Genetic Diversity of Cephalotaxus mannii, a Rare and Endangered Plant

Using RAPD technique, the DNA diversity of Cephalotaxus mannii Hook. f., its genetic diversity pattern, the reasons for its endangered position and conservative approaches were studied. The results show that: 1. The genetic diversity of C. mannii collected from five localities in Hainan is low, and its adaptability to environmental change is weak. 2. The differences of genetic diversity between intra- and inter-populations are great, and the major variation distributes within the population (DNA diversity is 85.1%). 3. The excessive lumbering, man-made destruction, violent typhoon, edible value of the seeds and genetic drift were the main reasons for the low-level genetic diversity of C. mannii and its endangered position. 4. The difference of the micro-environment and other random factors affecting the population should also be taken into full consideration in the study and in protection of such occasionally scattered plants. 5. Enforced measures should be taken to protect the present population, enlarge the population and lower the loss rate of its gene. Mt. Limulin should be chosen as a conservative spot because of its high genetic diversity and less destruction of the forest. Meanwhile, the protection of other populations should be enforced. 6. The differences within and between the populations are great based on different primers used. The change of proportions in polymorphic loci between the populations is more than that between the primers.

Advance of New Researches on Tissue Culture of Rare and Endangered Plant Elaeagnus mollis

This review summarized the recent research achievements on the process of tissue culture of the rare and endangered plant Elaeagnus mollis,including sterilizing protocols of different explants derived from adult plants and seeds for tube germination,two types of plants regeneration（organ type and organ genesis type）,methods of rooting and transplanting,factors affecting culture,as well as browning and vitrification phenomena and avoiding measures.And the further biotechnology research fields of E.mollis were prospected.

Floristic Characteristics of the Rare and Endangered Plants and the Conservation Countermeasures in Tibet, China

The floristic elements and the geographical distribution are analyzed in thispaper based on statistics of elements of rare and endangered plants in Tibet. The results have beengained as following: (1) According to 'the National Important Wild Conservative Plants List (List1)' and 'the National Important Wild Conservative Plants List (List 1)', there are a total of 54plant species (48 genera and 33 families); (2) The geographical elements are very complicated inTibet with 12 of 15 distribution patterns of genera classified by academician Wu; (3) There areobvious temperate genera with 28 genera accounting for 60. 4% of the total genera; (4) There areabundant endemic species accounting for 18. 52% of total species but poor endemic genera; (5) Thegeographical distribution is uneven and a great of species distribute in the areas between 1 000 mand 3 500 m above sea level; (6) To protect the rare and endangered plants efficiently, sixconservation measures are proposed, and 35 species are suggested for the conservative plants of theautonomous conservation level.

Genetic diversity in Isoetes yunguiensis,a rare and endangered endemic fern in China

Isoetes yunguiensis is an endangered and endem-ic fern in China.Field survey indicated that only one popula-tion and no more than 50 individuals occur in the wild.The genetic variation of 46 individuals from the population remaining at Pingba(Guizhou Province,China)was assessed by Random Amplified Polymorphic DNA(RAPD)fingerprinting.Twelve primers were screened from sixty ten-bp arbitrary primers,and a total of 95 DNA fragments were scored.Of these,62.1%were polymorphic loci,which indi-cated that high level genetic variation existed in the natural population.The accumulation of genetic variation in the history of the taxon and the apparent minimal reduction effect on genetic diversity following destruction of habitat might be responsible for the high level genetic diversity presently remaining in the I.yunguiensis population.However,with the continuing decrease of population size,the genetic diversity will gradually be lost.We suggest that the materials from the extant population should be used for re-establishment of the populations.

Novel evidence from Taxus fuana forests for niche-neutral process assembling community

Background:Understanding the mechanisms underlying community assembly is helpful for conservation and restoration of communities, particularly those that contain rare and endangered species like Taxus fuana, which are endemic to the Western Himalayas. The niche (limiting similarity) vs. neutral (randomness) assembly of the T.fuana forest community in Gyirong County, Tibet, China, was investigated. The net relatedness index (NRI) was calculated using a phylogenetic tree. The phylogenetic characteristics of the community and its relationships with environment were analyzed.Results:The value of the mean NRI at the community level was less than-1.96, indicating that the phylogenetic structure was overdispersed;whereas majority of the NRIs at the tree, shrub, and herb layers were within-1.96 to1.96, indicating random dispersion. Environmental factors accounted for 44.38%, 46.52%, 24.04%, and 14.07%of the variation at the community level, tree, shrub, and herb layer, respectively. The phylogenetic structure at the community level and tree layer were significantly influenced by both topographic and soil factors, while shrub and herb layers tended to be affected by a single environmental factor.Conclusions:Community assembly of the T. fuana forest was simultaneously affected by niche and neutral processes, and their variations were closely related to the environment. Neutral process dominated community assembly in the shrub and herb layers. However, the interaction of limiting similarity and randomness played a dominant role at the community level and tree layer;and contributed to maintenance of biodiversity stability. The synergy of multiple environmental factors had a more obvious influence on community assembly than individual environmental factors, especially at the community level. These findings would help to understand the conservation of rare and endangered tree species, such as T. fuana, in the native community;and highlight the importance of random and non-random processes in assembly and biodiversity maintenance of alpine plant communities.

Evaluating soil nutrients of Dacrydium pectinatum in China using machine learning techniques

Background: The accurate estimation of soil nutrient content is particularly important in view of its impact on plant growth and forest regeneration. In order to investigate soil nutrient content and quality for the natural regeneration of Dacrydium pectinatum communities in China, designing advanced and accurate estimation methods is necessary.Methods: This study uses machine learning techniques created a series of comprehensive and novel models from which to evaluate soil nutrient content. Soil nutrient evaluation methods were built by using six support vector machines and four artificial neural networks.Results: The generalized regression neural network model was the best artificial neural network evaluation model with the smallest root mean square error(5.1), mean error(-0.85), and mean square prediction error(29). The accuracy rate of the combined k-nearest neighbors(k-NN) local support vector machines model(i.e. k-nearest neighbors-support vector machine(KNNSVM)) for soil nutrient evaluation was high, comparing to the other five partial support vector machines models investigated. The area under curve value of generalized regression neural network(0.6572) was the highest, and the cross-validation result showed that the generalized regression neural network reached 92.5%.Conclusions: Both the KNNSVM and generalized regression neural network models can be effectively used to evaluate soil nutrient content and quality grades in conjunction with appropriate model variables. Developing a new feasible evaluation method to assess soil nutrient quality for Dacrydium pectinatum, results from this study can be used as a reference for the adaptive management of rare and endangered tree species. This study, however, found some uncertainties in data acquisition and model simulations, which will be investigated in upcoming studies.