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Study of Hemorrhoid Disease in the Department of General Surgery of the Cs Ref of the Commune I Bamako
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作者 Tounkara Cheickna Diarra Issaka +8 位作者 Sanogo Modibo Togola Modibo Camara Ladji Soma Dembele Bakary Tientigui Traore Alhassane Togo Adégné Pierre Kante Lassana Ouattara Zanafon Diallo Gangaly 《Surgical Science》 2024年第2期36-47,共12页
This work was a retrospective prospective study carried out in the general surgery department of the Cs ref of commune I from January 2009 to December 2012. Our study aimed to determine the hospital frequency of hemor... This work was a retrospective prospective study carried out in the general surgery department of the Cs ref of commune I from January 2009 to December 2012. Our study aimed to determine the hospital frequency of hemorrhoidal disease, to identify the contributing factors, describe the clinical and therapeutic aspects and analyze the surgical consequences. We collected 73 patients with an average age of 37.8 years (range: 19 years and 77 years) with a sex ratio of 2.5 in favor of men. We operated on 49 patients or 67.1% of cases. 71.2% of our patients were married and lived in Bamako. Schoolchildren and middle managers made up 39.8% of cases. 82.2% of patients were seen in ordinary consultation and 69.9% complained of progressive anal pain in 66.7% of cases. This pain was triggered by defecation in 88.2% of our patients. The contributing factors were dominated by constipation (53.4%), working in a seated position (41.1%) and a sedentary lifestyle (16.4%) of cases. Stage IV external hemorrhoids and hemorrhoidal thrombosis represented 53.8% of surgical indications. The most used surgical technique was that of Milligan-Morgan, i.e. 51.9% of cases and 45.2% of patients treated medically received a combination of transit regulator, venotonics and analgesics. The surgical aftermath was marked by hemorrhage (3 cases), delayed healing (5 cases), urinary retention (1 case) and scarring anal stenosis (1 case). The morbidity rate was 5.48% of cases and a zero mortality rate. The average length of hospitalization was 2 days with extremes of 1 and 5 days. 展开更多
关键词 HEMORRHOID Surgery Cs ref C I BAMAKO MALI
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Perception and Management of Hemorroid Disease at the CSREF of the Commune Bamako Mali
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作者 Tounkara Cheickna Diarra Mohamed Hassimy +6 位作者 Diarra Issaka Sanogo Keita Bakary Dembele Bakary Tientigui Traore Alhassane Togo Pierre Adégné Lassana Kante 《Surgical Science》 2024年第2期89-97,共9页
This work was a prospective transversal study over 6 (six) months from May 2 to December 31, 2017 at the Reference Health Center of Commune I and the Chérif la Confessional Health Center in Bamako. The aim was to... This work was a prospective transversal study over 6 (six) months from May 2 to December 31, 2017 at the Reference Health Center of Commune I and the Chérif la Confessional Health Center in Bamako. The aim was to study the knowledge, attitudes and behavioral practices linked to hemorrhoids in Commune I of the Bamako district, to evaluate the frequency of hemorrhoidal disease, to determine the knowledge, attitudes and behavioral practices linked to hemorrhoids among patients, their companions and traditional therapists in Commune I of the district of Bamako and the factors which influence the motivations and decisions to resort to medical-surgical care and traditional medicine in patients suffering from hemorrhoids in order to analyze the results of the management of hemorrhoidal disease at Cs Ref CI. We collected 36 patients with hemorrhoidal disease, 25 caregivers and 15 traditional hemorrhoidal disease therapists. The sex ratio was 1.25 for patients;6.5 for traditional therapists and 1.5 for accompanying people. The average age of the patients was 32.75 years;49 years for traditional therapists and 28.76 years for those accompanying them. 55.6% of patients claim to know about hemorrhoidal disease;100% of traditional therapists and 80% of accompanying people. The dietary factor of hemorrhoidal disease was mentioned by 90% of patients;66.7% of traditional therapists and 100% of caregivers. Most patients initially resorted to traditional treatment out of fear of surgery and its after-effects. The patients were treated medically and surgically. 展开更多
关键词 Hemorrhoidal Disease CAREGIVERS Traditional Therapists Patients Surgery CS ref CI
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Development of High Accurate Family-use Digital Refractometer Based on CMOS
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作者 WANG Zhenxing JIA Zhenyuan 《Instrumentation》 2023年第3期12-22,共11页
This study aims to develop a low-cost refractometer for measuring the sucrose content of fruit juice,which is an important factor affecting human health.While laboratory-grade refractometers are expensive and unsuitab... This study aims to develop a low-cost refractometer for measuring the sucrose content of fruit juice,which is an important factor affecting human health.While laboratory-grade refractometers are expensive and unsuitable for personal use,existing low-cost commercial options lack stability and accuracy.To address this gap,we propose a refractometer that replaces the expensive CCD sensor and light source with a conventional LED and a reasonably priced CMOS sensor.By analyzing the output waveform pattern of the CMOS sensor,we achieve high precision with a personal-use-appropriate accuracy of 0.1%.We tested the proposed refractometer by conducting 100 repeated measurements on various fruit juice samples,and the results demonstrate its reliability and consistency.Running on a 48 MHz ARM processor,the algorithm can acquire data within 0.2 seconds.Our low-cost refractometer is suitable for personal health management and small-scale production,providing an affordable and reliable method for measuring sucrose concentration in fruit juice.It improves upon the existing low-cost options by offering better stability and accuracy.This accessible tool has potential applications in optimizing the sucrose content of fruit juice for better health and quality control. 展开更多
关键词 Brix Meter High-precision refractometer OVERSAMPLING 1D CMOS Sensor
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Clinical and Therapeutic Aspects of Inguinal Hernia in Children in the General Surgery Department of Reference Health Center in Commune I of Bamako Mali
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作者 Tounkara Cheickna Samake Hamidou +8 位作者 Simpara Mama Diarra Issaka Sanogo Modibo Sidibe Souleymane Togola Modibo Dembele Bakary Tientigui Coulibaly Yacaria Togo Pierre Adégné Kante Lassana 《Surgical Science》 2024年第2期54-63,共10页
Inguinal hernia in children is a congenital pathology in children linked to the persistence of the peritoneo-vaginal canal in children and the NüCK canal in girls;persisting into adulthood. This work aimed to stu... Inguinal hernia in children is a congenital pathology in children linked to the persistence of the peritoneo-vaginal canal in children and the NüCK canal in girls;persisting into adulthood. This work aimed to study inguinal hernia in children in the general surgery department of the Reference Health Center of Commune I of the Bamako District;to determine the frequency of inguinal hernia;describe the epidemiological, clinical and therapeutic aspects of inguinal hernias and in order to evaluate the cost of treatment. This was a prospective study from April 2017 to March 2018 during which 60 children with an inguinal hernia were collected. Inguinal hernias in children represented 9.23% of patients operated on in the department. The average age was 59.23 months with extremes of one month and 180 months and a standard deviation of 49.23 years. The male sex was dominant with a sex ratio equal to 9:1. The notion of prematurity was found in 11.7% and was associated with a testicle not in place in 1.7% of cases. 41.7% of our patients were referred by a doctor and 66.7% of patients presented with painless, intermittent inguino-scrotal swelling (83.3% of cases). The hernia was discovered before one week of life in 50% of cases. Inguinal swelling was absent in 11.7% but observed intraoperatively. The inguinal hernia was unilateral in 91.7% of cases and 70% of hernias were discovered by the parents during pushing efforts. The inguinal hernia was located on the right in 61.7% of cases. The hernia swelling was soft, painless, impulsive and reducible in 78.3% of cases. The swelling was inguino-scrotal in 58.3% of cases. The hernia was simple in 95% of patients and hernial strangulation was observed in 3 cases or 5% of cases. 98.3% of patients were ASA I. The treatment was carried out openly in all our patients including closure of the vaginal peritoneal canal in 95% of cases under general anesthesia in 98.3% of cases. The morbidity rate was 8.4% (surgical site infection: 6.7% of cases and hematoma: 1.7%). The immediate consequences were simple in 96.6% of cases. No cases of recurrence occurred during the 6 months after the intervention. The average cost of care was estimated at 69,743 FCFA. 展开更多
关键词 Inguinal Hernia Child Surgery Cs ref C I Bamako Mali
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MS16E020 A/D转换芯片采用外部基准源REF5025和MAX6325的对比测试分析
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作者 李渊博 王凯 王凌伟 《机电信息》 2024年第9期21-23,27,共4页
针对MS16E020 A/D转换芯片,通过对其采用外部基准源REF5025和MAX6325进行对比测试,分析了两种基准源在性能、精度、稳定性等方面的优劣。测试结果表明,MAX6325基准源表现出更好的性能和稳定性,为工程师在选择外部基准源时提供了有价值... 针对MS16E020 A/D转换芯片,通过对其采用外部基准源REF5025和MAX6325进行对比测试,分析了两种基准源在性能、精度、稳定性等方面的优劣。测试结果表明,MAX6325基准源表现出更好的性能和稳定性,为工程师在选择外部基准源时提供了有价值的参考。 展开更多
关键词 A/D转换芯片 MS16E020 ref5025 MAX6325
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基于MW-REF算法的心肺复苏影响因素分析 被引量:1
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作者 张友坤 陈伟 +3 位作者 靳小静 孙洁 李瑞月 张瑛琪 《科学技术与工程》 北大核心 2023年第22期9543-9549,共7页
针对传统模型对心肺复苏结果预测准确率较低、模型可解释性较差,提出了一种基于多模型加权递归消除法(MW-REF)的心肺复苏结果预测模型,并在Shapley加法解释(Shapley additive explanation,SHAP)框架下分析影响心肺复苏结果的关键因素。... 针对传统模型对心肺复苏结果预测准确率较低、模型可解释性较差,提出了一种基于多模型加权递归消除法(MW-REF)的心肺复苏结果预测模型,并在Shapley加法解释(Shapley additive explanation,SHAP)框架下分析影响心肺复苏结果的关键因素。采用了随机森林、GBDT、XGBOOST作为基模型,将其特征重要性得分加权后使用递归消除法过滤特征并对3种及模型采用Voting进行模型融合,利用五折交叉验证下的准确率作为最终特征选择标准。最后对最终特征数据集下的融合模型进行可解释性分析。实验结果表明,与传统的递归特征消除算法对比,该模型提升了心肺复苏结果预测的准确率,模型预测结果具有可解释性,可为临床诊断提供辅助,提高诊断效率与心肺复苏成功率。 展开更多
关键词 多模型加权递归特征消除法 心肺复苏 模型融合 SHAP
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RNA结合蛋白Aly/REF输出因子通过上调Yes相关蛋白表达促进胃癌细胞的增殖和迁移
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作者 李睿 邵爽 +2 位作者 孟玮 金丹 郭纪伟 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2023年第4期622-630,共9页
胃癌是一种常见的发病率高、起病隐匿、侵袭性强的消化道恶性肿瘤,其发病机制错综复杂。RNA结合蛋白Aly/REF输出因子(RNAbinding protein Aly/REF export factor, ALYREF)作为一种RNA胞嘧啶的5-甲基化修饰(m~5C)后的结合蛋白质,在调控RN... 胃癌是一种常见的发病率高、起病隐匿、侵袭性强的消化道恶性肿瘤,其发病机制错综复杂。RNA结合蛋白Aly/REF输出因子(RNAbinding protein Aly/REF export factor, ALYREF)作为一种RNA胞嘧啶的5-甲基化修饰(m~5C)后的结合蛋白质,在调控RNA出核及稳定其表达的过程中具有关键作用;Yes相关蛋白(yes-associated protein, YAP)作为Hippo-YAP信号通路中的关键分子,调控各种恶性肿瘤的生长和转移。本文旨在探讨ALYREF与YAP在促进胃癌细胞增殖和迁移中是否存在相关性,并初步探索这两者之间的作用机制。本文在胃癌细胞AGS和MKN-45中运用RT-PCR和Western印迹方法检测相关基因表达;CCK8试验检测细胞增殖;划痕试验和Trans-well试验分别检测细胞迁移和侵袭。结果显示,过表达ALYREF后YAP的表达增加(P<0.05),并且促进细胞的增殖、迁移和上皮细胞-间充质转化(epithelial-mesenchymal transition, EMT)(P<0.05);靶向敲低YAP,细胞的增殖、迁移和EMT能力显著抑制(P<0.05);过表达ALYREF且靶向敲除YAP与单独过表达ALYREF相比,YAP的表达显著降低(P<0.05),且细胞的增殖、迁移和EMT能力同样得到抑制(P<0.05);靶向敲低ALYREF,细胞的增殖、克隆形成和侵袭能力显著抑制(P<0.01)。综上所述,ALYREF通过上调YAP表达进而促进胃癌细胞的增殖和迁移。 展开更多
关键词 胃癌 增殖和迁移 RNA结合蛋白Aly/ref输出因子 Yes相关蛋白
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Monitoring Passive Transfer of Immunity in Neonatal Calves by Measuring Levels of IgG in Blood Using Immunoassay Method and Refractometer Measures of Serum and Colostrum
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作者 Osman A. Hameed Hussam Mustafa +1 位作者 Abdul Fatah M. Ahmed Mohamed Khidr Taha 《Open Journal of Veterinary Medicine》 2019年第12期194-201,共8页
IgG Check calf test has been used in this study to identify if a failure of passive transfer occurs in neonatal calves by measuring the level of IgG in blood. An adequate level of IgG measured in all calves at 48 hour... IgG Check calf test has been used in this study to identify if a failure of passive transfer occurs in neonatal calves by measuring the level of IgG in blood. An adequate level of IgG measured in all calves at 48 hours, 7 days and 14 days after birth showed level of IgG 1000 mg/dl. This level indicated that they have good passive transfer of immunity. Also, in this study colostrum quality fed to calves was detected using Brix Refractometer. The colostrum is of high quality as it contains 50 mg/ml of IgG. This Brix refractometer can be used on the farm level to estimate colostrum IgG content and monitoring colostrum feeding practices. Colostrum with high quality IgG could provide calves with enough IgG to attain successful passive transfer of immunity. Brix and Obione refractometers provide simple, rapid method for estimating IgG concentration on calf serum, thus considered to be the most common method for determining passive transfer failure. 展开更多
关键词 COLOSTRUM Passive IMMUNITY Immunoglobulin G (IgG) Failure of Transfer IMMUNITY (FTPI) Radial Immunodiffusion (RID) Turbidimetric IMMUNOASSAY (TA) refractometer (ref) BRIX refractometer (Brix) Obione refractometer (Obione) IGG Check Calf IMMUNOASSAY High Quality COLOSTRUM Inadequate Transfer of Passive IMMUNITY (ITPI)
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基于ANCF的旋转超弹性厚壁REF振动特性分析
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作者 范博 王忠民 《振动与冲击》 EI CSCD 北大核心 2023年第20期245-252,共8页
为了探究子午线轮胎面内振动特性,建立了旋转超弹性厚壁REF模型。并在具有随动坐标系的绝对节点坐标法(absolute nodal coordinate formulation,ANCF)框架下,提出平面内旋转环扇单元,对旋转超弹性厚壁弹性基环模型(ring on the elastic ... 为了探究子午线轮胎面内振动特性,建立了旋转超弹性厚壁REF模型。并在具有随动坐标系的绝对节点坐标法(absolute nodal coordinate formulation,ANCF)框架下,提出平面内旋转环扇单元,对旋转超弹性厚壁弹性基环模型(ring on the elastic foundation,REF)进行离散。考虑到非线性本构关系和旋转运动中的广义惯性力,建立了该模型的非线性运动微分方程以及线性化运动微分方程。通过与现有文献中的试验结果进行对比,验证了上述模型的有效性,并分析了弹性基参数、环体厚度和旋转角速度对子午线轮胎固有特性的影响规律。 展开更多
关键词 绝对节点坐标法(ANCF) 旋转ANCF环扇单元 厚壁ref模型 超弹性材料
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Pascal Realization by Comb-Spectral-Interferometry Based Refractometer
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作者 杨丽君 李岩 《Chinese Physics Letters》 SCIE CAS CSCD 2018年第10期85-88,共4页
To break through the limitations of existing pressure standards, which rely on the gravity and toxic mercury,the national metrological institutes prefer a quantum-based pressure standard. Combining the ideal gas law w... To break through the limitations of existing pressure standards, which rely on the gravity and toxic mercury,the national metrological institutes prefer a quantum-based pressure standard. Combining the ideal gas law with helium refractivity measurement, we demonstrate a scheme for the realization of the pressure unit. The refractometer is based on a spectral interferometry with an optical frequency comb and a double-spaced vacuum cell. Through fast Fourier transform of the spectral interferograms of the two beams propagating inside and outside the vacuum cell, the helium refractivity can be obtained with a combined standard uncertainty u(n) of2.9 × 10-9. Moreover, the final u(n) is -8.7 × 10-6 in a measurement range of several megapascals(MPa). Our apparatus is compact, fast(15 ms for one single measurement) and easy to handle. Furthermore, the measurement uncertainty will be improved to-1 × 10-9 or lower if a VIPA-based spectrometer is used. The value of u(p) will thus increase to 3 × 10-6 or better in several MPa. 展开更多
关键词 Pascal Realization by Comb-Spectral-Interferometry Based refractometer
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非肌层浸润性膀胱癌组织中PKM2和ALYREF表达及与预后预测价值研究
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作者 朱军 沈欣 曹冬 《现代检验医学杂志》 CAS 2023年第5期110-114,154,共6页
目的 研究非肌层浸润性膀胱癌(non-muscle invasive bladder cancer,NMIBC)组织中丙酮酸激酶M2亚型(pyruvate kinase M2 subtype,PKM2),RNA结合蛋白Aly/REF输出因子(Aly/REF export factor,ALYREF)表达及预后意义。方法 选取自2017年2月... 目的 研究非肌层浸润性膀胱癌(non-muscle invasive bladder cancer,NMIBC)组织中丙酮酸激酶M2亚型(pyruvate kinase M2 subtype,PKM2),RNA结合蛋白Aly/REF输出因子(Aly/REF export factor,ALYREF)表达及预后意义。方法 选取自2017年2月~2020年2月期间南京中医药大学翰林学院附属医院收治的84例NMIBC患者为研究对象。免疫组织化学检测NMIBC和膀胱正常黏膜组织中PKM2和ALYREF蛋白表达。Spearman秩相关分析膀胱癌组织中PKM2和ALYREF蛋白表达相关性。Kaplan-Meier生存曲线分析(Log-Rank检验)PKM2和ALYREF蛋白对无进展生存(progress free survive, PFS)预后的影响。COX比例风险模型分析影响NMIBC患者PFS预后的因素。结果 NMIBC组织中PKM2(71.43%),ALYREF(73.81%)蛋白阳性率高于膀胱正常黏膜组织(11.90%,10.71%),差异具有统计学意义(χ^(2)=61.225,68.522,均P <0.05)。T1期、高级别NMIBC癌组织中PKM2(88.37%,90.32%),ALYREF(80.05%,93.55%)蛋白阳性率分别高于Ta/Tis期(53.66%,60.38%)、低级别癌组织(60.98%,62.26%),差异具有统计学意义(χ^(2)=12.393,8.594;6.524,9.903,均P <0.05)。PKM2阳性组和阴性组患者的三年总体PFS分别为48.33%(29/60),87.50%(21/24),PKM2阳性组患者三年累积PFS显著低于PKM2阴性组患者(Log-Rank testχ^(2)=10.040,P=0.002);ALYREF阳性组和阴性组三年总体PFS分别为48.39%(30/62),90.91%(20/22),ALYREF阳性组患者三年累积PFS显著低于阴性组患者(Log-Rank test χ^(2)=11.110,P=0.001)。肿瘤TNM分期T1期(HR=1.614,95%CI:1.227~2.214)、高级别(HR=1.921,95%CI:1.324~2.788),PKM2阳性(HR=1.837,95%CI:1.229~2.745),ALYREF阳性(HR=1.744,95%CI:1.245~2.443)是影响NMIBC患者PFS预后的独立危险因素。结论 NMIBC中PKM2和ALYREF表达升高,两者表达呈正相关,是影响NMIBC患者预后的独立因素。 展开更多
关键词 非肌层浸润膀胱癌 丙酮酸激酶M2亚型 RNA结合蛋白Aly/ref输出因子
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Inguinal Hernia in Adults: Diagnostic and Therapeutic Aspects at Cs Ref CI Bamako Mali
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作者 Cheickna Tounkara Amadou Maiga +9 位作者 Bessy Samake Boubacar Yoro Sidibe Tani Kone Samake Hamidou Kaloga Daye Bagayoko Oumar Amadou Malle Alhassane Traore Bakary Tientigui Dembele Pierre Adégné Togo Zanafon Ouattara 《Surgical Science》 2023年第7期507-516,共10页
The inguinal hernia is a very frequent surgical pathology in the world and it represents 95% of the hernias of the groin. This work aimed to determine the frequency of inguinal hernias, describe the clinical and thera... The inguinal hernia is a very frequent surgical pathology in the world and it represents 95% of the hernias of the groin. This work aimed to determine the frequency of inguinal hernias, describe the clinical and therapeutic aspects of inguinal hernias, describe the morbidity and mortality related to inguinal hernias and evaluate the cost of treatment. Our study was conducted from January 2010 to December 2010 in the general surgery department of the Reference Health Center of Commune I in the district of Bamako. We collected 130 cases of inguinal hernias with a sex ratio of 15.25 in favor of the male sex. The mean age was 48.02 years (standard deviation: 17.53 years). Strangulated inguinal hernias accounted for 4.9% of abdominal surgical emergencies and 14.6% of all operated inguinal hernias. More than half of the patients (82%) were operated under local anesthesia. Clinical signs were marked by painless or irreducibly painful inguinal swelling. The diagnosis was clinical in all patients. Some patients benefited from a minimum blood assessment (rhesus grouping, glycaemia, hemoglobin level and hematocrit). The Shouldice technique was performed in 68% of patients. One case of organ resection was performed, i.e. 0.8% of cases. Morbidity was 4.6% due to wall abscess;0.8% scrotal hematoma type. We have not recorded any recurrence or death. 展开更多
关键词 INGUINAL HERNIAS Surgery Cs ref CI Bamako (Mali)
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Acute Intestinal Occlusions at the Cs Ref of Commune I of Bamako
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作者 Tounkara Cheickna Samake Hamidou +7 位作者 Diarra Issaka Sanogo Modibo Traore Fousseyni Togola Modibo Traore Alhassane Dembele Bakary Tientigui Togo Pierre Adégné Kante Lassana 《Surgical Science》 2023年第12期748-757,共10页
Acute intestinal obstructions are defined as a complete and persistent cessation of materials and gases in a segment of the digestive tract. They constitute a medical-surgical emergency. Our work aimed to study acute ... Acute intestinal obstructions are defined as a complete and persistent cessation of materials and gases in a segment of the digestive tract. They constitute a medical-surgical emergency. Our work aimed to study acute intestinal obstructions, to determine the hospital frequency, to describe the aspects (epidemiological, clinical and therapeutic), to analyze the surgical consequences and to evaluate the cost of the management of obstructions. acute intestinal infections in the general surgery department of the reference health center of commune I of Bamako in Mali. Our retrospective, longitudinal and descriptive study took place from January 1, 2015 to December 31, 2019 in the general surgery department of the reference health center in commune I of Bamako. The average age was 47.72 years with extremes of 15 and 78 years and a standard deviation of 16.07. Our sex ratio (56 men/15 women) was 3.38. The clinical signs were dominated by abdominal pain (100%), vomiting (52.9%), cessation of materials and gases (25.4%) and meteorism (35.3%). The main etiologies found intraoperatively were strangulated hernia (54.9%), bands and/or adhesions (21.1%), sigmoid volvulus (12.7%), colorectal tumor (7%), small bowel volvulus (2.8%) and acute intestinal intussusception (1.4%). Hernia repair was the most performed surgical procedure, i.e. 54.9%. The overall mortality rate was 1.4%. 展开更多
关键词 Acute Intestinal Obstructions Surgery Cs ref CI BAMAKO MALI
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Acute Peritonitis at the Reference Health Center of Commune I of the District of Bamako: Epidemiological, Clinical and Therapeutic Aspects
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作者 Tounkara Cheickna Samake Hamidou +8 位作者 Maïga Amadou Cisse Alou Hamadoun Diarra Issaka Sanogo Modibo Togola Modibo Dembele Bakary Tientigui Traore Alhassane Togo Pierre Adegne Kante Lassana 《Surgical Science》 2023年第12期728-737,共10页
Acute peritonitis is most often secondary to perforation of the digestive organ and/or the spread of an intra-abdominal septic focus. The objectives of this work were to study acute peritonitis in the general surgery ... Acute peritonitis is most often secondary to perforation of the digestive organ and/or the spread of an intra-abdominal septic focus. The objectives of this work were to study acute peritonitis in the general surgery department of the Cs ref of commune I in Mali, to determine the frequency of peritonitis, to describe the epidemiological, clinical and therapeutic aspects in order to analyze the surgical consequences and to assess the additional cost of treating acute peritonitis. This study was prospective, descriptive, cross-sectional involving 40 patients received in the surgery department of the Cs ref of commune I for acute peritonitis from January 1, 2018 to December 31, 2018. There were 40 patients among whom 28 (70%) were men and 12 were women (30%), i.e. a sex ratio = 2.3. The average age was 25 years with extremes varying between 16 and 54 years and a standard deviation of 11.78. Abdominal pain was the main reason for consultation. Clinical examination alone made it possible to make the diagnosis in 75% of cases. Surgical treatment depended on the intraoperative etiology. The clinical diagnosis was supported by ASP and abdominal ultrasound;performed respectively in 10% and 90% of patients. Appendiceal peritonitis was the intraoperative diagnosis observed in 50% of cases. All our patients benefited from a peritoneal toilet with drainage. We noted a morbidity rate of 5% dominated by parietal suppuration. The average cost of care was 175,000 FCFA. 展开更多
关键词 Acute Peritonitis Epidemiology Diagnostic Therapy Surgical Emergencies Cs ref CI BAMAKO MALI
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晚期肺癌血清Ape1/Ref-1表达及其对阿帕替尼联合三线化疗疗效的预测价值
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作者 王祥 丁纪涛 刘洋 《实用癌症杂志》 2023年第9期1421-1424,共4页
目的探讨晚期肺癌血清Ape1/Ref-1的表达及其对阿帕替尼联合三线化疗疗效的预测价值。方法收集70例晚期肺癌患者病例资料。采用随机数字表法进行分组,即对照组和研究组,均35例。对照组采用常规三线化疗治疗。研究组在对照组的基础上再予... 目的探讨晚期肺癌血清Ape1/Ref-1的表达及其对阿帕替尼联合三线化疗疗效的预测价值。方法收集70例晚期肺癌患者病例资料。采用随机数字表法进行分组,即对照组和研究组,均35例。对照组采用常规三线化疗治疗。研究组在对照组的基础上再予以阿帕替尼药物治疗。结果晚期肺癌组织Ape1/Ref-1蛋白阳性表达(91.43%)显著高于癌旁组织(χ^(2)=33.600,P=0.000)。化疗前,两组晚期肺癌患者Ape1/Ref-1蛋白相对表达量比较,差异无统计学意义(P>0.05);化疗后,两组Ape1/Ref-1蛋白相对表达量均明显低于化疗前,进一步组间比较,研究组Ape1/Ref-1蛋白相对表达量明显低于对照组,差异具有统计学意义(P<0.05)。Ape1/Ref-1阴性组生存率(87.50%)显著高于Ape1/Ref-1阳性组(χ^(2)=3.949,P=0.047),研究组累积生存率(91.43%)显著高于对照组(χ^(2)=10.096,P=0.001)。结论Ape1/Ref-1在晚期肺癌组织中呈现高表达,其表达越低,阿帕替尼联合三线化疗疗效越好。 展开更多
关键词 APE1/ref-1 晚期肺癌 阿帕替尼 三线化疗 疗效预测
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应用酵母双杂交系统筛选橡胶延长因子REF的互作蛋白 被引量:7
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作者 曾日中 李先昆 +3 位作者 聂智毅 代龙军 黎瑜 段翠芳 《热带亚热带植物学报》 CAS CSCD 北大核心 2011年第5期400-406,共7页
利用酵母双杂交系统,以橡胶树(Hevea brasiliensis)橡胶延长因子基因REF的开放阅读框(ORF)构建无自激活性的诱饵表达载体pBD-GAL4-REF,并筛选以pAD-GAL4-2.1载体构建的橡胶树胶乳cDNA文库,对阳性克隆的cDNA插入片段进行测序及生物学功... 利用酵母双杂交系统,以橡胶树(Hevea brasiliensis)橡胶延长因子基因REF的开放阅读框(ORF)构建无自激活性的诱饵表达载体pBD-GAL4-REF,并筛选以pAD-GAL4-2.1载体构建的橡胶树胶乳cDNA文库,对阳性克隆的cDNA插入片段进行测序及生物学功能分析。通过酵母双杂交筛选,共获得5种可能与REF互作的候选蛋白质,它们分别为与诱饵蛋白REF高度同源的REF家族成员、小橡胶粒子蛋白(SRPP)、翻译控制肿瘤蛋白(TCTP)、激发子响应蛋白和泛素耦联酶E2,这表明橡胶延长因子REF除了与自身高度同源蛋白质可能存在相互作用之外,还可能与TCTP和激发子响应蛋白等其它蛋白质发生相互作用。这些结果有助于揭示橡胶粒子的生物学功能。 展开更多
关键词 橡胶树 橡胶延长因子ref 橡胶生物合成 酵母双杂交系统
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杜仲Ref基因密码子偏好性分析 被引量:7
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作者 刘慧敏 乌云塔娜 杜红岩 《中南林业科技大学学报》 CAS CSCD 北大核心 2016年第3期8-12,共5页
杜仲是世界上唯一的硬橡胶树种,是能够替代三叶橡胶的最具开发潜力的胶源植物。本研究以杜仲胶合成下游途径Ref基因为研究对象,运用Codon W和CUSPS等研究Ref基因的密码子组成特点及使用偏好性。研究结果表明,杜仲Ref基因的GC含量为49%,... 杜仲是世界上唯一的硬橡胶树种,是能够替代三叶橡胶的最具开发潜力的胶源植物。本研究以杜仲胶合成下游途径Ref基因为研究对象,运用Codon W和CUSPS等研究Ref基因的密码子组成特点及使用偏好性。研究结果表明,杜仲Ref基因的GC含量为49%,密码子第三位碱基的GC含量为59%;杜仲Ref基因有28个偏好使用的密码子,其中约60%(17个)的密码子以G/C结尾;与大肠杆菌、酵母、拟南芥和烟草的基因组密码子使用偏好性比较,杜仲与拟南芥基因组密码子的使用偏性更为接近,拟南芥更适合作为Ref转基因的受体。本研究为预测杜仲Ref基因的异源表达,适宜表达系统的选择以及Ref基因密码子的改造以提高其在宿主中的表达,进行有效的基因功能验证提供理论参考。 展开更多
关键词 杜仲 ref基因 密码子 偏好性
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红景天苷对脑缺血再灌注大鼠脑神经元APE/Ref-1的影响 被引量:10
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作者 刘晓梅 王淑英 刘斌 《中国老年学杂志》 CAS CSCD 北大核心 2009年第5期518-520,共3页
目的研究红景天苷对局灶性脑缺血/再灌注(I/R)损伤后神经可塑性和DNA修复酶APE/Ref表达的影响。方法Wistar大鼠随机分为假手术组,I/R模型组和红景天苷干预组,采用线栓法制造大鼠大脑局灶缺血(2h)/再灌注模型,于灌注后0.25、1、3、7d4个... 目的研究红景天苷对局灶性脑缺血/再灌注(I/R)损伤后神经可塑性和DNA修复酶APE/Ref表达的影响。方法Wistar大鼠随机分为假手术组,I/R模型组和红景天苷干预组,采用线栓法制造大鼠大脑局灶缺血(2h)/再灌注模型,于灌注后0.25、1、3、7d4个时间点断头取脑,HE和尼氏染色观察神经元形态学变化,免疫组化方法检测无嘌呤无嘧啶位点/氧化还原因子-1(APE/Ref-1)的表达。结果与其他两组比较,红景天苷组梗死灶范围明显减小,梗死灶周围皮质神经元损伤明显减轻(P<0.05);APE/Ref-1阳性细胞数表达显著增高(P<0.05)。结论红景天可以易化脑I/R损伤后中枢神经系统神经再生和修复的可塑性,抑制APE/Ref-1降低可能是其在I/R早期发挥神经保护作用的一条重要途径。 展开更多
关键词 红景天苷 脑缺血/再灌注 神经可塑性 APE/ref
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非学术影响评价:英国REF科研影响评估的创新性评析 被引量:9
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作者 茹宁 闫广芬 《国家教育行政学院学报》 CSSCI 北大核心 2020年第9期24-34,共11页
对科学研究所产生的社会价值与影响进行评价,正在成为世界各国科研评价机制改革的新趋势。英国在REF中引入“科研影响”评估,明确将非学术性质的社会影响作为评估对象。该评估采用了以定性为主、以定量为辅的混合评价方法,并借助对评估... 对科学研究所产生的社会价值与影响进行评价,正在成为世界各国科研评价机制改革的新趋势。英国在REF中引入“科研影响”评估,明确将非学术性质的社会影响作为评估对象。该评估采用了以定性为主、以定量为辅的混合评价方法,并借助对评估结果的“后分析”技术,形成了英国科研影响的全球可持续扩散机制。REF科研影响评估的诸多创新为我国开发高校社会服务与贡献评价机制提供了有益借鉴。 展开更多
关键词 ref 科研影响评估 非学术影响
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基于REF模式的上海市临床医生科研绩效评价 被引量:4
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作者 张文珊 王世婷 +1 位作者 张翔 贾仁兵 《解放军医院管理杂志》 2020年第10期913-917,共5页
目的构建科学、合理的公立临床医生科研绩效评价指标体系,打破以数量增长为导向的模式,着重体现科研质量。方法以英国REF模型为指标基础和内涵,综合运用文献研究法、德尔菲专家咨询法构建评价指标体系,利用层次分析法对专家评价数据进... 目的构建科学、合理的公立临床医生科研绩效评价指标体系,打破以数量增长为导向的模式,着重体现科研质量。方法以英国REF模型为指标基础和内涵,综合运用文献研究法、德尔菲专家咨询法构建评价指标体系,利用层次分析法对专家评价数据进行统计分析。结果构建基于以科研质量为导向、以科研未来发展为基础的绩效评价指标体系,并进行可行性的实证研究验证结果,重点突出不同类型临床医生的分类评价。结论构建的指标体系增加学科影响、科研环境和科研可持续性等指标,能有效评估同一学科人才和不同学科人才的科研绩效,有利于临床医生找到参照对标,形成良性竞争,也促进绩效评估的可操作性和可接受性。 展开更多
关键词 ref模式 科研绩效评价 公立医院 临床医生
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