Objective To evaluate the effects of transforming growth factor β(TGF-β) and recombinant human bone morphogenetic protein 2 (rhBMP2) on human periodontal ligament fibroblasts (HPDLFs). Methods HPDLFs were done prima...Objective To evaluate the effects of transforming growth factor β(TGF-β) and recombinant human bone morphogenetic protein 2 (rhBMP2) on human periodontal ligament fibroblasts (HPDLFs). Methods HPDLFs were done primary culture to detect the distinct concentrations of TGF-P and rhBMF2 on its proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) synthesis and formation of the minerali-zed nodules, respectively. Results TGF-β (5～100ng/ml) significantly stimulated the proliferation of HPDLFs. The ALP activity of HPDLFs was evaluated evidently by 5ng/ml TGF-β. TGF-β( 0. 5 ～ 100ng/ml) had no effects on OC synthesis and formation of the mineralized nodules of HPDLFs. rhBMP2 (0. 25～2mg/ ml) had no remarkable effect on the proliferation of HPDLFs. The ALP activity, OC synthesis and forma-tion of the mineralized nodules of HPDLFs were significantly stimulated by 0. 5～ 2mg /ml rhBMP2. Conclusion The effects of TGF-β and rhBMP2 on HPDLFs are dose-dependent. TGF-P can stimulate HPDLFs to express the early marker of osteoblastic phenotype, and it lacks the ability to promote maturation of the osteogenic phenotype. rhBMP2 can not only stimulate the expression but also promote the maturation of osteoblas-tic phenotype of HPDLFs.展开更多
目的探讨不同浓度地塞米松(Dexamethasone,DEX)、重组人骨形态发生蛋白2(recombinant human bone morphogenetic protein 2,rhBMP2)及两者联合应用对体外培养的人牙髓细胞(human dental pulp cells,HDPCs)碱性磷酸酶(Alkaline Phosphata...目的探讨不同浓度地塞米松(Dexamethasone,DEX)、重组人骨形态发生蛋白2(recombinant human bone morphogenetic protein 2,rhBMP2)及两者联合应用对体外培养的人牙髓细胞(human dental pulp cells,HDPCs)碱性磷酸酶(Alkaline Phosphatase,ALP)活性的影响。方法组织块法培养HDPCs并进行鉴定;采用酶动力学的方法,观察DEX、rhBMP2及二者联合应用对HDPCs的ALP活性的影响。结果单独应用DEX时ALP活性明显增高,且在生理浓度范围内当浓度为0.01nmol/ml时,对HDPCs的ALP活性达到最大的刺激作用,rhBMP2对HDPCs的ALP活性呈浓度依赖性增强;当联合应用DEX和rhBMP2,ALP活性比单独应用相应浓度的rhBMP2明显增高。结论DEX、rhBMP2对HDPCs的ALP活性均有增强作用,同时二者对HDPCs的ALP活性有显著的功能放大性协同增强作用。展开更多
基金the National Natural Science Foundation of China (30000191), China Postdoctoral Science Foundation (1999- 17) and S
文摘Objective To evaluate the effects of transforming growth factor β(TGF-β) and recombinant human bone morphogenetic protein 2 (rhBMP2) on human periodontal ligament fibroblasts (HPDLFs). Methods HPDLFs were done primary culture to detect the distinct concentrations of TGF-P and rhBMF2 on its proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) synthesis and formation of the minerali-zed nodules, respectively. Results TGF-β (5～100ng/ml) significantly stimulated the proliferation of HPDLFs. The ALP activity of HPDLFs was evaluated evidently by 5ng/ml TGF-β. TGF-β( 0. 5 ～ 100ng/ml) had no effects on OC synthesis and formation of the mineralized nodules of HPDLFs. rhBMP2 (0. 25～2mg/ ml) had no remarkable effect on the proliferation of HPDLFs. The ALP activity, OC synthesis and forma-tion of the mineralized nodules of HPDLFs were significantly stimulated by 0. 5～ 2mg /ml rhBMP2. Conclusion The effects of TGF-β and rhBMP2 on HPDLFs are dose-dependent. TGF-P can stimulate HPDLFs to express the early marker of osteoblastic phenotype, and it lacks the ability to promote maturation of the osteogenic phenotype. rhBMP2 can not only stimulate the expression but also promote the maturation of osteoblas-tic phenotype of HPDLFs.
文摘目的探讨不同浓度地塞米松(Dexamethasone,DEX)、重组人骨形态发生蛋白2(recombinant human bone morphogenetic protein 2,rhBMP2)及两者联合应用对体外培养的人牙髓细胞(human dental pulp cells,HDPCs)碱性磷酸酶(Alkaline Phosphatase,ALP)活性的影响。方法组织块法培养HDPCs并进行鉴定;采用酶动力学的方法,观察DEX、rhBMP2及二者联合应用对HDPCs的ALP活性的影响。结果单独应用DEX时ALP活性明显增高,且在生理浓度范围内当浓度为0.01nmol/ml时,对HDPCs的ALP活性达到最大的刺激作用,rhBMP2对HDPCs的ALP活性呈浓度依赖性增强;当联合应用DEX和rhBMP2,ALP活性比单独应用相应浓度的rhBMP2明显增高。结论DEX、rhBMP2对HDPCs的ALP活性均有增强作用,同时二者对HDPCs的ALP活性有显著的功能放大性协同增强作用。
