为了评估法医学DNA数据库建设中涉及的59个Y-STR基因座的遗传多态性和法医学应用效能,通过AGCU Y SUPP PLUS试剂盒和AGCU Y37试剂盒检测374个广东汉族无关男性个体,将检测出的59个Y-STR基因座按照突变率的高低进行分类组合和统计分析。...为了评估法医学DNA数据库建设中涉及的59个Y-STR基因座的遗传多态性和法医学应用效能,通过AGCU Y SUPP PLUS试剂盒和AGCU Y37试剂盒检测374个广东汉族无关男性个体,将检测出的59个Y-STR基因座按照突变率的高低进行分类组合和统计分析。结果显示,59个Y-STR基因座联合运用在374个无关男性个体中检出了374个单倍型,其中44个中低突变Y-STR组合、15个高快突变Y-STR组合分别检出373和372个单倍型。59个Y-STR的基因多态性数值分布在0.0551(DYS645)~0.9580(DYF387S1 a/b)之间。结果表明,这59个Y-STR在广东汉族群体中均具有良好的多态性,按中低突变Y-STR组合和高快突变Y-STR组合研发新的检测体系可更好地满足法医实践的不同需求。展开更多
为提升小白链霉菌(Streptomyces albluus)产ε-聚赖氨酸(ε-poly-L-lysine,ε-PL)能力,在常压室温等离子体(atmospheric and room temperature plasma,ARTP)、硫酸二乙酯(diethyl sulfate,DES)单因子诱变的基础上,采用ARTP-DES连续诱变...为提升小白链霉菌(Streptomyces albluus)产ε-聚赖氨酸(ε-poly-L-lysine,ε-PL)能力,在常压室温等离子体(atmospheric and room temperature plasma,ARTP)、硫酸二乙酯(diethyl sulfate,DES)单因子诱变的基础上,采用ARTP-DES连续诱变。连续诱变条件:ARTP工作功率110 W,照射距离2 mm,照射时间为30、60、105 s;DES诱变体积分数0.6%,反应时间20 min。并改进一种快速筛选方法,结合链霉素抗性、亚甲基蓝透明圈及48微孔板培养,酶标仪快速测定ε-PL含量。结果表明:ARTP-DES连续诱变结合链霉素抗性正突变率可达40.8%,同时,获得1株遗传性能稳定的链霉素抗性突变株S. albulus AD-9,其ε-PL摇瓶产量为2.1 g/L,是出发菌株的2.1倍。通过研究高产菌株的生理特性,发现其在营养需求、发酵过程中菌球形态等都发生了变化。ARTP-DES连续诱变选育高产ε-PL菌株是一种高效选育手段。展开更多
Objective: We report the results of Y-chromosomal profile and mtDNA (mitochondrial DNA) of the Chevalier Bayard (1476?-1524). Methods: His genomic DNA was extracted from a tooth of his mandible. His Y-STRs profile was...Objective: We report the results of Y-chromosomal profile and mtDNA (mitochondrial DNA) of the Chevalier Bayard (1476?-1524). Methods: His genomic DNA was extracted from a tooth of his mandible. His Y-STRs profile was obtained using the AmFirst identifier PCR amplification kit. The mtDNA genomic sequence intervals for HVR1 and HVR2 were amplified by PCR, with specific primers. Results: We obtained the complete STR (Short Tandem Repeats) profile, based on fourteen STRs (DYS19, DYS385.a, DYS389.I and .b, DYS390, DYS391, DYS392, DYS393, DYS438, DYS439, DYS448, DYS456 and DYS458 and Y-GATA-H4). The deduced Y-STRs profile corresponds to the sub-clade S21 of the major European haplogroup R1b-M269 (the “Germanic” haplotype). There are six mutations (16093C, 16211T and 16519C in the HVR1 sequence, 263G, 309.1C and 315.1C in the HVR2 sequence) in the mtDNA of Bayard. The 263G mutation determines the H mtDNA haplogroup and the 16211T suggests the H5 sub-clade of the H haplogroup (a sub-clade found at >8% frequency in France, at the periphery of the Alpine arch region). This sub-clade H5 (subsequently assimilated to the H10e haplotype) is that (with a perfect match) of a modern living male related (to 32 generations) to the Bayard maternal ascendance. The Bayard mtDNA haplotype was found once only in a database of 100 South-German mtDNA control sequences. Conclusions: The resulting R1b-M269 Y haplogroup established confirms the Germanic origin of the Bayard ancestors, suggested by genealogic studies concerning his paternal ascendance. The result concerning the mtDNA H10e haplotype found in the modern living male related to Bayard by matrilinear ascendance establishes that the DNA tooth is well of him, with a 99% of chance.展开更多
从KOWCH and EMANUEL(2015)的结论“热带气旋的快速增强过程并无特殊物理机制在起作用”出发,利用JTWC西北太平洋TC最佳路径数据集(best track)和3种不同积云对流参数化方案模拟的数据资料对西北太平洋上热带气旋的强度变化和路径偏折...从KOWCH and EMANUEL(2015)的结论“热带气旋的快速增强过程并无特殊物理机制在起作用”出发,利用JTWC西北太平洋TC最佳路径数据集(best track)和3种不同积云对流参数化方案模拟的数据资料对西北太平洋上热带气旋的强度变化和路径偏折进行研究分析,进行重新验证,得出以下结论:①决定TC的迅速增强过程和非快速增强过程的物理机制是有差异的;②肥尾效应在TC路径突变中比TC在迅速增强中更明显;③不同对流参数化方案对TC的强度变化模拟不同,其中KFMT方案模拟效果最好,KF方案次之,SAS方案最差。但无论是KF、KFMT还是SAS方案,对TC强度迅速增强的模拟能力都很弱,在减弱阶段均能较好模拟;④3种方案对TC路径偏折的模拟都较好,两端皆有较明显的肥尾现象,表明TC的路径突变确实是有特殊物理机制在起作用。展开更多
To evaluate the promising advantages of massively parallel sequencing(MPS)in our casework,we analysed a total of 33 Y-chromosomal short tandem repeats(Y-STRs)with traditional capillary electrophoresis(CE)and 25 Y-STRs...To evaluate the promising advantages of massively parallel sequencing(MPS)in our casework,we analysed a total of 33 Y-chromosomal short tandem repeats(Y-STRs)with traditional capillary electrophoresis(CE)and 25 Y-STRs using the newer MPS technology.We studied the outcome of both technologies in 64 father-son pairs using stock and custom-designed kits.Current MPS technology confirmed the 13 mutational events observed with CE and improved our understanding of the complex nature of STR mutations.By detecting isometric sequence variants between unrelated males,we show that sequencing Y-STRs using MPS can boost discrimination power.展开更多
Short tandem repeats on the Y chromosome(Y-STRs),characterized by paternal inheritance,are valuable in forensic practice.Notably,the potential application of Y-STRs in pedigrees should be drawn upon,especially in Chin...Short tandem repeats on the Y chromosome(Y-STRs),characterized by paternal inheritance,are valuable in forensic practice.Notably,the potential application of Y-STRs in pedigrees should be drawn upon,especially in China’s surname-concentrated natural villages.The study focused on 50 Y-STRs,including 13 rapidly mutating(RM)Y-STRs that largely constitute the current Y-STR commercial kits,and determined the differences in these Y-STRs between branches in a large pedigree and the discriminatory power of these haplotypes in different units for male relatives.As indicated in the results,14 inconsistencies were observed at 9 Y-STRs between 10 father-son pairs.In addition,these 50 Y-STR haplotypes discriminated 10 out of 47 father-son pairs,106 of 148 cousin pairs,70 of 119 uncle-nephew pairs,17 of 39 brother pairs,and 14 out of 33 grandfather-grandson pairs in a large pedigree.The RM Y-STR set is able to differentiate close male relatives in a large pedigree.展开更多
目的评估线性探针方法快速检测结核分枝杆菌的耐药性效果。方法选取2013年-2014年江西省耐药监测点分离培养的阳性菌株,收集菌体,提取DNA,采用MTBDRplus试剂盒进行检测,以国际标准的比例法检测结果作为对照,评价其敏感度及特异度,并分...目的评估线性探针方法快速检测结核分枝杆菌的耐药性效果。方法选取2013年-2014年江西省耐药监测点分离培养的阳性菌株,收集菌体,提取DNA,采用MTBDRplus试剂盒进行检测,以国际标准的比例法检测结果作为对照,评价其敏感度及特异度,并分析突变分布情况。结果检测利福平耐药的灵敏度为93.33%,特异度为98.35%;检测异烟肼的灵敏度为76.31%,特异度为98.83%。耐利福平基因突变频率最高的位点为rpo B H531L(57.89%),耐异烟肼基因突变频率最高的位点是kat G S315T1(60.61%)。结论可利用MTBDRplus快速检测利福平和异烟肼的耐药性,是快速发现耐药结核分枝杆菌的有效方法。展开更多
文摘目的探讨应用x TAG70plex液相芯片技术对非小细胞肺癌(NSCLC)患者的快速现场评价(ROSE)细胞学制片进行EGFR、KRAS和PIK3CA基因检测的可行性及其临床价值。方法纳入就诊于天津医科大学总医院行支气管镜检查并最终病理诊断为NSCLC的患者75例ROSE细胞学制片和配对的组织学标本,分别用x TAG70plex液相芯片进行EGFR、KRAS和PIK3CA基因突变检测。结果除1例在组织学标本检出为KRAS突变而在配对的ROSE细胞学标本没有检测出来的病例,其他病例基因突变检测结果均相同,两种标本EGFR基因突变检测结果一致率为100%,KRAS基因突变检测结果一致率为98.7%。结论 x TAG70plex液相芯片技术可以有效地检测ROSE细胞学制片EGFR、KRAS和PIK3CA的基因突变状态,对于NSCLC患者,ROSE细胞学制片可以作为组织学替代标本进行基因检测。
文摘Objective: We report the results of Y-chromosomal profile and mtDNA (mitochondrial DNA) of the Chevalier Bayard (1476?-1524). Methods: His genomic DNA was extracted from a tooth of his mandible. His Y-STRs profile was obtained using the AmFirst identifier PCR amplification kit. The mtDNA genomic sequence intervals for HVR1 and HVR2 were amplified by PCR, with specific primers. Results: We obtained the complete STR (Short Tandem Repeats) profile, based on fourteen STRs (DYS19, DYS385.a, DYS389.I and .b, DYS390, DYS391, DYS392, DYS393, DYS438, DYS439, DYS448, DYS456 and DYS458 and Y-GATA-H4). The deduced Y-STRs profile corresponds to the sub-clade S21 of the major European haplogroup R1b-M269 (the “Germanic” haplotype). There are six mutations (16093C, 16211T and 16519C in the HVR1 sequence, 263G, 309.1C and 315.1C in the HVR2 sequence) in the mtDNA of Bayard. The 263G mutation determines the H mtDNA haplogroup and the 16211T suggests the H5 sub-clade of the H haplogroup (a sub-clade found at >8% frequency in France, at the periphery of the Alpine arch region). This sub-clade H5 (subsequently assimilated to the H10e haplotype) is that (with a perfect match) of a modern living male related (to 32 generations) to the Bayard maternal ascendance. The Bayard mtDNA haplotype was found once only in a database of 100 South-German mtDNA control sequences. Conclusions: The resulting R1b-M269 Y haplogroup established confirms the Germanic origin of the Bayard ancestors, suggested by genealogic studies concerning his paternal ascendance. The result concerning the mtDNA H10e haplotype found in the modern living male related to Bayard by matrilinear ascendance establishes that the DNA tooth is well of him, with a 99% of chance.
文摘从KOWCH and EMANUEL(2015)的结论“热带气旋的快速增强过程并无特殊物理机制在起作用”出发,利用JTWC西北太平洋TC最佳路径数据集(best track)和3种不同积云对流参数化方案模拟的数据资料对西北太平洋上热带气旋的强度变化和路径偏折进行研究分析,进行重新验证,得出以下结论:①决定TC的迅速增强过程和非快速增强过程的物理机制是有差异的;②肥尾效应在TC路径突变中比TC在迅速增强中更明显;③不同对流参数化方案对TC的强度变化模拟不同,其中KFMT方案模拟效果最好,KF方案次之,SAS方案最差。但无论是KF、KFMT还是SAS方案,对TC强度迅速增强的模拟能力都很弱,在减弱阶段均能较好模拟;④3种方案对TC路径偏折的模拟都较好,两端皆有较明显的肥尾现象,表明TC的路径突变确实是有特殊物理机制在起作用。
基金supported by the Internal Security Funding Police Program of the European Commission-Directorate General MigrationHome Affairs under the European Commission[grant number HOME/2014/ISFP/AG/LAWX/4000007135].
文摘To evaluate the promising advantages of massively parallel sequencing(MPS)in our casework,we analysed a total of 33 Y-chromosomal short tandem repeats(Y-STRs)with traditional capillary electrophoresis(CE)and 25 Y-STRs using the newer MPS technology.We studied the outcome of both technologies in 64 father-son pairs using stock and custom-designed kits.Current MPS technology confirmed the 13 mutational events observed with CE and improved our understanding of the complex nature of STR mutations.By detecting isometric sequence variants between unrelated males,we show that sequencing Y-STRs using MPS can boost discrimination power.
基金This work was supported by the National Natural Science Foundation of China[grant number 81401557]This study was funded by ICH-GCP Standardization Construction and Innovation of GCP System[grant number 2018ZX09201018-020].
文摘Short tandem repeats on the Y chromosome(Y-STRs),characterized by paternal inheritance,are valuable in forensic practice.Notably,the potential application of Y-STRs in pedigrees should be drawn upon,especially in China’s surname-concentrated natural villages.The study focused on 50 Y-STRs,including 13 rapidly mutating(RM)Y-STRs that largely constitute the current Y-STR commercial kits,and determined the differences in these Y-STRs between branches in a large pedigree and the discriminatory power of these haplotypes in different units for male relatives.As indicated in the results,14 inconsistencies were observed at 9 Y-STRs between 10 father-son pairs.In addition,these 50 Y-STR haplotypes discriminated 10 out of 47 father-son pairs,106 of 148 cousin pairs,70 of 119 uncle-nephew pairs,17 of 39 brother pairs,and 14 out of 33 grandfather-grandson pairs in a large pedigree.The RM Y-STR set is able to differentiate close male relatives in a large pedigree.
文摘目的评估线性探针方法快速检测结核分枝杆菌的耐药性效果。方法选取2013年-2014年江西省耐药监测点分离培养的阳性菌株,收集菌体,提取DNA,采用MTBDRplus试剂盒进行检测,以国际标准的比例法检测结果作为对照,评价其敏感度及特异度,并分析突变分布情况。结果检测利福平耐药的灵敏度为93.33%,特异度为98.35%;检测异烟肼的灵敏度为76.31%,特异度为98.83%。耐利福平基因突变频率最高的位点为rpo B H531L(57.89%),耐异烟肼基因突变频率最高的位点是kat G S315T1(60.61%)。结论可利用MTBDRplus快速检测利福平和异烟肼的耐药性,是快速发现耐药结核分枝杆菌的有效方法。
