Evaluation of Uncertainty in Determination of Reducing Sugar Content in Sugarcane

[ Objective] This study aimed to establish an effective method for evaluating uncertainty in determination of reducing sugar content in sugarcane with Lane-Ernon method. [ Method] Based on analysis of the main sources of uncertainty in determination of reducing sugar content in sugarcane and mathematical model construction, combined uncertainty and expanded uncertainty were determined to establish the method for evaluation of uncertainty in determination. [ Result] Among uncertainties in determination of reducing sugar content in sugarcane with Lane-Ernon method, the greatest uncertainty was introduced by duplicate determination. According to results of statistical analysis, the expanded uncertainty in determination of reducing sugar content was 0.012%. [ Conclusion] This study provided theo- retical reference for evaluation of uncertainty in determination of reducing sugar content in sugarcane.

Effects of Plant Growth Regulators on the Contents of Reducing Sugar and Starch in Potato(Solanum tuberosum L.)

[Objective] The aim was to investigate the effects of plant growth regulators on contents of reducing sugar and starch in potato（Solanum tuberosum L.）.[Method]A potato cultivar named ＇Holand-212＇ with three treatments by spraying Diethyl aminoethyl hexanoate（DTA-6）,Uniconazole（S3307）and SOD mimics（SODM）were employed to compare differences of the contents of reducing sugar and starch in potato leaves,stolons and tubers in a field experiment.[Result]DTA-6 treatment could significantly reduce the contents of starch and reducing sugar in potato stolons in the period of 100-110 d after seeding,and it could also reduce the content of reducing sugar in tubers,but there is an opposite result in leaves in the same period,significantly.S3307 could extremely significantly increase the content of starch,and can promote the transfer of starch in stolons,in addition,it also could reduce the content of reducing sugar in tubers,and extremely significantly increase the content of starch in leaves at the 110 d after sowing.Moreover,there is a similar effect between SODM and S3307.[Conclusion]The research results are significant for understanding mechanisms of plant growth regulators and promoting application of plant growth regulator in potato.

Application of cellulase in producing reducing sugar with waste lignocellulosic

The cellulase expands the use of waste lignocellulosic and improves the feasibility of ethanol production with waste lignocellulosic. In this paper the types, mechanisms of cellulase and its application in reducing sugar production were presented in detail. The strains that produce cellulase and methods for improving the cellulase activity in reducing sugar production with waste lignocellulosic were described.

Reducing sugars production from pretreated rapeseed straw

Lignocellulosic biomass, being treated only with ammonia, treated with ammonia and then autoclaved, and treated with white-rot fungi after being mechanically chipped, were carried out to access the effects of lignocellulose degradation by white-rot fimgi cultured on rapeseed straw. Fourier transform infrared spectroscopic analysis was used to show that the white-rot fungus Bjerkandera sp. strain increase the susceptibility of straw to enzymatic saccharification by modifying the lignin component, revealing the effect of these pretreatments on enzymatic saccharification. Reducing sugar production from straws pretreated by ammonia/mechanical chipping/fungi degradation was 29.80% higher than the samples treated with ammonia/autoclaving, indicating an effective degradation of phenolic compounds. After ammonia pretreatment 41% of the straw was converted to RS （reducing sugars） （glucose 50%）. After 5 weeks pretreatment with the white-rot fungus, 54.8% of rapeseed straw was further converted to RS, 74% of which was glucose; while only 12% of the control straw was converted （glucose 42%）. The white-rot fungus Bjerkandera sp. strain degraded rapeseed straw preferentially at the early stage （before 20 d）, and the degradation selectivity was 0.181 1 （cellulose）, 0.364 1 （hemicellulose）, and 0.454 8 （lignin）, suggesting that removal of the phenolic barriers enhanced reducing sugar yield, and the efficiency of fungal pretreatment was comparable with that after alkali treatment, resulting a higher proportion of glucose in the hydrolysates.

Assessment of Physico-Chemical and Microbial Quality of Sugarcane Juice Sold by Street Vendors in Three Regions of Bangladesh

Sugar cane juice is a popular refreshing drink in most part of Bangladesh. It has great taste and health benefits;also it is available most of the public places at reasonable prices which consumed by road side customers including general public, shopping personals, tourists, students. In our country, street vendors crushing sugarcane between roller crusher and sold without any heat treatment or preservative, also served with or without added ice and lemon juice. The study aimed to identify and compare the physico-chemical and microbial quality of sugarcane juice. The chemical qualities of juices including moisture, PH, ash, total soluble solid, total sugar, reducing sugar and titrable acidity were found slightly different in Mymensingh, Gazipur and Narayangonj areas. The highest and lowest value of moisture, ash, PH, total soluble solids, total sugar, reducing sugar and titrable acidity were found in 84.33% - 79.26% (Gazipur-Mymensingh), 0.57% - 0.04% (Mymensingh-Gazipur), 5.9 - 2.9 (Gazipur-Narayangonj), 17.48% - 4.98% (Mymensingh-Narayangonj), 21.9% - 6.56% (Mymensingh-Gazipur), 3.7 - 2.1 (Gazipur-Mymensingh) and 0.523% - 0.007% (Narayangonj-Gazipur) respectively. For microbiological analysis, the total viable count of sugar cane juice in Mymensingh, Gazipur and Narayangonj were ranged from 0.6 × 105 - 43.6 × 105 cfu/ml, 4.6 × 105 - 21.6 × 105 cfu/ml and 3.6 × 105 - 36.6 × 105 cfu/ml respectively, where the permitted value is 1.0 × 104 cfu/ml, whereas the total coliform count was ranged from 0.4 × 105 - 6.4 × 105 cfu/ml, 0.6 × 105 - 8.4 × 105 cfu/ml and 0.00 - 8.4 × 105 cfu/ml, where the permitted value is 100 cfu/ml and total fungal count was ranged from 5.5 × 105 - 56.5 × 105 cfu/ml, 21.5 × 105 - 54.5 × 105 cfu/ml and 32.5 × 105 - 68.5 × 105 cfu/ml, where the permitted value is 1000 cfu/ml. According to the Gulf standard, the microbiological parameters of all the collected sugar cane juice were out of the permitted standards, so that a serious health outbreak can be caused anytime.

DETERMINATION OF THERMODYNAMIC AND KINETIC PARAMETERS OF LARGE SCALE CHROMATOGRAPHIC SEPARATION OF SUGAR AND REDUCING SUGAR

The parameter identification model of largr scale chromatography separation process is proposed. The Phase equilibrium constants and lumped mass transfer coefficients of sugar and reducing sugar adsorption on D1, D2 and D3 resins as well as the axial dispersion coefficients of the fluid through packed columns are determined by means of the pulse-response experiment technique with an inert substance as a tracer and the chromatography measuring technique. The elution curve calculated from these parameters is good agreement with the experimental elution curve. The sensitivity analysis of these parameters is carried out, and the ressult shows that the elation curves of chromatography separation are more sensitive to the variations Of the Phase equilibrium relationship than to the variation of the axial dispersion as well as the lumped mass transfer coefficients.

Research on the Influence of Acid Concentration on Reducing Sugar in Straw Waste

This paper studies the effect of reducing sugar concentration, reducing sugar yield and byproducts furfural concentration that generate by straw under the condition of acid concentration and flow rate in the gradient temperature hydrolysis process （60℃-230℃）, we can conclude that reducing sugar yield is highest when the concentration of sulfuric acid is 1%, the velocity of reaction liquid is 25mL/min, at the highest concentration of wheat straw yield of reducing sugar up to 32.9g/L, the reducing sugar yield was 60.8%. At this point the furfural concentration is lower than 1.0g/L, but in the entire gradient heating process, reducing sugar concentration peak of furfural concentration peak appears in the temperature lower, can realize the separation of the reducing sugar and furfural good, reduce the inhibition of fermentation by- products such follow-up treatment process.

Cascade Enzymatic Hydrolysis Coupling with Ultrafine Grinding Pretreatment for Sugarcane Bagasse Saccharification

The biorefinery process for sugarcane bagasse saccharification generally requires signifcant accessibility of cellulose. We reported a novel method of cascade cellulase enzymatic hydrol- ysis coupling with ultrafine grinding pretreatment for sugarcane bagasse saccharification. Three enzymatic hydrolysis modes including single cellulase enzymatic hydrolysis, mixed cellulase enzymatic hydrolysis, and cascade cellulase enzymatic hydrolysis were compared. The changes on the functional group and surface morphology of bagasse during cascade cellulase enzymatic hydrolysis were also examined by FT-IR and SEM respectively. The results showed that cascade enzymatic hydrolysis was the most efficient way to enhance the sugarcane bagasse saccharification. More than 65% of reducing sugar yield with 90.1% of glucose selectivity was achieved at 50 ℃, pH=4.8 for 72 h （1200 r/min） with cellulase I of 7.5 FPU/g substrate and cellulase II of 5 FPU/g substrate.

Physiological Responses of Yixing Lily Leaf and Bulb to Drought Stress

[Objective]To explore an effective method for evaluating drought resistance of Yixing lily and provide a reference for selecting drought-resistant Yixing lily varieties. [Method]Through artificial simulation of drought stress environment,the changes of physiological indexes,such as content of proline,malonaldehyde,and reducing sugar in Yixing lily leaf and bulb were observed,when soil water content changed. [Result]The response to drought stress of the Yixing lily leaf was greater than that of the bulb,and the content of the proline and malonaldehyde was higher than that of the reducing sugar. [Conclusion]The leaves as an appropriate material for evaluation and analysis on drought resistance can better reflect the physiological responses of Yixing lily to drought stress. And the content of the malonaldehyde and that of proline are preferable indexes.

Effects of Different Storage Temperature on Nutritional Quality of Potato Cultivar Kexin No.1

Objective] This study almed to investigate the effects of different storage temperature on nutritional quality of potato cuItivar Kexin No.1 and expIore the opti-mal storage temperature and time for Kexin No.1 with different uses. [Method] After storage at different temperature （1, 4, 10 and 16 ℃）, changes in dry matter, starch, reducing sugar and protein contents, amyIase activity and germination rate of potato cuItivar Kexin No.1 were observed and analyzed. [Result] Reducing sugar content of Kexin No.1 stored at 1 and 4 ℃ increased significantIy compared with that of Kexin No.1 stored at 10 and 16 ℃. Kexin No.1 stored at 1 ℃ exhibited no chiI ing injury. After storage at 16 ℃, Kexin No.1 began to germinate at 30 d, and the Iength and number of germinated buds increased significantIy with the extension of storage time, dispIaying a significant reduction in moisture content. Kexin No.1 potato couId be stored at 1 and 4 ℃ for more than 120 d without any changes in various indi-cators. Therefore, 1-4 ℃ was the optimal storage temperature for potato cuItivar Kexin No.1. [Conclusion] This study provided theoretical basis and technical support for the optimization of post-harvest storage conditions of potatoes.

Optimization of the Fermentation Conditions of Sweet Oats by Response Surface Methodology

Objective] The purpose of this study was to evaluate the effects of five variables （steaming time, moisture content before inoculation, inoculation amount, fer-mentation temperature, fermentation time） on the sweetness of sweet oats, obtained the best fermentation conditions. [Method] Plackett-Burman （PB） was to evaluate the effects of five variables, and selected significant factors. The steepest ascent was used to approach the optimal response surface experimental area. The optimal fer-mentation condition was obtained by central composite design and response surface analysis. [Results] It was indicated that moisture content, inoculation amount and fer-mentation temperature had significant influences on the content of the reducing sugars. The optimal conditions of moisture content, inoculation amount and temper-ature were 45.26%, 0.014%（g/g） and 28 ℃, respectively. The predicted value of the reducing sugar content was 13.16 mg/g. [Conclusion] Under the optimal conditions, the content of the reducing sugars in the sweet oats could be up to 12.91 mg/g, which was on the whole consistent with the predictive maximum value.

Effect of Rare Earth on Physiological-Chemical Characteristics and Its Growth of Aloe in Tissue Culture

In adopting tissue culture, effects of rare earth on Physiological and chemical characteristics, proliferation and rootage in aloe were studied. The results indicate that suitable concentration treatment of rare earth increases content of reducing sugar and protein, enhances activity intensity of superoxide dismutase (SOD), peroxidase (POD) isozymes and stripes of POD isozymes in the leaves. It also increases rate of proliferation and rootage respectively. The appropriate concentration of rare earth is 20～40 mg·L -1 in proliferation and rootage of aloe.

Effect of CaCl2, Citric Acid and Storage Period on Physico-Chemical Characteristics of Cherry Candy

Cherry cultivar Misri （Bigarreau Noir Grossa） was used for making candy. Pitting of fruit was done manually and KMS （0.2%） was added for bleaching. The fruit was pretreated using 4 levels of CaCl2 （Co = 0%, C1 = 1%, C2 = 1.5%, C3 = 2%） and stored in jars for 4 weeks. Thereafter, application of colour was done using Erythrosine （0.05%） followed by dipping pitted fruits in sugar syrup for 24 hours. The samples were treated with 4 levels of citric acid （To = 0%, Tl = 1%, T2 = 1.5%, T3 = 2%） and then concentrated subsequently till desired TSS of 70~ Brix was attained. The samples were dried in cabinet drier at 80 ±20 ℃till constant moisture was attained. The product was packed in polyethene pouches and stored under ambient storage conditions. Results revealed that there was gradual increase in reducing sugars （%）, total sugars （%） during storage while as moisture content （%） decreased. The product developed was found economically profitable and viable for commercial production.

Improvement of Cellulose Hydrolysis Process and Cost Savings

The hydrolysis process to obtain the so-called ＂reducing sugars＂ represents the main step involved in the production of the second generation bioethanol. This product can be obtained directly from various types of green biomass, replacing the use of cereals cultivations, with obvious benefits to the environment and the economy of agricultural production. However, it is necessary to improve the hydrolysis process of the cellulose to achieve this goal. To this purpose, we applied a chemical process formerly used. The values of sugars yield were increased by about 40% with respect to the previous study. Further significant cost savings were accomplished, resulting from the recovery of the by-product, calcium sulfate, commercially known as gypsum.

Flavor changes in Lentinus Edodes enzymatic hydrolysate maillard reaction products with different sugars by gas chromatography‐ion mobility spectrometry

The characteristic flavor of the enzymatic hydrolysate of Lentinus edodes,which was involved in the Maillard reaction by xylose,fructose,glucose,sucrose,mannose,maltose,vitamin C(VC)and L-arabinose,was determined and analyzed by gas chromatography-ion mobility spectroscopy(GC-IMS).The characteristic flavor fingerprints of Maillard reaction products(MRPs)of L.edodes enzymatic hydrolysate were constructed.The differences in the characteristic flavors of Maillard reaction with different reducing sugars and VC were compared.The main characteristic flavor,characteristic peak,characteristic marker substance and content were determined,a principal component analysis(PCA)of volatile organic compounds(VOCs)was carried out.The results showed that there were 42 kinds of monomers and some dimers of volatile compounds in the samples including 17 aldehydes,5 alcohols,7 ketones,2 esters,2 acids,7 other compounds and 2 ethers.The volatile VOCs of the sample with added sucrose,VC and L-arabinose were significantly higher than those of the enzymatic hydrolysate of L.edodes.After the Maillard reaction,the flavor of the hydrolysate was significantly improved,and the main components changed significantly.

Identification and Quantification of Monosaccharides in Aloe vera Gel by Acid and Enzymatic Hydrolysis and Wet Heat Treatment

Aloe vera (Aloe barbadensis Miller) possesses curative and therapeutic properties attributed to the polysaccharides found in its tissue. This work aimed to study hydrolyzed (chemical and enzymatical) Aloe vera gel extracts using ultrafiltration membranes with a pore size of 0.22 micron (μm). Nine chemical treatments were achieved with H2SO4 and three wet heat treatments were carried out at a pressure of 1.2 lbf/in2 for 15 minutes;in both cases, three different concentrations of Aloe vera gel juice (AGJ) were used: 1.5%, 2.5% and 3.5% w/w. The concentrations of H2SO4 were 0.10%, 0.25% and 0.40% w/w. Chemical experiments are performed over a factorial 32 design and results were analyzed using SPSS software (version 17, SPSS Inc.), finding the one labeled T7 (15 ml of AGJ 3.5% with 15 ml of H2SO4 0.10% added) the best of them, as it leaded to 0.0446 mg/ml of liberated glucose. Among the three wet heat treatments, the one labeled TC3 (15 ml of AGJ 3.5% with 15 ml of H2O added) was the best-performing one, as it leaded to 0.292 mg/ml of liberated glucose. Furthermore, an enzymatic hydrolysis was carried out using Novozymes’ Pectinex? AR and Viscozyme?. Hydrolisis with both enzymes yield to better results than acid hydrolysis: in the treatment with Pectinex? AR, 3.282 mg/ml of liberated glucose were obtained and 3.302 mg/ml in the treatment with Viscozyme?. The hydrolyzed substances obtained by acid and enzymatic hydrolysis, as well as by wet heat treatment, were subsequently analyzed by thin layer chromatography (TLC), using glucose, galactose and arabinose 1000 ppm solutions as reference patterns. Among the treatments by H2SO4, the one labeled T4 obtained an Rf value of 50, the same as on the galactose reference pattern.

Optimization of sugar production from Durian seeds via alkaline hydrolysis for second-generation bioethanol production

As one way to eliminate the issues found in the preceding generation,feedstock exploration in second-generation bioethanol production remains an issue,especially for a tropical country such as Indonesia.From exotic fruit by-products,durian holds a promising perspective that rests on its abundance,superb carbohydrate content and limited usage until now.This work presents the first-ever utilization of durian seeds for sugar production under optimized conditions through alkaline hydrolysis.A simple form of sugar was extracted by varying four parameters,namely substrate loading,NaOH concentration,hydrolysis time and hydrolysis temperature.Response surface methodology based on the Box-Behnken design was employed to outline the most optimum parameter values.Analysis of variance revealed that the quadratic model fit the data appropriately with the order of significance as substrate loading>hydrolysis time>NaOH concentration>hydrolysis temperature.The optimized conditions for reducing sugar yield,as high as 2.140 g/L,corresponded to<50 g/L substrate loading,0.522 M NaOH,60 minutes of hydrolysis time and 80oC hydrolysis temperature.The possible ethanol content of 1.094 g/L was also expected under optimized conditions,demonstrating great potential in second-generation bioethanol production.

Zebra chip disease incidence on potato is influenced by timing of potato psyllid infestation, but not by the host plants on which they were reared

The Zebra chip （ZC） syndrome is an emerging disease of potato and a major threat to the potato industry. The potato psyllid, Bactericerca cockerelli （Sulc） is believed to be a vector of the ZC pathogen, which is now thought to be Candidatus Liberibacter, a bacterium. To further understand the relationship between potato psyllid infestation and ZC disease expression, healthy potato plants at different growth stages （4, 6 and 10 weeks after germination） were exposed separately to potato psyllids that were separately reared on four solanaceous hosts plants （potato, tomato, eggplant or bell pepper） for more than 1 year. ZC symptoms, leaf rates and total nonstructural carbohydrate accumulation in leaves and tubers of healthy and psyllid-infested plants were monitored and recorded. Typical ZC symptoms were observed in leaves and tubers of all plants exposed to potato psyllids regardless of the host plant on which they were reared. This was also accompanied by significant reductions in net photosynthetic rate. Caged potato plants without exposure to potato psyllids （uninfested controls） did not show any ZC symptom in both foliage and in harvested tubers. Foliage damage and ZC expression were most severe in the potato plants that were exposed to potato psyllids 4 weeks after germination compared to plants infested at later growth stages. Tubers from potato psyllid-infested plants had significantly higher levels of reducing sugars （glucose） and lower levels of starch than those in healthy plants, indicating that potato psyllid infestation interfered with carbohydrate metabolism in either leaves or tubers, resulting in ZC expression.

Impact of double alkaline peroxide pretreatment on enzymatic hydrolysis of palm fibre

A double pretreatment was developed to improve the efficiency of enzymatic hydrolysis and to increase the sugars production from palm fibre in batch and fed-batch systems.After alkaline peroxide pretreatment using NaOH-H_(2)O_(2)solution,rigid structure of palm fibre was broken and partial of the hemicelluloses and lignin were removed.Compared with cellulase Accellerase^(■)1500,when using the conjunction of Accellerase^(■)1500 and Optimash BG,the conversion ratio increased from 35.9%to 45.0%and the reducing sugar yield increased from 245 mg/g substrate to 307 mg/g substrate.To further increase the hydrolysis efficiency,double pretreatment with NaOH-H_(2)O_(2)solution was used to treat the hydrolysized residues.Then,the hydrolysis ratio reached 93%and reducing sugar yield was 752 mg/g substrate.Fed-batch enzymatic hydrolysis was carried out with 7%(w/v)solids loading and 0.3 mL/g substrate.Then,7%solids were fed consecutively at 12 h,24 h,36 h and 48 h.After 144 h,the final concentration of reducing sugar reached 214.0 g/L and total biomass conversion ratio was 75.23%.