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An improved method to distinguish between scanned and rasterized document images
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作者 SUN Juelu 《Baosteel Technical Research》 CAS 2014年第1期58-62,共5页
Office automation (OA) has evolved with the development of computer science,improving staff efficiency.Unstructured information processing is an important aspect of OA; therefore,in this paper,we propose an efficien... Office automation (OA) has evolved with the development of computer science,improving staff efficiency.Unstructured information processing is an important aspect of OA; therefore,in this paper,we propose an efficient method for distinguishing scanned and rasterized document images which can be used in this process.To ensure the efficiency and precision of our method,two steps are included:rapid processing and classification using noise features.In the first step,color,skew,and isolated noise features are used to identify the source of the images.In the second step,noise features are extracted from the input image and a support vector machine (SVM) classifier is used for classification.Our experiments show that our method has high precision and speed for distinguishing scanned and rasterized document images. 展开更多
关键词 office automation unstructured information processing scanned image rasterized image noise extraction
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基于Oracle 10g的栅格数据共享机制研究与实现 被引量:2
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作者 胡刚林 苗放 +3 位作者 王权海 杜根远 谭磊 晏金 《信息技术》 2009年第1期42-46,共5页
空间栅格数据共享机制为遥感数据共享提供信息支持,它的建立和实现可以极大地促进空间数据交换、拓展遥感数据的应用范围。该机制采用了Oracle 10g新增的栅格数据类型GeoRaster提供的功能和方法,并结合Commons-FileUpload处理文件上传,F... 空间栅格数据共享机制为遥感数据共享提供信息支持,它的建立和实现可以极大地促进空间数据交换、拓展遥感数据的应用范围。该机制采用了Oracle 10g新增的栅格数据类型GeoRaster提供的功能和方法,并结合Commons-FileUpload处理文件上传,FileInputStream类和FileOutput-Stream类分别处理文件读写访问,实现了栅格数据以Web方式共享,并可根据客户的需求由栅格金字塔产生不同分辨率的栅格数据。应用结果证明该机制推广方便,解决了大文件栅格数据共享困难的问题。 展开更多
关键词 数据共享机制 SDO—GEORASTER SDO—RASTER 影像金字塔
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Raster flite视景系统数据库建模分析
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作者 陈又军 苏彬 米祖强 《中国民航飞行学院学报》 2009年第6期16-19,共4页
视景系统为飞行员提供虚拟的外部世界,它是模拟飞行仿真的关键技术之一。视景系统是否真实取决于机场视景数据库的建模逼真度。本文分析了Raster flite视景系统数据库的特点、开发过程和在全任务飞行模拟机上的安装、调试方法。其主要... 视景系统为飞行员提供虚拟的外部世界,它是模拟飞行仿真的关键技术之一。视景系统是否真实取决于机场视景数据库的建模逼真度。本文分析了Raster flite视景系统数据库的特点、开发过程和在全任务飞行模拟机上的安装、调试方法。其主要开发工具采用Multigen Creator、Photoshop和Raster flite视景数据库开发软件包。 展开更多
关键词 RASTER flite 视景系统 机场视景数据库 模拟机 飞行仿真
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SUN工作站图像处理的基本问题研究
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作者 范劲松 《佛山科学技术学院学报(社会科学版)》 1996年第4期51-55,共5页
通过对工作站上图像处理问题的研究.给出了SUN工程工作站上raster图像文件的文对头、映象表、图像数据等的结构形式.分析了涉及raster图像文件显示的问题,讨论了pixrects中与raster图像处理有关的过程... 通过对工作站上图像处理问题的研究.给出了SUN工程工作站上raster图像文件的文对头、映象表、图像数据等的结构形式.分析了涉及raster图像文件显示的问题,讨论了pixrects中与raster图像处理有关的过程.以及在工作站上X—Windows系统中显示图像信息的一般流程。 展开更多
关键词 图像处理 raster文件 Pixrect图形库 SUN工作站
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不同激光束直径对LA-ICP-MS检测泥土的准确性影响分析 被引量:1
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作者 史洪飞 郭洪玲 权养科 《中国西部科技》 2011年第28期14-15,共2页
目的:通过试验找出不同模式下,适合用于电感耦合等离子体质谱与激光联用技术检测泥土的激光束直径。方法:在raster取样方法和线取样方法下,分别取激光束直径为25um,50um,100um,150um,200um,对标准土样GSS-1到GSS-8进行检测,并对ICP计数... 目的:通过试验找出不同模式下,适合用于电感耦合等离子体质谱与激光联用技术检测泥土的激光束直径。方法:在raster取样方法和线取样方法下,分别取激光束直径为25um,50um,100um,150um,200um,对标准土样GSS-1到GSS-8进行检测,并对ICP计数、样品相应元素测试准确性和标准曲线的相关系数三个方面进行分析。结果:在raster取样方法和线取样方法下,激光束直径为150um时测量值准确,相关系数均在0.99以上。结论:150um的直径最为合适。 展开更多
关键词 LA—ICP—MS 直径 raster取样方法 线取样方法
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Optimized cellular automaton for stand delineation 被引量:4
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作者 Timo Pukkala 《Journal of Forestry Research》 SCIE CAS CSCD 2019年第1期107-119,共13页
Forest inventories based on remote sensing often interpret stand characteristics for small raster cells instead of traditional stand compartments. This is the case for instance in the Lidar-based and multi-source fore... Forest inventories based on remote sensing often interpret stand characteristics for small raster cells instead of traditional stand compartments. This is the case for instance in the Lidar-based and multi-source forest inventories of Finland where the interpretation units are 16 m 9 16 m grid cells. Using these cells as simulation units in forest planning would lead to very large planning problems. This difficulty could be alleviated by aggregating the grid cells into larger homogeneous segments before planning calculations. This study developed a cellular automaton(CA) for aggregating grid cells into larger calculation units, which in this study were called stands. The criteria used in stand delineation were the shape and size of the stands, and homogeneity of stand attributes within the stand. The stand attributes were: main site type(upland or peatland forest), site fertility, mean tree diameter, mean tree height and stand basal area. In the CA, each cell was joined to one of its adjacent stands for several iterations,until the cells formed a compact layout of homogeneous stands. The CA had several parameters. Due to high number possible parameter combinations, particle swarm optimization was used to find the optimal set of parameter values. Parameter optimization aimed at minimizing within-stand variation and maximizing between-stand variation in stand attributes. When the CA was optimized without any restrictions for its parameters, the resulting stand delineation consisted of small and irregular stands. A clean layout of larger and compact stands was obtained when the CA parameters were optimized with constrained parameter values and so that the layout was penalized as a function of the number of small stands(<0.1 ha). However, there was within-stand variation in fertility class due to small-scale variation in the data. The stands delineated by the CA explained 66–87% of variation in stand basal area, mean tree height and mean diameter, and 41–92% of variation in the fertility class of the site. It was concluded that the CA developed in this study is a flexible new tool,which could be immediately used in forest planning. 展开更多
关键词 FOREST planning Particle SWARM OPTIMIZATION RASTER data Segmentation Spatial OPTIMIZATION
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HUBEX强化观测期雷达测雨在水文过程模拟中的应用(英) 被引量:4
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作者 任立良 李春红 王美荣 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2003年第2期205-212,共8页
On the basis of Digital Elevation Model data, the raster flow vectors, watershed delineation, and spatial topological relationship are generated by the Martz and Garbrecht method for the upper area of Huangnizhuang st... On the basis of Digital Elevation Model data, the raster flow vectors, watershed delineation, and spatial topological relationship are generated by the Martz and Garbrecht method for the upper area of Huangnizhuang station in the Shihe Catchment with 805 km<SUP>2</SUP> of area, an intensified observation field for the HUBEX/GAME Project. Then, the Xin’anjiang Model is applied for runoff production in each grid element where rain data measured by radar at Fuyang station is utilized as the input of the hydrological model. The elements are connected by flow vectors to the outlet of the drainage catchment where runoff is routed by the Muskingum method from each grid element to the outlet according to the length between each grid and the outlet. The Nash-Sutcliffe model efficiency coefficient is 92.41% from 31 May to 3 August 1998, and 85.64%, 86.62%, 92.57%, and 83.91%, respectively for the 1st, 2nd, 3rd, and 4th flood events during the whole computational period. As compared with the case where rain-gauge data are used in simulating the hourly hydrograph at Huangnizhuang station in the Shihe Catchment, the index of model efficiency improvement is positive, ranging from 27.56% to 69.39%. This justifies the claim that radar-measured data are superior to rain-gauge data as inputs to hydrological modeling. As a result, the grid-based hydrological model provides a good platform for runoff computation when radar-measured rain data with highly spatiotemporal resolution are taken as the input of the hydrological model. 展开更多
关键词 digital elevation model RASTER CATCHMENT RADAR hydrological processes modeling
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Spatial variation of the relationship between transport accessibility and the level of economic development in Qinghai-Tibet Plateau, China 被引量:4
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作者 YANG Xue-ting QIU Xiao-ping +2 位作者 FANG Yi-ping XU Yun ZHU Fu-biao 《Journal of Mountain Science》 SCIE CSCD 2019年第8期1883-1900,共18页
As an important component of China’ transportation systems, for a long time, the insufficient performance of transport in QinghaiTibet Plateau(QTP) was a bottleneck restricting the economic growth and social developm... As an important component of China’ transportation systems, for a long time, the insufficient performance of transport in QinghaiTibet Plateau(QTP) was a bottleneck restricting the economic growth and social development in this area. Nevertheless, the implementation of the western development strategy has accelerated the preliminary construction of comprehensive transport network since 2000. Due to the large area and significant geographical heterogeneity, there is a growing need to understand the relationship between transportation and economic development based on the perspective of spatial difference. By using GIS-based raster analysis and Geographically Weighted Regression(GWR) model, we investigated the spatial-temporal distribution of highway, railway and airport accessibility, respectively, and estimated the correlation and heterogeneity between transport accessibility and the level of economic development. Results revealed that:(1) Transport accessibility in the QTP improved by 53.38% in the past 15 years, which is specifically embodied in the improvement of both highway and railway.(2) Accessibility presented prominent differentiation in the space, increasing from west to east and reducing with the rise of elevation, specifically, the best accessibility area of the highway is below 4000 m above sea level, while the area with an altitude of over 4000 m has the lowest aviation time cost.(3) In general, the long weighted average time cost to critical transport facilities posed significantly negative effect on county economic growth in QTP, more positively, the adverse effect gradually weakened over time.(4) Obvious heterogeneity exists at the influence of different transport accessibility factors on the level of economic development, reflecting both in the horizontal space and altitudinal belt. Therefore, region-specific policies should be addressed for the sustainable development of transport facilities as well as economy in the west mountain areas. 展开更多
关键词 ACCESSIBILITY RASTER cost WEIGHTED Geographically WEIGHTED regression Economic effect Spatial variations
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4D DATA FUSION TECHNIQUE IN URBAN WATERLOG-DRAINING DECISION SUPPORT SYSTEM 被引量:3
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作者 Li Jun Bian Fuling 《Geo-Spatial Information Science》 2000年第3期42-46,共5页
This paper studies urban waterlog_draining decision support system based on the 4D data fusion technique.4D data includes DEM,DOQ,DLG and DRG.It supplies entire databases for waterlog forecast and analysis together wi... This paper studies urban waterlog_draining decision support system based on the 4D data fusion technique.4D data includes DEM,DOQ,DLG and DRG.It supplies entire databases for waterlog forecast and analysis together with non_spatial fundamental database.Data composition and reasoning are two key steps of 4D data fusion.Finally,this paper gives a real case: Ezhou Waterlog_Draining Decision Support System (EWDSS) with two application models,i.e.,DEM application model,water generating and draining model. 展开更多
关键词 4D DATA FUSION RASTER VECTOR
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Using ALS raster data in forest planning 被引量:2
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作者 Timo Pukkala 《Journal of Forestry Research》 SCIE CAS CSCD 2019年第5期1581-1593,共13页
Raster type of forest inventory data with site and growing stock variables interpreted for small squareshaped grid cells are increasingly available for forest planning.In Finland,there are two sources of this type of ... Raster type of forest inventory data with site and growing stock variables interpreted for small squareshaped grid cells are increasingly available for forest planning.In Finland,there are two sources of this type of lattice data:the multisource national forest inventory and the inventory that is based on airborne laser scanning(ALS).In both cases,stand variables are interpreted for 16 m×16 m cells.Both data sources cover all private forests of Finland and are freely available for forest planning.This study analyzed different ways to use the ALS raster data in forest planning.The analyses were conducted for a grid of 375×375 cells(140,625 cells,of which 97,893 were productive forest).The basic alternatives were to use the cells as calculation units throughout the planning process,or aggregate the cells into segments before planning calculations.The use of cells made it necessary to use spatial optimization to aggregate cuttings and other treatments into blocks that were large enough for the practical implementation of the plan.In addition,allowing premature cuttings in a part of the cells was a prerequisite for compact treatment areas.The use of segments led to 5–9%higher growth predictions than calculations based on cells.In addition,the areas of the most common fertility classes were overestimated and the areas of rare site classes were underestimated when segments were used.The shape of the treatment blocks was more irregular in cell-based planning.Using cells as calculation units instead of segments led to 20 times longer computing time of the whole planning process than the use of segments when the number of grid cells was approximately 100,000. 展开更多
关键词 RASTER data ALS-based INVENTORY Spatial optimization Segmentation SIMULATED ANNEALING Cellular AUTOMATA
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AN INTEGRATED DATA MODEL IN THREE DIMENSIONAL GIS 被引量:1
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作者 Gong Jianya Xia Zongguo 《Geo-Spatial Information Science》 1999年第1期1-8,15,共9页
The current GIS can only deal with 2-D or 2.5-D information on the earth surface. A new 3-D data structure and data model need to be designed for the 3-D GIS. This paper analyzes diverse 3-D spatial phenomena from min... The current GIS can only deal with 2-D or 2.5-D information on the earth surface. A new 3-D data structure and data model need to be designed for the 3-D GIS. This paper analyzes diverse 3-D spatial phenomena from mine to geology and their complicated relations, and proposes several new kinds of spatial objects including cross-section, column body and digital surface model to represent some special spatial phenomena like tunnels and irregular surfaces of an ore body. An integrated data structure including vector, raster and object-oriented data models is used to represent various 3-D spatial objects and their relations. The integrated data structure and object-oriented data model can be used as bases to design and realize a 3-D geographic information system. 展开更多
关键词 3-D GEOGRAPHIC information system OBJECT-ORIENTED data model VECTOR RASTER
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新一代天气雷达产品的读取与研究 被引量:1
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作者 李高 《科技视界》 2016年第3期300-300,304,共2页
本文介绍了多普勒雷达产品的一般数据格式,及常用两种产品的扫描方式、读取方式、坐标转换与插值等算法,最后采用实际产品读取验证,结果表明该读取、插值算法适用于产品的读取开发。
关键词 多普勒雷达 RADIAL RASTER 读取
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A New Method of Embedded Fourth Order with Four Stages to Study Raster CNN Simulation 被引量:2
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作者 R. Ponalagusamy S. Senthilkumar 《International Journal of Automation and computing》 EI 2009年第3期285-294,共10页
A new Runge-Kutta (PK) fourth order with four stages embedded method with error control is presentea m this paper for raster simulation in cellular neural network (CNN) environment. Through versatile algorithm, si... A new Runge-Kutta (PK) fourth order with four stages embedded method with error control is presentea m this paper for raster simulation in cellular neural network (CNN) environment. Through versatile algorithm, single layer/raster CNN array is implemented by incorporating the proposed technique. Simulation results have been obtained, and comparison has also been carried out to show the efficiency of the proposed numerical integration algorithm. The analytic expressions for local truncation error and global truncation error are derived. It is seen that the RK-embedded root mean square outperforms the RK-embedded Heronian mean and RK-embedded harmonic mean. 展开更多
关键词 Raster scheme cellular neural network (CNN) numerical integration techniques edge detection new embedded RungeKutta root mean square (RKARMS (4 4)) method truncation errors.
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PENETRATION RATE MEASUREMENT AND ITS INSTRUMENT DESIGN
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作者 Qiu Zhenyuan 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 1991年第1期14-19,共6页
The practical method for precise penetration rate measurement in a short time and the instrument design have been specially discussed in this paper.Considering the defects of the penetration rate meters available,via ... The practical method for precise penetration rate measurement in a short time and the instrument design have been specially discussed in this paper.Considering the defects of the penetration rate meters available,via the balanced design in consideration of service life,costs,accuracy and reliability,a kind of penetration rate meter has been put forward.The meter consists of a magnetic clutch,a gear box and a raster disk system to pick up the signal.The meter has the following advantages-it might measure a low speed with higher accura-cy,it could disengage automatically without being divorced from the driving device during tripping rods at high speed,it has a circuit of rotary direction discrimination and of repeated counting prevention so as to eU-minate error caused by rod vibration. 展开更多
关键词 penetration rate measurement instrument design magnetic clutch gear box raster disk
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Shortening the delivery time of proton therapy by real-time compensation method with raster scanning
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作者 Xiang-Shang Sun Yong-Jiang Li +3 位作者 Jun-Ya Liu Wen-Tao Liao Chao Wu Yue-Hu Pu 《Nuclear Science and Techniques》 SCIE EI CAS CSCD 2022年第6期62-74,共13页
Among the various scanning techniques, spot and raster scanning are the most frequently adopted. Raster scanning turns off the beam only when each isoenergy slice irradiation is completed. This feature intrinsically s... Among the various scanning techniques, spot and raster scanning are the most frequently adopted. Raster scanning turns off the beam only when each isoenergy slice irradiation is completed. This feature intrinsically solves the leakage dose and frequent beam-switching problems encountered during spot scanning. However, to shorten the delivery time of raster scanning, a sophisticated dose control strategy is required to guarantee dose distribution.In this study, a real-time compensation method with raster scanning for synchrotron systems was designed. It is characterized by a small spot-spacing planning strategy and real-time subtraction of the transient number of particles delivered between two planning-spot positions from the planned number of particles of the subsequent raster point.The efficacy of the compensation method was demonstrated by performing accurate raster scanning simulations with an in-house simulation code and accurate final dose evaluations with a commercial treatment planning system.Given the similar dose evaluation criteria under a practical high scanning speed, compared with the spot scanning method, the total delivery time of the compensated raster scanning method was significantly shortened by 53.3% in the case of irradiating a cubical target and by 28.8% in a pelvic case. Therefore, it can be concluded that real-time compensated raster scanning with a fast scanning configuration can significantly shorten the delivery time compared to that of spot scanning. It is important to reduce the pressure on patients caused by prolonged immobilization and to improve patient throughput capacity at particle therapy centers. 展开更多
关键词 Proton therapy Raster scanning Delivery time Scanning simulation
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An enhanced method for estimating snow water equivalent in the central part of the Tibetan Plateau using raster segmentation and eigenvector spatial filtering regression model
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作者 CHENG Qi-shan CHEN Yu-min +3 位作者 YANG Jia-xin CHEN Yue-jun XIONG Zhe-xin ZHOU An-nan 《Journal of Mountain Science》 SCIE CSCD 2022年第9期2570-2586,共17页
Snow water equivalent(SWE)is an important factor reflecting the variability of snow.It is important to estimate SWE based on remote sensing data while taking spatial autocorrelation into account.Based on the segmentat... Snow water equivalent(SWE)is an important factor reflecting the variability of snow.It is important to estimate SWE based on remote sensing data while taking spatial autocorrelation into account.Based on the segmentation method,the relationship between SWE and environmental factors in the central part of the Tibetan Plateau was explored using the eigenvector spatial filtering(ESF)regression model,and the influence of different factors on the SWE was explored.Three sizes of 16×16,24×24 and 32×32 were selected to segment raster datasets into blocks.The eigenvectors of the spatial adjacency matrix of the segmented size were selected to be added into the model as spatial factors,and the ESF regression model was constructed for each block in parallel.Results show that precipitation has a great influence on SWE,while surface temperature and NDVI have little influence.Air temperature,elevation and surface temperature have completely different effects in different areas.Compared with the ordinary least square(OLS)linear regression model,geographically weighted regression(GWR)model,spatial lag model(SLM)and spatial error model(SEM),ESF model can eliminate spatial autocorrelation with the highest accuracy.As the segmentation size increases,the complexity of ESF model increases,but the accuracy is improved. 展开更多
关键词 Snow water equivalent Tibetan Plateau Raster segmentation Parallel eigenvector spatial filtering
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An easy approach to assess the susceptibility of a landslide by utilizing simple raster overlay methods: A case study on Huangtupo landslide(P.R. China)
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作者 Christian DUMPERTH Joachim ROHN +3 位作者 Alexander FLEER WANG Jin-ge XIANG Wei Karsten ZIMMERMANN 《Journal of Mountain Science》 SCIE CSCD 2016年第10期1701-1710,共10页
Semi qualitative index based methods using rankings and ratings are commonly used in susceptibility estimations over a wide area. However, generalized ranking and ratings are not applicable for one single landslide. T... Semi qualitative index based methods using rankings and ratings are commonly used in susceptibility estimations over a wide area. However, generalized ranking and ratings are not applicable for one single landslide. This paper gives an easy and transferable approach to a susceptibility assessment of Huangtupo landslide(P.R. China), using raster addition without taking account for ranking and ratings. Slope, aspect, curvature, location and drainage buffer distance raster data sets have been obtained out of open source digital elevation models using ESRI's Arc GIS. These conditioning factor raster data sets have been translated into raster data sets including simple yes or no criteria, referring to triggering or not. Subsequently they have been added by raster math to acquire a simple raster overlay map.After that this map is compared to initial displacement measurements, obtained by using a ground based synthetic aperture radar device. Acquired data is recalculated to a raster data set using the same spatial extent, to provide the possibility of comparison of the two raster data sets. The results reveal, that 76.35% of all measured movements occur in areas where raster cells include three or more conditioning factors, indicating that easy raster math operations can lead to satisfying results in local scale. 展开更多
关键词 Landslide susceptibility Geographical Information System(GIS) Raster overlay Huangtupo Radar interferometry
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Quantitative Study on the Relationship between Arable Land and Its Influencing Factors in Southern Loess Plateau
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作者 Bin GUO Lin PEI 《Asian Agricultural Research》 2015年第2期92-96,共5页
With the Southern Loess Plateau as the object of study,we select the nonbiological factors( physical factors),biological factors and human factors that affect the landscape of arable land to build indicator system. Us... With the Southern Loess Plateau as the object of study,we select the nonbiological factors( physical factors),biological factors and human factors that affect the landscape of arable land to build indicator system. Using GIS,we perform the visualization expression and hierarchical storage of influencing factors to build 1 km × 1 km integrated vector and raster database of arable land landscape pattern and its influencing factors. Using spatial regression analysis,we determine the quantitative relationship between arable land landscape and its influencing factors. The results show that the arable land in the Southern Loess Plateau is mainly distributed in the regions with high temperature,great average annual precipitation,high altitude,high soil N content,small slope,GDP per unit area of land,low ≥10℃ accumulated temperature,and short distance away from the rivers and roads. The study provides a scientific basis for clarifying the relationship between arable land landscape and its influencing factors. 展开更多
关键词 ARABLE LAND INTEGRATED VECTOR and RASTER data LAND
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Method for Automated Georeferencing and Integrating Printed Maps in GIS for Collecting Addresses
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作者 Mohamed El Imame Malaainine Hassane Rhinane +1 位作者 Lahcen Baidder Omar Bachir Alami 《Journal of Geographic Information System》 2013年第1期33-39,共7页
Addresses are important data for urban applications. About 80% of the information local authorities use have a geographic component that is generally related to addresses. Addressing systems efficiency depend on the q... Addresses are important data for urban applications. About 80% of the information local authorities use have a geographic component that is generally related to addresses. Addressing systems efficiency depend on the quality of addresses locators. There are several methods to collect data. Surveys from the field are essential: GPS and pre-printed maps can be used to achieve this goal. GPS surveys from the field may be a solution, but it remains practical only for limited areas. To insure an accepted accuracy, GPS methods need special considerations that are time and money consuming. For Casablanca’s addressing locators, an alternative approach was adopted to collect 400 000 points. It took two months, 200 operators and 3500 printed maps to cover a study area of1,226 km2. This paper is to develop an optimized approach based on automated procedure for reintegrating printed maps in a geographic information system (GIS). It saves georeferencing time from 5min to just seconds per document. It insures, more importantly, an accuracy that is between20 cmto1 mfor scales that are between 1/500 and 1/2500. It ensures maps’ integration, independently of base map and coordinates system by introducing the notion of Georeferencing Code (GC). 展开更多
关键词 GIS ADDRESSING Reference GEOREFERENCING GEODATABASE RASTER Data NAC GC
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Measurement of Protein 53 Diffusion Coefficient in Live HeLa Cells Using Raster Image Correlation Spectroscopy (RICS)
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作者 Sungmin Hong Ying-Nai Wang +5 位作者 Hirohito Yamaguchi Harinibytaraya Sreenivasappa Chao-Kai Chou Pei-Hsiang Tsou Mien-Chie Hung Jun Kameoka 《Journal of Biomaterials and Nanobiotechnology》 2010年第1期31-36,共6页
We have applied Raster Image Correlation Spectroscopy (RICS) technique to characterize the dynamics of protein 53 (p53) in living cells before and after the treatment with DNA damaging agents. HeLa cells expressing Gr... We have applied Raster Image Correlation Spectroscopy (RICS) technique to characterize the dynamics of protein 53 (p53) in living cells before and after the treatment with DNA damaging agents. HeLa cells expressing Green Fluores-cent Protein (GFP) tagged p53 were incubated with and without DNA damaging agents, cisplatin or eptoposide, which are widely used as chemotherapeutic drugs. Then, the diffusion coefficient of GFP-p53 was determined by RICS and it was significantly reduced after the drug treatment while that of the one without drug treatment was not. It is suggested that the drugs induced the interaction of p53 with either other proteins or DNA. Together, our results demonstrated that RICS is able to detect the protein dynamics which may be associated with protein-protein or protein-DNA interactions in living cells and it may be useful for the drug screening. 展开更多
关键词 RASTER Image Correlation SPECTROSCOPY DIFFUSION COEFFICIENT p53 DNA DAMAGE
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