期刊文献+
共找到15篇文章
< 1 >
每页显示 20 50 100
Uniqueness of the Gossypium mustelinum Genome Revealed by GISH and 45S rDNA FISH
1
作者 STELLY David 《棉花学报》 CSCD 北大核心 2008年第S1期110-,共1页
Gossypium mustelinum [(AD)4] is one of five tetraploid species in Gossypium.Three pairs of nucleolar organizer regions(NOR) in(AD)4 were detected by FISH with 45S rDNA as a probe,they also were observed with genomic D... Gossypium mustelinum [(AD)4] is one of five tetraploid species in Gossypium.Three pairs of nucleolar organizer regions(NOR) in(AD)4 were detected by FISH with 45S rDNA as a probe,they also were observed with genomic DNA(gDNA) from Gossypium D genome species as probes.Of 展开更多
关键词 Gossypium mustelinum cotton in situ hybridization nucleolar organizer region chromosomes GENOME
下载PDF
CORRELATIONSHIP BETWEEN CELLULAR DNA AND AgNOR PROTEIN CONTENT IN THE DEVELOPING COURSE OF COLORECTAL ADENOCARCINOMA
2
作者 谢尧 屈汉廷 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1995年第4期280-283,共4页
Cellular NDA and AgNOR Protein contents were evaluated byautomatic image analysis in tissue sections stained hy combined Feulgen-AgNOR staining method in 9 normal colonic mucosae, 45 colorectal adenomas and 27 adenoca... Cellular NDA and AgNOR Protein contents were evaluated byautomatic image analysis in tissue sections stained hy combined Feulgen-AgNOR staining method in 9 normal colonic mucosae, 45 colorectal adenomas and 27 adenocarcinomas. The results indicated that during the course that the normal colonical mucosa developed to colorectal adenocarcinoma via adenoma the DNA and AgNOR protein contents increased gradually and there were very significant correlationships between the DNA and the AgNOR protein contents of adenoma group and adenocarcinoma group. However, there were considerahle overlaping in the DNA or AgNOR Protein content and considerahle overlaping cases between adenoma and normal colonic mucosa groups and between adenoma and adenocarcinoma groups. But the overlaping scope in NDA and AgNOR Protein content and the number of overlaping cases were reduced significantly by assessing the correlationship between the DNA and AgNOR protein content. Therefore, it is much more reliable to distinguish colorectal adenomas from adnocarcinomas by using the correlationship between the cellular DNA and the AgNOR Protein contents in the same specimens. 展开更多
关键词 Colorectal Tumor Adenoma Adenocarcinoma Deoxyribonucleic acid Tumor AgNOR protein Nucleolar Organizer Regions
下载PDF
NUCLEOLAR ORGANIZER REGIONS IN CUTANEOUS T CELL LYMPHOMAS
3
作者 许良中 陈红莉 +1 位作者 邱丙森 陶玲娣 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1990年第3期61-64,共4页
The present work studied the application of AgNOR count to differential diagnosis between cutaneous T cell lymphoma (CTCL) and cutaneous pseudolymphoma (CPL). Paraffin sections from 50 mycosis fungoides (22 MFI-Premyc... The present work studied the application of AgNOR count to differential diagnosis between cutaneous T cell lymphoma (CTCL) and cutaneous pseudolymphoma (CPL). Paraffin sections from 50 mycosis fungoides (22 MFI-Premycotic stage, 24 MF Ⅰ infiltrative stage and 4 MF Ⅲ - tumor stage), 2 nonepidermotropic cutaneous T cell lymphoma (NECTCL) and 9 CPL were investigated. In each case, 200 cells randomly selected were examined using a × 100 oil immersion lens. The mean number, standard deviation and standard error of the mean of AgNOR counts were as follows: MFⅠ 1.17±0.09, SEM = 0.01; MⅡ 1.17±0.01, SEM = 0.01; MF Ⅲ. 3.55±0.87, SEM = 0.43; NECTCL 4.5±0.28, SEM -0.199; CPL 1.17±0.1, SEM ± 0.03. The results revealed a highly significant difference between CTCL (MFⅢ+NECTCL) and CPL (t = 4.75, P<0.001), tumor stage (MF Ⅲ) and pretumor stage (MFI, MF Ⅱ) of mycosis fungoides (t = 4.75, P<0.001). Thus. AgNOR count is valuable in differential diagnosis. 展开更多
关键词 AGNORS MFI NUCLEOLAR ORGANIZER REGIONS IN CUTANEOUS T CELL LYMPHOMAS
下载PDF
Argyrophilic nucleolar organizer region in MIB-1 positive cells in non-small cell lung cancer: clinicopathological significance and survival
4
作者 Dmitriy Sergeevich Kobyakov Ashot Merudzhanovich Avdalyan +2 位作者 Aleksandr Fedorovich Lazarev Elena Leonidovna Lushnikova Lev Moiseevich Nepomnyashchikh 《Cancer Biology & Medicine》 SCIE CAS CSCD 2014年第4期264-269,共6页
Objective: To evaluate the relation between argyrophilic nucleolar organizer region (AgNOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer (NSCLC). Methods: A t... Objective: To evaluate the relation between argyrophilic nucleolar organizer region (AgNOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer (NSCLC). Methods: A total of 207 surgical specimens diagnosed as NSCLC were included in this study. Double-staining procedures were performed using antigen Ki-67 (clone MIB-1) and silver nitrate by immunohistochemical and AgNOR-staining methods. Results: The AgNOR area in MIB-l-positive cells of NSCLC is related to clinicopathological parameters under the TNM (tumor, node, and metastasis) system. The survival of patients with small AgNOR area in MIB-1-positive cells is better than that of patients with large AgNOR area. Molecular, biological (AgNOR area in MIB-l-positive cells), and clinicopathological (greatest tumor dimension, metastases to regional lymph nodes, histology, and differentiation) parameters are independent prognostic factors of NSCLC.Conclusion: The AgNOR area in MIB- 1-positive cells is related to clinicopathological parameters and survival in NSCLC. 展开更多
关键词 Argyrophilic nucleolar organizer region (AgNOR) MIB-1 SURVIVAL non-small cell lung cancer (NSCLC)
下载PDF
Interferon plus ribavirin and interferon alone in preventing hepatocellular carcinoma: A prospective study on patients with HCV related cirrhosis
5
作者 Francesco Azzaroli Esterita Accogli +12 位作者 Giovanni Nigro Davide Trerè Silvia Giovanelli Anna Miracolo Francesca Lodato Marco Montagnani Mariarosa Tamé Antonio Cloecchia Constance Mwangemi Davide Festi Enrico Roda Massimo Derenzini Giuseppe Mazzella 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第21期3099-3102,共4页
AIM:To determine the role of interferon(IFN)with or withoutribavirin in preventing or delaying hepatocellular carcinoma(HCC)development in patients with hepatitis C virus(HCV)related cirrhosis.Data on the preventive e... AIM:To determine the role of interferon(IFN)with or withoutribavirin in preventing or delaying hepatocellular carcinoma(HCC)development in patients with hepatitis C virus(HCV)related cirrhosis.Data on the preventive effect of IFN plusribavirin treatment are lacking.METHODS:A total of 101 patients(62 males and 39 females,mean age 55.1±1.4 years)with histologically proven HCVrelated liver cirrhosis plus compatible biochemistry andultrasonography were enrolled in the study.Biochemistryand ultrasonography were performed every 6 mo.Ultrasoundguided liver biopsy was performed on all detected focallesions.Follow-up lasted for 5 years.Cellular proliferation,evaluated by measuring Ag-NOR proteins in hepatocytesnuclei,was expressed as AgNOR-Proliferative index(AgNOR-PI)(cut-off=2.5).Forty-one patients(27 males,14 females)were only followed up after the end of anyearly treatment with IFN-alpha2b(old treatment controlgroup=OTCG).Sixty naive patients were stratified accordingto sex and AgNOR-PI and then randomized in two groups:30 were treated with IFN-alpha2b+ribavirin(treatmentgroup=TG),the remaining were not treated(control group=CG).Nonresponders(NR)or relapsers in the TG receivedfurther IFN/ribavirin treatments after a 6 mo of withdrawal.RESULTS:AgNOR-PI was significantly lowered by IFN(P<0.001).HCC incidence was higher in patients withAgNOR-PI>2.5(26% vs3%,P<0.01).Two NR in the OTCG,none in the TG and 9 patients in the CG developed HCCduring follow-up.The Kaplan-Mayer survival curves showedstatistically significant differences both between OTCG andCG(P<0.004)and between TG and CG(P<0.003).CONCLUSION:IFN/ribavirin treatment associated with re-treatment courses of NR seems to produce the best resultsin terms of HCC prevention.AgNOR-PI is a useful markerof possible HCC development. 展开更多
关键词 Antineoplastic Agents DOSAGE Antiviral Agents Carcinoma Hepatocellular control Drug Therapy Combination Female Hepatitis C Chronic Humans INTERFERONS Liver Cirrhosis Liver Neoplasms Male Middle Aged Nucleolus Organizer Region Prospective Studies RIBAVIRIN Silver Staining
下载PDF
STUDIES OF NUCLEOLAR ORGANIZER REGIONS AND MUCIN HISTOCHEMISTRY IN ULCERATIVE COLITIS
6
作者 虞积耀 杜毅 +4 位作者 夏敏良 胡端民 王鲁平 邢惠清 田玉旺 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 1992年第2期46-50,共5页
The argyrophil method for nucleolar organizer regions (AgNOR) known as the AgNor technique and mucin histochemical stain were applied to Investigate the dysplasia and cancaration in ulcerative colitis Including 58 cas... The argyrophil method for nucleolar organizer regions (AgNOR) known as the AgNor technique and mucin histochemical stain were applied to Investigate the dysplasia and cancaration in ulcerative colitis Including 58 cases of biopsy specimens and three cases operative specimens. The numbers of AgNOR have been gradully increased with the grades of dasplasls. Similarly the percentage of sialomucin having vicinal diols on side chain of sialic acid also gradully Increased with the grades of dysplasia. The AgNOR reflects rDNA transcriptional activity responsible for degrees of differentiation of cell. Epithelial cells secreting a heterogeneous mucin, could be taken as a signal of abnormal cellular differentiation. AgNOR and mucln chages might be assumed as a criteria In representing malignant transformation. 展开更多
关键词 AGNOR STUDIES OF NUCLEOLAR ORGANIZER REGIONS AND MUCIN HISTOCHEMISTRY IN ULCERATIVE COLITIS
下载PDF
ORGANIZING MACRO ECONOMIC REGIONS AND ENHANCING INTER-PROVINCIAL COORDINATION AND PLANNING IN CHINA
7
作者 Hu Xuwei(Institute of Geography, CAS, Beijing 100101People’s Republic of China) 《Journal of Geographical Sciences》 SCIE CSCD 1995年第1期1-9,共9页
This paper elaborates upon the purpose of macro economicroponalization and inter-provincial planning and coordination under thesocialist market system. It descnbes the characteristics and orgarnzationalforms of macro ... This paper elaborates upon the purpose of macro economicroponalization and inter-provincial planning and coordination under thesocialist market system. It descnbes the characteristics and orgarnzationalforms of macro economic regions and the principle of macro economic regionalization. The six macro economic regions-Northeast China, NorthChina, Northwest China, Middle-East China, Southwest China and South China-are divided in the paper. At the end, the paper describes somemajor programs, which requires cooperation among various involvedprovinces. 展开更多
关键词 macro economic region organization of provinces and autonomous regions planning and coordination
下载PDF
MEMBER PRIVILEGES——Each of the Shanghai Cooperation Organization members finds its own national interests served within the regional organization
8
作者 SIDOROV OLEG 《Beijing Review》 2006年第24期20-21,共2页
Facing common security threats, leaders of six countries-Russia, China, Kazakhstan, Kyrgyzstan, Tajikistan and Uzbekistan-established the Shanghai Cooperation Organization in June 2001 to maintain regional stability a... Facing common security threats, leaders of six countries-Russia, China, Kazakhstan, Kyrgyzstan, Tajikistan and Uzbekistan-established the Shanghai Cooperation Organization in June 2001 to maintain regional stability and security, fight terrorism and extremism, prevent conflicts and enhance economic cooperation. The SCO's primary goals are economic cooperation and fighting terrorism. 展开更多
关键词 SCO Ky ORGANIZATION MEMBER PRIVILEGES Each of the Shanghai Cooperation Organization members finds its own national interests served within the regional organization
原文传递
Uniqueness of the Gossypium mustelinum Genome Revealed by GISH and 45S rDNA FISH 被引量:4
9
作者 Qiong Wu Fang Liu +6 位作者 Shaohui Li Guoli Song Chunying Wang Xiangdi Zhang Yuhong Wang David Stelly Kunbo Wang 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2013年第7期654-662,共9页
Gossypium mustelinum ((AD)4) is one of five disomic species in Gossypium. Three 45S ribosomal DNA (rDNA) loci were detected in (AD)4 with 45S rDNA as probe, and three pairs of brighter signals were detected wi... Gossypium mustelinum ((AD)4) is one of five disomic species in Gossypium. Three 45S ribosomal DNA (rDNA) loci were detected in (AD)4 with 45S rDNA as probe, and three pairs of brighter signals were detected with genomic DNA (gDNA) of Gossypium D genome species as probes. The size and the location of these brighter signals were the same as those detected with 45S rDNA as probe, and were named GISH-NOR. One of them was super-major, which accounted for the fact that about one-half of its chromosome at metaphase was located at chromosome 3, and other two were minor and located at chromosomes 5 and 9, respectively. All GISH-NORs were located in A sub-genome chromosomes, separate from the other four allopolyploid cotton species. GISH-NOR were detected with D genome species as probe, but not A. The greatly abnormal sizes and sites of (AD)4 NORs or GISH-NORs indicate a possible mechanism for 45S rDNA diversification following (AD)4 speciation. Comparisons of GISH intensities and GISH-NOR production with gDNA probes between A and D genomes show that the better relationship of (AD)4 is with A genome. The shortest two chromosomes of A sub-genome of G. mustelinum were shorter than the longest chromosome of D sub-genome chromosomes. Therefore, the longest 13 chromosomes of tetraploid cotton being classified as A sub-genome, while the shorter 13 chromosomes being classified as D sub-genome in traditional cytogenetic and karyotype analyses may not be entirely correct. 展开更多
关键词 Chromosomes cotton Gossypium mustelinum in situ hybridization nucleolar organizer region genome.
原文传递
Genome-Wide Analysis of DNA Methylation in Soybean 被引量:7
10
作者 Qing-Xin Song Xiang Lu +6 位作者 Qing-Tian Li Hui Chen Xing-Yu Hu Biao Ma Wan-Ke Zhang Shou-Yi Chen Jin-Song Zhang 《Molecular Plant》 SCIE CAS CSCD 2013年第6期1961-1974,共14页
Cytosine methylation is an important mechanism for dynamical regulation of gene expression and trans- posable element (TE) mobility during plant developmental processes. Here, we identified the transcription start s... Cytosine methylation is an important mechanism for dynamical regulation of gene expression and trans- posable element (TE) mobility during plant developmental processes. Here, we identified the transcription start sites of genes using high-throughput sequencing and then analyzed the DNA methylation status in soybean roots, stems, leaves, and cotyledons of developing seeds at single-base resolution. Profiling of DNA methylation in different organs revealed 2162 differentially methylated regions among organs, and a portion of hypomethylated regions were correlated with high expression of neighboring genes. Because of the different distribution of class I TEs (retrotransposons) and class II TEs (DNA transposons), the promoters of the lowest-expressed genes showed higher levels of CG and CHG methyla- tion but a lower level of CHH methylation. We further found that the CHH methylation level of class II TEs was higher than class I TEs, possibly due to the presence of more smRNAs in class II TEs. In cotyledons of developing seeds, smRNA abundance was roughly positively correlated with hypermethylated regions but negatively related to hypomethylated regions. These studies provide significant insights into the complicated interplays among DNA methylation, smRNA abundance, TE distribution, and gene expression in soybean. 展开更多
关键词 SOYBEAN DNA methylation differentially methylated regions organs.
原文传递
Synergistic impacts of anthropogenic and biogenic emissions on summer surface O_3 in East Asia 被引量:4
11
作者 Yu Qu Junling An Jian Li 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2013年第3期520-530,共11页
A factor separation technique and an improved regional air quality model (RAQM) were applied to calculate synergistic contributions of anthropogenic volatile organic compounds (AVOCs),biogenic volatile organic com... A factor separation technique and an improved regional air quality model (RAQM) were applied to calculate synergistic contributions of anthropogenic volatile organic compounds (AVOCs),biogenic volatile organic compounds (BVOCs) and nitrogen oxides (NOx) to daily maximum surface O3(O3DM) concentrations in East Asia in summer (June to August 2000).The summer averaged synergistic impacts of AVOCs and NOx are dominant in most areas of North China,with a maximum of 60 ppbv,while those of BVOCs and NOx are notable only in some limited areas with high BVOC emissions in South China,with a maximum of 25 ppbv.This result implies that BVOCs contribute much less to summer averaged O3DM concentrations than AVOCs in most areas of East Asia at a coarse spatial resolution (1×1) although global emissions of BVOCs are much greater than those of AVOCs.Daily maximum total contributions of BVOCs can approach 20 ppbv in North China,but they can reach 40 ppbv in South China,approaching or exceeding those in some developed countries in Europe and North America.BVOC emissions in such special areas should be considered when O3 control measures are taken.Synergistic contributions among AVOCs,BVOCs and NOx significantly enhance O3 concentrations in the Beijing-Tianjin-Tangshan region and decrease them in some areas in South China.Thus,the total contributions of BVOCs to O3DM vary significantly from day to day and from location to location.This result suggests that O3 control measures obtained from episodic studies could be limited for long-term applications. 展开更多
关键词 regional air quality model volatile organic compounds O3 factor separation technique synergistic contribution
原文传递
Nucleolar Organizer Regions in Oreochromis nilotica Chromosomes
12
作者 傅莉娟 《Tsinghua Science and Technology》 SCIE EI CAS 1997年第1期17-21,共5页
The nucleolar organizer regions (NORs) in the mitotic chromosomes of tilapia Oreochromis niloticus were analyzed using silver nitrate staining Ag detected NORs(Ag NORs) in the chromosomes of the tilapia exhibited he... The nucleolar organizer regions (NORs) in the mitotic chromosomes of tilapia Oreochromis niloticus were analyzed using silver nitrate staining Ag detected NORs(Ag NORs) in the chromosomes of the tilapia exhibited heteromorphism. Individual Ag NORs sizes varied on different chromosomes, and the number of Ag NOR per cell varied from three to six. Among the examined 100 cells, 71% of the gnomes carried four Ag NORs, 12% carried 3,11% carried 5, and 6% carried 6. The variance occurred not only among different cells but also among different cells from the same source. The satellite association between homologous chromosomes in No 7 was also observed in 27% of the metaphase chromosome preparations. The heteromorphic properties of Ag NORs were discussed. 展开更多
关键词 fish metaphase chromosome nucleolus organizer region heteromorphism satellite association
原文传递
Objectifying Research on Special Region Corresponding to Five Internal Organs in Colour Inspections of Chinese Medicine
13
作者 郑冬梅 戴振东 王鸿谟 《Chinese Journal of Integrative Medicine》 SCIE CAS 2014年第10期737-742,共6页
Objective:To study the differences in a special region(SR) corresponding to five internal organs between the young and the elderly and to verify the effectiveness of a developed system for objectifying research in ... Objective:To study the differences in a special region(SR) corresponding to five internal organs between the young and the elderly and to verify the effectiveness of a developed system for objectifying research in colour inspections of Chinese medicine(CICM).Methods:An image acquisition device imitating daylight to meet the requirement of CICM was designed.Image processing software based on CICM theory was developed.A total of 83 normal people were sampled and assigned to two groups.The first group(Group I)included 30 people with an average age of 24 ±2,and the second group(Group Ⅱ) included 53 people with an average age of 74 ±4.After image processing,the chromaticity of 10 SRs corresponding to five internal organs was calculated.R,G,and B represent the chromaticity of color red,green,and blue,L indicates color brightness,whereas a and b denote red and yellow respectively.The difference between the young and the elderly was analyzed.Results:(1) The chromaticity of each SR corresponding to five organs was obtained.The average R,G,B,L,a,and b of the sample were 194.4,147.6,119.3,65.2,15.5,and 22.3,respectively.(2) The R,G,B,and L of the young were greater than those of the elderly,but the a and b of the young were less than those of the elderly.(3) A statistically significant difference in each SR was found between the young and the elderly(P0.05).The total differences for the Lung(Fei),Heart(Xin),Liver(Gan),Spleen(Pi),and Kidney(Shen) were 50%(3/6),66.7%(4/6),66.7%(4/6),83.3%(5/6),and 91.7%(33/36),respectively.However,the Kidney SR had the greatest statistical discrepancy.Conclusion:The old Chinese saying that human Kidney deficiency worsens with age was verified by the results.The objectifying system used to examine CICM was effective.Thus,this system may be used as a basis for further research. 展开更多
关键词 colour inspection of Chinese medicine special region corresponding to five internal organs chromaticity Chinese medicine objectifying research
原文传递
Euro Experience and the Possibility of Financial&Monetary Integration in Arab Gulf Countries
14
作者 Li Yang 《Asian Journal of Middle Eastern and Islamic Studies》 2009年第2期48-58,I0010,共12页
Based on the theories of Optimum Currency Areas and Impossible Triangle,this article considers the practice analysis of the Euro and European Central Bank,and proposes the possibility of monetary integration in Arab G... Based on the theories of Optimum Currency Areas and Impossible Triangle,this article considers the practice analysis of the Euro and European Central Bank,and proposes the possibility of monetary integration in Arab Gulf countries.The author argues that at this stage,member states must collaborate closely,and coordinate fully among various conflicts.Only by doing this will they be able to create necessary conditions for upgrading the level of financial and monetary integration in Arabic Gulf countries. 展开更多
关键词 EURO European Central Bank(ECB) Financial&Monetary Integration Gulf Cooperation Council Gulf Economy Impossible Triangle Theory Optimum Currency Areas regional Organization
原文传递
From Southeast Asia to the Gulf:Analysis of the Prospect of Indian Ocean Integration
15
作者 YU Zhen BAI Xinyi 《Asian Journal of Middle Eastern and Islamic Studies》 2017年第3期90-106,共17页
The Indian Ocean is not only a geographic unit,but also a sub-system of the international system constructed within the interactions among the coastal countries.This article chooses the regional organizations as a var... The Indian Ocean is not only a geographic unit,but also a sub-system of the international system constructed within the interactions among the coastal countries.This article chooses the regional organizations as a variable to analyze the interactions among Indian Ocean coastal countries.With the empirical studies on the regional cooperation organizations,this article discloses the dynamics behind the regional integration between costal countries along the Indian Ocean Region.Six most representative regional cooperation organizations have been analyzed by this article,which are respectively the Cooperation Council for the Arab States of the Gulf(GCC),South Asian Association for Regional Cooperation(SAARC),Association of Southeast Asian Nations(ASEAN),Southern African Development Community(SADC),and Common Market for Eastern and Southern Africa(COMESA),as well as Indian Ocean Rim Association(IORA).They could make purposive and rational decisions within the institutional constraints.Based on case studies and comparative analysis,this article suggests that there are four significantly different cooperation models among the coastal countries,and suggests not simply estimate the likely regional powers or organizations that would lead the construction of the regional cooperation,but the great importance of building the linkage among various regional cooperation institutions. 展开更多
关键词 regional Cooperation Organization Indian Ocean Region regional Integration the Gulf ASEAN
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部