Exercise-with-melatonin therapy has complementary and synergistic effects on spinal cord injury and Alzheimer's disease,but its effect on stroke is still poorly understood.In this study,we established a rat model ...Exercise-with-melatonin therapy has complementary and synergistic effects on spinal cord injury and Alzheimer's disease,but its effect on stroke is still poorly understood.In this study,we established a rat model of ischemic stroke by occluding the middle cerebral artery for 60 minutes.We treated the rats with exercise and melatonin therapy for 7 consecutive days.Results showed that exercise-with-melatonin therapy significantly prolonged sleep duration in the model rats,increased delta power values,and regularized delta power rhythm.Additionally,exercise-with-melatonin therapy improved coordination,endurance,and grip strength,as well as learning and memory abilities.At the same time,it led to higher hippocampal CA1 neuron activity and postsynaptic density thickness and lower expression of glutamate receptor 2 than did exercise or melatonin therapy alone.These findings suggest that exercise-withmelatonin therapy can alleviate sleep disorder and motor dysfunction by increasing glutamate receptor 2 protein expression and regulating hippocampal CA1 synaptic plasticity.展开更多
Objective: The molecular mechanism of prostate cancer is poorly understood. The aim of the study was to investigate the prevalence and prognostic value of promoter hypermethylation of retinoic acid receptor beta (RARB...Objective: The molecular mechanism of prostate cancer is poorly understood. The aim of the study was to investigate the prevalence and prognostic value of promoter hypermethylation of retinoic acid receptor beta (RARB) and p16 among benign prostatic hyperplasia (BPH) and prostate cancer patients. Methods: In this case-control study, 63 patients were included in three groups; 21 with BPH as the control group, 21 with prostate cancer and good prognostic factors (based on prostate-specific antigen, Gleason score and stage) as good prognosis group, and 21 with prostate cancer and poor prognostic features as poor prognosis group. The prostate biopsy specimen of each individual was examined for hypermethylation of RARB and p16 promoters by methylation specific PCR (MSPCR). Results: Seven (33.3%) patients with good prognosis and 15 (71.4%) patients with poor prognosis were positive for RARB methylation, which were significantly higher than controls (P <0.0001). p16 promoter methylation was shown in 19.0% and 47.6% patients with good and poor prognosis, respectively. The RARB and p16 promoter methylation in the poor prognosis group was significantly higher than that in the good prognosis group (P =0.02 for RARB and P<0.0001 for p16). Conclusion: Hypermethylation of RARB and p16 promoters may predict prognosis in prostate cancer.展开更多
目的:探讨魄不安于肺不寐大鼠血清生物钟分子血清褪黑素(MT)、脑褪黑素相关受体(GPR50)和松果体的变化。方法:将24只SD大鼠随机分为模型组和正常组,每组12只。采用狭长多平台浅水环境法剥夺大鼠深睡眠42 d制备慢性睡眠剥夺模型。称取大...目的:探讨魄不安于肺不寐大鼠血清生物钟分子血清褪黑素(MT)、脑褪黑素相关受体(GPR50)和松果体的变化。方法:将24只SD大鼠随机分为模型组和正常组,每组12只。采用狭长多平台浅水环境法剥夺大鼠深睡眠42 d制备慢性睡眠剥夺模型。称取大鼠体质量,进行水迷宫检测、戊巴比妥钠诱导的睡眠实验和脑肌电监测,以E L I SA检测血清M T水平,以免疫组化法检测脑和脑干G P R50的水平,透射电子显微镜观察松果体的病理变化。结果:与正常组比较,模型组大鼠体质量显著减少(P<0.05,P<0.01)、上台潜伏期显著延长(P<0.05)、穿越原平台上方次数显著减少(P<0.05);戊巴比妥钠致睡眠潜伏期显著延长(P<0.05)、睡眠时间显著缩短(P<0.05);脑电图慢波睡眠第2期及快动眼睡眠比例显著减少(P<0.01);血清MT显著降低(P<0.05);脑及脑干GPR50表达显著升高(P<0.01,P<0.05);松果体超微结构出现明显病理变化。结论:狭长多平台浅水环境法可以剥夺大鼠部分深睡眠,引起大鼠记忆、睡眠行为改变,褪黑素和GPR50及松果体超微结构的变化。上述模型指标的变化可以用于研究魄不安于肺型不寐时参考。展开更多
基金supported by China Rehabilitation Research Center,No.2021zx-03the Special Fund for Joint Training of Doctoral Students between the University of Health and Rehabilitation Sciences and China Rehabilitation Research Center,No.2020 kfdx-008(both to TZ)。
文摘Exercise-with-melatonin therapy has complementary and synergistic effects on spinal cord injury and Alzheimer's disease,but its effect on stroke is still poorly understood.In this study,we established a rat model of ischemic stroke by occluding the middle cerebral artery for 60 minutes.We treated the rats with exercise and melatonin therapy for 7 consecutive days.Results showed that exercise-with-melatonin therapy significantly prolonged sleep duration in the model rats,increased delta power values,and regularized delta power rhythm.Additionally,exercise-with-melatonin therapy improved coordination,endurance,and grip strength,as well as learning and memory abilities.At the same time,it led to higher hippocampal CA1 neuron activity and postsynaptic density thickness and lower expression of glutamate receptor 2 than did exercise or melatonin therapy alone.These findings suggest that exercise-withmelatonin therapy can alleviate sleep disorder and motor dysfunction by increasing glutamate receptor 2 protein expression and regulating hippocampal CA1 synaptic plasticity.
文摘Objective: The molecular mechanism of prostate cancer is poorly understood. The aim of the study was to investigate the prevalence and prognostic value of promoter hypermethylation of retinoic acid receptor beta (RARB) and p16 among benign prostatic hyperplasia (BPH) and prostate cancer patients. Methods: In this case-control study, 63 patients were included in three groups; 21 with BPH as the control group, 21 with prostate cancer and good prognostic factors (based on prostate-specific antigen, Gleason score and stage) as good prognosis group, and 21 with prostate cancer and poor prognostic features as poor prognosis group. The prostate biopsy specimen of each individual was examined for hypermethylation of RARB and p16 promoters by methylation specific PCR (MSPCR). Results: Seven (33.3%) patients with good prognosis and 15 (71.4%) patients with poor prognosis were positive for RARB methylation, which were significantly higher than controls (P <0.0001). p16 promoter methylation was shown in 19.0% and 47.6% patients with good and poor prognosis, respectively. The RARB and p16 promoter methylation in the poor prognosis group was significantly higher than that in the good prognosis group (P =0.02 for RARB and P<0.0001 for p16). Conclusion: Hypermethylation of RARB and p16 promoters may predict prognosis in prostate cancer.
文摘目的:探讨魄不安于肺不寐大鼠血清生物钟分子血清褪黑素(MT)、脑褪黑素相关受体(GPR50)和松果体的变化。方法:将24只SD大鼠随机分为模型组和正常组,每组12只。采用狭长多平台浅水环境法剥夺大鼠深睡眠42 d制备慢性睡眠剥夺模型。称取大鼠体质量,进行水迷宫检测、戊巴比妥钠诱导的睡眠实验和脑肌电监测,以E L I SA检测血清M T水平,以免疫组化法检测脑和脑干G P R50的水平,透射电子显微镜观察松果体的病理变化。结果:与正常组比较,模型组大鼠体质量显著减少(P<0.05,P<0.01)、上台潜伏期显著延长(P<0.05)、穿越原平台上方次数显著减少(P<0.05);戊巴比妥钠致睡眠潜伏期显著延长(P<0.05)、睡眠时间显著缩短(P<0.05);脑电图慢波睡眠第2期及快动眼睡眠比例显著减少(P<0.01);血清MT显著降低(P<0.05);脑及脑干GPR50表达显著升高(P<0.01,P<0.05);松果体超微结构出现明显病理变化。结论:狭长多平台浅水环境法可以剥夺大鼠部分深睡眠,引起大鼠记忆、睡眠行为改变,褪黑素和GPR50及松果体超微结构的变化。上述模型指标的变化可以用于研究魄不安于肺型不寐时参考。
