[ Objective] To find an efficient and low-consumption estrus synchronization method and thus to provide the basis for embryo transfer. [Method] A total of 120 healthy multiparous yellow cattle were selected as recepto...[ Objective] To find an efficient and low-consumption estrus synchronization method and thus to provide the basis for embryo transfer. [Method] A total of 120 healthy multiparous yellow cattle were selected as receptor cows for embryo transfer and assigned into three groups ran- domly ( n = 40). To induce estrous synchronization, the cattle in the group I were injected 4 mg prostaglandin (PG) ; those in the group II were first transplanted with vaginal embolism and then injected with 4 mg PG; and those in the group III were first transplanted with vaginal embolism and then injected with 0.4 mg PG and 2.0 ml follicle-stimulating hormone (FSH). The rate of estrous synchronization, rate of embryo transfer and rejec- tion rate were compared between different treatments. [Result] The rates of estrous synchronization of the group I, II and III were 0%, 80% and 78%, respectively, and their rates of embryo transfer were 27.5%, 60.0% and 45.0%, respectively. [ Conclusion] Transplantation with vaginal embolism followed by injection with PG and FSH is the optimum method to induce estrus synchronization in yellow cattle.展开更多
With the enlargement of intensive feeding scale of dairy cows and improvement of milk yield, the incidence rate of displacement of abomasum in dairy cows increases gradually in recent years. Previous researches have s...With the enlargement of intensive feeding scale of dairy cows and improvement of milk yield, the incidence rate of displacement of abomasum in dairy cows increases gradually in recent years. Previous researches have shown that VFA in abomasums can significantly inhibit the shrinkage of smooth muscles of abomasums and lead to atony of abomasums, but the specific regulatory mechanisms are still unclear. In this study, the expression of VFA receptors GPR41 and GPR43 in cow abomasums were detected by PCR method, and the fragments were consistent with the excepted sizes. This study provided theoretical basis for the pathologic mechanism investigation, prevention and treatment of displacement of abomasum in dairy cows.展开更多
文摘[ Objective] To find an efficient and low-consumption estrus synchronization method and thus to provide the basis for embryo transfer. [Method] A total of 120 healthy multiparous yellow cattle were selected as receptor cows for embryo transfer and assigned into three groups ran- domly ( n = 40). To induce estrous synchronization, the cattle in the group I were injected 4 mg prostaglandin (PG) ; those in the group II were first transplanted with vaginal embolism and then injected with 4 mg PG; and those in the group III were first transplanted with vaginal embolism and then injected with 0.4 mg PG and 2.0 ml follicle-stimulating hormone (FSH). The rate of estrous synchronization, rate of embryo transfer and rejec- tion rate were compared between different treatments. [Result] The rates of estrous synchronization of the group I, II and III were 0%, 80% and 78%, respectively, and their rates of embryo transfer were 27.5%, 60.0% and 45.0%, respectively. [ Conclusion] Transplantation with vaginal embolism followed by injection with PG and FSH is the optimum method to induce estrus synchronization in yellow cattle.
基金Supported by the Fund for Visiting Scholars of the Ministry of Education of China(200809001)Fund for Young and Middle-aged Academic Leaders of "Qinglan Project" of Jiangsu Province(SJS[2010]No.27)
文摘With the enlargement of intensive feeding scale of dairy cows and improvement of milk yield, the incidence rate of displacement of abomasum in dairy cows increases gradually in recent years. Previous researches have shown that VFA in abomasums can significantly inhibit the shrinkage of smooth muscles of abomasums and lead to atony of abomasums, but the specific regulatory mechanisms are still unclear. In this study, the expression of VFA receptors GPR41 and GPR43 in cow abomasums were detected by PCR method, and the fragments were consistent with the excepted sizes. This study provided theoretical basis for the pathologic mechanism investigation, prevention and treatment of displacement of abomasum in dairy cows.