Lactic acid bacteria (LAB) were isolated from fresh sugar cane juice. It was found that several isolates exhibited a clear zone and growth on deMan, Rogosa, Sharpe (MRS) agar supplemented with sodium azide, bromocreso...Lactic acid bacteria (LAB) were isolated from fresh sugar cane juice. It was found that several isolates exhibited a clear zone and growth on deMan, Rogosa, Sharpe (MRS) agar supplemented with sodium azide, bromocresol purple and sucrose. However, only 17 isolates which formed large yellow areas were selected for further investigations. These isolates were further identified according to their morphological and biochemical characteristics. It was found that 10 of these isolates were homofermenters. One of these 10 isolates was selected for mutagenesis using chemical (Ethidium bromide) and physical (UV-B) mutagens followed by biochemical characterisation. A total of 112 mutants were isolated and 9 homofermentative isolates were further investigated for their ability to produce lactic acid. 1H-NMR spectroscopy confirmed that all mutant isolates produced lactic acid as the sole fermentation product.展开更多
In this work, sugar cane juice was fermented to produce polyhydroxyalkanoates （PHAs） by Alcaligenes latus TISTR 1403 and A. eutrophus TISTR 1095. The juice was characterized and composed of total sugars 105.5 g·...In this work, sugar cane juice was fermented to produce polyhydroxyalkanoates （PHAs） by Alcaligenes latus TISTR 1403 and A. eutrophus TISTR 1095. The juice was characterized and composed of total sugars 105.5 g·L^-1 （sucrose 36.6g·L^-1 , fructose 26.0g·L^-1 , glucose 21.8g·L^-1 and other sugars 21.1g·L^-1 ）. Each inoculums （ 10%, v/v） was separately cultivated in the medium containing 20g·L^-1 total sugars under condition （30℃, 200 rpm, pH 6.5-7）. It was found that the A. eutrophus can be grown better than the A. latus. Only the A. eutrophus was further cultured under different total sugar concentrations （20, 30, 40 and 50g·L^-1 ）. The optimal contents of total sugar, dry cell mass （DCM） and maximum PHAs were obtained at 50g·L^-1 , 6.013g·L^-1 and 1.84g·L^-1 , respectively after 60 h fermentation which were converted to biomass yield （Yx/s）, product yield （Yp/5）, specific product yield （Yp/x） and productivity of 0.163, 0.05, 0.306 and 0.031 g.Llhl. Large scale of PHAs production was conducted in 5 L fermentor using the optimal condition obtained under 30% dissolved oxygen. The DCM and the maximum PHAs were 5.881g·L^-1 and 1.281g·L^-1 which were calculated to values of Yx/s, Yp/s, Yp/x and productivity at 0.19, 0.04, 0.218 and 0.021g·L^-1 , respectively.展开更多
Sweet sorghum unlike grain sorghum has potential to accumulate sugars in stalks similar to sugarcane. Short duration and lower water requirements of sweet sorghum are other advantages over sugarcane. Sorghum is usuall...Sweet sorghum unlike grain sorghum has potential to accumulate sugars in stalks similar to sugarcane. Short duration and lower water requirements of sweet sorghum are other advantages over sugarcane. Sorghum is usually grown in kharif and rabi seasons. As an energy crop, industry demands supply of green cane as raw material through out the year. Hence this agronomic study was conducted to determine suitable time of planting of sweet sorghum under the agro climatic conditions prevailing in India, so that continuous supply of raw material is assured for factory operations through out the year, especially during the lean period of sugarcane crushing. Various physical and chemical characters of plant and stem juice were studied by taking up bimonthly plantings at four locations using 12 genotypes of sweet sorghum in the first year and quarterly plantings at four locations using 10 genotypes (8 genotypes common in both years) in the second year. Of the six plantings, planting during August and April were found to be not suitable for good sweet sorghum yields, therefore, in the second year, four plantings were taken up i.e., June, October, December and February at six locations. The various attributes selected for observations include cane yield, percent brix of stem juice, percent juice extractability, percent total soluble sugars and reducing sugars in the stem juice. Highest green cane yield and percent juice extractability was observed in June planting in all genotypes followed by February, and December in both bimonthly and quarterly plantings. Juice extraction percentage was also highest in June plantings followed by October, April and December plantings. June plantings recorded highest percent brix followed by December and February plantings in bimonthly plantings, while in quarterly planting, highest percent brix and total sugars in juice was observed in February planting followed by planting in June.展开更多
[目的]通过建立大鼠酒精性肝损伤病理模型,探讨甘蔗汁对大鼠酒精性肝损伤的保护作用。[方法]取大鼠40只随机分为4组,正常组、低剂量组、高剂组量和模型组。采用白酒灌胃的方法建立大鼠酒精性肝病模式,给予甘蔗汁进行保护。测定肝组织中...[目的]通过建立大鼠酒精性肝损伤病理模型,探讨甘蔗汁对大鼠酒精性肝损伤的保护作用。[方法]取大鼠40只随机分为4组,正常组、低剂量组、高剂组量和模型组。采用白酒灌胃的方法建立大鼠酒精性肝病模式,给予甘蔗汁进行保护。测定肝组织中超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量,并取肝组织制作石蜡切片,HE染色,普通光镜观察。[结果]甘蔗汁能够直接提高SOD活性、降低MDA含量,且低剂量(7.5 m l/kg)和高剂量(15 m l/kg)能够有效清除活性氧和自由基,明显改善肝组织脂肪样变。[结论]甘蔗汁能抑制酒精诱导脂质过氧化反应对肝组织的损伤,对酒精性肝损伤有明显的保护作用。展开更多
文摘Lactic acid bacteria (LAB) were isolated from fresh sugar cane juice. It was found that several isolates exhibited a clear zone and growth on deMan, Rogosa, Sharpe (MRS) agar supplemented with sodium azide, bromocresol purple and sucrose. However, only 17 isolates which formed large yellow areas were selected for further investigations. These isolates were further identified according to their morphological and biochemical characteristics. It was found that 10 of these isolates were homofermenters. One of these 10 isolates was selected for mutagenesis using chemical (Ethidium bromide) and physical (UV-B) mutagens followed by biochemical characterisation. A total of 112 mutants were isolated and 9 homofermentative isolates were further investigated for their ability to produce lactic acid. 1H-NMR spectroscopy confirmed that all mutant isolates produced lactic acid as the sole fermentation product.
文摘In this work, sugar cane juice was fermented to produce polyhydroxyalkanoates （PHAs） by Alcaligenes latus TISTR 1403 and A. eutrophus TISTR 1095. The juice was characterized and composed of total sugars 105.5 g·L^-1 （sucrose 36.6g·L^-1 , fructose 26.0g·L^-1 , glucose 21.8g·L^-1 and other sugars 21.1g·L^-1 ）. Each inoculums （ 10%, v/v） was separately cultivated in the medium containing 20g·L^-1 total sugars under condition （30℃, 200 rpm, pH 6.5-7）. It was found that the A. eutrophus can be grown better than the A. latus. Only the A. eutrophus was further cultured under different total sugar concentrations （20, 30, 40 and 50g·L^-1 ）. The optimal contents of total sugar, dry cell mass （DCM） and maximum PHAs were obtained at 50g·L^-1 , 6.013g·L^-1 and 1.84g·L^-1 , respectively after 60 h fermentation which were converted to biomass yield （Yx/s）, product yield （Yp/5）, specific product yield （Yp/x） and productivity of 0.163, 0.05, 0.306 and 0.031 g.Llhl. Large scale of PHAs production was conducted in 5 L fermentor using the optimal condition obtained under 30% dissolved oxygen. The DCM and the maximum PHAs were 5.881g·L^-1 and 1.281g·L^-1 which were calculated to values of Yx/s, Yp/s, Yp/x and productivity at 0.19, 0.04, 0.218 and 0.021g·L^-1 , respectively.
文摘Sweet sorghum unlike grain sorghum has potential to accumulate sugars in stalks similar to sugarcane. Short duration and lower water requirements of sweet sorghum are other advantages over sugarcane. Sorghum is usually grown in kharif and rabi seasons. As an energy crop, industry demands supply of green cane as raw material through out the year. Hence this agronomic study was conducted to determine suitable time of planting of sweet sorghum under the agro climatic conditions prevailing in India, so that continuous supply of raw material is assured for factory operations through out the year, especially during the lean period of sugarcane crushing. Various physical and chemical characters of plant and stem juice were studied by taking up bimonthly plantings at four locations using 12 genotypes of sweet sorghum in the first year and quarterly plantings at four locations using 10 genotypes (8 genotypes common in both years) in the second year. Of the six plantings, planting during August and April were found to be not suitable for good sweet sorghum yields, therefore, in the second year, four plantings were taken up i.e., June, October, December and February at six locations. The various attributes selected for observations include cane yield, percent brix of stem juice, percent juice extractability, percent total soluble sugars and reducing sugars in the stem juice. Highest green cane yield and percent juice extractability was observed in June planting in all genotypes followed by February, and December in both bimonthly and quarterly plantings. Juice extraction percentage was also highest in June plantings followed by October, April and December plantings. June plantings recorded highest percent brix followed by December and February plantings in bimonthly plantings, while in quarterly planting, highest percent brix and total sugars in juice was observed in February planting followed by planting in June.
文摘[目的]通过建立大鼠酒精性肝损伤病理模型,探讨甘蔗汁对大鼠酒精性肝损伤的保护作用。[方法]取大鼠40只随机分为4组,正常组、低剂量组、高剂组量和模型组。采用白酒灌胃的方法建立大鼠酒精性肝病模式,给予甘蔗汁进行保护。测定肝组织中超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量,并取肝组织制作石蜡切片,HE染色,普通光镜观察。[结果]甘蔗汁能够直接提高SOD活性、降低MDA含量,且低剂量(7.5 m l/kg)和高剂量(15 m l/kg)能够有效清除活性氧和自由基,明显改善肝组织脂肪样变。[结论]甘蔗汁能抑制酒精诱导脂质过氧化反应对肝组织的损伤,对酒精性肝损伤有明显的保护作用。
