Red-blood-cell-shaped chitosan microparticles with acid-triggered dissolution and auto-fluorescence were successfully fabricated by a simple strategy combining electrospraying with a solvent diffusion process controll...Red-blood-cell-shaped chitosan microparticles with acid-triggered dissolution and auto-fluorescence were successfully fabricated by a simple strategy combining electrospraying with a solvent diffusion process controlled by solvent evaporation. The sizes of the prepared chitosan microparticles were rela- tively uniform. Control of the solvent diffusion process was crucial for the formation of microparticles with concave morphology. A chitosan aqueous solution containing 20vo1% ethanol as the evaporable solvent and 30 vol% dimethyl sulfoxide as the diffusible solvent was optimal for preparation of chitosan microparticles with the desired red-blood-cell-like size and shape. These chitosan microparticles will be highly attractive for many biological and biomedical aoolications.展开更多
文摘Red-blood-cell-shaped chitosan microparticles with acid-triggered dissolution and auto-fluorescence were successfully fabricated by a simple strategy combining electrospraying with a solvent diffusion process controlled by solvent evaporation. The sizes of the prepared chitosan microparticles were rela- tively uniform. Control of the solvent diffusion process was crucial for the formation of microparticles with concave morphology. A chitosan aqueous solution containing 20vo1% ethanol as the evaporable solvent and 30 vol% dimethyl sulfoxide as the diffusible solvent was optimal for preparation of chitosan microparticles with the desired red-blood-cell-like size and shape. These chitosan microparticles will be highly attractive for many biological and biomedical aoolications.
文摘目的探索红细胞微粒(red blood cell microparticles,RMPs)对急性脑梗死(acute cerebral infarction,ACI)患者近期临床预后的影响。方法招募2015年12月-2018年3月就诊于邯郸市第一医院的ACI患者共168例,根据患者电子病历系统收集患者临床基线资料,入组后抽取静脉血进行实验室指标检测,同时出院后随访患者近期预后,根据预后情况将患者分为预后良好组(n=127)及预后不良组(n=41),比较两组患者临床特征差异性,应用多因素Logis⁃tic回归分析RMPs与ACI预后不良的相关性,通过受试者工作特征(ROC)曲线评估RMPs预测ACI预后不良的价值。结果本研究发现,预后不良组低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、超敏C反应蛋白(high-sensitivity C-reactive protein,Hs-CRP)、RMPs、D-二聚体及NIHSS评分高于预后良好组,同时多因素Logistic发现,RMPs、D-二聚体、美国国立卫生研究院卒中量表(National Institute of Health stroke scale,NIHSS)评分与ACI患者近期预后不良有相关性,其优势比(OR)(95%CI)分别为29.93(3.14~285.40)、3.55(1.36~9.27)及6.44(1.09~38.14);RMPs预测ACI患者近期预后不良的价值曲线下面积(AUC)(95%CI)为0.83(0.75~0.92),其最佳预测界值为51.36%,该界值下敏感性(95%CI)为0.85(0.78~0.91),特异性(95%CI)为0.53(0.49~0.62)。结论RMPs是预测ACI近期预后不良的指标,同时RMPs也是ACI患者预后不良的独立危险因素。
