Maraviroc promotes recovery from traumatic brain injury in mice by suppression of neuroinflammation and activation of neurotoxic reactive astrocytes

Neuroinflammation and the NACHT,LRR,and PYD domains-containing protein 3 inflammasome play crucial roles in secondary tissue damage following an initial insult in patients with traumatic brain injury(TBI).Maraviroc,a C-C chemokine receptor type 5 antagonist,has been viewed as a new therapeutic strategy for many neuroinflammatory diseases.We studied the effect of maraviroc on TBI-induced neuroinflammation.A moderate-TBI mouse model was subjected to a controlled cortical impact device.Maraviroc or vehicle was injected intraperitoneally 1 hour after TBI and then once per day for 3 consecutive days.Western blot,immunohistochemistry,and TUNEL(terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling)analyses were performed to evaluate the molecular mechanisms of maraviroc at 3 days post-TBI.Our results suggest that maraviroc administration reduced NACHT,LRR,and PYD domains-containing protein 3 inflammasome activation,modulated microglial polarization from M1 to M2,decreased neutrophil and macrophage infiltration,and inhibited the release of inflammatory factors after TBI.Moreover,maraviroc treatment decreased the activation of neurotoxic reactive astrocytes,which,in turn,exacerbated neuronal cell death.Additionally,we confirmed the neuroprotective effect of maraviroc using the modified neurological severity score,rotarod test,Morris water maze test,and lesion volume measurements.In summary,our findings indicate that maraviroc might be a desirable pharmacotherapeutic strategy for TBI,and C-C chemokine receptor type 5 might be a promising pharmacotherapeutic target to improve recovery after TBI.

Biodecolorization and partial mineralization of Reactive Black 5 by a strain of Rhodopseudomonas palustris

A strain of photosynthetic bacterium, Rhodopseudomonas palustris W1, isolated from a lab-scale anaerobic moving bed biofilm reactor (MBBR) treating textile e?uent was demonstrated to decolorize Reactive Black 5 (RB5) effciently under anaerobic condition. By a series of batch tests, the suitable conditions for RB5 decolorization were obtained, namely, pH < 10, light presence, glutamine or lactate as carbon source with concentration more than 500 mg/L when lactate is selected, NH4Cl as a nitrogen source wi...

Effects of glucose on the decolorization of Reactive Black 5 by yeast isolates

The cometabolic roles of glucose were investigated in decolorization of an azo dye, Reactive Black 5, by yeast isolates, Debaryomyces polymorphus and Candida tropicalis. The results indicated that the dye degradation by the two yeasts was highly associated with the yeast growth process and glucose presence in the medium. Color removal of 200 mg dye/L was increased from 76.4% to 92.7% within 60 h to 100% within 18-24 h with the increase of glucose from 5 to 10 g/L, although the activity of manganese dependent peroxidase （MnP） decreased by 2-8 times in this case. Hydrogen peroxide of 233.3 μg/L was detected in 6 h in D. polymorphus culture. The cometabolic functions of glucose and hydrogen peroxide could be also confirmed by the further color removals of 95.8% or 78,9% in the second cycle of decolorization tests in which 7 g glucose/L or 250 μg H202/L was superadded respectively together with 200 mg dye/L.

6-甲基-5-庚烯-2-酮诱导砀山酥梨虎皮病发生与活性氧代谢的关系

探究6-甲基-5-庚烯-2-酮(6-methyl-5-hepten-2-one,MHO)处理对砀山酥梨虎皮病关系及对活性氧代谢影响。测定MHO处理砀山酥梨果皮在冷藏过程中α-法尼烯、共轭三烯、MHO、丙二醛、过氧化氢(H_(2)O_(2))、超氧阴离子自由基、总酚含量及过氧化氢酶(catalase,CAT)、过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)和多酚氧化酶(polyphenol oxidase,PPO)的活性,并观察和统计虎皮病的发病情况。结果表明,外源MHO可以诱发相似虎皮病的症状,并且显著增加果皮中MHO、H_(2)O_(2)和超氧阴离子自由基的含量,降低抗氧化酶CAT、POD和SOD的活性,增加了α-法尼烯、共轭三烯的含量。果皮MHO含量与H_(2)O_(2)和超氧阴离子自由基含量呈极显著相关,但果皮的MHO含量比H_(2)O_(2)与虎皮病发病率的关系更密切,这些结果表明MHO可能通过增加活性氧的积累而引发梨虎皮病。

Development of the N-Doped Cu-Carbon Composite as a Novel Catalyst for the Removal Reactive Black 5

In this study, two Cu-based catalysts with and without N doped carbon matrix, named N-Cu/CuO/C and Cu/CuO were synthesized via calcination of melamine-cupper acetate complex and cupper acetate at 500°C under an inert atmosphere. The catalysts were characterized by X-ray powder diffraction (XRD), Field Emission Scanning Electron Microscope (FESEM), and CHNS elemental analyzer. The catalytic activity of both catalysts was evaluated through the NaBH4 associated reduction of commercial textile dye named reactive black 5 (RB5). The kinetics of the reduction of reactive black 5 was also described by the pseudo-first-order kinetic equation. For the studied reduction, N-Cu/CuO/C exhibited enhanced catalytic activity both in conversion and kinetics (97% conv. in 315 sec) compared to that of by Cu/CuO/C (25% conv. in 1500 sec). Besides, N-Cu/CuO/C also demonstrated good reusability up to four consecutive cycles.

TiO₂PC500 Coated on Non Woven Paper with SiO₂as a Binder-Assisted Photocatalytic Degradation of Reactive Black 5 in Aqueous Solution

Photocatalytic discoloration kinetics of Reactive Black 5 (RB5), a vinylsulfone dye, has been studied spectrophotometrically by following the decrease in dye concentration with time at ambient conditions using a flow loop reactor. UV lump, Black Light Blue (BLB) emitting at maximum wavelength of 365 nm and Ahlstrom Research Service paper consistent of TiO2 P500 coated on non woven paper was used respectively as source of UV light and photocatalyst. At natural pH, the result shows that photolysis of RB5 and its adsorption in the presence of photocatalyst was negligible while the photocatalytic oxidation (PCO) permits 30.8% of RB5 degradation. The degradation of dye was studied under a variety of conditions such as volumetric flow rate, initial pH, photocatalyst reuse, and in the presence of electron acceptor such as sodium persulphate ((Na)2S2O8). The degradation rates were found to be strongly influenced by all the above parameters. The circulation flow rate of 108 L/h was the best. The rate constant calculated when the initial pH was varied shows that pH 3 was more favorable for RB5 removal. Peroxydisulphate ions have the strong effect on RB5 discoloration even in dark without and with photocatalyst. When UV light was used in the presence of photocatalyst, 50 min was enough for quasi-total removal of RB5 with (0.2 M).

Biotransformation of Carmoisine and Reactive Black 5 Dyes Using <i>Saccharomyces cerevisiae</i>

Saccharomyces cerevisiae (baker’s yeast) is the most important industrial microorganisms. This yeast is commonly used as a leavening agent in baking bread and bakery products, where it produces carbon dioxide from converting of the fermentable sugars present in the dough. Nowadays, industrial and chemical activities led to produce new compounds with new kinds of contamination in the environment. Discharge of untreated or partially treated industrial sewage has created the contamination problems of rivers and lakes such as drugs, oil, heavy metals, paints, pesticides and various chemical compounds in them. Hence, it is necessary to control and reduce the levels of these compounds in wastewater and bring them to permissible values. This study aims to study the bioconversion potential of commonly available Saccharomyces cerevisiae for the two textile dyes of Carmoisine and Reactive Black 5. Reaction mixtures for biotransformation of dyes included 50 mg/l Carmoisine or 25 mg/l Reactive Black 5 and 1% dried harvested cells of S. cerevisiae (bread’s yeast) were tested. Harvested dry and wet yeast were studied for this purpose. The results show that harvested cells of Saccharomyces cerevisiae are able to bioconvert Carmoisine and Reactive Black 5. Reactive Black 5, Carmoisine are degraded by biotransformation 85% and 53% within 24 hours in water at the room temperature.

Multiwall Carbon Nanotube Modified Electrochemical Sensor for Reactive Black 5

Cyclic voltammograms of reactive black5 (RB5) at different pHs in the range 1.0 - 13.0 on multiwall carbon nanotube modified glassy carbon electrode revealed the presence of one well-defined irreversible anodic peak around 975 mV in acidic and neutral pHs. Adsorption controlled oxidation observed at acidic pH 1.0 resulted in the maximum peak current response in cyclic voltammograms. A systematic differential pulse stripping voltammetric studies were carried out using the modified electrode at pH 1.0. The accumulation parameters, accumulation potential and time were optimized for maximum adsorption of the dye which was ascertained from the SEM photographs and XRD results. The stripping parameters were optimized and calibration was made under optimum conditions. The range of study was from 0.5 ppm to 100 ppm and the lower limit of determination was 100 ppm. Five identical experiments were carried out and the RSD value obtained was 2.5% suggesting good reproducibility. The proposed method was successfully applied to determine the concentration of dye in the fabric and wastewater after dyeing.

ICU急性呼吸衰竭患者外周血HCAR、DcR3、AQP-5水平与机械通气撤机结局的关系及价值分析

目的 探究重症监护室(ICU)急性呼吸衰竭患者外周血超敏C反应蛋白与白蛋白比值(HCAR)、诱骗受体3(DcR3)、水通道蛋白-5(AQP-5)水平与机械通气撤机结局的关系及临床价值。方法 选取连云港市第二人民医院2018年8月—2021年8月ICU急性呼吸衰竭患者120例,均行机械通气治疗,在符合自主呼吸试验(SBT)指征且通过30 min SBT后撤机,根据撤机后48 h内是否再插管分为撤机成功组(87例)和撤机失败组(33例),撤机前采集外周血检测HCAR、DcR3、AQP-5水平,分析外周血HCAR、DcR3、AQP-5水平与撤机结局的关系及预测价值。结果 两组呼吸衰竭类型、合并器官功能障碍综合征(MODS)、肺部超声评分(LUS)、急性生理与慢性健康评价系统Ⅱ(APACHEⅡ)评分差异有统计学意义(P<0.05);撤机失败组外周血HCAR、DcR3高于撤机成功组,AQP-5低于撤机成功组(P<0.05);Pearson相关性分析显示外周血HCAR、DcR3与LUS、APACHEⅡ评分呈正相关,AQP-5与LUS、APACHEⅡ评分呈负相关(P<0.05);单因素、多因素分析均显示,外周血HCAR、DcR3、AQP-5影响撤机结局(P<0.05);外周血HCAR、DcR3、AQP-5预测撤机失败的截断值分别为4.10 mg/g、14.55μg/L、7.91μg/L,联合预测撤机失败的曲线下面积(AUC)为0.915(95%CI:0.850~0.958),大于各指标单独预测;以截断值为界分为低水平与高水平,外周血HCAR、DcR3高水平患者30 d生存率低于低水平患者,外周血AQP-5高水平患者30 d生存率高于低水平患者(P<0.05)。结论 ICU急性呼吸衰竭患者外周血HCAR、DcR3、AQP-5水平与撤机结局密切相关,联合检测可作为预测撤机失败的重要辅助手段,还能帮助临床判断死亡风险,为临床提供可靠的数据支持。

Rational design of Aspergillus flavus A5p1-immobilized cell system to enhance the decolorization of reactive blue 4(RB4)

Anthraquinone dyes are a class of typical carcinogenic and hard-biodegradable organic pollutants.This study aimed to enhance the decolorization of anthraquinone dye by rationally designing an expected immobilized system.Reactive blue 4(RB4) was used as a substrate model and a previous isolated dyedegrading strain Aspergillus flavus A5pl was purposefully immobilized.Considering the effects of cell attachment and mass transfer,the polyurethane foam(PUF) with open pore structure was selected as the immobilization carrier.Results showed that the RB4 decolorization efficiency was significant enhanced after immobilization.Compared to the free mycelium system,the decolorization time of200 mg·L^(-1)RB4 was shortened from 48 h to 28 h by the PUF-immobilized cell system.Moreover,the PUF-immobilized system could tolerate RB4 up to 2000 mg-L^(-1).In the packed bed bioreactor(PBBR),an average decolorization efficiency of 93.3% could be maintained by the PUF-immobilized system for26 days.The decolorization process of RB4 was well described by the logistic equation and the degradation pathway was discussed.It was found that the higher specific growth rate of the PUF-immobilized cells was one of reasons for the enhanced decolorization.The good performance of the PUFimmobilized cell system would make it have potential application value for RB4 bioremediation.

OsATL32 ubiquitinates the reactive oxygen species-producing OsRac5-OsRbohB module to suppress rice immunity

Ubiquitination-mediated protein degradation is integral to plant immunity,with E3 ubiquitin ligases acting as key factors in this process.Here,we report the functions of OsATL32,a plasma membrane-localized Arabidopsis Tóxicos En Levadura(ATL)-type E3 ubiquitin ligase,in rice(Oryza sativa)immunity and its associated regulatory network.We found that the expression of OsATL32 is downregulated in both compatible and incompatible interactions between rice and the rice blast fungus Magnaporthe oryzae.The OsATL32 protein level declines in response to infection by a compatible M.oryzae strain or to chitin treatment.OsATL32 negatively regulates rice resistance to blast and bacterial leaf blight diseases,as well as chitin-triggered immunity.Biochemical and genetic studies revealed that OsATL32 suppresses pathogen-induced reactive oxygen species(ROS)accumulation by mediating ubiquitination and degradation of the ROS-producing OsRac5–OsRbohB module,which enhances rice immunity against M.oryzae.The protein phosphatase PHOSPHATASE AND TENSIN HOMOLOG enhances rice blast resistance by dephosphorylating OsATL32 and promoting its degradation,preventing its negative effect on rice immunity.This study provides insights into the molecular mechanism by which the E3 ligase OsATL32 targets a ROS-producing module to undermine rice immunity.

LncRNA NKILA suppresses airway hyper reactivity via interfering the facilitation of MUC5AC and MUC5B mediated by GALNT2

Glycosylation of mucins mediated by N-acetylgalactosaminyltransferases(GALNTs)is closely related to respiratory diseases such as asthma and chronic obstructive pulmonary disease(COPD).In addition,long non-coding RNAs(LncRNAs)participate in physiological and pathological processes through various epigenetic mechanisms.In this study,we found that a novel LncRNA named NKILA combined with multiple mucins and GALNTs potentially by several bioinformatics methods,and we used quantitative real-time PCR(RT-qPCR)to detect the expressions of NKILA,MUC5AC,MUC5B,and GALNT2 mRNA in 50 cases of asthma samples and 19 cases of normal samples,whose results showed that the expression of NKILA was significantly decreased in asthmatic samples,negatively correlated with the severity of asthma and the expressions of MUC5AC and MUC5B,while GALNT2 was significantly increased in asthmatic tissues,and positively correlated with the severity of asthma and the expressions of MUC5AC and MUC5B.In vitro,we used transient transfection technology to overexpress or interfere with NKILA and GALNT2 and then detected the expressions of MUC5AC and MUC5B via RT-qPCR and Western blot,which demonstrated GALNT2 can promote the expressions of MUC5AC and MUC5B protein,while NKILA could inhibit this effect.Furthermore,co-immunoprecipitation results showed that GALNT2 could bind to MUC5AC and MUC5B protein.RNA immunoprecipitation and RNA pull-down experiments showed that NKILA could bind to GALNT2.These evidences suggested that there are correlations among the expression of NKILA,GALNT2,MUC5AC,and MUC5B proteins in asthmatic patients.Mechanically,we concluded that NKILA can suppress the O-linked glycosylation of MUC5AC and MUC5B proteins by binding to GALNT2 and inhibit the expression of MUC5AC and MUC5B proteins.Our researches provided a potential therapeutic target for AHR.

污泥基生物炭制备及其对活性黑5染料的吸附研究

以污水处理厂剩余污泥为原料制备生物炭是富有潜力的剩余污泥资源化途径。利用剩余污泥制备了生物炭,将其用于处理废水中的有机染料活性黑5,通过调整制备工艺参数,考察了粒径、反应温度和投加量对污泥基生物炭吸附性能的影响,并对其结构和形貌进行了研究,结果表明:原料污泥粒径、反应温度对污泥基生物炭吸附性能均有明显影响,最佳制备工艺条件为采用0.074 nm(200目)粒径污泥颗粒、反应温度450℃经马弗炉焚烧制取;污泥基生物炭投加量为7 g/L时,对50 mg/L模拟废水中活性黑5染料的去除率可达到79.66%,吸附量为5.7 mg/g,对100 mg/L模拟废水中活性黑5染料的去除率可达到68.76%,吸附量为9.8 mg/g。

糖尿病兔心房5脂氧合酶及12脂氧合酶蛋白变化研究

目的观察糖尿病兔心房炎症和心房纤维化改变及5脂氧合酶(ALOX5)、12脂氧合酶(ALOX12)、单核细胞趋化蛋白1(MCP-1)在心房组织中表达变化。方法选择健康雄性日本大耳白兔13只,随机选取8只白兔建立1型糖尿病兔模型,其中5只建模成功作为糖尿病组,另外5只白兔作为对照组。检查超声心动图、病理指标,并提取左心房组织蛋白进行Western blot检测。结果与对照组比较,糖尿病组室间隔厚度明显增加,LVEF明显降低,差异有统计学意义(P<0.05)。糖尿病组左心房组织胶原容积分数较对照组明显增加[(7.97±1.01)%vs(3.11±0.99)%,P<0.01]。糖尿病组左心房组织ALOX5、ALOX12表达明显高于对照组,差异有统计学意义(P<0.05);2组MCP-1表达比较,差异无统计学意义(P>0.05)。结论ALOX5、ALOX12表达可能与糖尿病兔心房炎症及纤维化相关。

Fabrication of Ti-Ni-Mo-Si composite coating by reactive braze coating process

Ti-Ni-Mo-Si composite coating was fabricated on mild steel by reactive braze coating process with Ti61. 9Ni24. 6Si4. 411409.1 （ wt. % ） powders as the raw materials. Microstr^cture of the coating was characterized by optical microscopy, scanning electron microscopy, X-ray diffraction and energy dispersive spectroscopy and micro-hardness tester. Results indicate that the Ti-Ni-Mo-Si composite coating is metallurgically bonded to the mild steel substrate and has high hardness. The microstructure of the coating consists of the reinforcement of Ti5 Si3 and Mo9 Ti4 particles and the matrix of eutectic NiTi2. Due to the poor wettability of NiTi2 liquid at low temperature, TisSi3 and Mo9 Ti4 do not uniformly distribute in the NiTi2 matrix.

A novel derivative of Genistein inhibits proliferation of ovarian cancer HO-8910 cells by regulating reactive oxygen species

Objective To investigate the anticancer effect of a novel derivative of genistein(5-hydroxy-4’-nitro-7-propionyloxy-genistein,HNPG)on human ovarian cancer HO-8910 cells and its possible molecular mechanism.Methods HO-8910 cells were cultured in vitro,and the inhibitory effect of HNPG on proliferation was determined using MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]assay.The effect of HNPG on inducing apoptosis was examined using FCM with Annexin V-FITC and propidium iodide staining.The effect of HNPG on regulating reactive oxygen species(ROS)was measured using FCM with 2’,7’-di chlorodihydro-fluorescein diacetate staining.The effect of HNPG on modulating mitochondrial membrane potential(MMP)was determined using FCM with lipophilic cationic dye 2(6 Amino 3 imino 3H xanthen 9 yl)benzoic acid methyl ester(Rh123)staining.The bioactivity of superoxide dismutase(SOD)and catalase(CAT)and the content of glutathione(GSH)and malondialdehyde(MDA)were detected using enzymelinked immunosorbent assay.The related apoptotic proteins,including bcl-2,bax,cyt-c,and cleavedcaspase-3,were assessed using western blotting.Results HNPG exhibited dramatic antitumor activity against HO-8910 cells in vitro,inhibited proliferation,and induced apoptosis in a time-and dose-dependent manner.These effects were accompanied by reduced bioactivity of SOD and CAT,reduced GSH content,and enhanced MDA content.Simultaneously,the amount of ROS was increased and the level of MMP was reduced,along with upregulation of mitochondrial apoptosis pathway-related proteins,bax,cyt-c,and cleaved-caspase-3;bcl-2 protein was downregulated.Conclusion HNPG inhibited proliferation of human ovarian cancer HO-8910 cells in vitro,which might be related to decreased bioactivity of SOD and CAT.HNPG also reduced GSH content,which resulted in ROS accumulation in cells,damaged the integrity of mitochondrial membrane,and induced cell apoptosis.

好氧颗粒污泥对活性黑5染料的降解

为探究活性黑5染料在好氧颗粒污泥系统中的去除效果和降解机理,在等工作体积的序批式反应器(sequencing batch reactor,SBR)中,采用厌氧好氧交替环境培养好氧颗粒污泥处理模拟偶氮染料废水,测定SBR系统对COD、NH_(4)^(+)-N、PO_(4)^(3-)-P的去除效率及对活性黑5的去除负荷,并通过16S rRNA基因高通量测序分析偶氮染料的去除机理。结果表明:SBR系统对污染物去除效果良好,培养末期对COD、NH_(4)^(+)-N、PO_(4)^(3-)-P的去除率分别可达90%、95%、95%以上,对活性黑5的去除负荷稳定在约0.005 kg·(kg·d)^(-1)。活性黑5的偶氮键断裂主要发生在厌氧阶段,产生的含苯环和萘环的中间产物主要在好氧阶段通过氧化去除。16S rRNA基因高通量测序结果显示,偶氮染料提高了SBR系统硝化菌门以及具有反硝化或降解芳香类物质功能的Paracoccus、Azoarcus和Thauera属的丰度。

Li_(3)Mg_(2)SbO_(5)F_(2)陶瓷微波介电性能研究

采用反应烧结法制备Li_(3)Mg_(2)SbO_(5)F_(2)微波介质陶瓷,并研究烧结温度等条件对其相组成、显微组织和微波介电性能的影响。采用X射线衍射仪、扫描电子显微镜和矢量网络分析仪对所制备Li_(3)Mg_(2)SbO_(5)F_(2)微波介质陶瓷的结构和微波介电性能进行表征。实验结果表明,825℃烧结的Li_(3)Mg_(2)SbO_(5)F_(2)陶瓷发生了部分立方-正交结构相变。Li_(3)Mg_(2)SbO_(5)F_(2)陶瓷的介电常数主要受体密度的影响,而其品质因数受密度和结构相变共同影响。800℃/5 h烧结的Li_(3)Mg_(2)SbO_(5)F_(2)陶瓷具有良好微波介电性能,其介电常数为7.9,品质因数为58 600 GHz,谐振频率温度系数为-53 ppm/℃。

轻烃芳构化的系统研究(Ⅱ)——ZnNi/HZSM-5催化剂的再生工艺考察

研究了轻烃芳构化 Ｚｎ Ｎｉ／ Ｈ Ｚ Ｓ Ｍ － ５ 催化剂的失 活类型， 认为积炭失活是改性 Ｚ Ｓ Ｍ － ５ 催化剂的最主要失活方式，经烧焦可恢复其催化活性。通过马弗炉 静态烧焦和反应管动态烧 焦， 考察了再生温度对烧焦过程的影响，推测出再生模式。在小型连续式１００ ｍ Ｌ 固定床装置上在宏观热效应方面模拟了工业烧焦再生过程，认为烧焦是一个从外向内逐步扩散的过程，在催化剂上会形成热点，在烧焦再生的影响因素（ 再生气的氧含量、空速，再生最高温度和时间等） 中，再 生最高温度的影响是最显 著的， 只有达到５７５ ℃才能恢复催化剂活性；而再生气空速和氧含量决定了再生时间。在所考察的３ 种再生方案中，确定了最佳的再生方案（ 低起燃温度，一次烧焦） 。该方案不仅比以往 方案省时５０ ％ ，而且 再生后 Ｚｎ Ｎｉ／ Ｈ Ｚ Ｓ Ｍ－ ５ 催化性能恢复好，稳定性不变。

高效混合菌群FF的筛选及其对活性黑5的脱色作用

利用梯度浓度压力驯化法,从运行良好的印染废水生物处理系统(A+OSA剩余污泥减量系统)二号厌氧反应器活性污泥中,筛选驯化出对活性黑5具有良好脱色性能的混合菌群FF,针对其染料脱色性能进行了一系列研究.结果表明:混合菌群FF在活性黑5初始质量浓度为200mg/L的脱色培养基中,在35℃、pH值为8.0的条件下,静置培养12h,测定其平均脱色率为84.6%,24h后其最高脱色率可达91.4%;混合菌群FF具有一定的耐盐能力,在NaCl质量分数为4%时,24h后脱色率仍保持在91.0%;同时混合菌群FF对活性蓝19、活性艳橙X-GN、活性红239等多种小分子活性染料具有较好脱色性能,静置培养24h时,脱色率都达到了90.0%以上,说明混合菌群FF对偶氮类活性染料脱色具有一定的普适性.利用液相色谱-质谱联用仪(LC-MS)测定混合菌群作用过程中活性黑5的脱色降解中间产物,初步推测了其脱色降解机理.同时利用纯培养16SrDNA鉴定技术对混合菌群FF的群落结构进行解析,发现混合菌群FF中的功能优势菌群主要为变形菌纲(Gamma-proteobacteria)的克雷伯氏菌属(Klebsiella)细菌和变形杆菌属(Proteus)细菌.