Objectives.To study the expression of TNF α mRNA and the effect of somatostatin on the expression of TNF α mRNA in multiorgans of rats with acute hemorrhagic necrotic pancreatitis(AHNP). Methods.AHNP in the rat was ...Objectives.To study the expression of TNF α mRNA and the effect of somatostatin on the expression of TNF α mRNA in multiorgans of rats with acute hemorrhagic necrotic pancreatitis(AHNP). Methods.AHNP in the rat was induced by retrograde injection of 5% sodium taurocholate into the bile-pancreatic duct. Somatostatin octapeptide (SS-OP) (2μg/kg)was injected into the femoral vein imme- diately in rats of the treatment group after inductive AHNP. Rats of the sham operative group received in- jection of saline. Sixty animals of the AHNP and sham operative groups at the designated time(0. 2h, 0. 5 h, 2h, 4h, 8h, after the operation,six animals at each time point)and tweleve animals of treatment group at 4h after the operation were sacrificed for samples of pancreas, liver and lung. The expressions of TNF α mRNA within the pancreas, liver and lung were established by RT－PCR. Results. TNF α mRNA became detectable in the pancreas as early as 0. 2h after inductive AHNP, while it was undetectable in other organs until 0. 5h. Expression of TNF α mRNA in each tissue increased continuously and reached a peak at 4h,demonstrating a significant difference compared with that at 0. 5h and 8h. Expressions of TNF α mRNA from pancreas, liver and lung were decreased 50-80% in the treat- ment group, the pancreatic necrosis was also attenuated dramatically. Conclusion. TNF α mRNA was detectable in pancreas,liver and lung tissues at the early stage of AH- NP.SS-OP can significantly inhibit the expression of TNF α mRNA and attenuate the pancreatic necrosis. We feel that this may be an important mechanism of SS-OP in the treatment of AHNP.展开更多
Acute necrotising pancreatitis is characterized by inflammatory and necrotic events, which followthe initial intra-acinar injury involving enzyme activation, and disruption of the acinar cytoskeleton. At present, apo...Acute necrotising pancreatitis is characterized by inflammatory and necrotic events, which followthe initial intra-acinar injury involving enzyme activation, and disruption of the acinar cytoskeleton. At present, apoptosis has become a hot topic in many kinds of disease. Bax and Bcl-2 are the important control gene during cell apoptosis. 2 Previous study has shown that interleukin 10 （IL-10） is a kind of important antiinflammatory cytokine and plays a role of selfdefense mechanism,展开更多
目的:研究regⅠ在急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)大鼠肠组织中的表达,探讨该基因的表达与ANP肠黏膜损害的关系.方法:Sprague-Dawley大鼠随机分为假手术对照组(n=40)和ANP组(n=80).对照组开腹后只翻动胰腺,ANP组...目的:研究regⅠ在急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)大鼠肠组织中的表达,探讨该基因的表达与ANP肠黏膜损害的关系.方法:Sprague-Dawley大鼠随机分为假手术对照组(n=40)和ANP组(n=80).对照组开腹后只翻动胰腺,ANP组大鼠胰胆管恒速逆行注射30g/L牛磺胆酸钠,制成ANP大鼠模型.观察胰腺和小肠的病理改变及小肠黏膜通透性的改变,应用逆转录聚合酶链反应(RT-PCR)检测胰腺、小肠组织中regⅠ mRNA的表达水平,并研究所观察指标与regⅠ基因表达的相关性.结果:ANP组大鼠术后12,24和36h肠黏膜组织病理学评分明显高于对照组(1.8±0.89 vs 0.2±0.42,3.3±1.17 vs 0.3±0.48,4.2±0.95 vs 0.3±0.48,均P<0.01);ANP组大鼠[99mTc]-DTPA排泄率术后12,24和36h较对照组均明显增加(34.70%±4.03% vs 4.62%±1.17%,54.63%±6.94% vs 6.14%±1.42%,66.83%±7.56% vs 7.48%±0.92%,均P<0.01);与对照组相比,ANP组大鼠小肠组织中regⅠ的mRNA水平过度表达,regⅠ的表达水平分别与肠黏膜病理学评分和肠黏膜通透性指数正相关(r=0.6728,0.7092,均P<0.01).结论:ANP时大鼠小肠组织中regⅠ mRNA的表达水平明显上调且与ANP所致肠黏膜损害的严重程度相关.展开更多
目的:联合应用cDNA微阵列和组织微阵列技术并采用计算机辅助处理技术研究正常胰腺组织、MAP、SAP之间基因表达谱,筛选出MAP与正常粘膜以及MAP与SAP之间的差异表达基因。方法:分别抽提正常胰腺组织、MAP和SAP组织的总RNA并纯化mRNA;将各m...目的:联合应用cDNA微阵列和组织微阵列技术并采用计算机辅助处理技术研究正常胰腺组织、MAP、SAP之间基因表达谱,筛选出MAP与正常粘膜以及MAP与SAP之间的差异表达基因。方法:分别抽提正常胰腺组织、MAP和SAP组织的总RNA并纯化mRNA;将各mR-NA逆转录合成以Cy5和Cy3标记的cDNA一链做探针,分别混合后在3张含有4096条双点人类全长基因的芯片上进行杂交。用ScanArray 4000扫描芯片荧光信号图像,用GenePix Pro 3.0软件对扫描图像进行数字化处理和分析。结果3次杂交出现一致性显著异常,表达差异在2倍以上的基因有141条,其中表达上调的74条,表达下调的67条。结论通过基因表达谱差异的比较,提示MAP和SAP在基因水平存在差异,差异2倍以上的141个基因可能与AP的发生和发展以及相关早期炎症的启动和演化有关。展开更多
文摘Objectives.To study the expression of TNF α mRNA and the effect of somatostatin on the expression of TNF α mRNA in multiorgans of rats with acute hemorrhagic necrotic pancreatitis(AHNP). Methods.AHNP in the rat was induced by retrograde injection of 5% sodium taurocholate into the bile-pancreatic duct. Somatostatin octapeptide (SS-OP) (2μg/kg)was injected into the femoral vein imme- diately in rats of the treatment group after inductive AHNP. Rats of the sham operative group received in- jection of saline. Sixty animals of the AHNP and sham operative groups at the designated time(0. 2h, 0. 5 h, 2h, 4h, 8h, after the operation,six animals at each time point)and tweleve animals of treatment group at 4h after the operation were sacrificed for samples of pancreas, liver and lung. The expressions of TNF α mRNA within the pancreas, liver and lung were established by RT－PCR. Results. TNF α mRNA became detectable in the pancreas as early as 0. 2h after inductive AHNP, while it was undetectable in other organs until 0. 5h. Expression of TNF α mRNA in each tissue increased continuously and reached a peak at 4h,demonstrating a significant difference compared with that at 0. 5h and 8h. Expressions of TNF α mRNA from pancreas, liver and lung were decreased 50-80% in the treat- ment group, the pancreatic necrosis was also attenuated dramatically. Conclusion. TNF α mRNA was detectable in pancreas,liver and lung tissues at the early stage of AH- NP.SS-OP can significantly inhibit the expression of TNF α mRNA and attenuate the pancreatic necrosis. We feel that this may be an important mechanism of SS-OP in the treatment of AHNP.
文摘Acute necrotising pancreatitis is characterized by inflammatory and necrotic events, which followthe initial intra-acinar injury involving enzyme activation, and disruption of the acinar cytoskeleton. At present, apoptosis has become a hot topic in many kinds of disease. Bax and Bcl-2 are the important control gene during cell apoptosis. 2 Previous study has shown that interleukin 10 （IL-10） is a kind of important antiinflammatory cytokine and plays a role of selfdefense mechanism,
文摘目的:研究regⅠ在急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)大鼠肠组织中的表达,探讨该基因的表达与ANP肠黏膜损害的关系.方法:Sprague-Dawley大鼠随机分为假手术对照组(n=40)和ANP组(n=80).对照组开腹后只翻动胰腺,ANP组大鼠胰胆管恒速逆行注射30g/L牛磺胆酸钠,制成ANP大鼠模型.观察胰腺和小肠的病理改变及小肠黏膜通透性的改变,应用逆转录聚合酶链反应(RT-PCR)检测胰腺、小肠组织中regⅠ mRNA的表达水平,并研究所观察指标与regⅠ基因表达的相关性.结果:ANP组大鼠术后12,24和36h肠黏膜组织病理学评分明显高于对照组(1.8±0.89 vs 0.2±0.42,3.3±1.17 vs 0.3±0.48,4.2±0.95 vs 0.3±0.48,均P<0.01);ANP组大鼠[99mTc]-DTPA排泄率术后12,24和36h较对照组均明显增加(34.70%±4.03% vs 4.62%±1.17%,54.63%±6.94% vs 6.14%±1.42%,66.83%±7.56% vs 7.48%±0.92%,均P<0.01);与对照组相比,ANP组大鼠小肠组织中regⅠ的mRNA水平过度表达,regⅠ的表达水平分别与肠黏膜病理学评分和肠黏膜通透性指数正相关(r=0.6728,0.7092,均P<0.01).结论:ANP时大鼠小肠组织中regⅠ mRNA的表达水平明显上调且与ANP所致肠黏膜损害的严重程度相关.
文摘目的:联合应用cDNA微阵列和组织微阵列技术并采用计算机辅助处理技术研究正常胰腺组织、MAP、SAP之间基因表达谱,筛选出MAP与正常粘膜以及MAP与SAP之间的差异表达基因。方法:分别抽提正常胰腺组织、MAP和SAP组织的总RNA并纯化mRNA;将各mR-NA逆转录合成以Cy5和Cy3标记的cDNA一链做探针,分别混合后在3张含有4096条双点人类全长基因的芯片上进行杂交。用ScanArray 4000扫描芯片荧光信号图像,用GenePix Pro 3.0软件对扫描图像进行数字化处理和分析。结果3次杂交出现一致性显著异常,表达差异在2倍以上的基因有141条,其中表达上调的74条,表达下调的67条。结论通过基因表达谱差异的比较,提示MAP和SAP在基因水平存在差异,差异2倍以上的141个基因可能与AP的发生和发展以及相关早期炎症的启动和演化有关。
