AIM To study the short term effect of Danshen ( Salvia miltiorrhiza ) on acetic acid induced chronic gastric ulcer in rats and its long term effect in preventing recurrence. METHODS Rats with acetic acid indu...AIM To study the short term effect of Danshen ( Salvia miltiorrhiza ) on acetic acid induced chronic gastric ulcer in rats and its long term effect in preventing recurrence. METHODS Rats with acetic acid induced gastric ulcer were treated with Danshen and cimetidine for 30 days. Traditional gastric mucosal auto radiography and 3H TdR incorporation into gastric mucosa in vitro were employed to study the effects of Danshen in rat acetic acid induced chronic gastric ulcer, including ulcer index (UI), ulcer inhibitory rate (IR) and label rate (LR). RESULTS On the day 5, 30 and 126 of ulcer making, the UI in the Danshen group was obviously lower than that in the cimetidine group and the control group (42 3±3 9, 3 6±1 2, 4 4±2 3; 49 1±3 6, 5 9±1 4, 9 2±1 3; 61 0±3 8, 8 9±2 5, 12 4±2 4, respectively, P <0 01), the IR (%) in the Danshen group was obviously higher than that in the cimetidine group (31, 59, 64 8; 19, 33, 26, respectively), and the LR in the Danshen group was obviously higher than that in the cimetidine group and the control group (10 0±0 5, 16 2±0 8, 15 0±0 6; 9 0±0 5, 13 9±0 6, 10 8±0 7; 6 5±0 7, 10 1±0 5, 8 0±0 7, respectively, P <0 01). There was no obvious difference in UI in the Danshen group on day 30 as compared with that on day 126. CONCLUSION Danshen is effective in promoting ulcer healing and preventing recurrence. The mechanism of action is to strengthen the gastric mucosal barrier and to promote the gastric mucosal cell proliferation along the edge of the ulcer.展开更多
AIM: To investigate the relationship among gastrin, somatostatin, G and D cells in gastric ulcer and in its healing process in rats. METHODS: Fourty-nine Wistar rats were divided into 7 groups. The gastric ulcer model...AIM: To investigate the relationship among gastrin, somatostatin, G and D cells in gastric ulcer and in its healing process in rats. METHODS: Fourty-nine Wistar rats were divided into 7 groups. The gastric ulcer model was induced by acetic acid successfully. The gastrin and the somatostatin in rat plasma, gastric fluid and antral tissue were measured by radioimmunoassay(RIA). G and D cells in antral mucosa were analyzed with polyclonal antibody of gastrin and somatostatin by immunohistochemical method and Quantimet 500 image analysis system. RESULTS: In gastric ulcer, the level of gastrin in plasma, gastric fluid, and antral tissue increased, that of somatostatin declined, and the disorder gradually recovered to the normal level in the healing process. Immunohistochemical technique of G and D cells in antral mucosa demonstrated that the number of G cells increased and that of D cells decreased, both areas of G and D cells declined, the ratio of number and area of G/D increased in gastric ulcer, and the disorder gradually recovered in the healing process. CONCLUSION: In gastric ulcer, the increased gastrin secreted by G cells, the declined somatostatin secreted by D cells, and the disordered G/D cell ratio can lead to gastrointestinal dysfunction.展开更多
目的探讨甘松不同提取成分对大鼠急性胃炎的预防作用,为临床用药提供依据。方法将W istar大鼠完全随机分成生理盐水组、甘松水提浸膏高剂量组、甘松水提浸膏高剂量加挥发油高剂量组、甘松水提浸膏高剂量与挥发油低剂量组、甘松水提浸膏...目的探讨甘松不同提取成分对大鼠急性胃炎的预防作用,为临床用药提供依据。方法将W istar大鼠完全随机分成生理盐水组、甘松水提浸膏高剂量组、甘松水提浸膏高剂量加挥发油高剂量组、甘松水提浸膏高剂量与挥发油低剂量组、甘松水提浸膏低剂量与挥发油低剂量组及西咪替丁对照组,连续5天,1次/天灌胃给药,末次给药后90分钟,所有动物均灌胃予95%乙醇1 m l只/,观察各药物组预防乙醇所致大鼠急性胃炎及胃溃疡的作用。结果甘松水提浸膏高剂量组、甘松水提浸膏高剂量加挥发油高剂量组、甘松水提浸膏高剂量与挥发油低剂量组、甘松水提浸膏低剂量与挥发油低剂量组在减少胃炎发生方面优于生理盐水组及西咪替丁对照组,其构成比之间差异具有显著的统计学意义(P<0.01);甘松水提浸膏高剂量组、甘松水提浸膏高剂量加挥发油高剂量组、甘松水提浸膏高剂量与挥发油低剂量组的溃疡抑制率分别为37.11%、95.06%、78.38%,高于西咪替丁对照组(35.99%),差异有统计学意义(P<0.05)。结论甘松不同提取成份组合给药对预防急性胃炎及抑制胃溃疡有明显的作用。展开更多
文摘AIM To study the short term effect of Danshen ( Salvia miltiorrhiza ) on acetic acid induced chronic gastric ulcer in rats and its long term effect in preventing recurrence. METHODS Rats with acetic acid induced gastric ulcer were treated with Danshen and cimetidine for 30 days. Traditional gastric mucosal auto radiography and 3H TdR incorporation into gastric mucosa in vitro were employed to study the effects of Danshen in rat acetic acid induced chronic gastric ulcer, including ulcer index (UI), ulcer inhibitory rate (IR) and label rate (LR). RESULTS On the day 5, 30 and 126 of ulcer making, the UI in the Danshen group was obviously lower than that in the cimetidine group and the control group (42 3±3 9, 3 6±1 2, 4 4±2 3; 49 1±3 6, 5 9±1 4, 9 2±1 3; 61 0±3 8, 8 9±2 5, 12 4±2 4, respectively, P <0 01), the IR (%) in the Danshen group was obviously higher than that in the cimetidine group (31, 59, 64 8; 19, 33, 26, respectively), and the LR in the Danshen group was obviously higher than that in the cimetidine group and the control group (10 0±0 5, 16 2±0 8, 15 0±0 6; 9 0±0 5, 13 9±0 6, 10 8±0 7; 6 5±0 7, 10 1±0 5, 8 0±0 7, respectively, P <0 01). There was no obvious difference in UI in the Danshen group on day 30 as compared with that on day 126. CONCLUSION Danshen is effective in promoting ulcer healing and preventing recurrence. The mechanism of action is to strengthen the gastric mucosal barrier and to promote the gastric mucosal cell proliferation along the edge of the ulcer.
基金Natural Science Foundation of Guangdong Province,China,No.010578Important Technological Issue of Guangdong Province,No.99-13
文摘AIM: To investigate the relationship among gastrin, somatostatin, G and D cells in gastric ulcer and in its healing process in rats. METHODS: Fourty-nine Wistar rats were divided into 7 groups. The gastric ulcer model was induced by acetic acid successfully. The gastrin and the somatostatin in rat plasma, gastric fluid and antral tissue were measured by radioimmunoassay(RIA). G and D cells in antral mucosa were analyzed with polyclonal antibody of gastrin and somatostatin by immunohistochemical method and Quantimet 500 image analysis system. RESULTS: In gastric ulcer, the level of gastrin in plasma, gastric fluid, and antral tissue increased, that of somatostatin declined, and the disorder gradually recovered to the normal level in the healing process. Immunohistochemical technique of G and D cells in antral mucosa demonstrated that the number of G cells increased and that of D cells decreased, both areas of G and D cells declined, the ratio of number and area of G/D increased in gastric ulcer, and the disorder gradually recovered in the healing process. CONCLUSION: In gastric ulcer, the increased gastrin secreted by G cells, the declined somatostatin secreted by D cells, and the disordered G/D cell ratio can lead to gastrointestinal dysfunction.
文摘目的探讨甘松不同提取成分对大鼠急性胃炎的预防作用,为临床用药提供依据。方法将W istar大鼠完全随机分成生理盐水组、甘松水提浸膏高剂量组、甘松水提浸膏高剂量加挥发油高剂量组、甘松水提浸膏高剂量与挥发油低剂量组、甘松水提浸膏低剂量与挥发油低剂量组及西咪替丁对照组,连续5天,1次/天灌胃给药,末次给药后90分钟,所有动物均灌胃予95%乙醇1 m l只/,观察各药物组预防乙醇所致大鼠急性胃炎及胃溃疡的作用。结果甘松水提浸膏高剂量组、甘松水提浸膏高剂量加挥发油高剂量组、甘松水提浸膏高剂量与挥发油低剂量组、甘松水提浸膏低剂量与挥发油低剂量组在减少胃炎发生方面优于生理盐水组及西咪替丁对照组,其构成比之间差异具有显著的统计学意义(P<0.01);甘松水提浸膏高剂量组、甘松水提浸膏高剂量加挥发油高剂量组、甘松水提浸膏高剂量与挥发油低剂量组的溃疡抑制率分别为37.11%、95.06%、78.38%,高于西咪替丁对照组(35.99%),差异有统计学意义(P<0.05)。结论甘松不同提取成份组合给药对预防急性胃炎及抑制胃溃疡有明显的作用。
