Candidate reference measurement procedure for serum 17-hydroxyprogesterone quantification via isotope dilution liquid chromatography–tandem mass spectrometry

Background:Accurate quantification of 17-hydroxyprogesterone(17-OHP)in serum is vital for clinical and research applications.However,inter-laboratory variability in test results exists owing to the lack of a standardized reference measurement procedure(RMP).Therefore,in this study,we developed a highly accurate,cost-effective,and user-friendly candidate RMP(cRMP)for analyzing 17-OHP in serum.Methods:We quantified 17-OHP in serum using a one-step liquid–liquid extraction method with the addition of 17-OHP-^(13)C_(3),followed by liquid chromatography–tandem mass spectrometry.The ability of these methods to suppress interference was evaluated by chromatographic analysis.We assessed accuracy,specificity,the lower limit of quantitation,linearity,and matrix effects by following the standards specified by the Clinical and Laboratory Standards Institute.The consistency between the developed cRMP and the chemiluminescence method was evaluated through experiments with 120 clinical samples.Results:The developed cRMP required only 8 min for accurate quantification of serum 17-OHP without bias from matrix effects or interference from 19 metabolites added as potential interferents.The method exhibited favorable measurement performance,with a quantitation limit of 0.086 ng/mL,linear range of 0.1–400 ng/mL,a total imprecision of≤2.90%,spike recovery of 100.1%–100.6%,and relative deviations from assigned target values(RfB Institution)of−2.91%to 1.10%.The cRMP demonstrated good consistency with the conventional assay(chemiluminescence method),with a correlation coefficient R of 0.96977.Conclusion:A cRMP with high accuracy,cost-effectiveness,and convenient operation was developed for quantifying 17-OHP in serum.Its simplicity and robust performance make it an invaluable addition to the arsenal of analytical tools available for laboratories.This method can enhance the accuracy and reliability of 17-OHP measurements across various laboratories.

Hidden Dangers and Preventive Measures in the Inventory of Articles in Operating Room

Operating Room is an important place for surgical diagnosis and treatment and rescue of patients. With the rapid development of medical technology, high, precision, sharp continuous development, operating room safety management is particularly important. The error of the counting of the surgical articles causes the foreign body left in the patient’s body, which will cause irreversible damage to the patient and even cause the medical dispute and touch the legal problem. Operation items inventory system is an important working system in the operating room. Scientific and standardized methods and systems for counting surgical instruments and dressings during operation can prevent foreign bodies from being left in the body, prevent errors and accidents in the operating room, and are very important for ensuring the safety of the operation of patients. In order to ensure the safety of patients, it is necessary to ensure the correct inventory of surgical items. The factors affecting the accuracy of item counting in the operating room and the preventive measures are summarized as follows.