Background Gossypium hirsutum undergoes rapid clonal propagation to regenerate a mature plant through tissue culture.However,the correlation between cotton leaf regeneration,callus induction,and regeneration ability w...Background Gossypium hirsutum undergoes rapid clonal propagation to regenerate a mature plant through tissue culture.However,the correlation between cotton leaf regeneration,callus induction,and regeneration ability was still obscure.In this research,cotton leaf regeneration level for 21 accessions in the field(new leaves)was observed after the first harvest,and a comparison between field regeneration level and callus induction with its regeneration capacity(new shoots and roots)for the same 21 accessions was carried out.Agronomic traits,including plant height,leaf area,fresh leaf weight,dry leaf weight,the number of flowers and bolls,and biochemical(proline content)and physiological(chlorophyll and carotenoid content)traits during the flowering stage of 21 upland cotton accessions,were investigated.Result A significant correlation between physiological parameters and callus induction was discovered.Callus induction and regeneration capacity of roots and shoots for hypocotyl,cotyledons,and shoot tip tissues were used to validate field leaf regeneration level after the first harvest.CCRI 24 showed significant leaf regeneration in the field and callus induction capacity through callus induction and regeneration.Conclusion We found a substantial relationship between field regeneration capability and callus induction with its regeneration capacity for the hypocotyl,cotyledons,and shoot tip.The results showed that ZS061,Lumian 378,Jimian 863,and ZS065 have the highest moisture retention capacity,while CCRI 24,Liaoyang Duomaomian,and Beizhe Gongshemian have the lowest moisture retention capacity.CCRI 24 has the highest leaf regeneration capacity in the field,while Beizhe Gongshemian has the lowest leaf regeneration capacity.All our result provides a clue for checking the regeneration capacity through leaf regeneration level in the field.展开更多
Prunus africana is a species of great economic, medicinal and ecological importance. Due to its multiple uses, unsustainable exploitation methods and low regeneration capacity in tropical rainforests, this species is ...Prunus africana is a species of great economic, medicinal and ecological importance. Due to its multiple uses, unsustainable exploitation methods and low regeneration capacity in tropical rainforests, this species is threatened with extinction. Present and exploited in the eastern part of DR Congo, knowledge of the dynamics of post-bark regeneration of Prunus africana remains fragmentary and poorly known. In North Kivu province, this species thrives in both afromontane forest and lowland tropical rainforest habitats. In order to contribute to the rational and sustainable exploitation of Prunus africana in this province, this paper was carried out with the objective of contributing to the knowledge of the dynamics of the regeneration of post-harvest bark of Prunus africana in two exploitation sites (low and high altitude). To achieve this objective, the inventory was conducted on 16 plots of 25 hectares each, with 8 plots per site. Dendrometric parameters (diameter at breast height (DBH), total tree height) and tree growth and regeneration parameters, i.e., stem bark thickness of the unharvested and harvested portions of the trees (bark reconstitution) were measured. A total of 716 barked stems of Prunus africana in 2016 in 25 hectares constituted the study sample. Results show that sites do not influence diameter at breast height of P. africana trees (p > 0.05) or total tree height. The bark diameter of harvested trees and the bark diameter of unharvested trees varied significantly by site (p 0.05). In contrast, the annual growth rate of bark differed with altitude;the highest rate was observed in trees growing at high altitude (2.97 ± 0.9 mm/yr) compared to 2.23 ± 0.74 mm/yr at low altitude. In view of these results, this study indicates that a half-rotation of 7 years could allow an effective reconstitution of the bark of Prunus africana at the second passage on the remaining side of the same stem.展开更多
基金supported by Ministry of Agriculture and Rural Affairs(19221957).
文摘Background Gossypium hirsutum undergoes rapid clonal propagation to regenerate a mature plant through tissue culture.However,the correlation between cotton leaf regeneration,callus induction,and regeneration ability was still obscure.In this research,cotton leaf regeneration level for 21 accessions in the field(new leaves)was observed after the first harvest,and a comparison between field regeneration level and callus induction with its regeneration capacity(new shoots and roots)for the same 21 accessions was carried out.Agronomic traits,including plant height,leaf area,fresh leaf weight,dry leaf weight,the number of flowers and bolls,and biochemical(proline content)and physiological(chlorophyll and carotenoid content)traits during the flowering stage of 21 upland cotton accessions,were investigated.Result A significant correlation between physiological parameters and callus induction was discovered.Callus induction and regeneration capacity of roots and shoots for hypocotyl,cotyledons,and shoot tip tissues were used to validate field leaf regeneration level after the first harvest.CCRI 24 showed significant leaf regeneration in the field and callus induction capacity through callus induction and regeneration.Conclusion We found a substantial relationship between field regeneration capability and callus induction with its regeneration capacity for the hypocotyl,cotyledons,and shoot tip.The results showed that ZS061,Lumian 378,Jimian 863,and ZS065 have the highest moisture retention capacity,while CCRI 24,Liaoyang Duomaomian,and Beizhe Gongshemian have the lowest moisture retention capacity.CCRI 24 has the highest leaf regeneration capacity in the field,while Beizhe Gongshemian has the lowest leaf regeneration capacity.All our result provides a clue for checking the regeneration capacity through leaf regeneration level in the field.
文摘Prunus africana is a species of great economic, medicinal and ecological importance. Due to its multiple uses, unsustainable exploitation methods and low regeneration capacity in tropical rainforests, this species is threatened with extinction. Present and exploited in the eastern part of DR Congo, knowledge of the dynamics of post-bark regeneration of Prunus africana remains fragmentary and poorly known. In North Kivu province, this species thrives in both afromontane forest and lowland tropical rainforest habitats. In order to contribute to the rational and sustainable exploitation of Prunus africana in this province, this paper was carried out with the objective of contributing to the knowledge of the dynamics of the regeneration of post-harvest bark of Prunus africana in two exploitation sites (low and high altitude). To achieve this objective, the inventory was conducted on 16 plots of 25 hectares each, with 8 plots per site. Dendrometric parameters (diameter at breast height (DBH), total tree height) and tree growth and regeneration parameters, i.e., stem bark thickness of the unharvested and harvested portions of the trees (bark reconstitution) were measured. A total of 716 barked stems of Prunus africana in 2016 in 25 hectares constituted the study sample. Results show that sites do not influence diameter at breast height of P. africana trees (p > 0.05) or total tree height. The bark diameter of harvested trees and the bark diameter of unharvested trees varied significantly by site (p 0.05). In contrast, the annual growth rate of bark differed with altitude;the highest rate was observed in trees growing at high altitude (2.97 ± 0.9 mm/yr) compared to 2.23 ± 0.74 mm/yr at low altitude. In view of these results, this study indicates that a half-rotation of 7 years could allow an effective reconstitution of the bark of Prunus africana at the second passage on the remaining side of the same stem.
