In this work degradation of Acid Red-18(AR-18)was examined in the hydrodynamic cavitation reactor.Orifice plates with different holes geometry are used to determine the optimum plate to carry out the degradation based...In this work degradation of Acid Red-18(AR-18)was examined in the hydrodynamic cavitation reactor.Orifice plates with different holes geometry are used to determine the optimum plate to carry out the degradation based on cavitation number.The obtained optimum orifice plate is used as a cavitating device on varying parameters like initial AR-18 concentrations,pH,temperatures,and operating pressures of the reactor.A photocatalyst(TiO2)was prepared by the sol-gel method and used in combination with H2O2 to intensify the degradation of AR-18.The obtained optimum condition of hydrodynamic cavitation was again used in the ultrasonic cavitation reactor for the comparison.Hydrodynamic cavitation(orifice)given the highest degradation as compared to Hydrodynamic cavitation(Venturi)and Ultrasonic Cavitation with and without the use of TiO2.At TiO2(300 mg/L)dose,88.1%,70.4%and 64.8%degradation is obtained in HC-O,HC-V and UC reactor at initial AR-18 concentration(15 ppm),pH(3),Operating temperature(35C),and H2O2(300 mg/L).Hence the use of an advanced oxidation process can be successfully used with hydrodynamic cavitation to intensify the degradation of Acid Red-18 under the controlled operating parameters.展开更多
The nuclear-encoded small subunit ribosomal RNA gene (18S rDNA) of 16 isolates of Chondrus from 8 countries were sequenced. A total of 1796 nucleotides were obtained and aligned with the phylogenetic analysis conducte...The nuclear-encoded small subunit ribosomal RNA gene (18S rDNA) of 16 isolates of Chondrus from 8 countries were sequenced. A total of 1796 nucleotides were obtained and aligned with the phylogenetic analysis conducted. The results suggest that the entity from Dalian, China, regarded as C. sp1 is C. pinnulatus. The C. sp2 previously depicted as C. yendoi or Mazzaella japonica may belong to genus Chondrus. So, 4 Chondrus species, i.e. C. ocellatus, C. nipponicus, C. armatus, and C. pinnulatus are distributed in China. However, the entity from Connemara, Ireland, named C. crispus, is not a Chondrus species but that of Mastocarpus stellatus, although it is morphologically similar to C. crispus. Phylogenetic analysis based on complete 18S rDNA sequence data shows that genus Chondrus includes 3 main lineages: the Northern Pacific lineage, containing C. ocellatus, C. yendoi, and C. nipponicus; C. armatus, and C. pinnulatus form the sub-North Pacific lineage; and the Northern Atlantic Ocean lineage, comprising samples of C. crispus from Canada, Portugal, Ireland, Germany and France. The phylogenetic relationships indicate that genus Chondrus might have a North Pacific ancestral origin, radiated to North Atlantic area, and then formed the species C. crispus.展开更多
利用培养和免培养方法研究了一个云南红壤的真菌多样性。扩增的18S rRNA基因片段经过RsaⅠ、HinfⅠ、HaeⅢ三种限制性内切酶消化后,培养方法共获得16种限制性酶切长度多态类型(Restriction Fragment Length Polymorphism,RFLP),而免培...利用培养和免培养方法研究了一个云南红壤的真菌多样性。扩增的18S rRNA基因片段经过RsaⅠ、HinfⅠ、HaeⅢ三种限制性内切酶消化后,培养方法共获得16种限制性酶切长度多态类型(Restriction Fragment Length Polymorphism,RFLP),而免培养方法仅获得了12种RFLP类型。每个代表类型经DNA测序及系统发育分析表明,免培养方法得到的真菌全部属于子囊菌门。优势物种与Aspergillus niger类似,占免培养真菌的40.9%。其次是Penicillium属和Paecilomyces属真菌,分别占免培养真菌的18%和17%。此外,有13个克隆仅和环境克隆具有较近的亲缘关系。培养方法获取的真菌包括Ascomycota门(11.5%)、Zygomycota门(86.5%)以及Basidiomycota门(1.9%)真菌,优势克隆来自Mortierella属,占全部培养克隆的55.8%,有21.2%的序列与Absidiaglauca相似。两种方法观察到的真菌物种完全不同。展开更多
运用限制性片断长度多态性技术(Restriction Fragment Length Polymorphism,RFLP)的衍生技术PCR-RFLP,构建我国赤潮藻的特征酶切图谱,达到对赤潮藻进行区分的目的。主要内容包括两部分:(1)运用PCR方法设计引物并扩增出了9种赤潮藻的18S ...运用限制性片断长度多态性技术(Restriction Fragment Length Polymorphism,RFLP)的衍生技术PCR-RFLP,构建我国赤潮藻的特征酶切图谱,达到对赤潮藻进行区分的目的。主要内容包括两部分:(1)运用PCR方法设计引物并扩增出了9种赤潮藻的18S rDNA片段。(2)选择了TaqⅠ和PshBⅠ为实验用酶,并对9种赤潮藻的核糖体18S rDNA片段进行RFLP分析,RFLP结果显示,对9种赤潮藻区分明显。展开更多
文摘In this work degradation of Acid Red-18(AR-18)was examined in the hydrodynamic cavitation reactor.Orifice plates with different holes geometry are used to determine the optimum plate to carry out the degradation based on cavitation number.The obtained optimum orifice plate is used as a cavitating device on varying parameters like initial AR-18 concentrations,pH,temperatures,and operating pressures of the reactor.A photocatalyst(TiO2)was prepared by the sol-gel method and used in combination with H2O2 to intensify the degradation of AR-18.The obtained optimum condition of hydrodynamic cavitation was again used in the ultrasonic cavitation reactor for the comparison.Hydrodynamic cavitation(orifice)given the highest degradation as compared to Hydrodynamic cavitation(Venturi)and Ultrasonic Cavitation with and without the use of TiO2.At TiO2(300 mg/L)dose,88.1%,70.4%and 64.8%degradation is obtained in HC-O,HC-V and UC reactor at initial AR-18 concentration(15 ppm),pH(3),Operating temperature(35C),and H2O2(300 mg/L).Hence the use of an advanced oxidation process can be successfully used with hydrodynamic cavitation to intensify the degradation of Acid Red-18 under the controlled operating parameters.
基金Shandong Agriculture Seedstocks Project, and Knowledge Innovation Program of Chinese Academy of Sciences (kscx2-yw-n-47-02) the Key Laboratory of Experimental Marine Biology, Institute of Oceanology, CAS.
文摘The nuclear-encoded small subunit ribosomal RNA gene (18S rDNA) of 16 isolates of Chondrus from 8 countries were sequenced. A total of 1796 nucleotides were obtained and aligned with the phylogenetic analysis conducted. The results suggest that the entity from Dalian, China, regarded as C. sp1 is C. pinnulatus. The C. sp2 previously depicted as C. yendoi or Mazzaella japonica may belong to genus Chondrus. So, 4 Chondrus species, i.e. C. ocellatus, C. nipponicus, C. armatus, and C. pinnulatus are distributed in China. However, the entity from Connemara, Ireland, named C. crispus, is not a Chondrus species but that of Mastocarpus stellatus, although it is morphologically similar to C. crispus. Phylogenetic analysis based on complete 18S rDNA sequence data shows that genus Chondrus includes 3 main lineages: the Northern Pacific lineage, containing C. ocellatus, C. yendoi, and C. nipponicus; C. armatus, and C. pinnulatus form the sub-North Pacific lineage; and the Northern Atlantic Ocean lineage, comprising samples of C. crispus from Canada, Portugal, Ireland, Germany and France. The phylogenetic relationships indicate that genus Chondrus might have a North Pacific ancestral origin, radiated to North Atlantic area, and then formed the species C. crispus.
