High mobility group box 1 in the central nervous system:regeneration hidden beneath inflammation

High-mobility group box 1 was first discovered in the calf thymus as a DNA-binding nuclear protein and has been widely studied in diverse fields,including neurology and neuroscience.High-mobility group box 1 in the extracellular space functions as a pro-inflammatory damage-associated molecular pattern,which has been proven to play an important role in a wide variety of central nervous system disorders such as ischemic stroke,Alzheimer’s disease,frontotemporal dementia,Parkinson’s disease,multiple sclerosis,epilepsy,and traumatic brain injury.Several drugs that inhibit high-mobility group box 1 as a damage-associated molecular pattern,such as glycyrrhizin,ethyl pyruvate,and neutralizing anti-high-mobility group box 1 antibodies,are commonly used to target high-mobility group box 1 activity in central nervous system disorders.Although it is commonly known for its detrimental inflammatory effect,high-mobility group box 1 has also been shown to have beneficial pro-regenerative roles in central nervous system disorders.In this narrative review,we provide a brief summary of the history of high-mobility group box 1 research and its characterization as a damage-associated molecular pattern,its downstream receptors,and intracellular signaling pathways,how high-mobility group box 1 exerts the repair-favoring roles in general and in the central nervous system,and clues on how to differentiate the pro-regenerative from the pro-inflammatory role.Research targeting high-mobility group box 1 in the central nervous system may benefit from differentiating between the two functions rather than overall suppression of high-mobility group box 1.

Real-Time Monitoring Method for Cow Rumination Behavior Based on Edge Computing and Improved MobileNet v3

[Objective]Real-time monitoring of cow ruminant behavior is of paramount importance for promptly obtaining relevant information about cow health and predicting cow diseases.Currently,various strategies have been proposed for monitoring cow ruminant behavior,including video surveillance,sound recognition,and sensor monitoring methods.How‐ever,the application of edge device gives rise to the issue of inadequate real-time performance.To reduce the volume of data transmission and cloud computing workload while achieving real-time monitoring of dairy cow rumination behavior,a real-time monitoring method was proposed for cow ruminant behavior based on edge computing.[Methods]Autono‐mously designed edge devices were utilized to collect and process six-axis acceleration signals from cows in real-time.Based on these six-axis data,two distinct strategies,federated edge intelligence and split edge intelligence,were investigat‐ed for the real-time recognition of cow ruminant behavior.Focused on the real-time recognition method for cow ruminant behavior leveraging federated edge intelligence,the CA-MobileNet v3 network was proposed by enhancing the MobileNet v3 network with a collaborative attention mechanism.Additionally,a federated edge intelligence model was designed uti‐lizing the CA-MobileNet v3 network and the FedAvg federated aggregation algorithm.In the study on split edge intelli‐gence,a split edge intelligence model named MobileNet-LSTM was designed by integrating the MobileNet v3 network with a fusion collaborative attention mechanism and the Bi-LSTM network.[Results and Discussions]Through compara‐tive experiments with MobileNet v3 and MobileNet-LSTM,the federated edge intelligence model based on CA-Mo‐bileNet v3 achieved an average Precision rate,Recall rate,F1-Score,Specificity,and Accuracy of 97.1%,97.9%,97.5%,98.3%,and 98.2%,respectively,yielding the best recognition performance.[Conclusions]It is provided a real-time and effective method for monitoring cow ruminant behavior,and the proposed federated edge intelligence model can be ap‐plied in practical settings.

Real-Time Prediction of Urban Traffic Problems Based on Artificial Intelligence-Enhanced Mobile Ad Hoc Networks(MANETS)

Traffic in today’s cities is a serious problem that increases travel times,negatively affects the environment,and drains financial resources.This study presents an Artificial Intelligence(AI)augmentedMobile Ad Hoc Networks(MANETs)based real-time prediction paradigm for urban traffic challenges.MANETs are wireless networks that are based on mobile devices and may self-organize.The distributed nature of MANETs and the power of AI approaches are leveraged in this framework to provide reliable and timely traffic congestion forecasts.This study suggests a unique Chaotic Spatial Fuzzy Polynomial Neural Network(CSFPNN)technique to assess real-time data acquired from various sources within theMANETs.The framework uses the proposed approach to learn from the data and create predictionmodels to detect possible traffic problems and their severity in real time.Real-time traffic prediction allows for proactive actions like resource allocation,dynamic route advice,and traffic signal optimization to reduce congestion.The framework supports effective decision-making,decreases travel time,lowers fuel use,and enhances overall urban mobility by giving timely information to pedestrians,drivers,and urban planners.Extensive simulations and real-world datasets are used to test the proposed framework’s prediction accuracy,responsiveness,and scalability.Experimental results show that the suggested framework successfully anticipates urban traffic issues in real-time,enables proactive traffic management,and aids in creating smarter,more sustainable cities.

The real-time dynamic holographic display of LN:Bi,Mg crystals and defect-related electron mobility

Holographic display has attracted widespread interest because of its ability to show the complete information of the object and bring people an unprecedented sense of presence. The absence of ideal recording materials has hampered the realization of their commercial applications. Here we report that the response time of a bismuth and magnesium codoped lithium niobate(LN:Bi,Mg) crystal is shortened to 7.2 ms and a sensitivity as high as 646 cm/J. The crystal was used to demonstrate a real-time holographic display with a refresh rate of 60 Hz, as that of the popular high-definition television. Moreover, the first-principles calculations indicate that the electron mobility while Bi occupying Nb-site is significantly greater than that in Li-site, which directly induces the fast response of LN:Bi,Mg crystals when the concentration of Mg is above its doping threshold.

Real-Time Vivo Tooth Mobility Measurement and Analysis

The significance and research status of tooth mobility (TM) measurement are introduced. There are many methods to measure the TM, but up to now no one does vivo dynamic TM measurement. An optical-electronic method is raised to measure it in this paper. The measurement theory is introduced and a measurement system is setup. Special proceeding is done for this measurement because of the small oral cavity measurement area. It is the first time to get vivo dynamic TM measurement result at last. The measurement result is analysed and a new movement period is discovered. It shows that this method satisfies for the dynamical tooth movement. Further research is suggested.

基于Real-time PCR法检测乳粉中牛源性成分定量研究

基于Real-timePCR建立了乳粉中牛源性成分相对定量检测方法,并对牛的特异性引物与探针进行了特异性、灵敏度和稳定性测试。通过模拟不同浓度牛乳粉与马乳粉混合样本,根据其△Ct值的函数关系进行线性拟合进而绘制标准曲线,建立乳粉中牛源性成分的相对定量检测。结果显示,该方法的最低检测限为0.00001 mg/mL,回收率为91.11%~119.2%,组间变异系数≤0.58%、组内变异系数≤1.44%。说明该方法在特异性与稳定性上适用于乳粉中牛源性成分及含量的掺假检测。

A multifunctional shear apparatus for rocks subjected to true triaxial stress and high temperature in real-time

Deep engineering disasters,such as rockbursts and collapses,are more related to the shear slip of rock joints.A novel multifunctional device was developed to study the shear failure mechanism in rocks.Using this device,the complete shearedeformation process and long-term shear creep tests could be performed on rocks under constant normal stiffness(CNS)or constant normal loading(CNL)conditions in real-time at high temperature and true-triaxial stress.During the research and development process,five key technologies were successfully broken through:(1)the ability to perform true-triaxial compressioneshear loading tests on rock samples with high stiffness;(2)a shear box with ultra-low friction throughout the entire stress space of the rock sample during loading;(3)a control system capable of maintaining high stress for a long time and responding rapidly to the brittle fracture of a rock sample as well;(4)a refined ability to measure the volumetric deformation of rock samples subjected to true triaxial shearing;and(5)a heating system capable of maintaining uniform heating of the rock sample over a long time.By developing these technologies,loading under high true triaxial stress conditions was realized.The apparatus has a maximum normal stiffness of 1000 GPa/m and a maximum operating temperature of 300C.The differences in the surface temperature of the sample are constant to within5C.Five types of true triaxial shear tests were conducted on homogeneous sandstone to verify that the apparatus has good performance and reliability.The results show that temperature,lateral stress,normal stress and time influence the shear deformation,failure mode and strength of the sandstone.The novel apparatus can be reliably used to conduct true-triaxial shear tests on rocks subjected to high temperatures and stress.

Pore-pressure and stress-coupled creep behavior in deep coal:Insights from real-time NMR analysis

Understanding the variations in microscopic pore-fracture structures(MPFS) during coal creep under pore pressure and stress coupling is crucial for coal mining and effective gas treatment. In this manuscript, a triaxial creep test on deep coal at various pore pressures using a test system that combines in-situ mechanical loading with real-time nuclear magnetic resonance(NMR) detection was conducted.Full-scale quantitative characterization, online real-time detection, and visualization of MPFS during coal creep influenced by pore pressure and stress coupling were performed using NMR and NMR imaging(NMRI) techniques. The results revealed that seepage pores and microfractures(SPM) undergo the most significant changes during coal creep, with creep failure gradually expanding from dense primary pore fractures. Pore pressure presence promotes MPFS development primarily by inhibiting SPM compression and encouraging adsorption pores(AP) to evolve into SPM. Coal enters the accelerated creep stage earlier at lower stress levels, resulting in more pronounced creep deformation. The connection between the micro and macro values was established, demonstrating that increased porosity at different pore pressures leads to a negative exponential decay of the viscosity coefficient. The Newton dashpot in the ideal viscoplastic body and the Burgers model was improved using NMR experimental results, and a creep model that considers pore pressure and stress coupling using variable-order fractional operators was developed. The model’s reasonableness was confirmed using creep experimental data. The damagestate adjustment factors ω and β were identified through a parameter sensitivity analysis to characterize the effect of pore pressure and stress coupling on the creep damage characteristics(size and degree of difficulty) of coal.

一种基于real-time PCR技术的TTV检测方法的建立及应用

目的:本研究旨在开发一种具有更高灵敏度和特异性的TTV检测技术,为揭示TTV在多种疾病过程中的作用提供重要的技术支持。方法:为了更精确、灵敏的检测TTV,本研究分析了目前公布的所有亚型的TTV基因序列,在此基础上建立了一种基于UTR区域的real-time PCR检测方法,并与文献报道应用较为广泛的PCR检测方法进行了对比。结果:本研究建立的方法在1×10^(7)~1×10^(1) copies/μL标准品浓度范围内具有良好的线性关系,相关系数为1.000,斜率为-3.446,检测下限为1×10^(1) copies/μL。重复性试验结果显示,组内变异系数为7.22%,表明本方法重复性、稳定性较强。针对30份临床样本,使用本研究建立的real-time PCR检测方法及目前被多个研究所使用的4套引物进行对比。结果表明,本研究所建立的方法灵敏度显著高于文献中报道的4种方法(P<0.01);Sanger测序结果表明,本方法检测出的30份阳性样本均为TTV,检测特异性为100%。结论:本研究采用基于TaqMan探针的real-time PCR检测方法,检测灵敏性高、覆盖基因型范围广,尤其对于TTV病毒载量较低的情况下能够进行定量检测,对于TTV病毒的致病性及作为免疫标志物的应用提供重要的技术支持。

Scheduling transactions in mobile distributed real-time database systems

A DMVOCC-MVDA （distributed multiversion optimistic concurrency control with multiversion dynamic adjustment） protocol was presented to process mobile distributed real-time transaction in mobile broadcast environments. At the mobile hosts, all transactions perform local pre-validation. The local pre-validation process is carried out against the committed transactions at the server in the last broadcast cycle. Transactions that survive in local pre-validation must be submitted to the server for local final validation. The new protocol eliminates conflicts between mobile read-only and mobile update transactions, and resolves data conflicts flexibly by using multiversion dynamic adjustment of serialization order to avoid unnecessary restarts of transactions. Mobile read-only transactions can be committed with no-blocking, and respond time of mobile read-only transactions is greatly shortened. The tolerance of mobile transactions of disconnections from the broadcast channel is increased. In global validation mobile distributed transactions have to do check to ensure distributed serializability in all participants. The simulation results show that the new concurrency control protocol proposed offers better performance than other protocols in terms of miss rate, restart rate, commit rate. Under high work load （think time is ls） the miss rate of DMVOCC-MVDA is only 14.6%, is significantly lower than that of other protocols. The restart rate of DMVOCC-MVDA is only 32.3%, showing that DMVOCC-MVDA can effectively reduce the restart rate of mobile transactions. And the commit rate of DMVOCC-MVDA is up to 61.2%, which is obviously higher than that of other protocols.

Novel Cs-Mg-Al mixed oxide with improved mobility of oxygen species for passive NO_(x)adsorption

The development of passive NO_(x)adsorbers with cost-benefit and high NO_(x)storage capacity remains an on-going challenge to after-treatment technologies at lower temperatures associated with cold-start NO_(x)emissions.Herein,Cs_(1)Mg_(3)Al catalyst prepared by sol-gel method was cyclic tested in NO_(x)storage under 5 vol%water.At 100°C,the NO_(x)storage capacity(1219 μmol g^(-1))was much higher than that of Pt/BaO/Al_(2)O_(3)(610 μmol g^(-1)).This provided new insights for non-noble metal catalysts in low-temperature passive NO_(x)adsorption.The addition of Cs improved the mobility of oxygen species and thus improved the NO_(x)storage capacity.The XRD,XPS,IR spectra and in situ DRIFTs with NH3 probe showed an interaction between CsO_(x)and AlO_(x)sites via oxygen species formed on Cs_(1)Mg_(3)Al catalyst.The improved mobility of oxygen species inferred from O2-TPD was consistent with high NO_(x)storage capacity related to enhanced formation of nitrate and additional nitrite species by NO_(x)oxidation.Moreover,the addition of Mg might improve the stability of Cs_(1)Mg_(3)Al by stabilizing surface active oxygen species in cyclic experiments.

On-line real-time path planning of mobile robots in dynamic uncertain environment

A new path planning method for mobile robots in globally unknown environment with moving obstacles is pre- sented. With an autoregressive (AR) model to predict the future positions of moving obstacles, and the predicted position taken as the next position of moving obstacles, a motion path in dynamic uncertain environment is planned by means of an on-line real-time path planning technique based on polar coordinates in which the desirable direction angle is taken into consideration as an optimization index. The effectiveness, feasibility, high stability, perfect performance of obstacle avoidance, real-time and optimization capability are demonstrated by simulation examples.

Web-based GIS System for Real-time Field Data Collection Using Personal Mobile Phone

Recently, use of mobile communicational devices in field data collection is increasing such as smart phones and cellular phones due to emergence of embedded Global Position System GPS and Wi-Fi Internet access. Accurate timely and handy field data collection is required for disaster management and emergency quick responses. In this article, we introduce web-based GIS system to collect the field data by personal mobile phone through Post Office Protocol POP3 mail server. The main objective of this work is to demonstrate real-time field data collection method to the students using their mobile phone to collect field data by timely and handy manners, either individual or group survey in local or global scale research.

A Concept of Dynamically Reconfigurable Real-time Vision System for Autonomous Mobile Robotics

This paper describes specific constraints of vision systems that are dedicated to be embedded in mobile robots. If PC-based hardware architecture is convenient in this field because of its versatility, flexibility, performance, and cost, current real-time operating systems are not completely adapted to long processing with varying duration, and it is often necessary to oversize the system to guarantee fail-safe functioning. Also, interactions with other robotic tasks having more priority are difficult to handle. To answer this problem, we have developed a dynamically reconfigurable vision processing system, based on the innovative features of Cleopatre real-time applicative layer concerning scheduling and fault tolerance. This framework allows to define emergency and optional tasks to ensure a minimal quality of service for the other subsystems of the robot, while allowing to adapt dynamically vision processing chain to an exceptional overlasting vision process or processor overload. Thus, it allows a better cohabitation of several subsystems in a single hardware, and to develop less expensive but safe systems, as they will be designed for the regular case and not rare exceptional ones. Finally, it brings a new way to think and develop vision systems, with pairs of complementary operators.

Bone marrow-derived mesenchymal stem cell-derived exosomeloaded miR-129-5p targets high-mobility group box 1 attenuates neurological-impairment after diabetic cerebral hemorrhage

BACKGROUND Diabetic intracerebral hemorrhage(ICH)is a serious complication of diabetes.The role and mechanism of bone marrow mesenchymal stem cell(BMSC)-derived exosomes(BMSC-exo)in neuroinflammation post-ICH in patients with diabetes are unknown.In this study,we investigated the regulation of BMSC-exo on hyperglycemia-induced neuroinflammation.AIM To study the mechanism of BMSC-exo on nerve function damage after diabetes complicated with cerebral hemorrhage.METHODS BMSC-exo were isolated from mouse BMSC media.This was followed by transfection with microRNA-129-5p(miR-129-5p).BMSC-exo or miR-129-5poverexpressing BMSC-exo were intravitreally injected into a diabetes mouse model with ICH for in vivo analyses and were cocultured with high glucoseaffected BV2 cells for in vitro analyses.The dual luciferase test and RNA immunoprecipitation test verified the targeted binding relationship between miR-129-5p and high-mobility group box 1(HMGB1).Quantitative polymerase chain reaction,western blotting,and enzyme-linked immunosorbent assay were conducted to assess the levels of some inflammation factors,such as HMGB1,interleukin 6,interleukin 1β,toll-like receptor 4,and tumor necrosis factorα.Brain water content,neural function deficit score,and Evans blue were used to measure the neural function of mice.RESULTS Our findings indicated that BMSC-exo can promote neuroinflammation and functional recovery.MicroRNA chip analysis of BMSC-exo identified miR-129-5p as the specific microRNA with a protective role in neuroinflammation.Overexpression of miR-129-5p in BMSC-exo reduced the inflammatory response and neurological impairment in comorbid diabetes and ICH cases.Furthermore,we found that miR-129-5p had a targeted binding relationship with HMGB1 mRNA.CONCLUSION We demonstrated that BMSC-exo can reduce the inflammatory response after ICH with diabetes,thereby improving the neurological function of the brain.

Real-Time Urban Traffic State Estimation with A-GPS Mobile Phones as Probes

This paper presents a microscopic traffic simulation-based method for urban traffic state estimation using Assisted Global Positioning System (A-GPS) mobile phones. In this approach, real-time location data are collected by A-GPS mobile phones to track vehicles traveling on urban roads. In addition, tracking data obtained from individual mobile probes are aggregated to provide estimations of average road link speeds along rolling time periods. Moreover, the estimated average speeds are classified to different traffic condition levels, which are prepared for displaying a real-time traffic map on mobile phones. Simulation results demonstrate the effectiveness of the proposed method, which are fundamental for the subsequent development of a system demonstrator.

A Fault-Tolerant Mobility-Aware Caching Method in Edge Computing

Mobile Edge Computing(MEC)is a technology designed for the on-demand provisioning of computing and storage services,strategically positioned close to users.In the MEC environment,frequently accessed content can be deployed and cached on edge servers to optimize the efficiency of content delivery,ultimately enhancing the quality of the user experience.However,due to the typical placement of edge devices and nodes at the network’s periphery,these components may face various potential fault tolerance challenges,including network instability,device failures,and resource constraints.Considering the dynamic nature ofMEC,making high-quality content caching decisions for real-time mobile applications,especially those sensitive to latency,by effectively utilizing mobility information,continues to be a significant challenge.In response to this challenge,this paper introduces FT-MAACC,a mobility-aware caching solution grounded in multi-agent deep reinforcement learning and equipped with fault tolerance mechanisms.This approach comprehensively integrates content adaptivity algorithms to evaluate the priority of highly user-adaptive cached content.Furthermore,it relies on collaborative caching strategies based onmulti-agent deep reinforcement learningmodels and establishes a fault-tolerancemodel to ensure the system’s reliability,availability,and persistence.Empirical results unequivocally demonstrate that FTMAACC outperforms its peer methods in cache hit rates and transmission latency.

Real-time data processing method for CO_(2) dispersion interferometer on EAST

A real-time data processing system is designed for the carbon dioxide dispersion interferometer(CO_(2)-DI)on EAST.The system utilizes the parallel and pipelining capabilities of an fieldprogrammable gate array(FPGA)to digitize and process the intensity of signals from the detector.Finally,the real-time electron density signals are exported through a digital-to-analog converter(DAC)module in the form of analog signals.The system has been successfully applied in the CO_(2)-DI system to provide low-latency electron density input to the plasma control system on EAST.Experimental results of the latest campaign with long-pulse discharges on EAST(2022–2023)demonstrate that the system can respond effectively in the case of rapid density changes,proving its reliability and accuracy for future electron density calculation.

Real-Time Intelligent Diagnosis of Co-frequency Vibration Faults in Rotating Machinery Based on Lightweight-Convolutional Neural Networks

The co-frequency vibration fault is one of the common faults in the operation of rotating equipment,and realizing the real-time diagnosis of the co-frequency vibration fault is of great significance for monitoring the health state and carrying out vibration suppression of the equipment.In engineering scenarios,co-frequency vibration faults are highlighted by rotational frequency and are difficult to identify,and existing intelligent methods require more hardware conditions and are exclusively time-consuming.Therefore,Lightweight-convolutional neural networks(LW-CNN)algorithm is proposed in this paper to achieve real-time fault diagnosis.The critical parameters are discussed and verified by simulated and experimental signals for the sliding window data augmentation method.Based on LW-CNN and data augmentation,the real-time intelligent diagnosis of co-frequency is realized.Moreover,a real-time detection method of fault diagnosis algorithm is proposed for data acquisition to fault diagnosis.It is verified by experiments that the LW-CNN and sliding window methods are used with high accuracy and real-time performance.

Mobility edges and localization characteristics in one-dimensional quasiperiodic quantum walk

We construct a one-dimensional quasiperiodic quantum walk to investigate the localization–delocalization transition.The inverse participation ratio and Lyapunov exponent are employed as two indexes to determine the mobility edge, a critical energy to distinguish the energy regions of extended and localized states. The analytical solution of mobility edge is obtained by the Lyapunov exponents in global theory, and the consistency of the two indexes is confirmed. We further study the dynamic characteristics of the quantum walk and show that the probabilities are localized to some specific lattice sites with time evolution. This phenomenon is explained by the effective potential of the Hamiltonian which corresponds to the phase in the coin operator of the quantum walk.