Acute myocardial infarction （AMI） is an acute cardiovascular emergency. This study was undertaken to assess the effect of tumor necrosis factor-a （TNF-a） on ventricular arrhythmias induced byAMI in rats in vivo. ...Acute myocardial infarction （AMI） is an acute cardiovascular emergency. This study was undertaken to assess the effect of tumor necrosis factor-a （TNF-a） on ventricular arrhythmias induced byAMI in rats in vivo. Two hundred and forty male Wistar rats were randomized into a sham- operation group, an AMI group, and a recombinant human tumor necrosis factor receptor：Fc fusion protein（rhTNFR：Fc） group. Acute anterior wall myocardial infarction was produced in the AMI group by ligating the left anterior descending coronary artery （LAD）, and there was no ligation but operation in the sham-operation group. The rhTNFR：Fc group was treated with rhTNFR：Fc（10 mg/kg）, a TNF-a antagonist, 24 hours before LAD ligation. The spontaneous and induced programmed electrical stimulation ventricular arrhythmias were recorded at baseline and 10 minutes, 20 minutes, 30 minutes, 60 minutes, 3 hours, 6 hours and 12 hours after ligation. At the same time the protein and mRNA expression levels of TNF-a among different groups were detected by histochemistry and real-time fluorescent quantitative PCR. Expression of TNF-a increased markedly from 10 minutes after infarction, peaked at 20-30 minutes, and returned to baseline gradually in the AMI group and rhTNFR：Fc group. The time- windows of spontaneous and induced ventricular arrhythmias were similar. Compared with the AMI group, the rhTNFR：Fc group showed a lesser expression of TNF-a protein and a lower incidence of ventricular arrhythmias （P〈0.05）. There was no obvious change in the sham-operation group. The expression of TNF-a induced by AMI could contribute to the onset of ventricular arrhythmias.展开更多
NanoUPLC-ESI MS/MS was used to characterize the primary structure of recombinant human necrosis factor-αreceptorⅡ:IgG Fc fusion protein.The result indicated that the IgG Fc was IgG1 Fc but not others.This study is i...NanoUPLC-ESI MS/MS was used to characterize the primary structure of recombinant human necrosis factor-αreceptorⅡ:IgG Fc fusion protein.The result indicated that the IgG Fc was IgG1 Fc but not others.This study is important for this recombinant protein medicine to enter into international market.展开更多
目的:为了对重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白(rh TNFR:Fc)对医保预算的影响进行分析。方法:选取IMS Data 2015中的风湿类药品,把药品数量单位换算成限定日剂量(DDD),以IMS Data 2015 rh TNFR:Fc的用量为基线,假设其不同的新增...目的:为了对重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白(rh TNFR:Fc)对医保预算的影响进行分析。方法:选取IMS Data 2015中的风湿类药品,把药品数量单位换算成限定日剂量(DDD),以IMS Data 2015 rh TNFR:Fc的用量为基线,假设其不同的新增用量比例,按照90%和80%的报销比例分别计算新增的医保支出及其对风湿类药品和全部药品的医保支出的影响。全国医保支出数据来源于政府部门的统计资料。结果:按照90%的报销比例,当rh TNFR:Fc的新增用量为2015年用量的0~100%时,医保支出增加约为5.22~10.43亿元,风湿类药品医保支出增加63.44%~126.87%,全部药品医保支出增加0.04%~0.08%。按照80%的报销比例,其医保支出的增量则相应减少。结论:rh TNFR:Fc进入目录对风湿类药品医保支出的影响较大,但对全部药品医保支出的影响则很小。展开更多
文摘Acute myocardial infarction （AMI） is an acute cardiovascular emergency. This study was undertaken to assess the effect of tumor necrosis factor-a （TNF-a） on ventricular arrhythmias induced byAMI in rats in vivo. Two hundred and forty male Wistar rats were randomized into a sham- operation group, an AMI group, and a recombinant human tumor necrosis factor receptor：Fc fusion protein（rhTNFR：Fc） group. Acute anterior wall myocardial infarction was produced in the AMI group by ligating the left anterior descending coronary artery （LAD）, and there was no ligation but operation in the sham-operation group. The rhTNFR：Fc group was treated with rhTNFR：Fc（10 mg/kg）, a TNF-a antagonist, 24 hours before LAD ligation. The spontaneous and induced programmed electrical stimulation ventricular arrhythmias were recorded at baseline and 10 minutes, 20 minutes, 30 minutes, 60 minutes, 3 hours, 6 hours and 12 hours after ligation. At the same time the protein and mRNA expression levels of TNF-a among different groups were detected by histochemistry and real-time fluorescent quantitative PCR. Expression of TNF-a increased markedly from 10 minutes after infarction, peaked at 20-30 minutes, and returned to baseline gradually in the AMI group and rhTNFR：Fc group. The time- windows of spontaneous and induced ventricular arrhythmias were similar. Compared with the AMI group, the rhTNFR：Fc group showed a lesser expression of TNF-a protein and a lower incidence of ventricular arrhythmias （P〈0.05）. There was no obvious change in the sham-operation group. The expression of TNF-a induced by AMI could contribute to the onset of ventricular arrhythmias.
文摘NanoUPLC-ESI MS/MS was used to characterize the primary structure of recombinant human necrosis factor-αreceptorⅡ:IgG Fc fusion protein.The result indicated that the IgG Fc was IgG1 Fc but not others.This study is important for this recombinant protein medicine to enter into international market.
文摘目的:为了对重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白(rh TNFR:Fc)对医保预算的影响进行分析。方法:选取IMS Data 2015中的风湿类药品,把药品数量单位换算成限定日剂量(DDD),以IMS Data 2015 rh TNFR:Fc的用量为基线,假设其不同的新增用量比例,按照90%和80%的报销比例分别计算新增的医保支出及其对风湿类药品和全部药品的医保支出的影响。全国医保支出数据来源于政府部门的统计资料。结果:按照90%的报销比例,当rh TNFR:Fc的新增用量为2015年用量的0~100%时,医保支出增加约为5.22~10.43亿元,风湿类药品医保支出增加63.44%~126.87%,全部药品医保支出增加0.04%~0.08%。按照80%的报销比例,其医保支出的增量则相应减少。结论:rh TNFR:Fc进入目录对风湿类药品医保支出的影响较大,但对全部药品医保支出的影响则很小。
