. Moreover, K supplementation improved accumulation of K+, Ca2+, Mg2+, N, Zn2+, Mn2+, Fe2+ in leaves. In general, foliar K application is effective inalleviating deleterious effects of waterlogging stress and delays leaf senescence of winter rapeseed.展开更多
As a big rapeseed(Brassica napus L.) producing and consuming country, China provides more and more rapeseed oil year by year. With the development of demand for rapeseed and import of edible oil, rapeseed breeding and...As a big rapeseed(Brassica napus L.) producing and consuming country, China provides more and more rapeseed oil year by year. With the development of demand for rapeseed and import of edible oil, rapeseed breeding and production is facing a great challenge. New situation has proposed higher requirements to yield, oil quality and safety, etc. In the review, some aspects about rapeseed breeding in China were reported, including breeding of high-oil content rapeseed, breeding of extremely early-maturing rapeseed, development of mechanization and development of chemical emasculation. Some prospects and expectations were also proposed.展开更多
Rapeseed(Brassica napus L.) is the world?s second produced oilseed and accounts for nearly 12% of world major vegetable oil production.For the last 10 years,the production,planting area,and yield of rapeseed have been...Rapeseed(Brassica napus L.) is the world?s second produced oilseed and accounts for nearly 12% of world major vegetable oil production.For the last 10 years,the production,planting area,and yield of rapeseed have been stable,with improvement of seed quality and especially seed oil content.This paper presented the worldwide rapeseed research by using the bibliometric analysis of papers published during the period 2011–2021.In total,7617 articles and reviews were included in this analysis.Our results showed that the global publication on rapeseed increased substantially in the past 11 years,and the number of publications of China grew significantly in particular.China was the largest contributor to rapeseed research with higher H-index value and number of citations compared to the other countries.China also had the largest number of leading universities and institutions,of which Huazhong Agricultural University was the most productive.The scientific mapping including co-authorship network of countries/regions and authors,and co-occurrence network of author keywords were analyzed using VOSviewer software.The most common rapeseed research topics included oil,rapeseed meal,yield,biodiesel,fatty acids,salt stress,and proteins,while the current research focused on topics such as:abiotic stress,evolution,expression analysis,phylogenetic analysis,heterosis,polyploidy,and transcriptomics.This paper depicts the knowledge structure of current global rapeseed research that may help direct the future studies for relevant researchers.展开更多
In the last decade,some disease occurred on our experimental farms that had caused serious losses.They were not caused by fungi,bacteria or viruses.By loop-mediated isothermal amplification(LAMP)technique,the detectio...In the last decade,some disease occurred on our experimental farms that had caused serious losses.They were not caused by fungi,bacteria or viruses.By loop-mediated isothermal amplification(LAMP)technique,the detection results pointed to the possible pathogen as phytoplasma.The investigation results implied that phytoplasmas could cause more than 13 kinds of symptoms in almost all parts of plants in B.napus L.,including witches’broom,multi-stems,aggregate main inflorescences,and flat stems.The incidences of these phytoplasma-associated diseases in our experimental farms rose from 1.61%in 2010 to 6.00%in 2021.Some phytoplasma infected plants died without any growing points.These studies would be helpful for detecting phytoplasmas diseases,selecting disease resistant germplasm and improving varieties with disease resistances in B.napus L.展开更多
RNA sequencing of the sensitive GH01 variety of Brassica napus L. seedling roots under 12 h of waterlogging was compared with previously published data of the ZS9 tolerant variety to unravel genetic mechanisms of wate...RNA sequencing of the sensitive GH01 variety of Brassica napus L. seedling roots under 12 h of waterlogging was compared with previously published data of the ZS9 tolerant variety to unravel genetic mechanisms of waterlogging tolerance beyond natural variation. A total of 2 977 genes with similar expression patterns and 17 genes with opposite expression patterns were identiifed in the transcription proifles of ZS9 and GH01. An additional 1 438 genes in ZS9 and 1 861 genes in GH01 showed strain speciifc regulation. Analysis of the overlapped genes between ZS9 and GH01 revealed that waterlogging tolerance is determined by ability to regulate genes with similar expression patterns. Moreover, differences in both gene expression proifles and abscisic acid (ABA) contents between the two varieties suggest that ABA may play some role in waterlogging tolerance. This study identiifes a subset of candidate genes for further functional analysis.展开更多
基金Funds were provided by the National Key Research and Development Program of China (2018YFD1000900)
文摘Strigolactones(SLs)are newly discovered plant hormones which regulate the normal development of different plant organs,especially root architecture.Lateral root formation of rapeseed seedlings before winter has great effects on the plant growth and seed yield.Here,we treated the seedlings of Zhongshuang 11(ZS11),an elite conventional rapeseed cultivar,with different concentrations of GR24(a synthetic analogue of strigolactones),and found that a low concentration(0.18μmol L–1)of GR24 could significantly increase the lateral root growth,shoot growth,and root/shoot ratio of seedlings.RNA-Seq analysis of lateral roots at 12 h,1 d,4 d,and 7 d after GR24 treatment showed that 2301,4626,1595,and 783 genes were significantly differentially expressed,respectively.Function enrichment analysis revealed that the plant hormone transduction pathway,tryptophan metabolism,and the phenylpropanoid biosynthesis pathway were over-represented.Moreover,transcription factors,including AP2/ERF,AUX/IAA,NAC,MYB,and WRKY,were up-regulated at 1 d after GR24 treatment.Metabolomics profiling further demonstrated that the amounts of various metabolites,such as indole-3-acetic acid(IAA)and cis-zeatin were drastically altered.In particular,the concentrations of endogenous IAA significantly decreased by 52.4 and 75.8%at 12 h and 1 d after GR24 treatment,respectively.Our study indicated that low concentrations of exogenous SLs could promote the lateral root growth of rapeseed through interaction with other phytohormones,which provides useful clues for the effects of SLs on root architecture and crop productivity.
基金supported by the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (CAAS)the Hubei Agricultural Science and Technology Innovation Center, Chinathe Canola Key Industrial Innovation Team of Xiaogan, China
文摘Melatonin is a naturally occurring compound in plants. Here, we tested the effect of exogenous melatonin on rapeseed(Brassica napus L.) grown under salt stress. Application of 30 μmol L^-1 melatonin alleviated salt-induced growth inhibition, and the shoot fresh weight, the shoot dry weight, the root fresh weight, and the root dry weight of seedlings treated with exogenous melatonin increased by 128.2, 142.9, 122.2, and 124.2%, respectively, compared to those under salt stress. In addition, several physiological parameters were evaluated. The activities of antioxidant enzymes including peroxidase(POD), catalase(CAT) and ascorbate peroxidase(APX) were enhanced by 16.5, 19.3, and 14.2% compared to their activities in plants without exogenous melatonin application under salt stress, while the H2O2 content was decreased by 11.2% by exogenous melatonin. Furthermore, melatonin treatment promoted solute accumulation by increasing the contents of proline(26.8%), soluble sugars(15.1%) and proteins(58.8%). The results also suggested that higher concentrations(〉50 μmol L^-1) of melatonin could attenuate or even prevent the beneficial effects on seedling development. In conclusion, application of a low concentration of exogenous melatonin to rapeseed plants under salt stress can improve the H2O2-scavenging capacity by enhancing the activities of antioxidant enzymes such as POD, CAT and APX, and can also alleviate osmotic stress by promoting the accumulation of osmoregulatory substances such as soluble proteins, proline, and water soluble glucan. Ultimately, exogenous melatonin facilitates root development and improves the biomass of rapeseed seedlings grown under salt stress, thereby effectively alleviating the damage of salt stress in rapeseed seedlings.
基金funded by the National Key Technologies R&D Program during the 11th Five-Year Plan period of China (2008BADA4B08)the Program for New Century Excellent Talents in University of Ministry of Education of China (NCET-07-0345)+2 种基金the Earmarked Fund for Modern Agro-Industry Technology Research System of Ministry of Agriculture of China (nycytx-005)the collaborative project between China and the International Plant Nutrition Institute (IPNI)between China and the International Potash Institute (IPI)
文摘The investigation was carried out to study the response of winter rapeseed to potassium (K) feritlization and the critical soil available K level for current winter rapeseed production in the Yangtze River Valley (YRV) of China. A total of 132 field experiments were conducted in fields of farmers in the major winter rapeseed growing areas in YRV in 2000/2001 and 2004/2005 to 2006/2007 during growing season. Results of these field experiments showed that the average rapeseed yield increment resulting from 100 kg K ha-1 application was 358 kg ha-1, an increase over the control CK (no K) of 18.0% in 2005/2006 and 2006/2007. The average internal use efficiency (IE) of K was higher in the CK treatment (21.9 kg grain, kg-1 K uptake) than in the +K (100 kg K ha-1) treatment (17.7 kg grain, kg-1 K uptake). Winter rapeseed required 68.1 kg of K to produce 1 000 kg seed. The recovery efficiency of K fertilizer in rapeseed production averaged 39.3%. The K balance was negative, with an average net removal of 117.6 kg K ha-1 in the CK treatment annually, and 56.8 kg K ha-1 in the +K treatment. The results indicated that there was a significant negative relationship between yield increments by K application and soil available K content. Based on the relative yield of CK/+K at 90% level, the critical level of soil available K (NH4OAc-extractable K) was 135 mg kg-1.
文摘14 yellow-seeded rapeseed lines (Brassia napus L.) from different genetic sources were used to analyze diversity of testa pigments content, oil and protein content, and RAPD markers. The results showed that the anthocyanin and melanin were the most important pigments in testa and their content were responsible for the variation in seed color ranging from orange to black yellow, 14 yellow-seeded lines could be classified into 3 groups: high anthocyanin content group with anthocyanin content over 2. 54 mg g-1 DW, the seed color was light yellow or orange; low pigments content group with low content of anthocyanin and melanin, the testa was transparent and the seed color was light yellow, greenish yellow or twany; high melanin content group with melanin content over 178. 4U(A290nm) , the testa was black, the seed color was black yellow. Oil content changed from 36.2% to 45. 5%, protein content from 21.1% to 27.7% , and the correlation analysis revealed that the oil content is highly significantly negatively correlated with the protein content. The cluster analysis showed that the extensive genetic variation existed among 14 yellow-seeded lines by using unweighted paired group method with arithmetic average (UPGMA) based on RAPD markers which were amplified with decamer primers, the genetic similarity among them ranged from 0. 25 to 0.909, and 14 yellow-seeded lines could put into 2 clusters corresponding to genome difference.
基金supported by the Key Research and Development project of Hubei Province (Grant Nos. 2020BBB083, 2021BBA097 and 2021BBA102)the National Key Research and Development Program of China (Grant No. 2016YFD0100202)。
文摘Glucosinolates(GSLs) and their hydrolytic products contribute to the quality traits of rapeseed flowering stalk tissues, such as taste, flavor and anticarcinogenic properties(Glucoraphanin). However, little is known about the genetic mechanisms of GSL accumulation in rapeseed flowering stalks. In this study, the variation and genetic architecture of GSL metabolites in flowering stalk tissues were investigated for the first time among a panel of 107 accessions. All GSL compounds exhibited continuous and wide variations in the present population. Progoitrin,glucobrassicanapin and gluconapin were the most abundant GSL compounds. Five quantitative trait loci(QTL) significantly associated with three GSL compounds were identified by genome-wide association study. GRA_C04 was under selected during modern breeding, in which the ratio of lower GSL haplotype(HAP2) in the accessions bred before 1990(52.56%) was significantly lower than that after 1990(78.95%). Four candidate genes, BnaA01. SOT16, BnaA06. SOT17, Bna A06. MYB51a, and Bna A06. MYB51b, were identified in the GTL_A01 and 4OH_A06 regions.These findings provide new insights into GSL biosynthesis in flowering stalk tissues and facilitate quality improvement in rapeseed flowering stalks.
基金supported by the grants from the Agricultural Science and Technology Innovation Program,Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2013OCRI)the Excellent Young Scientist Fund of Chinese Academy of Agricultural Sciences(1610172015004)an open project funded by State Key Laboratory for the Conservation and Utilization of Subtropical Agro-bioresources,China(SKLCUSA-b201403)
文摘Nutrient deficiency stresses often occur simultaneously in soil. Thus, it's necessary to investigate the mechanisms underlying plant responses to multiple stresses through identification of some key stress-responsive genes. Quantitative real-time PCR (qRT-PCR) is essential for detecting the expression of the interested genes, of which the selection of suitable reference genes is a crucial step before qRT-PCR. To date, reliable reference genes to normalize qRT-PCR data under different nutrient deficiencies have not been reported in plants. In this study, expression of ten candidate reference genes was detected in leaves and roots of rapeseed (Brassica napus L.) after implementing different nutrient deficiencies for 14 days. These candidate genes, included two traditionally used reference genes and eight genes selected from an RNA- Seq dataset. Two software packages (GeNorm, NormFinder) were employed to evaluate candidate gene stability. Results showed that VHA-E1 was the highest-ranked gene in leaves of nutrient-deficient rapeseed, while VHA-G1 and UBC21 were most stable in nutrient-deficient roots. When rapeseed leaves and roots were combined, UBC21, HTB1, VHA-G1 and A CT7 were most stable among all samples. To evaluate the stabilities of the highest-ranked genes, the relative expression of two target genes, BnTrxl;1 and BnPhtl;3 Were further determined. The results showed that the relative expression of BnTrxl;1 depended on reference gene selection, suggesting that it's necessary to evaluate the stability of reference gene prior to qRT-PCR. This study provides suitable reference genes for gene expression analysis of rapeseed responses to different nutrient deficiencies, which is essential for elucidation of mechanisms underlying rapeseed responses to multiple nutrient deficiency stresses
文摘To better understand waterlogging effect on leaf senescence in winter rapseed (Brassica napus L.) during flowering stage, experiments were designed to explore foliar K application influences on adverse effects of waterlogging stress. Winter rapeseed was sprayed with K after waterlogging at initial flowering stage. Results indicated that waterlogging significantly decreased leaf net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci) and transpiration rate (Tr). It also declined maximum quantum yield of PS II (Fv/Fm), quantum yield of electron transport (ΦPS II) and photochemical quenching (qP), but increased leaf non-photochemical quenching (NPQ) and minimal fluorescence (Fo). Interestingly, exogenous application of K significantly alleviated waterlogging-induced photosynthesis inhibition. Foliar K application increased RuBisCO activation, chlorophyll and soluble protein contents, while significantly decreased MDA content under waterlogging stress. Moreover, K supplementation improved accumulation of K+, Ca2+, Mg2+, N, Zn2+, Mn2+, Fe2+ in leaves. In general, foliar K application is effective inalleviating deleterious effects of waterlogging stress and delays leaf senescence of winter rapeseed.
基金Supported by Earmarked Fund for Modern Agro-industry Technology Research System of China(CARS-13)National Key Research and Development Plan(JFYS2016ZY03002156)+6 种基金Ministry of Agriculture Experimental Observation of the Upper Reaches of the Yangtze River Oil Crop Science Station(09203020)Sichuan Crop Breeding CommunityInnovation Ability Promotion Project of Sichuan Provincial Finance(2016zypz-013)Sichuan Province Innovation Team FundingNatural Science Foundation of China(NSFC31360262)Science and Technology Achievements Transformation Project of Sichuan Province(2017CC0052)SAAS Modern Agricultural Technology Special Fund(2015JSCX-012)
文摘As a big rapeseed(Brassica napus L.) producing and consuming country, China provides more and more rapeseed oil year by year. With the development of demand for rapeseed and import of edible oil, rapeseed breeding and production is facing a great challenge. New situation has proposed higher requirements to yield, oil quality and safety, etc. In the review, some aspects about rapeseed breeding in China were reported, including breeding of high-oil content rapeseed, breeding of extremely early-maturing rapeseed, development of mechanization and development of chemical emasculation. Some prospects and expectations were also proposed.
文摘Rapeseed(Brassica napus L.) is the world?s second produced oilseed and accounts for nearly 12% of world major vegetable oil production.For the last 10 years,the production,planting area,and yield of rapeseed have been stable,with improvement of seed quality and especially seed oil content.This paper presented the worldwide rapeseed research by using the bibliometric analysis of papers published during the period 2011–2021.In total,7617 articles and reviews were included in this analysis.Our results showed that the global publication on rapeseed increased substantially in the past 11 years,and the number of publications of China grew significantly in particular.China was the largest contributor to rapeseed research with higher H-index value and number of citations compared to the other countries.China also had the largest number of leading universities and institutions,of which Huazhong Agricultural University was the most productive.The scientific mapping including co-authorship network of countries/regions and authors,and co-occurrence network of author keywords were analyzed using VOSviewer software.The most common rapeseed research topics included oil,rapeseed meal,yield,biodiesel,fatty acids,salt stress,and proteins,while the current research focused on topics such as:abiotic stress,evolution,expression analysis,phylogenetic analysis,heterosis,polyploidy,and transcriptomics.This paper depicts the knowledge structure of current global rapeseed research that may help direct the future studies for relevant researchers.
基金the financial support provided by project of Science and Technology Tackling Key Problems in Henan Province(Grant No.182102110430)the National Key Research and Development Program of China(Grant No.2016YFD0101300)by China Agricultural Research system(CARS-12).
文摘In the last decade,some disease occurred on our experimental farms that had caused serious losses.They were not caused by fungi,bacteria or viruses.By loop-mediated isothermal amplification(LAMP)technique,the detection results pointed to the possible pathogen as phytoplasma.The investigation results implied that phytoplasmas could cause more than 13 kinds of symptoms in almost all parts of plants in B.napus L.,including witches’broom,multi-stems,aggregate main inflorescences,and flat stems.The incidences of these phytoplasma-associated diseases in our experimental farms rose from 1.61%in 2010 to 6.00%in 2021.Some phytoplasma infected plants died without any growing points.These studies would be helpful for detecting phytoplasmas diseases,selecting disease resistant germplasm and improving varieties with disease resistances in B.napus L.
文摘RNA sequencing of the sensitive GH01 variety of Brassica napus L. seedling roots under 12 h of waterlogging was compared with previously published data of the ZS9 tolerant variety to unravel genetic mechanisms of waterlogging tolerance beyond natural variation. A total of 2 977 genes with similar expression patterns and 17 genes with opposite expression patterns were identiifed in the transcription proifles of ZS9 and GH01. An additional 1 438 genes in ZS9 and 1 861 genes in GH01 showed strain speciifc regulation. Analysis of the overlapped genes between ZS9 and GH01 revealed that waterlogging tolerance is determined by ability to regulate genes with similar expression patterns. Moreover, differences in both gene expression proifles and abscisic acid (ABA) contents between the two varieties suggest that ABA may play some role in waterlogging tolerance. This study identiifes a subset of candidate genes for further functional analysis.