Effect of Panax notoginseng saponins on the expression of beta-amyloid protein in the cortex of the parietal lobe and hippocampus, and spatial learning and memory in a mouse model of senile dementia

BACKGROUND： The pharmacological actions of Panax notoginseng saponins （PNS） lie in removing free radicals, anti-inflammation and anti-oxygenation. It can also improve memory and behavior in rat models of Alzheimer＇s disease. OBJECTIVE： Using the Morris water maze, immunohistochemistry, real-time PCR and RT-PCR, this study aimed to measure improvement in spatial learning, memory, expression of amyloid precursor protein （App） and β -amyloid （A β ）, to investigate the mechanism of action of PNS in the treatment of AD in the senescence accelerated mouse-prone 8 （SAMP8） and compare the effects with huperzine A. DESIGN, TIME AND SETTING： A completely randomized grouping design, controlled animal experiment was performed in the Center for Research ＆ Development of New Drugs, Guangxi Traditional Chinese Medical University from July 2005 to April 2007. MATERIALS： Sixty male SAMP8 mice, aged 3 months, purchased from Tianjin Chinese Traditional Medical University of China, were divided into four groups： PNS high-dosage group, PNS low-dosage group, huperzine A group and control group. PNS was provided by Weihe Pharmaceutical Co., Ltd. （batch No.： Z53021485, Yuxi, Yunan Province, China）. Huperzine A was provided by Zhenyuan Pharmaceutical Co., Ltd. （batch No.： 20040801, Zhejiang, China）. METHODS： The high-dosage group and low-dosage group were treated with 93.50 and 23.38 mg/kg PNS respectively per day and the huperzine A group was treated with 0.038 6 mg/kg huperzine A per day, all by intragastric administration, for 8 consecutive weeks. The same volume of double distilled water was given to the control group. MAIN OUTCOME MEASURES： After drug administration, learning and memory abilities were assessed by place navigation and spatial probe tests. The recording indices consisted of escape latency （time-to-platform）, and the percentage of swimming time spent in each quadrant. The number of A β 1-40, A β 1-42 and App immunopositive neurons in the brains of SAMP8 mice was analyzed by immunohistochemistry. The mRNA content ofApp, tau, acetylcholinesterase, and synaptophysin （Syp） was tested by real time PCR and RT-PCR. RESULTS： The PCR results show that PNS can downregulate the expression of the App gene and upregulate the expression of the Syp gene in the parietal cortex and hippocampus of SAMP8 mice. The therapeutic effects of the PNS high-dosage group were greater than those of the PNS low-dosage group and the huperzine A group （P 〈 0.05）. The results of the Morris water maze and immunohistochemistry indicated that PNS can improve the capacity for spatial learning and memory in SAMP8 mice, and reduce the content of A β 1-40, A β 1-42 and expression of App in the brains of SAMP8 mice. The therapeutic effects of the PNS high-dosage group were greater than that of the PNS low-dosage group and the huperzine A group （P 〈 0.05）. CONCLUSION： These results support the hypothesis that PNS plays a therapeutic and protective role on the pathological lesions and learning dysfunction of Alzheimer＇s disease. The therapeutic effects of PNS for Alzheimer＇s disease are possibly achieved through downregulating the expression of the App gene and upregulating the expression of the Syp gene. The therapeutic effects of PNS are dose-dependent and are greater than the effect of huperzine A.

Two New Dammarane Glycosides from the Acid Hydrolysis Product of Panax Notoginseng

Two new dammarane glycosides named notoginsenoside T-1 and T-2 were isolated from the mild acid hydrolysis products of the root saponins of Panax notoginseng. On the basis of spectroscopic evidences, their structures were elucidated to be 6-O-beta -D-glucopyranosyl-24(25)-epoxy-3 beta ,6 alpha ,12 beta ,23 xi -tetrahydroxydammar-20(22)(E)-ene 1 and 6-O-beta -D-glucopyranosyl-24(25)-epoxy-23 xi -methoxyl-3 beta ,6 alpha ,12 beta -trihydroxydamm-ar-20(22)(E)-ene 2, respectively.

Intervention effect and mechanism of Jisheng Shenqi Decoction plus Panax notoginseng and Bionjia on CCl4-induced hepatic fibrosis rat model

Objective:To investigate the intervention effect and specific mechanism of Jisheng Shenqi Decoction plus Panax notoginseng and Turmeric on carbon tetrachloride(CCl4)-induced liver fibrosis in rats.Methods:SPF SD rats were randomly divided into control group,model group,and Chinese medicine treatment(low,medium and high dose)groups.The model group and Chinese medicine treatment group were given intraperitoneal injection of 40%CCl4 olive oil solution twice a week.A rat model of liver fibrosis was replicated by the method for 8 weeks.After successful modeling,each drug-administered group was gavaged with corresponding drugs,and the control group and model group were gavaged with equal volume of distilled water,once a day,for 4 weeks.After the last intragastric administration,HE staining and Masson staining were used to observe the pathological morphology of liver tissue,and liver function(ALT,AST)was detected;ELISA method was used to determine transforming growth factorβ(TGF-β),angiotensin II(Ang-Ⅱ),chitinase 3-like protein 1(CHI3L1),interleukin-1(IL-1),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)content;The expression levels of type I,type III collagen(Col-Ⅰ,Col-Ⅲ)and TGF-βmRNA were determined by RT-PCR.Results:①HE and Masson staining showed that a large number of liver cells in the model group were inflamed and necrotic,the collagen fibers in the liver tissue were extensively proliferated,the fibrous septa were interconnected,and the pseudolobules were formed.Compared with the model group,the levels of ALT,AST,TGF-β,Ang-Ⅱ,CHI3L1,IL-1,IL-6 and TNF-αin the traditional Chinese medicine treatment group were decreased,while the levels of Col-Ⅰ,Col-ⅢThe expressions ofⅢand TGF-βmRNA were decreased,and the effect was most obvious in the middle and high dose groups of traditional Chinese medicine(P<0.01).Conclusion:Jisheng Shenqi Decoction combined with Panax notoginseng and Biejia can significantly improve liver fibrosis induced by CCl4 in rats,and its mechanism may be related to the down-regulation of angiotensin-converting enzyme(ACE)-angiotensin II(Ang-II)-angiotensin II receptor 1(AT1R)signaling pathway related gene expression,reducing the level of related cytokines,promoting the degradation of extracellular matrix and so on.

超高速液相测定西洋参中人参皂苷Rg_1、Re、Rb_1的含量研究

目的:建立测定西洋参中人参皂苷Rg_1、Re、Rb_1含量的超高速液相色谱法。方法:采用Waters Acquity UPLC BEHC_(18)(100 mm×2.1 mm,1.7μm)柱分离,以甲醇-水为流动相进行梯度洗脱,流速为0.2ml·min^(-1),柱温为35℃,在203 nm处检测。结果:人参皂苷Rg_1、Re、Rb_1在测定范围内有良好的线性关系,其平均回收率为97.36%～99.23%,RSD为1.16%～1.39%(n=6)。结论:本法操作简便,准确度高,重复性好,可作为西洋参质量控制方法之一。

HPLC法同时测定三七总皂苷中人参皂苷Rg_1与Rb_1的含量

目的 建立 HPL C同时测定三七总皂苷中人参皂苷 Rg1 与 Rb1 的方法。方法 采用 HPL C法 ,以茶碱为内标 ,氨基键合相为固定相 ,流动相为乙腈 -水 (80∶ 2 0 ) ,检测波长 2 0 3nm。结果 三七总皂苷中人参皂苷 Rg1 和 Rb1与其他成分分离良好 ,保留时间分别约为 5 .7和 2 1.5 min。人参皂苷 Rg1 在 80～ 2 80μg/ m L (r=0 .9995 ) ,Rb1 在80～ 2 4 0 μg/ m L(r=0 .9993)线性关系良好 ,Rg1 和 Rb1 加样回收率分别为 97.1%和 98.4 %,RSD分别为 2 .4 4 %和 2 .35 %(n=9)。结论 本法操作简便 ,准确 ,重现性好 ,可用于人参及三七的质量控制。

人参皂苷Rb_1在心肌缺血大鼠体内的药动学研究

目的研究人参皂苷Rb_1在正常大鼠和急性心肌缺血模型大鼠体内的药动学。方法以垂体后叶素(PIT)制备急性心肌缺血大鼠模型,实验分3个正常对照组和3个模型组,分别给予高、中、低剂量的人参皂苷Rb_1(400,200,100 mg·kg^(-1)),单次灌胃给药后于不同时点采血,以三七皂苷R_1为内标,采用LC-MS/MS测定各时点血药浓度,并运用PK Solution 2.0软件计算药动学参数;Western Blot法测定大鼠肝脏中药物代谢酶CYP3a2、CYP2c9、CYP2c19、CYP1a1和CYP1a2蛋白表达。结果人参皂苷Rb_1的线性范围为5~10000 ng·mL^(-1),方法的专属性、提取回收率、日间及日内精密度、稳定性符合生物样品处理要求。与正常组比较,模型组大鼠AUC_(0-t)和C_(max)增加,t_(1/2)和T_(max)延长,CL降低(P<0.05或P<0.01)。与正常组比较,模型组大鼠CYP3a2、CYP2c9、CYP2c19、CYP1a1和CYP1a2代谢酶蛋白表达明显下降(P<0.05或P<0.01);人参皂苷Rb_1给药后12 h可诱导CYP1a1和CYP1a2表达。结论该方法专属性强、灵敏度高、准确性好,可用于人参皂苷Rb_1的含量测定及药动学研究。

人参皂甙Rb_1与Re对大鼠缺血再灌注心肌细胞凋亡的影响

目的 观察人参皂甙Rb1与Re对缺血再灌注心肌细胞凋亡的影响 ,并比较两者的效应差异。方法 结扎Wistar大鼠左冠状动脉前降支 ,建立大鼠缺血再灌注动物模型 ;采用透射电镜、缺口末端标记法检测心肌凋亡细胞 ,利用光学显微镜进行细胞计数。结果 (1)透射电镜发现缺血再灌注组缺血区出现心肌凋亡细胞 ,假手术组未发现心肌凋亡细胞 ;(2 )缺血再灌注组心肌细胞凋亡数为 134 45± 45 6 1个 /视野 ,人参皂甙Rb1治疗组 5 1 6 5± 13 71个 /视野 ,人参皂甙Re治疗组 90 6 6± 19 2 2个 /视野 ,三组间有非常显著性差异 (P <0 0 1)。结论 心肌缺血再灌注诱导心肌细胞凋亡 ,人参皂甙Rb1和Re均可显著减少缺血再灌注心肌细胞的凋亡。证实人参皂甙Rb1与Re均有抑制缺血再灌注心肌细胞凋亡 ,减轻心肌缺血再灌注损伤的作用 ;人参皂甙Rb1的抗心肌细胞凋亡作用较Re的效果为佳。

HPLC法测定人参三七颗粒中人参皂苷Rg_1、Re、Rb_1和三七皂苷R_1的含量

目的:建立同时测定人参三七颗粒中人参皂苷Rg_1、Re、Rb_1和三七皂苷R_1含量的方法。方法:采用HPLC法,色谱柱为Sunfire C_(18)(150mm×4.6mm,5μm)分析柱.流动相以乙腈-水梯度洗脱;检测波长为203nm;柱温30℃;流速1.0ml·min^(-1)。结果:人参皂苷Rg_1,Re,Rb_1和三七皂苷R_1之间有较好的分离度,4种成分在线性范围內与峰面积之间线性关系良好,人参皂苷Rg_1、Re、Rb_1和三七皂苷R_1加样回收率分别为99.83%,97.84%,98.43%,97.34%,RSD分别为2.08%,1.66%,1.73%和1.42%(n:5)。结论:本方法可同时测定人参三七颗粒中的人参皂苷Rg_1,Re,Rb_1和三七皂苷R_1含量。

人参皂苷Rb_1对化疗致卵巢早衰大鼠性激素影响

目的探讨人参皂苷皂苷Rb_1对环磷酰胺所致卵巢早衰大鼠性激素的影响。方法选用60只2～3月龄具有正常动情周期雌性Wistar大鼠随机分对照组、给药组和模型组。采用放射免疫法检测卵巢早衰大鼠卵巢促卵泡成熟素(FSH)和雌二醇(E2)水平,同时对比观察人参皂苷皂苷Rb_1治疗后对其性激素水平的影响。结果给药组与模型组的E2浓度差异有统计学意义(P>0.05);给药组的FSH浓度低于模型组(P<0.05)。结论 Rb_1可能是通过上调E2水平、下调FSH水平减少卵巢颗粒细胞的凋亡,进而对化疗所致卵巢早衰起到一定的治疗作用,延缓其衰老。

三七皂甙Rg_1、Rb_1对脂多糖引起的大鼠肝细胞凋亡及相关基因表达的保护作用

目的:探讨三七皂甙对急性肝损害肝细胞凋亡及凋亡相关基因表达的影响。方法:采用脂多糖(LPS)造成大鼠急性肝损伤模型,用 TUNEL 染色法检测肝细胞凋亡,用免疫组化法测定 bcl-2、bax、Fas、FasL 凋亡相关基因在肝内的表达,观察三七皂甙 Rg_1和 Rb_1对上述指标的作用。结果:TUNEL 法检测出细胞凋亡高峰时间在 LPS 处理后12～15 h。免疫组化法检测出 bcl-2、bax、Fas、FasL 凋亡相关基因在肝内表达的时相性变化。Rg_1、Rb_1对 bcl-2没有明显影响,而显著抑制促凋亡基因 bax、Fas、FasL 的表达。结论:LPS处理启动和促进了肝细胞凋亡的相关基因的表达,而 Rg_1、Rb_1通过抑制促凋亡基因表达而减少肝细胞凋亡。

高效液相色谱法测定西洋参冲剂中人参皂甙Rb_1的含量

采用固相萃取净化技术及高效液相色谱法，测定了西洋参冲剂中人参皂甙Rb1的含量，结果在12.5～200μg／ml范围内，Rb1的含量与峰面积呈良好线性，平均回收率及RSD分别为：101．3±3．2％（0．4mg，n＝5）；101．2±2．6％（0．2mg，n＝5）；99．0±4．4％（0．1mg，n＝5）。最小检出量为0．3μg，本法简便、快速、准确。

Notoginseng Saponin Rg1 Prevents Cognitive Impairment through Modulating APP Processing in Aβ1-42-injected Rats

With the intensification of the aging process of the world,Alzheimer's disease(AD),which is the main type of senile dementia,has become a primary problem in the present society.Lots of strategies have been used to prevent and treat AD in animal nlodels and clinical trials,but most of them ended in failure.Panax notoginseng saponins(PNS)contain a variety of monomer compositions which have been separated and identified.Among of the monomer compositions,notoginseng saponin Rg1(Rg1)accounts for 20%of the cultivation of panax notoginseng roots.And now PNS have been reported to be widely used to treat cardicerebrovascular diseases and have neuroprotective effects to restrain theβ-amyloid peptide(Aβ)25-35-niediated apoptosis.Moreover,it is reported that PNS could accelerate the growth of nerve cells,increase the length of axons and promote synaptic plasticity.Whether Rg1 can ameliorate the cognitive impairment and the underlying mechanism has not been elucidated.To study the preventive effect of Rg1 on cognitive impairment and the possible mechanism,we established the cognitive impairment model in rats through Aβ1-42(2.6μg/μL,5μL)injection and then treated the rats with Rg1(25,50 and 100 mg/kg)administered intragastrically for 4 weeks.We observed that Aβ1-42 could induce spatial learning and memory deficits in rats.Simultaneously,Aβ1-42 injection also resulted in the reduced neuron number in comuammonis 1(CA1)and dentate gyrus(DG)of hippocampus,as well as the increased level of hyperphosphorylatedβ-amyloid precursor protein(APP)at Thr668 site with up-regulation ofβ-APP cleaving enzyme 1(BACE1)and presenilin 1(PS1)and down-regulation of a disintegrin and metalloprotease domain-containing protein 10(ADAM 10)and insulindegrading enzyme(IDE).Administration of Rg1 effectively rescued the cognitive impairment and neuronal loss,and inhibited theβ-secretase processing of APP through reducing APP-Thr668 phosphorylation and BACE1/PS1 expression,and increasing the expression of ADAM 10 and IDE.We concluded that Rg1 might have neuroprotective effects and could promote learning and memory ability,which might be a viable candidate in AD therapy probably through reducing the generation of Aβand increasing the degradation of Aβ.

Panax notoginseng saponins promotes cerebral recovery from ischemic injury by downregulating LINGO-1 and activating the EGFR/PI3K/AKT signaling pathways in vivo

Objective:Panax notoginseng saponins (PNS),extracted from rhizome of the herb Radix et Rhizoma Notoginseng (Panax notoginseng (Burk.) F.H.Chen),was recently discovered to have beneficial effects against neurological damage.This study investigated the effects of PNS on cerebral ischemia and elucidated the molecular mechanisms underlying these effects.Methods:Middle cerebral artery occlusion rats were treated with PNS (3.6 mg/100 g or 7.2 mg/100 g per day) for 7 days,the gene of LINGO-1 was measured and the expression of protein synaptophysin,postsynaptic density protein 95,LINGO-1 and p-EGFR/p-PI3K/p-AKT were investigated.The weight and mNSS score of Sprague-Dawley rats in each group were recorded every day during the 7 days.Results:PNS promoted middle cerebral artery occlusion rats' weight and the recovery of neural function.PNS significantly decreased ischemia-induced LINGO-1 protein expression.PNS also elevated EGFR/PI3K/AKT phosphorylation levels.Conclusion:PNS promoted cerebral recovery from ischemic injury by accelerating synapse reconstruction and inhibiting the neuron growth negative regulatory protein LINGO-1 and activating the epidermal growth factor receptor (EGFR)/PI3K/AKT signaling pathway in vivo.

人参皂苷Rb_(1)脂质体的制备及对脂肪细胞中脂滴积聚的抑制作用

[目的]制备β-谷甾醇修饰的人参皂苷Rb_(1)脂质体(β-Rb_(1)-Lip),减少Rb_(1)降解并增强人参皂苷Rb_(1)降脂效果。[方法]采用薄膜水和法制备了β-谷甾醇修饰的人参皂苷Rb_(1)脂质体,利用MTT法评估脂质体的生物安全性,借助油红O染色试验和酶标仪测量TG含量,考察了脂质体β-Rb_(1)-Lip对脂肪细胞3T3-L1的脂滴积聚抑制作用。[结果]制备的β-Rb_(1)-Lip脂质体的包封率为83.74%,平均粒径为198 nm,β-Rb_(1)-Lip在12 h内Rb_(1)释放率约为80%,表现出良好的缓释效果。对于降脂活性,50μmol/L的β-Rb_(1)-Lip表现出显著的细胞内脂滴抑制率,与同浓度Rb_(1)单体相比,β-Rb_(1)-Lip对3T3-L1细胞内脂滴的抑制效果更为显著,且对细胞没有毒副作用。[结论]β-Rb_(1)-Lip具有包封率高、粒径小和缓释效果明显的特征,能够持续释放活性成分人参皂苷Rb_(1),增强其降脂功效并减少用量。

HPLC法测定跌打片中人参皂苷Rg_1和人参皂苷Rb_1的含量

目的:建立高效液相色谱法测定跌打片中人参皂苷Rg★和人参皂苷Rb★的含量测定方法。方法:采用Luna C_(18)色谱柱(250 mm×4.6 mm,5μm);柱温:35℃;流动相为乙腈-水,梯度洗脱;流速1.0 ml·min^(-1);检测波长203 nm。结果:人参皂苷Rg★线性范围为0.340～5.094μg(r=1.000 0),人参皂苷Rb★线性范围为0.303～0.454μg(r=1.000 0);平均加样回收率:人参皂苷Rg_1★为97.17%,RSD=1.09%(n=6),人参皂苷Rb★为102.63%,RSD=1.18%(n=6)。结论:该方法简便可靠,可用于跌打片的质量控制。

HPLC法同时测定腰息痛胶囊中3种活性成分含量

目的 建立HPLC法同时测定腰息痛胶囊中的三七皂苷R_(1)、人参皂苷Rg_(1)、人参皂苷Rb_(1)的含量。方法 采用Omini MP C_(18)(4.6 mm×200 mm,5μm)为色谱柱;流动相为乙腈-0.1%磷酸溶液,梯度洗脱;流速为0.7 ml/min;检测波长为203 nm;柱温为25℃。结果 三七皂苷R_(1)、人参皂苷Rg_(1)、人参皂苷Rb_(1)分别在40.402~1010.500,40.480~1012.000,41.480~1037.000μg范围内呈线性关系(r<0.99);平均加样回收率分别为97.8%,97.5%,98.7%(RSD<2.0%,n=6);精密度、重复性等试验均符合规定。结论 此法简便易行、结果科学,可为中西药复方制剂腰息痛胶囊中的3种皂苷类成分定量控制提供技术依据。

UPLC-MS/MS测定心悦胶囊中6个皂苷类成分的含量

目的:采用超高效液相色谱-质谱法(UPLC-MS/MS)建立心悦胶囊中主要成分人参皂苷Rg_(1)、人参皂苷Re、人参皂苷Rb_(1)、拟人参皂苷F_(11)、人参皂苷Rd和20(S)-人参皂苷F1的含量测定方法。方法:使用Waters ACQUITY BEH C_(18)色谱柱(100 mm×2.1 mm,1.7μm),以乙腈-水为流动相,梯度洗脱,流速为0.5 mL·min^(–1),柱温为40℃,电喷雾电离源,多反应离子监测扫描方式进行检测。结果:6个成分分别在质量浓度为9.638~963.800、9.839~983.900、9.951~995.100、5.270~1054.000、9.608~960.800、4.905~981.000 ng·mL^(–1)时与峰面积线性关系良好(r>0.998),平均加样回收率(RSD)分别为96.9%(2.2%)、100.1%(1.5%)、97.2%(1.8%)、98.5%(2.3%)、96.6%(2.8%)、100.2%(3.5%)。结论:所建立的方法快速、准确、灵敏度高,可用于心悦胶囊中西洋参茎叶总皂苷的质量控制。

UPLC-MS/MS同时测定腰息痛胶囊中5种成分含量

目的建立同时测定腰息痛胶囊中5种成分含量的超高液相色谱-串联质谱法(UPLC-MS/MS)。方法采用Endeavorsil C_(18)(50 mm×2.1 mm,1.8μm)色谱柱;流动相为乙腈(A)-0.1%甲酸(B),梯度洗脱;体积流量0.3 ml/min;柱温30°C;进样量5μl;质谱为电喷雾离子源(ESI源),负离子扫描模式,定量采用多重反应监测模式。结果腰息痛胶囊人参皂苷Rb_(1)、Rb_(3)、Rd、Re和人参皂苷Rg1MS图谱与对照品相同;5种成分在各自范围呈良好线性关系(r>0.9931),平均加样回收率96.7%~97.7%,RSD 0.6%~1.7%,5种成分在5 min内可完全分离。结论该方法高效准确、选择性好,可用于腰息痛胶囊中5种活性成分定量研究。

人参皂苷Rb_(1)和Rg_(1)体外消化产物的UHPLC-TSQ MS分析

本研究采用超高效液相色谱-三重四极杆质谱(ultra-high-performance liquid chromatography-triple quadrupole mass spectrometry,UHPLC-TSQ MS)法分析人参皂苷Rb_(1)和Rg_(1)在模拟唾液、胃液和肠液中的降解产物。在人参皂苷Rb_(1)的消化产物中发现了Rd、Rg_(3)、Rg_(5)、Rk_(1),其中在肠液中还发现了F_(2),表明F_(2)是肠液中特有的降解产物,降解途径为Rb_(1)→Rd→Rg_(3)→Rg_(5)/Rk_(1),Rb_(1)→Rd→F_(2)。在人参皂苷Rg_(1)的消化产物中发现了F_(1)和Rh_(1),降解途径为Rg_(1)→F_(1)和Rg_(1)→Rh_(1)。定量结果表明:降解产物含量在消化液中随时间而变化;在胃液中,Rg_(3)、Rd、Rg_(5)、F_(1)、Rh_(1)和Rk_(1)的达峰时间分别为1、2、4、2、2、4 h;在肠液中,7种降解产物的达峰时间均为4~6 h。另外,皂苷在唾液中的降解较胃液和肠液温和,而在胃肠道中的降解更广泛;在消化过程中,Rg_(1)比Rb_(1)更易降解。人参皂苷在消化道内会发生水解反应生成多种小分子皂苷,为Rb_(1)和Rg_(1)开发和利用提供了重要的化学与生物学基础。

基于网络药理学和分子对接技术探究人参皂苷Rb_(1)抗脑缺血再灌注损伤的作用机制

目的:通过网络药理学和分子对接技术寻找人参皂苷Rb_(1)抗脑缺血再灌注损伤的可能作用机制,为今后实验研究提供一定的理论依据。方法:通过SuperPred数据库获取人参皂苷Rb_(1)相关作用靶点,利用GEO、GeneCards和OMIM数据库获取脑缺血再灌注损伤的异常表达分子,通过交集分析获得人参皂苷Rb_(1)作用于脑缺血再灌注损伤的可能靶点。对所选靶点进行蛋白-蛋白相互作用网络构建、GO功能富集分析和KEGG信号通路富集分析,获得有效靶点的基因和信号通路。最后运用AutoDock软件对关键靶点与人参皂苷Rb_(1)进行分子对接,获得最佳结合靶点。结果:在SuperPred数据库中获得人参皂苷Rb_(1)的潜在作用靶点163个,在GeneCards和OMIM数据库中获得脑缺血再灌注损伤的潜在作用靶点228个,分析后获得14个人参皂苷Rb_(1)与脑缺血再灌注损伤的交集靶点。GO功能富集分析得150个条目,KEGG信号通路富集分析得24条信号通路。分子对接结果显示,人参皂苷Rb_(1)与NFKB1和STAT3有较强的亲和力。结论:人参皂苷Rb_(1)可能通过NFKB1和STAT3信号通路产生抗脑缺血再灌注损伤作用。