AIM：To investigate the intestine and body development of intrauterine growth retardation（IUGR）rats under early different protein diet and to analyze the correlation between leptin and intestine and body development...AIM：To investigate the intestine and body development of intrauterine growth retardation（IUGR）rats under early different protein diet and to analyze the correlation between leptin and intestine and body development．METHODS：An IUGR rat model was established by food restriction of pregnant female rats．Fifty-six neonatal IUGR rats and 24 neonatal normal rats were randomly divided into normal control group（Cgroup），IUGR model group （scgroup），low protein diet IUGR group（SL group），and high protein diet IUGR group（SH group）．Eight rats were killed per group at wk 0，4，and 12．Serum leptin，body weight（BW），body length（BL），intestinal weight（IW），intestinal length（IL），andintestinal disaccharidase（including lactase，maltase，and saccharase） were detected．RESULTS：BW（4．50±0．41g），BL（5．96±0．40cm），IW（0．05±0．01g），and IL（15.9±2．8cm）in neonatal IUGR rats were much lower than those in Cgroup（6．01±0．55g，6．26±0．44cm，0．10±0．02g，21.8±2．7cm，P〈0．05），while intestinal lactase and maltase activities were higher than those in Cgroup．SH group showed the fastest catch up growth and their BW，BL，IW，and IL reached the Cgroup level at wk 4．SC group showed relatively slower catch up growth than SH group，and their BW，BL，IW did not reach the Cgroup level at wk 4．SL group did not show intestine and body catch up growth．Intestinal maltase [344±33μmol／（min·q）]and saccharase activities[138±32μmol／（min·g）]in SL group were both markedly lower than nose in C group [751±102，258±271μmol／（min·g），P〈0．05]．There were no significant difierences in lactase activities at wk 4 and disaccharidase activities at wk 12 among all groups（P〈0．05）．The leptin level in SL group（0．58±0．12ng／mL） was the highest in all groups，and much lower in SH group（0．21±0．03ng／mL） than that in any other IUGR groups at wk 4（P〈0．05）．Leptin was negatively related to BW （r=-0．556，P=0．001），IW（r=-0．692，P=0．001） and IL（r=-0．738，P=0．000）at wk 4，while no correlation was found at wk 12．CONCLUSION：High protein diet is a reasonable early nutritional mode to IUGR rats in promoting intestine and body catch up growth．展开更多
The rats exposed to passive smoking were used as the animal model to further study the pathogenesis of IUGR and the effects of ' Huoxuequyu' decoction. The results showed that the mean birth-weight of fetus an...The rats exposed to passive smoking were used as the animal model to further study the pathogenesis of IUGR and the effects of ' Huoxuequyu' decoction. The results showed that the mean birth-weight of fetus and SOD activity of placenta and plasma in model rats were significantly reduced, and LPO levels of placenta and plasma were significantly increased. We found that the mean birthweight and SOD activity in treated group were higher than that in model group,and LPO levels of placenta and plasma were lower in treated group than that in model group and there was no difference in SOD activity and LPO levels of placenta and plasma as well as the mean birthweight between treated group and control group, indicating that 'Huoxuequyu' decoction can protect placenta and plasma against lipid peroxidation, and improve the blood circulation of uteroplacenta, thus contributing to the increase of the mean birth-weight.展开更多
目的研究胰岛素受体底物(IRS)-1和IRS-2在生长追赶宫内发育迟缓新生大鼠脂肪细胞中的时序性表达及其与胰岛素抵抗的关联。方法通过母鼠孕期全程饲料限制建立宫内发育迟缓模型,相对增加哺乳期子鼠营养摄入实现生长追赶。于4周龄时心脏采...目的研究胰岛素受体底物(IRS)-1和IRS-2在生长追赶宫内发育迟缓新生大鼠脂肪细胞中的时序性表达及其与胰岛素抵抗的关联。方法通过母鼠孕期全程饲料限制建立宫内发育迟缓模型,相对增加哺乳期子鼠营养摄入实现生长追赶。于4周龄时心脏采血测定空腹血糖、胰岛素和三酰甘油,计算胰岛素抵抗指数。测定1、3、5、7周龄SD大鼠的成熟脂肪细胞及前脂肪细胞分化的成熟脂肪细胞的IRS-1和IRS-2 m RNA以及蛋白水平的变化。结果生长追赶的宫内发育迟缓新生大鼠原代培养的成熟脂肪细胞IRS-1和IRS-2 m RNA表达分别于生后第5周和第7周显著下调,差异有统计学意义(P<0.05);前脂肪细胞分化的成熟脂肪细胞IRS-1和IRS-2 m RNA表达均于生后第5周显著下调,差异有统计学意义(P<0.05);两种成熟脂肪细胞IRS-1和IRS-2蛋白水平均于生后第1周开始显著下调,差异有统计学意义(P<0.05)。结论生长追赶的宫内发育迟缓新生大鼠脂肪细胞IRS-1和IRS-2的基因表达发生相对下调,可能与其生后远期的胰岛素抵抗密切相关。展开更多
基金Supported Dy the Science and Technology Bureau Foundation of Guangdong Province, No. 99M04815G
文摘AIM：To investigate the intestine and body development of intrauterine growth retardation（IUGR）rats under early different protein diet and to analyze the correlation between leptin and intestine and body development．METHODS：An IUGR rat model was established by food restriction of pregnant female rats．Fifty-six neonatal IUGR rats and 24 neonatal normal rats were randomly divided into normal control group（Cgroup），IUGR model group （scgroup），low protein diet IUGR group（SL group），and high protein diet IUGR group（SH group）．Eight rats were killed per group at wk 0，4，and 12．Serum leptin，body weight（BW），body length（BL），intestinal weight（IW），intestinal length（IL），andintestinal disaccharidase（including lactase，maltase，and saccharase） were detected．RESULTS：BW（4．50±0．41g），BL（5．96±0．40cm），IW（0．05±0．01g），and IL（15.9±2．8cm）in neonatal IUGR rats were much lower than those in Cgroup（6．01±0．55g，6．26±0．44cm，0．10±0．02g，21.8±2．7cm，P〈0．05），while intestinal lactase and maltase activities were higher than those in Cgroup．SH group showed the fastest catch up growth and their BW，BL，IW，and IL reached the Cgroup level at wk 4．SC group showed relatively slower catch up growth than SH group，and their BW，BL，IW did not reach the Cgroup level at wk 4．SL group did not show intestine and body catch up growth．Intestinal maltase [344±33μmol／（min·q）]and saccharase activities[138±32μmol／（min·g）]in SL group were both markedly lower than nose in C group [751±102，258±271μmol／（min·g），P〈0．05]．There were no significant difierences in lactase activities at wk 4 and disaccharidase activities at wk 12 among all groups（P〈0．05）．The leptin level in SL group（0．58±0．12ng／mL） was the highest in all groups，and much lower in SH group（0．21±0．03ng／mL） than that in any other IUGR groups at wk 4（P〈0．05）．Leptin was negatively related to BW （r=-0．556，P=0．001），IW（r=-0．692，P=0．001） and IL（r=-0．738，P=0．000）at wk 4，while no correlation was found at wk 12．CONCLUSION：High protein diet is a reasonable early nutritional mode to IUGR rats in promoting intestine and body catch up growth．
文摘The rats exposed to passive smoking were used as the animal model to further study the pathogenesis of IUGR and the effects of ' Huoxuequyu' decoction. The results showed that the mean birth-weight of fetus and SOD activity of placenta and plasma in model rats were significantly reduced, and LPO levels of placenta and plasma were significantly increased. We found that the mean birthweight and SOD activity in treated group were higher than that in model group,and LPO levels of placenta and plasma were lower in treated group than that in model group and there was no difference in SOD activity and LPO levels of placenta and plasma as well as the mean birthweight between treated group and control group, indicating that 'Huoxuequyu' decoction can protect placenta and plasma against lipid peroxidation, and improve the blood circulation of uteroplacenta, thus contributing to the increase of the mean birth-weight.
文摘目的研究胰岛素受体底物(IRS)-1和IRS-2在生长追赶宫内发育迟缓新生大鼠脂肪细胞中的时序性表达及其与胰岛素抵抗的关联。方法通过母鼠孕期全程饲料限制建立宫内发育迟缓模型,相对增加哺乳期子鼠营养摄入实现生长追赶。于4周龄时心脏采血测定空腹血糖、胰岛素和三酰甘油,计算胰岛素抵抗指数。测定1、3、5、7周龄SD大鼠的成熟脂肪细胞及前脂肪细胞分化的成熟脂肪细胞的IRS-1和IRS-2 m RNA以及蛋白水平的变化。结果生长追赶的宫内发育迟缓新生大鼠原代培养的成熟脂肪细胞IRS-1和IRS-2 m RNA表达分别于生后第5周和第7周显著下调,差异有统计学意义(P<0.05);前脂肪细胞分化的成熟脂肪细胞IRS-1和IRS-2 m RNA表达均于生后第5周显著下调,差异有统计学意义(P<0.05);两种成熟脂肪细胞IRS-1和IRS-2蛋白水平均于生后第1周开始显著下调,差异有统计学意义(P<0.05)。结论生长追赶的宫内发育迟缓新生大鼠脂肪细胞IRS-1和IRS-2的基因表达发生相对下调,可能与其生后远期的胰岛素抵抗密切相关。
