Long-Term Impact of Acute Retinoic Acid Supplementation at the Young Age on Testicular Architecture of Wistar Albino Rats

Introduction: Inappropriate and excess vitamin supplementation, particularly for vitamin A, is increasingly recognized as a public health problem in developed countries. On the other hand, blind supplementation of vitamin A, for children in developing countries is a subject of controversy in the literature. The crucial role of vitamin A in the process of spermatogenesis in adult rodents is well established, but only a few publications are consecrated to the long-term effect of vitamin A intake at a young age on testicular development and differentiation. Objectives: Our study aimed to evaluate the long-term effects of acute supplementation at an early age, in the post-natal period, on spermatogenesis and testicular trophicity at adult age. Material and Methods: Young Wistar Albinos rats of 22 days received an acute high dose of supplementation of vitamin A (retinyl palmitate). The control group, group 1, received only extra virgin olive oil, Group 2 a dose of 7000 IU/kg of retinyl palmitate, group 3, 14,000 IU/kg, and Group 4 a dose of 28,000 IU/kg. At 10 weeks of age, the testes’ testosterone levels were measured by ELISA. For histological assessment, sections were stained with Hematoxylin eosin, and the Johnsen score was used to evaluate spermatogenesis in the seminiferous tubules. Results: The average testicular weights of rats were significantly lower in group 4 (p < 0.05), and so was the testosterone level in the testis compared to the control group (p .01). Most of the seminiferous tubules were concerned by an arrest of spermatogenesis and the Johnsen score was decreased with a mean score of 5.96 ± 1.60 (p .001) in that Group. In Group 3, Johnsen’s score was significantly better than the one obtained with the control. Conclusion: We observed a negative effect in the long term with a high acute dose of supplementation of retinyl palmitate at a young age, on testicular development and differentiation. Despite a return to normal diet after that supplementation, during childhood, impaired spermatogenesis was identified at the adult age with an arrest of spermatogenesis. The reversibility of that lack of differentiation by a return to a normal diet is questionable and would need more investigation.

Aqueous Leaf Extract of Moringa oleifera (Moringaceae) Effectively Treats Induced Hemolytic Anemia in Wistar Rats

Introduction: Moringa oleifera was a medicinal plant generally used by populations in the food and therapeutic fields. It’s used to treat anemia has been observed in the Djougou Zone in northern Benin. To our knowledge, there were no scientific data available that have evaluated its efficacy in the treatment of haemolytic anemia. This was what justifies this research work in which the phytochemical analysis, extraction and evaluation of the anti-anemic effect were carried out. Methods: Five groups of five Wistar rats each were formed. All the rats were rendered anemic by injection of phenylhydrazine hydrochloride on the first two days D0 and D1 except those in the negative control group. From the second day, the anemic groups were force-fed either with the aqueous extract of Moringa oleifera leaves at 200 or 300 mg/kg body weight/day, or with vitafer, the reference drug against anemia. The positive control group (anemia) was not treated. Blood samples were taken from all the rats on different days: D0, D2, D7, D10 and D15 to evaluate the data of the hemogram and the osmotic resistance of the red blood cells. Results: Phytochemical analysis revealed the presence of tannins, flavonoids, leucoanthocyanins, saponosides, triterpenes and mucilages. A good yield was obtained at the extraction. Both the extract and the reference drug vitafer completely corrected anemia within two weeks after stimulating hemoglobin synthesis and early release of immature red blood cells into the bloodstream. Its effect seemed dose-dependent and specific. Conclusion: Moringa oleifera leaves showed good therapeutic efficacy and can be considered and exploited for transformation into improved traditional medicines (ITM) in the treatment of anemia.

Impact of Selected Processing Methods of High-Level Cyanide in Cassava on Optic Neuropathy in Wistar Albino Rats—An Experimental Study

Background: Cassava tuber crop is a staple food rich in carbohydrates and utilized in various forms by millions of Nigerians. The storage root of the cassava contains linamarin, a cyanogenic glycoside that is easily hydrolyzed to release cyanide salt compounds which is toxic to the nervous system especially the optic nerve, sometimes leading to optic neuropathy and visual impairment. Aim: The aim of this study is to find out the impact of selected processing methods of high-level cyanide in cassava on optic neuropathy in Wistar albino rats. Methodology: Twenty-four Wistar albino rats were fed with different concentration and duration of predetermined high-cyanide content cassava root cultivar which was processed using different processing methods adopted by various communities in Rivers State, Nigeria (for human consumption). A control group of 3 Wistar albino rats was fed with normal “Growth Mesh” meals. The pre and post weights of the animals and the fundoscopic optic nerve status of the rats were evaluated after 30 and 60 days. SPSS Version 25 was employed for descriptive and inferential statistical analyses. A p-value of ≤0.05 was considered statistically significant. Results: The Cassava species available in Rivers State have high cyanide content (2336.79 mg CN-/kg dry weight of cassava). There was statistically significant reduction in the cyanide content (p = 0.000) depending on the various common processing methods (into garri for human consumption): 24 hours, 48 hours, fermentation;with and without red palm oil additive. The individual weights as well as the mean weight of the 24 rats in the experimental group increased gradually from the first week to the 9th week with a slight weight reduction on the third and fourth weeks which was not statistically significant (p = 0.092). However, there was a steady increase in the weights of the animals in the control group throughout the 9 weeks. Varying degrees of optic neuropathy occurred, worse with the rats that had 24-hour fermented cassava twice daily for 60 days. The intra and inter group differences in the optic disc changes was statistically significant (p = 0.000). Conclusion: Longer duration of processing cassava roots into garri for human consumption reduces its cyanide content and minimizes the adverse impact on the optic nerve.

Effects of Sarcocephalus latifolius Fruits Extract on Paracetamol-Induced Liver Damage in Wistar Rats

Background and Aim: Sarcocephalus latifolius is a medicinal plant commonly used in traditional medicine to treat various diseases. The aim of the present study is to evaluate the hepatoprotective activity of Sarcocephalus latifolius fruits aqueous extract against paracetamol-induced liver damage in rats. Material and Methods: Aqueous extract of Sarcocephalus latifolius fruits at doses of 100, 250 and 500 mg/kg were administered orally to rats with paracetamol-induced hepatotoxicity (1 g/kg). The treatment with the extract and paracetamol lasted 7 days. Silymarin (50 mg/kg) was given as reference control. All tested drugs were administered orally. Results: Our results show that the Sarcocephalus latifolius fruits extract induced a significant reduction (p < 0.05) of serum enzymes alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), alkaline phosphatase (PAL) and total bilirubin (TB). Then, the extract at the dose of 500 mg/kg showed a better protection (p < 0.001) of hepatocytes with a percentage of protection of 43.59% ± 2.03%;59.43% ± 4.12%;73.29% ± 5.72% and 62.55% ± 7.48% for ALAT, ASAT, PAL and TB, respectively. The histology of livers exposed to paracetamol shows an inflammation of the hepatocytes. In addition, there was a significant alteration of the liver parenchyma. The 500 mg/kg extract showed a resorption of the inflammation. Histopathological examination showed that the extract regenerated paracetamol-induced liver damage. Conclusion: Aqueous extract of Sarcocephalus latifolius fruits has hepatoprotective activity against paracetamol-induced hepatotoxicity in rats. But it would be important to evaluate the activity of aqueous extract of Sarcocephalus latifolius fruits on oxidative stress parameters in vivo in rats. .

Effects of Total Flavonoids ofHippophae RhamnoidesL.on Intracellular Free Calciumin Cultured Vascular Smooth Muscle Cells of Spontaneously Hypertensive Rats and Wistar-Kyoto Rats

To explore the effects of total flavonoids of Hippophae rhamnoides L. （TFH） quercetin （Que） and isorhamnetin （Isor） on the intracellular free calcium （[Ca^2＋]） in vascular smooth muscle cells （VSMC） of spontaneously hypertensive rats （SHR） and Wistar-Kyoto rats （WKY）. Metheds： Fluo 3-acetoxymethylester（Fluo-3/AM） was used to observe the effects of TFH （100mg/L） and its essential monomers, namely Que （10^-4mol/L） and Isor （10^-4mol/L） on changes of [Ca^2＋]1 in cultured SHR and WKY VSMC （abbr. to Ca-SHR ＆ Ca-WKY） following exposure to high K^＋, norepinephrine （NE） and angiotensin Ⅱ （AngⅡ）, and to compare with the effects of verapamil （Ver）. Results： （1） TFH, Que and Isor had inhibitory effects on resting Ca-SHR （P〈0.05）, but had no significant effects on Ca-WKY （P〉0.05）. （2） High K^＋ could increase Ca-SHR more significantly than Ca-WKY （P〈0.05）; TFH, Que and Isor could inhibit the elevation of [Ca^2＋]1 induced by high K^＋ -depolarization, with the effects similar to that of Ver, and the effect on Ca-SHR was more significant than that on Ca-WKY （P〈0.05）. （3） NE and Ang Ⅱ could increase Ca-SHR more significantly than Ca-WKY （P〈0.05）, TFH, Que and Isor had remarkably inhibitory effect on the elevation of Ca-SHR and Ca-WKY induced by NE or Ang Ⅱ. （4） In the absence of extracellular Ca^2＋ , TFH, Que and Isor also had certain inhibitory effect on Ca-SHR and Ca-WKY induced by NE, and the effect on the former was more significant than that on the latter（P〈0.05）. Ceaclusiea： TFH, Que and Isor might decrease the levels of [Ca^2＋], in VSMCs by blocking both voltage-dependent calcium channels （VDC） and receptoroperated calcium channels （ROC） in physiological or pathological state, which may be one of the important mechanisms of their hypotensive and protective effects on target organs in patients with hypertension.

Radioprotective Potencies of Allium Cepa Extract (ACE) against Radiation-Induced Hepatoxicity in Wistar Rats

Background and Purpose: All types of ionizing radiations generate ions which can lead to the formation of free radicals and reactive oxygen species (ROS). Excess production of free radicals or decrease in antioxidants level leads to oxidative stress. It is a harmful process that induces damage to cell structures, lipids, proteins, RNA and DNA which leads to a number of diseases. The aim of this study is to examine the effect of plant extract (Allium Cepa Extract (ACE)) on the kidney of wistar rats exposed to radiation using and assaying some biochemical enzymes. Material and Method: 60 wistar rats weighing 170 ± 20 g were equally divided into six groups for the study. Group 1 (control): neither received ACE nor irradiation. Group 2 (ACE): received 1000 mg/Kg b.wt of ACE. Group 3 (4 Gy-Irradiated): were exposed to 4 Gy TBI on day 14. Group 4 (6 Gy-Irradiated): were exposed to 6 Gy TBI on day 14. Group 5 (ACE + 4 Gy): were treated with 1000 mg/Kg b.wt of ACE once daily for twenty-eight days but exposed to 4 Gy TBI on day 14. Group 6 (ACE + 6 Gy): were treated with 1000 mg/Kg b.wt of ACE once daily for twenty-eight days but exposed to 6 Gy TBI on day 14. All the groups received distilled water and feed ad libitum during the acclimatization and experimental periods. Four animals in each group were sacrificed 24 h after irradiation and the 4 remaining animals were sacrificed on day 29 for biochemical assay and histopathological evaluation, the statistical analysis was done using one-way analysis of variance (ANOVA) on the data editor SPSS version 28. Results: From the biochemical enzymes, the level of Malondialdehyde (MDA) in group 2 when compared to group 1 was almost the same, which was not statically significant with (p > 0.05), but groups 3 and 4 show a significant increase in the level of MDA with (p < 0.05) while group 5 and 6 showed no significant increase in MAD with (p > 0.05). The other enzymes like SOD, CAT, GST, and GSH followed suit. Conclusion: From the results it is a clear indication that Allium Cepa Extract can ameliorate the effect of radiation induced disease.

Chronic morphine drinking establishes morphine tolerance, but not addiction in Wistar rats

Objective： Some animal models apply morphine in the drinking water to generate addiction, but related reports are not free of conflicting results. Accordingly, this study aimed to figure out if chronic consumption of morphine in the drinking water can induce morphine addiction in Wistar rats. Methods： For 3 weeks, the animals received a daily morphine dose of 35 mg/kg by offering a calculated volume of sugar water （5% sucrose） with morphine （0. l mg/ml） to each rat; animals receiving just sugar water served as controls. Immediately after the treatment phase, the tail immersion test was used to check for morphine tolerance, and all animals were then kept on tap water for one week （withdrawal phase）. Afterwards, all rats were allowed to choose their drinking source by offering two bottles, containing sugar water without and with morphine, simultaneously for two days （preference phase）. Results： While the chronic consumption of morphine led to a reduction in body weight and to morphine tolerance, the morphine-treated Wistar rats did not show any preference for the opiate-containing sugar water. Conclusion： Body weight loss and tolerance do not reveal a condition of drug craving, and current animal models should be re-evaluated regarding their potential to establish morphine addicted animals.

Molsidomine ameliorates diabetic peripheral neuropathy complications in Wistar rats

Diabetic neuropathy is a disorder which affects various regions of the nervous sys-tem and there is no specific treatment available for it.So,the present study eval-uated protective effect of molsidomine in diabetic neuropathy in rats.Diabetes was induced in male Wistar rats by administrating streptozotocin(52 mg/kg i.p).Diabetic rats were administered with molsidomine 5 mg/kg p.o,and 10mg/kg p.o.as treatment.After 8 weeks of treatment,Motor coordination,Mechanical allodynia,Mechanical hyperalgesia,Nerve conduction velocity,Glycosylated hemoglobin were assessed.Thereafter animals were sacrificed and sciatic nerve was isolated.It was used for reduced glutathione,lipid peroxidation and for histopathology.Treatment with molsidomine significantly improved motor coordination,paw withdrawal threshold,mechanical threshold and nerve conduction velocity.Furthermore,mol-sidomine treatment also reduced malondialdehyde levels and prevented depletion of reduced glutathione in sciatic nerve homogenate.Histopathology shows molsid-omine treatment maintained normal architecture of sciatic nerve.The results of our study strengthens the alterative use of molsidomine in diabetic neuropathy.

Acute and subchronic toxicity of hydroxylammonium nitrate in Wistar rats

Hydroxylammonium nitrate(HAN) is a major constituent in a class of liquid monopropellants and is extensively used in nuclear industry and space propulsion.Previous toxicological studies have focused on oral,inhalation and dermal routes of exposure to HAN-based propellant blends.In this study,acute and subchronic toxicity of HAN in Wistar rats by intraperitoneal injections were evaluated.In this acute study,doses of HAN at 115,125,135,147,160 or 174 mg/kg were administered.No adverse effects were observed during a 14-day period and at gross histopathological examination.In the subchronic study,HAN at 7,14 or 28 mg/kg were administered for 13 weeks.The treatment with HAN caused significant changes in the weight of spleen,in the level of hematological parameters,total bilirubin,direct bilirubin,uric acid and carbondioxidecombining power and histopathological damages of the lung,liver,spleen and kidney.Overall,the study suggests that 13-week HAN treatment caused abnormal hematological changes and tissue lesions,and the risk of toxicity to mammals is not negligible.

Curcumin alters motor coordination but not total number of Purkinje cells in the cerebellum of adolescent male Wistar rats

OBJECTIVE： The present study aimed at coordination and the estimate of the total investigating the effects of curcumin on the motor number of cerebellar Purkinje cells of adolescent Wistar rats exposed to ethanol. METHODS： The total of 21 male Wistar rats aged 37 d old were divided into three groups, namely ethanol, ethanol-curcumin, and control groups. The ethanol group received 1.5 g/kg ethanol injected intraperitoneally and water given per oral; the ethanol-curcumin group received 1.5 g/kg ethanol injected intraperitoneally and curcumin extract given per oral; the control group received saline injection and oral water. The treatment was carried out daily for one month, after which the motor coordination performance of the rats was examined using revolving drum apparatus at test days 1, 8, and 15. The rats were finally sacrificed and the cerebellum of the rats was further processed for stereological analysis. The estimate of the total number of Purkinje cells was calculated using physical fractionator method. RESULTS： The ethanol-curcumin group performed better than both ethanol and control groups in the motor coordination ability at day 8 of testing （P〈 0.01）. No Purkinje cell loss was observed as a result of one month intraperitoneal injection of ethanol. CONCLUSION： Curcumin may exert beneficial effects on the motor coordination of adolescent rats exposed to ethanol via undetermined hormetic mechanisms.

Effects of <i>Pereskia aculeata</i>Miller on the Biochemical Profiles and Body Composition of Wistar Rats

Pereskia aculeata Miller commonly known as “ora-pro-nobis” has been used extensively in folclore medicine. Plant belongs to the family Cactacea and it is found in the Brazil (reaching Bahia and Rio Grande do Sul states). The purpose was to evaluate the effects of plant on biochemical and corporal profiles of Wistar rats. The 80 animals were divided randomly into 4 groups by sex (n = 10 females and 10 males per group) as follows: G1 group (water/rat food ad libitum);G2 group (drink A: commercial condensed milk, sugar and water/rat food ad libitum);G3 group (drink B: P. aculeata juice/rat food ad libitum);G4 group (drink C: commercial condensed milk, sugar and P. aculeate juice/rat food ad libitum). After 35 days their biochemical and corporal profiles were analyzed (cholesterol, glycemia, triglycerides, HDL-c, AST, ALT, Lee Index, weight and visceral fat). Intake of the plant caused no changes in the lipid profile of Wistar rats. However, intake in male rats of the same prevented the increase in triglycerides front of a hypercaloric diet. There were significant changes in glycemic index due to the high carbohydrate content of the plant. The plant was effective in reducing the levels of AST and ALT in male rats and ALT only in females. The use of the plant did not cause significant changes in the index of Lee but the Pereskia aculeata Miller may reduce visceral fat gain in female with a regular diet.

Antiamylase Potential of Telfairia occidentalis Leaves from Cameroon and Effect of Their Dietary Supplementation on Fasting Blood Glucose in Wistar Rats

The study of edible plants, especially in developing countries, might provide more affordable means for the management of diabetes. Telfairia occidentalis is one of the plants whose leaves are commonly consumed in Cameroon. This work hereby studied the antiamylasic potential of its aqueous leaves extract and the effect of its dietary supplementation on fasting blood glucose in Wistar rats. An aqueous extract (1:6) was prepared from shed-dried T. occidentalis leaves by maceration. Its antiamylase activity was evaluated in vitro and a phytochemical screening was realized. Its acute toxicity and its effect on an oral glucose tolerance test (OGTT) were evaluated in rats. The effect of T. occidentalis leaves dietary supplementation (10%) on fasting blood glucose was studied for 28 days in rats fed with carbohydrate enriched diet, using Glibenclamide (0.3 mg/kg body weight) as reference hypoglycemic drug. Results showed that there was total inhibition of α-amylase activity in vitro by T. occidentalis aqueous leaves extract at 0.075 mg/ml. The presence of tannins, flavonoids and anthocyanins was revealed by the phytochemical screening. No sign of toxicity was observed in rats after an oral administration of the extract at 2000 mg/kg body weight. The extract significantly hindered a rise in blood glucose at 400 mg/kg body weight during an oral glucose tolerance test. Dietary supplementation with T. occidentalis leaves caused a significant decrease (p < 0.05) in fasting blood glucose as compared to the positive control. Telfairia occidentalis leaves and their aqueous extract could be used in the management of hyperglycemia and diabetes.

Does Argan Oil Supplementation Affect Metabolic Parameters and Behavior in Wistar Rats?

Argan oil is renowned for its particular biochemical profile: high-fat oleic and linoleic acids, tocopherols, sterols, polyphenols. This composition gives it nutritional, therapeutic and preventive properties against dermatological, metabolic and proliferative diseases. The composition of argan oil assigns its benefits to mental health;it would be provided with possible effects on the prevention and/or cure of stress related disorder. This work aims to evaluate the impact of argan oil dietary on the behavioral response, biochemical and hematological constants and histological profiles of adrenal involved in emotional responses to stress. The variation of these parameters was evaluated in Wistar rats receiving dietary 10 ml/Kg/day of argan oil, starting from weaning, for 13 weeks. Our results show that supplementation has resulted in an increase in locomotor activity, reduced sensitivity to frightening environments with sex dependent variation. Moreover, lipid markers, corticosterone and lymphocytes show a rising trend. If the important role of argan oil diet in cardio-metabolic health is generally well recognized;for mental health, it is the first study that needs further investigation linking between the nervous system, inflammation parameters and metabolism.

Effect of <i>Chromolaena odorata</i>Extracton Hematotoxicity and Spleen Histopathology Induced by <i>Salmonella typhi</i>in Wistar Rats

Salmonella typhi is a facultative intracellular pathogen that causes typhoid fever in humans. In the present study, the effect of Salmonella typhi infection on hematological indices and spleen histology in Wistar rats was investigated and was followed by an evaluation of the ameliorative potential of the methanol leaf extract of Chromolaena odorata (MLECO) compared with ciprofloxacin treatment. The animals were divided into six groups: group 1 was normal control, group 2 was infected with Salmonella typhi without treatment (negative control), groups 3, 4 and 5 were Salmonella typhi infected and treated with 100 mg/kg, 200 mg/kg and 400 mg/kg of the extract respectively and group 6 was also Salmonella typhi infected and treated with 500 mg/70kg of ciprofloxacin. The animals were inoculated with a single infectious dose of Salmonella typhi bacteria and thereafter, treated with the extract and ciprofloxacin for a period of seventeen days, after the animals were confirmed infected. The rats were humanely sacrificed and blood samples taken for haematological investigations, and the spleen harvested and processed for histological examinations. Chromolaena odorata administration reversed the adverse hematotoxicity and histopathological changes in the spleen induced by?Salmonella typhi?infection.

Effect of vitamin A and E on carbohydrate and lipid metabolism in diet-induced obese wistar rats

In the present study, we investigated the biochemical alterations and gene expression of carbohydrate and lipid metabolism after oral administration of vitamin A and E for 2 months in diet-induced obese Wistar rats. Vitamin A and E administration reduced significantly the increase in body weight and food intake and normalized the alterations in lipid profiles in obese rats compared to normal rats. Moreover, both vitamins decreased the fat accumulation in liver tissues of obese rats. Finally, they up-regulated mRNA expression of Pyruvate Kinase (PK) and Glucose Transporter-2 (GLUT-2), and increased lipolysis and cholesterol metabolism through up-regulation of lipoprotein lipase (LPL), Sterol Responsible Element Binding Protein-1a (STREBP-1a) and STREBP-1c mRNA expression. In conclusion, vitamin A and E regulate gene expression of carbohydrate and lipid metabolism, and also ameliorate changes associated with obesity induced by high fat diet in Wistar rats.

Adansonia digitata aqueous leaf extract ameliorates dexamethasone-induced testicular injury in male Wistar rats

Objective:To evaluate the effects of aqueous leaf extract of Adansonia(A.)digitata L on dexamethasone-induced testicular damage in male Wistar rats.Methods:Twenty adult male Wistar rats weighing 170-190 g were divided into four groups.GroupⅠreceived 0.5 mL of phosphate buffer orally for 28 days and served as the normal control group;groupⅡreceived 10 mg/kg of dexamethasone(a synthetic glucocorticoid)intraperitoneally for 7 days and 0.5 mL of phosphate buffer orally for 21 days,groupⅢreceived 10 mg/kg of dexamethasone for 7 days and 800 mg/kg of A.digitata extract orally for 21 days;groupⅣreceived 10 mg/kg of dexamethasone for 7 days and 300 mg/kg of vitamin-E orally for 21 days.Dexamethasone was administered intra-peritoneally for 7 days and all administration lasted for 28 days.The rats were sacrificed by anesthesia with diethyl ether and the testes of each animal were harvested.The testis was homogenized in 0.25 M sucrose at 4℃for biochemical and histological analyses.Results:Administration of dexamethasone significantly decreased body weight,glucose-6-phosphate dehydrogenase(G6PDH),lactate dehydrogenase(LDH),superoxide dismutase(SOD),and glutathione peroxidase(GPx)(P<0.05),and significantly increased malondialdehyde(MDA)activities(P<0.05).The degeneration in the population of spermatogonia and vacuolation and abnormal widening of the interstitial spaces were observed in the rats treated with dexamethasone.However,administration of A.digitata significantly increased SOD,GPx,G6PDH,and LDH levels,significantly decreased MDA activities and improved the histoarchitecture of the testis(P<0.05).Conclusions:A.digitata may have an ameliorative effect on dexamethasone-induced testicular damage in Wistar rats because of its anti-inflammatory and antioxidant properties.

Histological Patterns of Neurodegeneration of Frontal Cortex Neurons in Datura stramonium Treated Wistar Rats

Aim: Datura stramonium (DS) is a known hallucinogen and depressant of the central nervous system, but it is commonly used in alcoholic beverages to increase intoxication. Pharmacological, physiological and ultra-structural studies have demonstrated the neurotoxicity of this drug inanimals and humans at high doses. The present study investigated the histological patterns of neurodegeneration of frontal cortex (FC) neurons in Wistar rats treated with high doses of DS seed extract. Materials and methods: Ethanolic extract of DS dried seeds was diluted in normal saline and administered to male and female Wistar rats weighing 200 g - 250 g. The animals were first placed in three groups which were further sub-divided into four sub-groups. The treated sub-groups received intraperitoneal administration (i.p.) of 750 mg/kg of diluted DS seed extract once daily in group 1, twice daily (1500 mg/kg/day) in group 2 and thrice daily (2250 mg/kg/day) in group 3. The treatment was carried out for 4 weeks while the control groups received normal saline during the same period. The rats were euthanized and sections of the frontal cortices of the brain were histologically processed from all groups. Silver impregnation stain for degenerating axons and neurons was used to elucidate the pattern of degeneration induced by DS seed extract on the neurons of the FC. Results: The results of intraperitoneal administration of DS extract showed no changes in groups 1 & 2 treated rats while group 3 showed a significant pattern of histological changes like axonal atrophy, vacuolization and neuronal deaths in the frontal cortices neurons compared to the controls. Conclusion: DS may have a specific pattern of neurodegeneration at higher doses of administration. This could provide a useful model in understanding how DS intoxication can affect frontal cortex neurons with an implication of neurological disorders, mental diseases and behavioural deficits.

Histological Study of the Therapy in the Essential Oil of Laurusnobilis L. from the West Algerian in Rats Wistar Infected

Laurusnobilis L. is an aromatic plant, widespread in Algeria and widely used by local people as a source of spice for its medicinal properties. The essential oil of this plant native to western Algeria is the subject of the study. The extraction of the essential oil was carded by steam distillation, the yield obtained from the leaves （1.5%）, Wister rats male weight between 100 g and 80 g were infected with Salmonella then treated with anessential oil of Laurusnobilis L.. After sacrifice of the rats, histological examination of the intestines and internal organ （liver and spleen） shows the therapy of this magic plant--＂Laurusnobilis L.＂.

Therapeutic Efficacy of Quinapyramine Sulphate with Freund＇s Complete Adjuvant in Wistar Rats Infected with Trypanosoma Congolense

Therapeutic efficacy of QS （quinapyramine sulphate） and FCA （Freund＇s complete adjuvant） combination was studied. The aim of the study was to evaluate therapeutic efficacy of QS using FCA in Trypanosorna congolense infection. GrouPs treated with QS and FCA had parasite disappeared in peripheral circulation 2 days pi, relapse was observed one week later. Effect of treatment on rectal temperature shows no significance （p 〈 0.05）, normalization of rectal temperature occurred in QS and FCA treated groups （34.1℃） than untreated （42.8 ℃）, QS （37.4 ℃） and FCA （35.92 ℃） treated groups. Mean body weight was significant （p 〈 0.001） in QS and FCA, QS, and FCA groups. Packed cell volume and hemoglobin concentration for untreated groups were lower, but increased in QS, FCA, QS and FCA treated groups, indicating anemia amelioration. White blood cell counted in untreated, QS and FCA treated groups showed no significance （p 〈 0.05）, however, there was leukocytosis due to lymphocytosis in QS and FCA treated group （6.79 × 10^3/μl） compared with untreated and other groups. There was comparative decrease in serum liver enzymes in QS and FCA treated group than other groups. Therefore, QS at lower recommended dose with FCA may enhance efficacy of QS in trypanosomiasis.

Antidiabetic and haematological effect of aqueous extract of stem bark of Afzelia africana(Smith) on streptozotocin-induced diabetic Wistar rats

Objective:To investigate the antidiabetic properties of aqueous extract of stem bark of Afzelia africana(A.africana)and its beneficial effect on haematological parameters in streptozotocin induced diabetic rats.Methods:A total of 30 rats including 24 diabetic and 6 normal rats were used for this study.Diabetes was induced in male Wistar rats by intraperitoneal injection of streptozotocin.After being confirmed diabetic,animals were orally treated with distilled water or extracts at 100 or 200 mg/kg body weight daily for 10 days.The haematological parameters including red blood and white blood cells and their functional indices were evaluated in diabetic treated groups compared with the controls.Results:The extract significantly reduced the blood glucose levels while the best result was obtained at 200 mg/kg body weight The feed and water intake in diabetic rats were significantly reduced while weight loss was minimized at both dosages.Similarly,the levels of red blood,white blood cells and their functional indices were significantly improved after extract administration at both doses.Conclusions:It can be concluded that the aqueous extract of bark of A.africana possesses antihyperglycemic properties.In addition,the extract can prevent various complications of diabetes and improve some haematological parameters.Further experimental investigation is needed to exploit its relevant therapeutic effect to substantiate its ethnomedicinal usage.