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Biological and biochemical characterization of a red-eye mutant in Nilaparvata lugens (Hemiptera: Delphacidae) 被引量:3
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作者 Shu-Hua Liu Jing Yao +3 位作者 Hong-Wei Yao Peng-Ling Jiang Bao-Jun Yang Jian Tang 《Insect Science》 SCIE CAS CSCD 2014年第4期469-476,共8页
A red-eye colony was established in our laboratory in brown planthopper (BPH), Nilaparvata lugens (Stal), a major rice pest in Asia. Except for the red-eye phenotype, no other differences were observed between the... A red-eye colony was established in our laboratory in brown planthopper (BPH), Nilaparvata lugens (Stal), a major rice pest in Asia. Except for the red-eye phenotype, no other differences were observed between the wild-type (brown eye) and the mutant-type (red eye) in external characters. Genetic analysis revealed that the red-eye phenotype was controlled by a single autosomal recessive allele. Biological studies found that egg produc- tion and egg viability in the red-eye mutant colony were not significantly different from those in the wild-type BPH. Biochemical analysis and electronic microscopy examination revealed that the red-eye mutants contained decreased levels of both xanthommatin (brown) and pteridine (red) and reduced number of pigment granules. Thus, the changes of amount and ratio of the two pigments is the biochemical basis of this red-eye mutation. Our results indicate that the red-eye mutant gene (red) might be involved in one common gene locus shared by the two pigments in pigment transportation, pigment granule formation or some other processes. 展开更多
关键词 biological characterization eye pigment genetic analysis Nilaparvatalugens pigment granule red-eye mutant
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Cloning and Characterization of karmoisin Homologue Gene(Nlka) in Two Brown Planthopper Strains with Different Eye Colors 被引量:2
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作者 LIU Shu-hua TANG Jian +3 位作者 LUO Ju YANG Bao-jun WANG Ai-ying WU Jin-cai 《Rice science》 SCIE CSCD 2016年第2期104-110,共7页
The brown planthopper (BPH), Nilaparvata lugens, is a destructive insect pest of rice throughout Asia. Different from brown-eye color wild type, BPH also has red-eye color mutation phenotype. As a visible genetic ma... The brown planthopper (BPH), Nilaparvata lugens, is a destructive insect pest of rice throughout Asia. Different from brown-eye color wild type, BPH also has red-eye color mutation phenotype. As a visible genetic marker, the red-eye mutant in BPH is a valuable material. To reveal the eye color mutation mechanism, a karmoisin homologue gene (named as Nlka) was cloned from BPH. And karmoisin is always deemd as a xanthommatin-related gene in other insects, encoding phenoxazinone synthetase (PHS). Nlka is consisted of 7 exons and encodes a protein with 502 amino acids (NIKA). NIKA showed high amino acid identities with its insect homologues (48.8%-51.8%). Nlka transcripts can be detected at all the developmental stages and in all tissues tested, including egg, nymph, adult, body wall, ovary, fat body, midgut and Malpighian tubule. However, no constant In/Del or non-synonymous mutation was observed between the mutant and the wild type strains. Quantitative real-time PCR experiment also showed that Nlka transcript level had no significant differences between them. These results indicated that Nlka is not the target gene causing the red-eye color mutation phenotype of BPH. Through the second structure and motif analysis, the present study also showed that all the proteins deduced from the karmoisin genes in insects may be members of monocarboxylate transporters (MCTs) rather than PHSs. 展开更多
关键词 Nilaparvata lugens red-eye mutant karmoisin monocarboxylate transporter phenoxazinone synthetase rice gene clone
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