Objective:To investigate the involvement of reactive oxygen species detoxification system in Anopheles stephensi during Plasmodium berghei midgut invasion.Methods:Eight key reactive oxygen species metabolizing enzymes...Objective:To investigate the involvement of reactive oxygen species detoxification system in Anopheles stephensi during Plasmodium berghei midgut invasion.Methods:Eight key reactive oxygen species metabolizing enzymes were cloned and characterized,and their expression was monitored in parasite-infected mosquitoes.Results:Superoxide anion detoxifying superoxide dismutases(Fe/Mn SOD,Cu/Zn SOD 2,Cu/Zn SOD 3A,and Cu/Zn SOD 3B)depicted varied expression patterns.Fe/Mn SOD expression declined,whereas Cu/Zn SOD expression was elevated in the infected mosquitoes.Peroxidases,catalase and glutathione peroxidase showed lack of induction in expression during the Plasmodium berghei infection.Further,expression of thioredoxin reductase increased in the infected mosquitoes,whereas gluthathione S-transferase levels decreased markedly.Conclusions:Detoxification enzymes may play a role in modulating host immunity and parasite transmission.展开更多
There is compelling evidence to substantiate the notion that oxidative stress,in which the production of reactive oxygen species(ROS)overwhelms antioxidant defense,is a common feature of many acute and chronic disorde...There is compelling evidence to substantiate the notion that oxidative stress,in which the production of reactive oxygen species(ROS)overwhelms antioxidant defense,is a common feature of many acute and chronic disorders.ROS,the antioxidant system,and multiple redox factors underlie the pivotal roles of acupuncture on oxidative stress.Given the complexity of action mechanisms of acupuncture on oxidative stress,additional studies are needed to identify the most crucial mechanism that is applicable not only in animal models but also in patients suffering from oxidative damage.展开更多
文摘Objective:To investigate the involvement of reactive oxygen species detoxification system in Anopheles stephensi during Plasmodium berghei midgut invasion.Methods:Eight key reactive oxygen species metabolizing enzymes were cloned and characterized,and their expression was monitored in parasite-infected mosquitoes.Results:Superoxide anion detoxifying superoxide dismutases(Fe/Mn SOD,Cu/Zn SOD 2,Cu/Zn SOD 3A,and Cu/Zn SOD 3B)depicted varied expression patterns.Fe/Mn SOD expression declined,whereas Cu/Zn SOD expression was elevated in the infected mosquitoes.Peroxidases,catalase and glutathione peroxidase showed lack of induction in expression during the Plasmodium berghei infection.Further,expression of thioredoxin reductase increased in the infected mosquitoes,whereas gluthathione S-transferase levels decreased markedly.Conclusions:Detoxification enzymes may play a role in modulating host immunity and parasite transmission.
基金supported by the National Natural Science Foundation of China(Grant Nos.30801483, 81222050,81303122)the Beijing Nova Program (Grant No.2014B063)
文摘There is compelling evidence to substantiate the notion that oxidative stress,in which the production of reactive oxygen species(ROS)overwhelms antioxidant defense,is a common feature of many acute and chronic disorders.ROS,the antioxidant system,and multiple redox factors underlie the pivotal roles of acupuncture on oxidative stress.Given the complexity of action mechanisms of acupuncture on oxidative stress,additional studies are needed to identify the most crucial mechanism that is applicable not only in animal models but also in patients suffering from oxidative damage.
