The structure of the Rb gene in 32 cases of acute lymphoid leukemia (ALL) were studied by Southern blotting using 32P-labeled Rb cDNA 3. 8 kb probe- Structural abnormalities of Rb gene were found in 8 cases of ALL, an...The structure of the Rb gene in 32 cases of acute lymphoid leukemia (ALL) were studied by Southern blotting using 32P-labeled Rb cDNA 3. 8 kb probe- Structural abnormalities of Rb gene were found in 8 cases of ALL, an incidence of 25%. Two novel fragments (3. 1 kb, 2- 3 kb)were observed in 5 of 8 cases. We used five pairs of Rb gene primers of exons 18, 19, 21, 22, 27 and amplified Rb gene from 6 cases of ALL with abnormal Rb gene- Only one case was free from products of exons 18 and 2l. The results seemed to indicate that abnormalities of Rb gene might be closely associated with initiation and/or promotion of ALL.展开更多
目的 :探讨 AL L 和各亚型中 P16基因失活和纯合缺失的发生率及 m RNA表达。方法 :对 40例AL L (B- AL L2 8例 ,T- AL L7例 ,前 B- AL L5例 )和 15例对照组骨髓标本 ,采用 PCR法检测 P16基因的纯合缺失 ,REP法检测 P16基因的高度甲基化 ...目的 :探讨 AL L 和各亚型中 P16基因失活和纯合缺失的发生率及 m RNA表达。方法 :对 40例AL L (B- AL L2 8例 ,T- AL L7例 ,前 B- AL L5例 )和 15例对照组骨髓标本 ,采用 PCR法检测 P16基因的纯合缺失 ,REP法检测 P16基因的高度甲基化 ,RT- Nest- PCR法检测其 m RNA的表达。结果 :AL L P16基因高度甲基化或纯合缺失发生率为 80 % ,而对照组无一例 P16基因失活 ;T- AL L中 P16基因纯合缺失高于高度甲基化 ;B- AL L中高度甲基化多于纯合缺失。 5例 P16基因高度甲基化尚有 m RNA表达 ,其余均无表达。结论 :P16基因的异常是 AL L 的主要发病机制之一。 T- AL L P16基因的纯合缺失为主要表现 ,而 B- AL L P16的高度甲基化为主要表现。同时 ,无论 P16基因纯合缺失或高度甲基化 ,均能高度抑制 m展开更多
文摘The structure of the Rb gene in 32 cases of acute lymphoid leukemia (ALL) were studied by Southern blotting using 32P-labeled Rb cDNA 3. 8 kb probe- Structural abnormalities of Rb gene were found in 8 cases of ALL, an incidence of 25%. Two novel fragments (3. 1 kb, 2- 3 kb)were observed in 5 of 8 cases. We used five pairs of Rb gene primers of exons 18, 19, 21, 22, 27 and amplified Rb gene from 6 cases of ALL with abnormal Rb gene- Only one case was free from products of exons 18 and 2l. The results seemed to indicate that abnormalities of Rb gene might be closely associated with initiation and/or promotion of ALL.
文摘目的 :探讨 AL L 和各亚型中 P16基因失活和纯合缺失的发生率及 m RNA表达。方法 :对 40例AL L (B- AL L2 8例 ,T- AL L7例 ,前 B- AL L5例 )和 15例对照组骨髓标本 ,采用 PCR法检测 P16基因的纯合缺失 ,REP法检测 P16基因的高度甲基化 ,RT- Nest- PCR法检测其 m RNA的表达。结果 :AL L P16基因高度甲基化或纯合缺失发生率为 80 % ,而对照组无一例 P16基因失活 ;T- AL L中 P16基因纯合缺失高于高度甲基化 ;B- AL L中高度甲基化多于纯合缺失。 5例 P16基因高度甲基化尚有 m RNA表达 ,其余均无表达。结论 :P16基因的异常是 AL L 的主要发病机制之一。 T- AL L P16基因的纯合缺失为主要表现 ,而 B- AL L P16的高度甲基化为主要表现。同时 ,无论 P16基因纯合缺失或高度甲基化 ,均能高度抑制 m