Estrone has been identified as a potential endocrine-disrupting chemical （EDC）[1]. Estrone is usually quantified by gas chromatography-mass spectrometry （GC-MS）, GC-MS/MS, high performance liquid chromatography （...Estrone has been identified as a potential endocrine-disrupting chemical （EDC）[1]. Estrone is usually quantified by gas chromatography-mass spectrometry （GC-MS）, GC-MS/MS, high performance liquid chromatography （HPLC）, HPLC- MS, and HPLC-MS/MS, etc.[2-3]. Meanwhile, several immunoassays based on radioimmunoassay, enzyme linked immunosorbent assay （ELISA） or chemiluminescence immunoassay （CLIA） for determination of estrone in real samples have been developed[2＇4]. Although these methods are sensitive, they need multistage separation and are thus time-consuming and laborious. A very promising way for the simplification of immunoassays for routine applications is a shift from heterogeneous methods （with separation） to homogeneous assays （without separation）[5]. Fluorescence polarization immunoassay （FPIA） is one of the homogeneous techniques that meets the requirements of a simple, reliable, fast, and cost-effective analysis[6]. Therefore, the present study is focused on the development of FPIA in order to analyze estrone based on antibody production.展开更多
The aims of this study are to use quantitative analysis of the prostate-specific antigen(PSA)in the seminal stain examination and to explore the practical value of this analysis in forensic science.For a comprehensive...The aims of this study are to use quantitative analysis of the prostate-specific antigen(PSA)in the seminal stain examination and to explore the practical value of this analysis in forensic science.For a comprehensive analysis,vaginal swabs from 48 rape cases were tested both by a PSA fluorescence analyzer(i-CHROMA Reader)and by a conventional PSA strip test.To confirm the results of these PSA tests,seminal DNA was tested following differential extraction.Compared to the PSA strip test,the PSA rapid quantitative fluorescence analyzer provided the more accurate and sensitive results.More importantly,individualized schemes based on quantitative PSA results of samples can be developed to improve the quality and procedural efficiency in the forensic seminal inspection of samples prior to DNA analysis.展开更多
In January 2019,the fourth rabies case caused by organ transplantation was noticed in China,with the conditions of one per year for the recent four years.Different from the previous cases,there were no definite epidem...In January 2019,the fourth rabies case caused by organ transplantation was noticed in China,with the conditions of one per year for the recent four years.Different from the previous cases,there were no definite epidemiological histories of exposure or rabies-related symptoms from this patient.This case strongly supports the call for the legislation of establishing a national-level management that will incorporate the screening programs on donors prior to the practice of organ transplantation to reduce the risks on rabies caused by organ transplantation.展开更多
基金supported by Natural Science Foundation of China(U1301214)Guangdong Natural Science Foundation(S2013030013338)+1 种基金the PhD Programs Foundation of Ministry of Education of China(20114404130002)National Department Public Benefit Research Foundation(201003008-08)
文摘Estrone has been identified as a potential endocrine-disrupting chemical （EDC）[1]. Estrone is usually quantified by gas chromatography-mass spectrometry （GC-MS）, GC-MS/MS, high performance liquid chromatography （HPLC）, HPLC- MS, and HPLC-MS/MS, etc.[2-3]. Meanwhile, several immunoassays based on radioimmunoassay, enzyme linked immunosorbent assay （ELISA） or chemiluminescence immunoassay （CLIA） for determination of estrone in real samples have been developed[2＇4]. Although these methods are sensitive, they need multistage separation and are thus time-consuming and laborious. A very promising way for the simplification of immunoassays for routine applications is a shift from heterogeneous methods （with separation） to homogeneous assays （without separation）[5]. Fluorescence polarization immunoassay （FPIA） is one of the homogeneous techniques that meets the requirements of a simple, reliable, fast, and cost-effective analysis[6]. Therefore, the present study is focused on the development of FPIA in order to analyze estrone based on antibody production.
基金support from the Program for Young Innovative Research Team in China University of Political Science and Law.
文摘The aims of this study are to use quantitative analysis of the prostate-specific antigen(PSA)in the seminal stain examination and to explore the practical value of this analysis in forensic science.For a comprehensive analysis,vaginal swabs from 48 rape cases were tested both by a PSA fluorescence analyzer(i-CHROMA Reader)and by a conventional PSA strip test.To confirm the results of these PSA tests,seminal DNA was tested following differential extraction.Compared to the PSA strip test,the PSA rapid quantitative fluorescence analyzer provided the more accurate and sensitive results.More importantly,individualized schemes based on quantitative PSA results of samples can be developed to improve the quality and procedural efficiency in the forensic seminal inspection of samples prior to DNA analysis.
基金supported by the Key Technologies R&D Programof the National Ministry of Science(2017YFC1200503).
文摘In January 2019,the fourth rabies case caused by organ transplantation was noticed in China,with the conditions of one per year for the recent four years.Different from the previous cases,there were no definite epidemiological histories of exposure or rabies-related symptoms from this patient.This case strongly supports the call for the legislation of establishing a national-level management that will incorporate the screening programs on donors prior to the practice of organ transplantation to reduce the risks on rabies caused by organ transplantation.
