BACKGROUND Dry eye syndrome(DES)after diabetic cataract surgery can seriously affect the patient’s quality of life.Therefore,effective alleviation of symptoms in patients with this disease has important clinical sign...BACKGROUND Dry eye syndrome(DES)after diabetic cataract surgery can seriously affect the patient’s quality of life.Therefore,effective alleviation of symptoms in patients with this disease has important clinical significance.AIM To explore the clinical effect of recombinant human epidermal growth factor(rhEGF)plus sodium hyaluronate(SH)eye drops on DES after cataract surgery in patients with diabetes.METHODS We retrospectively evaluated 82 patients with diabetes who experienced DES after cataract surgery at Tianjin Beichen Hospital,Affiliated Hospital of Nankai University between April 2021 and April 2023.They were classified into an observation group(42 cases,rhEGF+SH eye drops)and a control group(40 cases,SH eye drops alone),depending on the different treatment schemes.The therapeutic efficacy,dry eye symptom score,tear film breakup time(TFBUT),basic tear secretion score[assessed using Schirmer I test(SIt)],corneal fluorescein staining(FL)score,tear inflammatory markers,adverse reactions during treat-ment,and treatment satisfaction were compared between the two groups.RESULTS Therapeutic efficacy was higher in the observation group compared with the control group.Both groups showed improved TFBUT and dry eye,as well as improved SIt and FL scores after treatment,with a more pronounced improvement in the observation group.Although no marked differences in adverse reactions were observed between the two groups,treatment satisfaction was higher in the observation group.CONCLUSION rhEGF+SH eye drops rendered clinical benefits to patients by effectively ameliorating dry eye and visual impairment with favorable efficacy,fewer adverse reactions,and high safety levels.Thus,this treatment should be promoted in clinical practice.展开更多
AIM: Epidermal growth factor (EGF) plays an important rolein the regulation of gastrointestinal tissue growth anddevelopment, and it can stimulate epithelial proliferation,cell differentiation and growth. It has been ...AIM: Epidermal growth factor (EGF) plays an important rolein the regulation of gastrointestinal tissue growth anddevelopment, and it can stimulate epithelial proliferation,cell differentiation and growth. It has been established thatthe EGF can promote gastric cytoprotection and ulcerhealing. But the potential ability of EGF to regulate thegastric cancer growth is unknown. This study is toinvestigate the influence of EGF on human gastric cancercell and the implanted tumor growth of nude mice.METHODS: The cell growth rates of human gastricadenocarinoma cell lines MKN-28, MKN-45, SGC-7901 andnormal human gastric epithelial cells 3T3 were assessedwhen incubated with recombinant human EGF (rhEGF, 0.05, 0.1, 0.5, 1.0, 10, 50, 100 mg.L-1) using MTT method.The cells of MKN-28, MKN-45, SGC-7901 (gaatric cancertissue 1.5 mm3 ) were implanted in the BALB/cA nude micefor 10 days. The EGF was given intrapsritoneally (15, 30, 60μg. kg-1) for 3 weels. The body weights of the tumor-bearing animals and their tumor mass were measuredafterwards to assess the mitogenic effect of rhEGF in thenude mice.RESULTS: Within the concentration range of 0.05-100 mg.L-1 , rhEGF could increase the cell growth ofnormal 3T3 cells(cell growth rate 100 % vs 102.8 %, P＜0.05), but partiallyrestrain the gastric cancer cell growth. The latter effect wesrelated to cell differentiation. In 15-60μg/kg rhEGF groups,the mean implanted tumor mass of MKN-28 cell were 1.75 g,1.91 g, 2.08 g/NS group 1.97 g ( P＞ 0.05), the mean tumormass of SGC-7901 cell were 1.53 g, 1.07 g, 1.20 g/NS group1.07 g ( P ＞ 0.05), and for MKN-45 cell, the tumor masswere respectively 1.92 g, 1.29 g, 1.77 g/NS group 1.82 g( P＞ 0.05 ). So rhEGF had no obvious effect on implantedMKN-28, SGC-7901 and MKN-45 tumor growth.CONCLUSION: EGF has no stimulating effect on the humangastric cancer cell growth neither in vitro nor in vivo.展开更多
The human epidermal growth factor gene was synthesized with DNA synthesizer andby enzymes.Computer assisted to design the gene sequence and the restriction enzyme sites,andto elhninate the repeated and self-complement...The human epidermal growth factor gene was synthesized with DNA synthesizer andby enzymes.Computer assisted to design the gene sequence and the restriction enzyme sites,andto elhninate the repeated and self-complementary sequences.So that multiple doning and gene ex-pression were available for the designed gene sequence.The whole gene was formed by annealing8 chemically synthesized oligodeoxynucleotide fragments,enzymatically filling up the 4 twenty-basespaces and ligating the nicks,and was inserted into EcoR I and HInc Ⅱ sites ofplasmid pUC12.The recombinant plasmid was transformed into E.coli JM103 and 200 whitedones were obtained by X-gal and ampicilin screening.The positive clones were isolated andcharacterized by restriction enzyme analysis and DNA sequencing.展开更多
In this study the w/o/w extraction–evaporation technique was adopted to prepare poly(lactic-co-glycolic acid) (PLGA) microspheres loading recombinant human epidermal growth factor (rhEGF). The micro-spheres were char...In this study the w/o/w extraction–evaporation technique was adopted to prepare poly(lactic-co-glycolic acid) (PLGA) microspheres loading recombinant human epidermal growth factor (rhEGF). The micro-spheres were characterized for morphology by transmission electron microscopy (TEM) and particle size distribution. The release performances, the proliferation effects and therapeutic effects of rhEGF-loaded PLGA microspheres were all studied. The results showed that these spherical micro-spheres had a narrow size distribution and a high drug encapsulation efficiency (85.6%). RhEGF-loaded microspheres enhanced the growth rate of fibroblasts and wound healing more efficiently than pure rhEGF. The number of the proliferating cell nuclear antigen (PCNA) in the epidermis layer with the mi-crosphere treatment was significantly larger than those of the control groups. Overall locally sustained delivery of rhEGF from biodegradable PLGA microspheres may serve as a novel therapeutic strategy for diabetic ulcer repair.展开更多
AIM To study the transepithelial transport characteristics of the polyamine putrescine in human intestinal Caco-2 cell monolayers to elucidate the mechanisms of the putrescine intestinal absorption.METHODS The transep...AIM To study the transepithelial transport characteristics of the polyamine putrescine in human intestinal Caco-2 cell monolayers to elucidate the mechanisms of the putrescine intestinal absorption.METHODS The transepithelial transport and the cellular accumulation of putrescine was measured using Caco 2 cell monolayers grown on permeable filters.RESULTS Transepithelial transport of putrescine in physiological concentrations (>0.5 mM)from the apical to basolateral side was linear. Intracellular accumulation of putrescine was higher in confluent than in fully differentiated Caco-2 cells, but still negligible (less than 0.5%) of the overall transport across the monolayers in apical-to-basolateral direction. EGF enhanced putrescine accumulation in Caco-2 cells by four-fold, as well as putrescine conversion to spermidine and spermine by enhancing the activity of Sadenosylmethionine decarboxylase. However,EGF did not have any significant influence on putrescine flux across the Caco-2 cell monolayers. Excretion of putrescine from Caco-2cells into the basolateral medium did not exceed 50 picomoles, while putrescine passive flux from the apical to the basolateral chamber,contributed hundreds of micromoles polyamines to the basolateral chamber.CONCLUSION Transepithelial transport of putrescine across Caco-2 cell monolayers occurs in passive diffusion, and is not influenced when epithelial cells are stimulated to proliferate by a potent mitogen such as EGF.展开更多
基金Supported by Tianjin Health Research Project,No.TJWJ2023MS062。
文摘BACKGROUND Dry eye syndrome(DES)after diabetic cataract surgery can seriously affect the patient’s quality of life.Therefore,effective alleviation of symptoms in patients with this disease has important clinical significance.AIM To explore the clinical effect of recombinant human epidermal growth factor(rhEGF)plus sodium hyaluronate(SH)eye drops on DES after cataract surgery in patients with diabetes.METHODS We retrospectively evaluated 82 patients with diabetes who experienced DES after cataract surgery at Tianjin Beichen Hospital,Affiliated Hospital of Nankai University between April 2021 and April 2023.They were classified into an observation group(42 cases,rhEGF+SH eye drops)and a control group(40 cases,SH eye drops alone),depending on the different treatment schemes.The therapeutic efficacy,dry eye symptom score,tear film breakup time(TFBUT),basic tear secretion score[assessed using Schirmer I test(SIt)],corneal fluorescein staining(FL)score,tear inflammatory markers,adverse reactions during treat-ment,and treatment satisfaction were compared between the two groups.RESULTS Therapeutic efficacy was higher in the observation group compared with the control group.Both groups showed improved TFBUT and dry eye,as well as improved SIt and FL scores after treatment,with a more pronounced improvement in the observation group.Although no marked differences in adverse reactions were observed between the two groups,treatment satisfaction was higher in the observation group.CONCLUSION rhEGF+SH eye drops rendered clinical benefits to patients by effectively ameliorating dry eye and visual impairment with favorable efficacy,fewer adverse reactions,and high safety levels.Thus,this treatment should be promoted in clinical practice.
文摘AIM: Epidermal growth factor (EGF) plays an important rolein the regulation of gastrointestinal tissue growth anddevelopment, and it can stimulate epithelial proliferation,cell differentiation and growth. It has been established thatthe EGF can promote gastric cytoprotection and ulcerhealing. But the potential ability of EGF to regulate thegastric cancer growth is unknown. This study is toinvestigate the influence of EGF on human gastric cancercell and the implanted tumor growth of nude mice.METHODS: The cell growth rates of human gastricadenocarinoma cell lines MKN-28, MKN-45, SGC-7901 andnormal human gastric epithelial cells 3T3 were assessedwhen incubated with recombinant human EGF (rhEGF, 0.05, 0.1, 0.5, 1.0, 10, 50, 100 mg.L-1) using MTT method.The cells of MKN-28, MKN-45, SGC-7901 (gaatric cancertissue 1.5 mm3 ) were implanted in the BALB/cA nude micefor 10 days. The EGF was given intrapsritoneally (15, 30, 60μg. kg-1) for 3 weels. The body weights of the tumor-bearing animals and their tumor mass were measuredafterwards to assess the mitogenic effect of rhEGF in thenude mice.RESULTS: Within the concentration range of 0.05-100 mg.L-1 , rhEGF could increase the cell growth ofnormal 3T3 cells(cell growth rate 100 % vs 102.8 %, P＜0.05), but partiallyrestrain the gastric cancer cell growth. The latter effect wesrelated to cell differentiation. In 15-60μg/kg rhEGF groups,the mean implanted tumor mass of MKN-28 cell were 1.75 g,1.91 g, 2.08 g/NS group 1.97 g ( P＞ 0.05), the mean tumormass of SGC-7901 cell were 1.53 g, 1.07 g, 1.20 g/NS group1.07 g ( P ＞ 0.05), and for MKN-45 cell, the tumor masswere respectively 1.92 g, 1.29 g, 1.77 g/NS group 1.82 g( P＞ 0.05 ). So rhEGF had no obvious effect on implantedMKN-28, SGC-7901 and MKN-45 tumor growth.CONCLUSION: EGF has no stimulating effect on the humangastric cancer cell growth neither in vitro nor in vivo.
文摘The human epidermal growth factor gene was synthesized with DNA synthesizer andby enzymes.Computer assisted to design the gene sequence and the restriction enzyme sites,andto elhninate the repeated and self-complementary sequences.So that multiple doning and gene ex-pression were available for the designed gene sequence.The whole gene was formed by annealing8 chemically synthesized oligodeoxynucleotide fragments,enzymatically filling up the 4 twenty-basespaces and ligating the nicks,and was inserted into EcoR I and HInc Ⅱ sites ofplasmid pUC12.The recombinant plasmid was transformed into E.coli JM103 and 200 whitedones were obtained by X-gal and ampicilin screening.The positive clones were isolated andcharacterized by restriction enzyme analysis and DNA sequencing.
基金the National Natural Science Foundation of China (Grant No. 50373033)the Applied Foundational Research Key Fund of Tianjin (Grant No. 05YFJZJC01001)the International Cooperative Fund of Tianjin (Grant No. 05YFGHHZ20070)
文摘In this study the w/o/w extraction–evaporation technique was adopted to prepare poly(lactic-co-glycolic acid) (PLGA) microspheres loading recombinant human epidermal growth factor (rhEGF). The micro-spheres were characterized for morphology by transmission electron microscopy (TEM) and particle size distribution. The release performances, the proliferation effects and therapeutic effects of rhEGF-loaded PLGA microspheres were all studied. The results showed that these spherical micro-spheres had a narrow size distribution and a high drug encapsulation efficiency (85.6%). RhEGF-loaded microspheres enhanced the growth rate of fibroblasts and wound healing more efficiently than pure rhEGF. The number of the proliferating cell nuclear antigen (PCNA) in the epidermis layer with the mi-crosphere treatment was significantly larger than those of the control groups. Overall locally sustained delivery of rhEGF from biodegradable PLGA microspheres may serve as a novel therapeutic strategy for diabetic ulcer repair.
文摘AIM To study the transepithelial transport characteristics of the polyamine putrescine in human intestinal Caco-2 cell monolayers to elucidate the mechanisms of the putrescine intestinal absorption.METHODS The transepithelial transport and the cellular accumulation of putrescine was measured using Caco 2 cell monolayers grown on permeable filters.RESULTS Transepithelial transport of putrescine in physiological concentrations (>0.5 mM)from the apical to basolateral side was linear. Intracellular accumulation of putrescine was higher in confluent than in fully differentiated Caco-2 cells, but still negligible (less than 0.5%) of the overall transport across the monolayers in apical-to-basolateral direction. EGF enhanced putrescine accumulation in Caco-2 cells by four-fold, as well as putrescine conversion to spermidine and spermine by enhancing the activity of Sadenosylmethionine decarboxylase. However,EGF did not have any significant influence on putrescine flux across the Caco-2 cell monolayers. Excretion of putrescine from Caco-2cells into the basolateral medium did not exceed 50 picomoles, while putrescine passive flux from the apical to the basolateral chamber,contributed hundreds of micromoles polyamines to the basolateral chamber.CONCLUSION Transepithelial transport of putrescine across Caco-2 cell monolayers occurs in passive diffusion, and is not influenced when epithelial cells are stimulated to proliferate by a potent mitogen such as EGF.
