Light quality response is a vital environmental cue regulating plant development. Conifers, like angiosperms, respond to the changes in light quality including the level of red (R) and far-red (FR) light, which follow...Light quality response is a vital environmental cue regulating plant development. Conifers, like angiosperms, respond to the changes in light quality including the level of red (R) and far-red (FR) light, which follows a latitudinal cline. R and FR wavelengths form a significant component of the entire plant life cycle, including the initial developmental stages such as seed germination, cotyledon expansion and hypocotyl elongation. With an aim to identify differentially expressed candidate genes, which would provide a clue regarding genes involved in the local adaptive response in Scots pine (Pinus sylvestris) with reference to red/far-red light;we performed a global expression analysis of Scots pine hypocotyls grown under two light treatments, continuous R (cR) and continuous FR (cFR) light;using Pinus taeda cDNA microarrays on bulked hypocotyl tissues from different individuals, which represented different genotypes. This experiment was performed with the seeds collected from northern part of Sweden (Ylinen, 68?N). Interestingly, gene expression pattern with reference to cryptochrome1, a blue light photoreceptor, was relatively high under cFR as compared to cR light treatment. Additionally, the microarray data analysis also revealed expression of 405 genes which was enhanced under cR light treatment;while the expression of 239 genes was enhanced under the cFR light treatment. Differentially expressed genes were re-annotated using Blast2GO tool. These results indicated that cR light acts as promoting factor whereas cFR antagonises the effect in most of the processes like C/N metabolism, photosynthesis and cell wall metabolism which is in accordance with former findings in Arabidopsis. We propose cryptochrome1 as a strong candidate gene to study the adaptive cline response under R and FR light in Scots pine as it shows a differential expression under the two light conditions.展开更多
In order to flower in the appropriate season,plants monitor light and temperature changes and alter downstream pathways that regulate florigen genes such as Arabidopsis(Arabidopsis thaliana)FLOWERING LOCUS T(FT).In Ar...In order to flower in the appropriate season,plants monitor light and temperature changes and alter downstream pathways that regulate florigen genes such as Arabidopsis(Arabidopsis thaliana)FLOWERING LOCUS T(FT).In Arabidopsis,FT messenger RNA levels peak in the morning and evening under natural long-day conditions(LDs).However,the regulatory mechanisms governing morning FT induction remain poorly understood.The morning FT peak is absent in typical laboratory LDs characterized by high red:far-red light(R:FR)ratios and constant temperatures.Here,we demonstrate that ZEITLUPE(ZTL)interacts with the FT repressors TARGET OF EATs(TOEs),thereby repressing morning FT expression in natural environments.Under LDs with simulated sunlight(R:FR=1.0)and daily temperature cycles,which are natural LD-mimicking environmental conditions,FT transcript levels in the ztl mutant were high specifically in the morning,a pattern that was mirrored in the toe1 toe2 double mutant.Low night-to-morning temperatures increased the inhibitory effect of ZTL on morning FT expression by increasing ZTL protein levels early in the morning.Far-red light counteracted ZTL activity by decreasing its abundance(possibly via phytochrome A(phyA))while increasing GIGANTEA(GI)levels and negatively affecting the formation of the ZTL-GI complex in the morning.Therefore,the phyA-mediated high-irradiance response and GI play pivotal roles in morning FT induction.Our findings suggest that the delicate balance between low temperature-mediated ZTL activity and the far-red light-mediated functions of phyA and GI offers plants flexibility in fine-tuning their flowering time by controlling FT expression in the morning.展开更多
In plant cultivation, the number of photons is more important than the light energy from the chemical reactions that occur during photosynthesis. In addition, the blue and red photon flux (B/R) ratio is an important p...In plant cultivation, the number of photons is more important than the light energy from the chemical reactions that occur during photosynthesis. In addition, the blue and red photon flux (B/R) ratio is an important parameter for plant cultivation. Here we discuss the effect of the spectral irradiance distribution and the B/R ratio on plant cultivation. We cultivated lettuce seedlings, Lactuca sativa L. Cv. Okayama, using a light-emitting diode illumination system that can precisely control the spectral irradiance distribution and B/R ratio. The B/R ratio varied from 0.36 to 2.06 according to the intensity of the blue light when the photosynthetic photon flux density values were sufficient to ensure the 150 - 200?μmol⋅m−2⋅s−1.?High photon flux densities of blue light result in reduced plant length, plant height, and leaf area, thereby suggesting its role in the suppression of leaf growth. Therefore, we conclude that a lower photon flux of blue light (B/R Ratio) is optimal for lettuce cultivation.展开更多
Phytochromes in seed plants are known to move into nuclei in a red light-dependent manner with or without interacting factors. Here, we show phytochrome relocation to the nuclear region in phytochrome-dependent Adiant...Phytochromes in seed plants are known to move into nuclei in a red light-dependent manner with or without interacting factors. Here, we show phytochrome relocation to the nuclear region in phytochrome-dependent Adiantum capillus-veneris spore germination by partial spore-irradiation experiments. The nuclear or non-nuclear region of imbibed spores was irradiated with a microbeam of red and/or far-red light and the localization of phytochrome involved in spore germination was estimated from the germination rate. The phytochrome for spore germination existed throughout whole spore under darkness after imbibition, but gradually migrated to the nuclear region following red light irradiation. In- tracellular distribution of PHY-GUS fusion proteins expressed in germinated spores by particle bombardment showed the migration of Acphy2, but not Acphyl, into nucleus in a red light-dependent manner, suggesting that Acphy2 is the photoreceptor for fern spore germination.展开更多
为了动态控制用于植物生长的人工光源，该文提出一种以光子数作为评价标准，使红蓝光比例连续可调的LED植物生长光源的配光方法。综合考虑红、蓝2种波段光源及其他光谱的作用，该文采用白光LED与红光LED 组合配比，以正向电流下 LED 的...为了动态控制用于植物生长的人工光源，该文提出一种以光子数作为评价标准，使红蓝光比例连续可调的LED植物生长光源的配光方法。综合考虑红、蓝2种波段光源及其他光谱的作用，该文采用白光LED与红光LED 组合配比，以正向电流下 LED 的光谱密度数据作为计算基础，提出配光设计算法，实现红蓝成分有效光子数维持一定的要求下，红光与蓝光光子数比在指定区间（4：1～9：1之间）连续可调，从而满足植物不同生长状态对光质成分的需要。展开更多
文摘Light quality response is a vital environmental cue regulating plant development. Conifers, like angiosperms, respond to the changes in light quality including the level of red (R) and far-red (FR) light, which follows a latitudinal cline. R and FR wavelengths form a significant component of the entire plant life cycle, including the initial developmental stages such as seed germination, cotyledon expansion and hypocotyl elongation. With an aim to identify differentially expressed candidate genes, which would provide a clue regarding genes involved in the local adaptive response in Scots pine (Pinus sylvestris) with reference to red/far-red light;we performed a global expression analysis of Scots pine hypocotyls grown under two light treatments, continuous R (cR) and continuous FR (cFR) light;using Pinus taeda cDNA microarrays on bulked hypocotyl tissues from different individuals, which represented different genotypes. This experiment was performed with the seeds collected from northern part of Sweden (Ylinen, 68?N). Interestingly, gene expression pattern with reference to cryptochrome1, a blue light photoreceptor, was relatively high under cFR as compared to cR light treatment. Additionally, the microarray data analysis also revealed expression of 405 genes which was enhanced under cR light treatment;while the expression of 239 genes was enhanced under the cFR light treatment. Differentially expressed genes were re-annotated using Blast2GO tool. These results indicated that cR light acts as promoting factor whereas cFR antagonises the effect in most of the processes like C/N metabolism, photosynthesis and cell wall metabolism which is in accordance with former findings in Arabidopsis. We propose cryptochrome1 as a strong candidate gene to study the adaptive cline response under R and FR light in Scots pine as it shows a differential expression under the two light conditions.
基金supported by the Japan Society for the Promotion of Science(JSPS)KAKENHI grant(No.19K16170 and No.23K05817 to A.K.)National Institutes of Health(NIH)(No.R01GM079712 to T.I.)the National Research Foundation(NRF)of Korea grant funded by the Korean Government(MSIT)(No.NRF-2020R1A2C1014655 andNo.NRF-2021R1A4A1032888 to Y.H.S.).
文摘In order to flower in the appropriate season,plants monitor light and temperature changes and alter downstream pathways that regulate florigen genes such as Arabidopsis(Arabidopsis thaliana)FLOWERING LOCUS T(FT).In Arabidopsis,FT messenger RNA levels peak in the morning and evening under natural long-day conditions(LDs).However,the regulatory mechanisms governing morning FT induction remain poorly understood.The morning FT peak is absent in typical laboratory LDs characterized by high red:far-red light(R:FR)ratios and constant temperatures.Here,we demonstrate that ZEITLUPE(ZTL)interacts with the FT repressors TARGET OF EATs(TOEs),thereby repressing morning FT expression in natural environments.Under LDs with simulated sunlight(R:FR=1.0)and daily temperature cycles,which are natural LD-mimicking environmental conditions,FT transcript levels in the ztl mutant were high specifically in the morning,a pattern that was mirrored in the toe1 toe2 double mutant.Low night-to-morning temperatures increased the inhibitory effect of ZTL on morning FT expression by increasing ZTL protein levels early in the morning.Far-red light counteracted ZTL activity by decreasing its abundance(possibly via phytochrome A(phyA))while increasing GIGANTEA(GI)levels and negatively affecting the formation of the ZTL-GI complex in the morning.Therefore,the phyA-mediated high-irradiance response and GI play pivotal roles in morning FT induction.Our findings suggest that the delicate balance between low temperature-mediated ZTL activity and the far-red light-mediated functions of phyA and GI offers plants flexibility in fine-tuning their flowering time by controlling FT expression in the morning.
文摘In plant cultivation, the number of photons is more important than the light energy from the chemical reactions that occur during photosynthesis. In addition, the blue and red photon flux (B/R) ratio is an important parameter for plant cultivation. Here we discuss the effect of the spectral irradiance distribution and the B/R ratio on plant cultivation. We cultivated lettuce seedlings, Lactuca sativa L. Cv. Okayama, using a light-emitting diode illumination system that can precisely control the spectral irradiance distribution and B/R ratio. The B/R ratio varied from 0.36 to 2.06 according to the intensity of the blue light when the photosynthetic photon flux density values were sufficient to ensure the 150 - 200?μmol⋅m−2⋅s−1.?High photon flux densities of blue light result in reduced plant length, plant height, and leaf area, thereby suggesting its role in the suppression of leaf growth. Therefore, we conclude that a lower photon flux of blue light (B/R Ratio) is optimal for lettuce cultivation.
文摘Phytochromes in seed plants are known to move into nuclei in a red light-dependent manner with or without interacting factors. Here, we show phytochrome relocation to the nuclear region in phytochrome-dependent Adiantum capillus-veneris spore germination by partial spore-irradiation experiments. The nuclear or non-nuclear region of imbibed spores was irradiated with a microbeam of red and/or far-red light and the localization of phytochrome involved in spore germination was estimated from the germination rate. The phytochrome for spore germination existed throughout whole spore under darkness after imbibition, but gradually migrated to the nuclear region following red light irradiation. In- tracellular distribution of PHY-GUS fusion proteins expressed in germinated spores by particle bombardment showed the migration of Acphy2, but not Acphyl, into nucleus in a red light-dependent manner, suggesting that Acphy2 is the photoreceptor for fern spore germination.
文摘为了动态控制用于植物生长的人工光源，该文提出一种以光子数作为评价标准，使红蓝光比例连续可调的LED植物生长光源的配光方法。综合考虑红、蓝2种波段光源及其他光谱的作用，该文采用白光LED与红光LED 组合配比，以正向电流下 LED 的光谱密度数据作为计算基础，提出配光设计算法，实现红蓝成分有效光子数维持一定的要求下，红光与蓝光光子数比在指定区间（4：1～9：1之间）连续可调，从而满足植物不同生长状态对光质成分的需要。
