The effect of triptolide （TP） on the expression of receptor activator of nuclear factor-κB ligand （RANKL） and osteoprotegerin （OPG） was explored in rat adjuvant induced arthritis （AA）. AA was induced in Wista...The effect of triptolide （TP） on the expression of receptor activator of nuclear factor-κB ligand （RANKL） and osteoprotegerin （OPG） was explored in rat adjuvant induced arthritis （AA）. AA was induced in Wistar rats. Arthritis rats were treated with TP and methotrexate （MTX） at the onset （day 9） of arthritis. On the peak of arthritis （day 24）, the expression of RANKL and OPG protein in the joints and RANKL mRNA in peripheral blood mononuclear cells （PBMC） was detected. TNF-α and IL-1β levels in peripheral blood were determined. Bone erosion scores were also evaluated. The results showed that bone erosion scores in TP and MTX groups were lower than in AA group （.P〈0.01） ; The expression levels of RANKL in the synovium （P〈0.01） and bone （P〈0.05）, and OPG level in synovium （P〈0.05） were lower in TP group than in AA group （P〈0.05）. In TP group, the expression levels of RANKL mRNA and TNF-α, IL-1β in PBMC were lower than in AA group （all P〈0.01）. It was concluded that TP could inhibit rat adjuvant arthritis bone erosion by suppressing the expression of RANKL.展开更多
Objective To study the effect of baicalin on the expression of receptor activator of nuclear factor-κB ligand(RANKL)and osteoprotegerin(OPG)in cultured human periodontal ligament(HPDL)cells.Methods Small interfering ...Objective To study the effect of baicalin on the expression of receptor activator of nuclear factor-κB ligand(RANKL)and osteoprotegerin(OPG)in cultured human periodontal ligament(HPDL)cells.Methods Small interfering RNA(siRNA)eukaryotic expression vector targeted transforming growth factor βⅡ receptor(TGF-β RⅡ)was constructed and transfected into T cells.HPDL cells with T cells transfected with siRNA or not were placed in the culture medium that had been added with lipopolysaccharide(LPS)and baicalin.The obtained solution was divided into six groups according to the components(group Ⅰ:HPDL cells+LPS+T cells transfected with siRNA1+baicalin;group Ⅱ:HPDL cells+LPS+T cells transfected with siRNA1;group Ⅲ:HPDL cells+LPS+T cells+baicalin;group Ⅳ:HPDL cells+LPS+T cells;group Ⅴ:HPDL cells+baicalin;group Ⅵ:HPDL cells)and was cultured for 48 hours.RT-PCR was used to observe the effect of baicalin on the expression of OPG-RANKL in HPDL cells.Results The ratio of RANKL/OPG in group Ⅰ was lower than that in group Ⅱ(P<0.01)and higher than that in group Ⅲ(P<0.01);The ratio of RANKL/OPG in group Ⅲ was lower than that in group Ⅳ(P<0.01);the ratio of RANKL/OPG in group Ⅳ was higher than that in group Ⅵ(P<0.01);the ratio of RANKL/OPG in group Ⅴ was lower than that in group Ⅵ(P<0.05).Conclusion ① Baicalin could decrease the ratio of RANKL/OPG in HPDL cells.② The TGF-β signaling transduction plays an important role in the effect of baicalin on the RANKL/OPG ratio in HPDL cells.③ Baicalin acts not only through TGF-β to regulate RANKL/OPG in HPDL cells,but also through other pathways.展开更多
Background:Obstructive sleep apnea-hypopnea syndrome (OSAHS) is associated with a higher prevalence of osteoporosis.However,the underlying mechanisms linking OSAHS with bone loss are still unclear.The aim of this stud...Background:Obstructive sleep apnea-hypopnea syndrome (OSAHS) is associated with a higher prevalence of osteoporosis.However,the underlying mechanisms linking OSAHS with bone loss are still unclear.The aim of this study was to investigate the changes of receptor activator of nuclear factor-κB ligand (RANKL,an osteoclastogenesis-promoting factor) and osteoprotegerin (OPG,the decoy receptor for RANKL),oxidative stress and bone metabolism markers in OSAHS,in order to understand the potential mechanisms underlying bone loss in OSAHS patients.Methods:Forty-eight male patients with OSAHS,confirmed by polysomnography (PSG) study,were enrolled.Twenty male subjects who were confirmed as not having OSAHS served as the controls.The subjects’bone mineral density (BMD) was assessed in lumbar spine and femoral neck using dual-energy X-ray absorptiometry (DXA).Blood samples were collected from all subjects for measurement of RANKL,OPG,the bone formation marker bone-specific alkaline phosphatase (BAP),the bone resorption marker tartrate-resistant acid phosphatase 5b (TRAP-5b),and total antioxidant capacity (TAOC).Results:The BMD and the T-score of the femoral neck and the lumbar spine were significantly lower in OSAHS patients as compared to the control group (P< 0.05).The serum level of BAP was significantly decreased in the OSAHS group (15.62 ± 5.20 μg/L) as compared to the control group (18.83 ± 5.50 μg/L,t= -2.235,P< 0.05),while the levels of TRAP-5b did not differ between the two groups (t= -1.447,P> 0.05).The serum level of OPG and the OPG/RANKL ratio were lower in the OSAHS group compared to the control group (bothP< 0.05).TAOC level was also decreased significantly in the OSAHS group (P< 0.05).Correlation analysis showed that the TAOC level was positively correlated with BAP in the OSAHS group (r= 0.248,P= 0.04),but there were no correlations between TAOC and the BMD or the T-scores.The correlations between the level of OPG (or the OPG/RANKL ratio) and BMD or TAOC did not reach significance.Conclusion:In OSAHS patients,lower levels of TAOC were associated with decreased bone formation,suggesting a role of oxidative stress in bone loss,while the role of OPG/RANKL imbalance in bone metabolism in OSAHS needs further evaluation .展开更多
目的:对正常健康人群外周血液中骨保护素(Osteoprotegerin,OPG)、核因子kappaB受体活化因子配体(receptor activator of NFκBligand,RANKL)浓度测定,明确其与年龄、性别之间是否存在一定关系。方法:收集220例正常健康人的清晨外周血液...目的:对正常健康人群外周血液中骨保护素(Osteoprotegerin,OPG)、核因子kappaB受体活化因子配体(receptor activator of NFκBligand,RANKL)浓度测定,明确其与年龄、性别之间是否存在一定关系。方法:收集220例正常健康人的清晨外周血液,其中男108例,女112例;年龄35～70岁,平均52.5岁。用ELISA法测血清中OPG、sRANKL浓度。结果:在35～70岁正常健康人群外周血液中的OPG、sRANKL浓度:女性,OPG21.95～315.47pg/ml,sRANKL10.25～370.20pmol/L;男性,OPG14.78～192.55pg/ml,sRANKL9.25～300.32pmol/L。46岁后女性血液中OPG浓度与年龄存在正相关;57岁后女性血液中sRANKL浓度明显升高。结论:年龄和性别影响外周血液中OPG、sRANKL浓度。46岁后女性血液中OPG浓度随年龄增加而升高,女性血液中sRANKL浓度57岁后明显升高。展开更多
目的研究初发系统性红斑狼疮（systemic lupus erythematosus，SLE）患者外周血淋巴细胞中细胞核因子-κB受体激活剂配体（receptor activator of nuclear factor kappa B ligand，RANKL）、护骨素（osteoprotegerin，OPG）基因mRNA的...目的研究初发系统性红斑狼疮（systemic lupus erythematosus，SLE）患者外周血淋巴细胞中细胞核因子-κB受体激活剂配体（receptor activator of nuclear factor kappa B ligand，RANKL）、护骨素（osteoprotegerin，OPG）基因mRNA的表达情况，探讨其表达水平与初发SLE患者骨质疏松的关系。方法选择初发SLE患者45例及正常对照42例，运用实时定量PCR方法检测患者外周血淋巴细胞RANKL、OPG的mRNA表达水平。采用双能X线骨密度仪分别检测患者腰椎（L1-4）和股骨近端2个部位的骨密度，单因素分析RANKL、OPG基因mRNA表达水平与SLE患者骨密度的关系。结果SLE患者RANKL、OPG基因mRNA表达水平较正常对照组明显减低（P〈0．01）；SLE患者2个部位的骨密度均低于正常对照组（P〈0．05），骨量异常发生率为28-89％，骨量异常降低的SLE患者OPG基因mRNA的表达水平比骨量正常的患者显著降低，两者间的差异有统计学意义（P〈0．01）；而RANKL基因mRNA表达水平的差异无统计学意义（P〉0．05）；OPG基因mRNA表达水平与初发SLE患者骨密度间存在正相关（r=0．461；P=0．001），即OPG表达水平越低，骨量减少越明显；而RANKL基因mRNA表达降低与初发SLE患者骨密度无明显相关性（r=-0．189，P=0．214）；初发SLE患者疾病活动度与骨量减少、RANKL及OPG基因表达水平间不存在相关性（r=0．293，P=0．138；r=-0．099，P=0．493；，=0．138，P=0．493）。结论初发SLE患者骨量减少的发病率较正常人群增高，并且初发SLE患者体内RANKL和OPG基因表达存在异常；其中OPG表达水平的降低可能与初发SLE患者的骨量减少有密切关系。展开更多
目的分析血清骨保护素(osteoprotegerin,OPG)、核因子κB活化因子配体(receptor-activator of nuclear factor kappa B ligand,RANKL)及雌二醇(estradiol,E_2)对运动性骨疲劳的早期筛查价值。方法健康雌性大鼠随机分为对照组和实验组,8...目的分析血清骨保护素(osteoprotegerin,OPG)、核因子κB活化因子配体(receptor-activator of nuclear factor kappa B ligand,RANKL)及雌二醇(estradiol,E_2)对运动性骨疲劳的早期筛查价值。方法健康雌性大鼠随机分为对照组和实验组,8周训练结束后处死大鼠,采用酶联免疫法检测血清RANKL、OPG和E_2水平,采用IBM 21.0 SPSS绘制受试者工作特征曲线曲线(receiver operating characteristic curve,ROC曲线),计算它们的ROC曲线下面积(area under the curve,AUC)、灵敏度和特异度,根据约登指数确定其诊断界值并评价筛查价值。结果血清RANKL、OPG、OPG/RANKL比值及E_2的AUC均大于0.9。选定的血清RANKL、OPG、OPG/RANKL比值和E_2的诊断界值分别为96.245 ng/ml、2.383 ng/ml、0.028和105.293 pg/ml,其灵敏度和特异度分别为90.21%和45.22%、96.19%和51.07%、94.42%和56.69%、93.36%和40.71%。血清RANKL、OPG、OPG/RANKL比值和E_2均早于金标准出现阳性诊断结果。结论动态检测血清RANKL、OPG及E_2水平,有望实现运动性骨疲劳的早期预警。展开更多
文摘The effect of triptolide （TP） on the expression of receptor activator of nuclear factor-κB ligand （RANKL） and osteoprotegerin （OPG） was explored in rat adjuvant induced arthritis （AA）. AA was induced in Wistar rats. Arthritis rats were treated with TP and methotrexate （MTX） at the onset （day 9） of arthritis. On the peak of arthritis （day 24）, the expression of RANKL and OPG protein in the joints and RANKL mRNA in peripheral blood mononuclear cells （PBMC） was detected. TNF-α and IL-1β levels in peripheral blood were determined. Bone erosion scores were also evaluated. The results showed that bone erosion scores in TP and MTX groups were lower than in AA group （.P〈0.01） ; The expression levels of RANKL in the synovium （P〈0.01） and bone （P〈0.05）, and OPG level in synovium （P〈0.05） were lower in TP group than in AA group （P〈0.05）. In TP group, the expression levels of RANKL mRNA and TNF-α, IL-1β in PBMC were lower than in AA group （all P〈0.01）. It was concluded that TP could inhibit rat adjuvant arthritis bone erosion by suppressing the expression of RANKL.
基金supported by the Foundation of Stomatology Hospital,Xi'an Jiaotong University
文摘Objective To study the effect of baicalin on the expression of receptor activator of nuclear factor-κB ligand(RANKL)and osteoprotegerin(OPG)in cultured human periodontal ligament(HPDL)cells.Methods Small interfering RNA(siRNA)eukaryotic expression vector targeted transforming growth factor βⅡ receptor(TGF-β RⅡ)was constructed and transfected into T cells.HPDL cells with T cells transfected with siRNA or not were placed in the culture medium that had been added with lipopolysaccharide(LPS)and baicalin.The obtained solution was divided into six groups according to the components(group Ⅰ:HPDL cells+LPS+T cells transfected with siRNA1+baicalin;group Ⅱ:HPDL cells+LPS+T cells transfected with siRNA1;group Ⅲ:HPDL cells+LPS+T cells+baicalin;group Ⅳ:HPDL cells+LPS+T cells;group Ⅴ:HPDL cells+baicalin;group Ⅵ:HPDL cells)and was cultured for 48 hours.RT-PCR was used to observe the effect of baicalin on the expression of OPG-RANKL in HPDL cells.Results The ratio of RANKL/OPG in group Ⅰ was lower than that in group Ⅱ(P<0.01)and higher than that in group Ⅲ(P<0.01);The ratio of RANKL/OPG in group Ⅲ was lower than that in group Ⅳ(P<0.01);the ratio of RANKL/OPG in group Ⅳ was higher than that in group Ⅵ(P<0.01);the ratio of RANKL/OPG in group Ⅴ was lower than that in group Ⅵ(P<0.05).Conclusion ① Baicalin could decrease the ratio of RANKL/OPG in HPDL cells.② The TGF-β signaling transduction plays an important role in the effect of baicalin on the RANKL/OPG ratio in HPDL cells.③ Baicalin acts not only through TGF-β to regulate RANKL/OPG in HPDL cells,but also through other pathways.
文摘Background:Obstructive sleep apnea-hypopnea syndrome (OSAHS) is associated with a higher prevalence of osteoporosis.However,the underlying mechanisms linking OSAHS with bone loss are still unclear.The aim of this study was to investigate the changes of receptor activator of nuclear factor-κB ligand (RANKL,an osteoclastogenesis-promoting factor) and osteoprotegerin (OPG,the decoy receptor for RANKL),oxidative stress and bone metabolism markers in OSAHS,in order to understand the potential mechanisms underlying bone loss in OSAHS patients.Methods:Forty-eight male patients with OSAHS,confirmed by polysomnography (PSG) study,were enrolled.Twenty male subjects who were confirmed as not having OSAHS served as the controls.The subjects’bone mineral density (BMD) was assessed in lumbar spine and femoral neck using dual-energy X-ray absorptiometry (DXA).Blood samples were collected from all subjects for measurement of RANKL,OPG,the bone formation marker bone-specific alkaline phosphatase (BAP),the bone resorption marker tartrate-resistant acid phosphatase 5b (TRAP-5b),and total antioxidant capacity (TAOC).Results:The BMD and the T-score of the femoral neck and the lumbar spine were significantly lower in OSAHS patients as compared to the control group (P< 0.05).The serum level of BAP was significantly decreased in the OSAHS group (15.62 ± 5.20 μg/L) as compared to the control group (18.83 ± 5.50 μg/L,t= -2.235,P< 0.05),while the levels of TRAP-5b did not differ between the two groups (t= -1.447,P> 0.05).The serum level of OPG and the OPG/RANKL ratio were lower in the OSAHS group compared to the control group (bothP< 0.05).TAOC level was also decreased significantly in the OSAHS group (P< 0.05).Correlation analysis showed that the TAOC level was positively correlated with BAP in the OSAHS group (r= 0.248,P= 0.04),but there were no correlations between TAOC and the BMD or the T-scores.The correlations between the level of OPG (or the OPG/RANKL ratio) and BMD or TAOC did not reach significance.Conclusion:In OSAHS patients,lower levels of TAOC were associated with decreased bone formation,suggesting a role of oxidative stress in bone loss,while the role of OPG/RANKL imbalance in bone metabolism in OSAHS needs further evaluation .
文摘目的:对正常健康人群外周血液中骨保护素(Osteoprotegerin,OPG)、核因子kappaB受体活化因子配体(receptor activator of NFκBligand,RANKL)浓度测定,明确其与年龄、性别之间是否存在一定关系。方法:收集220例正常健康人的清晨外周血液,其中男108例,女112例;年龄35～70岁,平均52.5岁。用ELISA法测血清中OPG、sRANKL浓度。结果:在35～70岁正常健康人群外周血液中的OPG、sRANKL浓度:女性,OPG21.95～315.47pg/ml,sRANKL10.25～370.20pmol/L;男性,OPG14.78～192.55pg/ml,sRANKL9.25～300.32pmol/L。46岁后女性血液中OPG浓度与年龄存在正相关;57岁后女性血液中sRANKL浓度明显升高。结论:年龄和性别影响外周血液中OPG、sRANKL浓度。46岁后女性血液中OPG浓度随年龄增加而升高,女性血液中sRANKL浓度57岁后明显升高。
文摘目的研究初发系统性红斑狼疮（systemic lupus erythematosus，SLE）患者外周血淋巴细胞中细胞核因子-κB受体激活剂配体（receptor activator of nuclear factor kappa B ligand，RANKL）、护骨素（osteoprotegerin，OPG）基因mRNA的表达情况，探讨其表达水平与初发SLE患者骨质疏松的关系。方法选择初发SLE患者45例及正常对照42例，运用实时定量PCR方法检测患者外周血淋巴细胞RANKL、OPG的mRNA表达水平。采用双能X线骨密度仪分别检测患者腰椎（L1-4）和股骨近端2个部位的骨密度，单因素分析RANKL、OPG基因mRNA表达水平与SLE患者骨密度的关系。结果SLE患者RANKL、OPG基因mRNA表达水平较正常对照组明显减低（P〈0．01）；SLE患者2个部位的骨密度均低于正常对照组（P〈0．05），骨量异常发生率为28-89％，骨量异常降低的SLE患者OPG基因mRNA的表达水平比骨量正常的患者显著降低，两者间的差异有统计学意义（P〈0．01）；而RANKL基因mRNA表达水平的差异无统计学意义（P〉0．05）；OPG基因mRNA表达水平与初发SLE患者骨密度间存在正相关（r=0．461；P=0．001），即OPG表达水平越低，骨量减少越明显；而RANKL基因mRNA表达降低与初发SLE患者骨密度无明显相关性（r=-0．189，P=0．214）；初发SLE患者疾病活动度与骨量减少、RANKL及OPG基因表达水平间不存在相关性（r=0．293，P=0．138；r=-0．099，P=0．493；，=0．138，P=0．493）。结论初发SLE患者骨量减少的发病率较正常人群增高，并且初发SLE患者体内RANKL和OPG基因表达存在异常；其中OPG表达水平的降低可能与初发SLE患者的骨量减少有密切关系。
文摘目的分析血清骨保护素(osteoprotegerin,OPG)、核因子κB活化因子配体(receptor-activator of nuclear factor kappa B ligand,RANKL)及雌二醇(estradiol,E_2)对运动性骨疲劳的早期筛查价值。方法健康雌性大鼠随机分为对照组和实验组,8周训练结束后处死大鼠,采用酶联免疫法检测血清RANKL、OPG和E_2水平,采用IBM 21.0 SPSS绘制受试者工作特征曲线曲线(receiver operating characteristic curve,ROC曲线),计算它们的ROC曲线下面积(area under the curve,AUC)、灵敏度和特异度,根据约登指数确定其诊断界值并评价筛查价值。结果血清RANKL、OPG、OPG/RANKL比值及E_2的AUC均大于0.9。选定的血清RANKL、OPG、OPG/RANKL比值和E_2的诊断界值分别为96.245 ng/ml、2.383 ng/ml、0.028和105.293 pg/ml,其灵敏度和特异度分别为90.21%和45.22%、96.19%和51.07%、94.42%和56.69%、93.36%和40.71%。血清RANKL、OPG、OPG/RANKL比值和E_2均早于金标准出现阳性诊断结果。结论动态检测血清RANKL、OPG及E_2水平,有望实现运动性骨疲劳的早期预警。
