Prokaryotic Expression and Immunogenicity of Dominant Epitope Region of Goose Parvovirus Structural Protein VP3

[Objective] The paper was to develop a subunit vaccine candidate for prevention and control of goose parvovirus infection. [Method]Based on the prokaryotic expression system, the antigenic epitopes and locations of the structural protein VP3 were predicted by software analysis,and the region displaying a large portion of antigenic epitopes was amplified by PCR. The target VP3 DNA fragment was inserted into pET-30 a-VP3 vector, was transformed into Escherichia coli BL21 competent cells for protein expression and animal test. The SPF chickens were immunized with the recombinant protein and the antisera were collected for neutralization test by using a goose embryo fibroblast. [Result] The recombinant plasmid was constructed, and the target region of VP3 protein was expressed efficiently in a soluble form. The neutralizing titers of antisera could reach up to-2.608. [Conclusion] The target region displaying a large portion of antigenic epitopes of the structural protein VP3 could be expressed efficiently in soluble form, and the expressed protein could induce neutralizing antibodies in SFP chicken.

IDENTIFYING OPTIMUM GLAZING PROPERTY FOR CONSERVING ENERGY IN HOT SEMI-ARID CLIMATE REGIONS

Globally,the building sector is responsible for 40%of energy use and 30%of GHG emissions.The greatest portion of the energy is used during the operational phase(use stage)of buildings.The building envelope,especially the glazed components,plays an important role in determining the energy requirement of buildings.These glazed parts of the building envelope exposed to direct solar radiation are most vulnerable to heat loss and gain.Heat loss and gain through the glazing material depend on glazing properties(U-value,SHGC,VT)and building energy use changes according to the properties of the glazing system.A variety of glazing types has been developed over recent decades that use the properties of the glass as a means of responding to environmental conditions.This study is carried out to identify the optimum glazing property for conserving energy in cooling dominant regions using an early design energy modeling tool.It was found that a low SHGC is the most important glazing property for reducing cooling energy consumption.SHGC of less than 0.3 is found useful.This study would help building industry professionals evaluate the best glazing property while selecting the glazing type.

FLUSHING TIME OF THE YANGTZE ESTUARY BY DISCHARGE: A MODEL STUDY

Flushing time of the Yangtze estuary by discharge is one of the importantfactors responsible for the transport of pollutants from various sources located along the Yangtzeestuary: Therefore, an objective of the present stud-y, which analysis flushing time in the case ofdifferent discharge is very helpful to evaluate the water environmental of the Yangtze estuary.Using a dissolved conservative material as a tracer in the water, a three-dimensionadvection-diffusion water exchange numerical model was used to study the flushing time by dischargeand the discharge dominated region of the Yangtze estuary. The initial tracer concentration is setto 0.0 in the numerical domain of the Yangtze estuary, and the concentration value is set to 1. 0 onthe inflow boundary. The tracer flux normal to the solid boundary is set to 0. 0. The flushing timeand the out limit of discharge dominated region can be calculated in terms of the tracerconcentration. Estuarine, Coastal and Ocean Model (ECOM) is used as the hydro-dynamic model. Theresult shows that the flushing time is approximately in inverse proportion to the discharge at theupper stream. The out limit is farther from the upper inflow boundary as discharge increases. Theout limit at the north branch is different from that of the south branch because the discharge intothe north branch is much less than that into the south branch. The data is qualitative similar tothe observed data, which show the three-dimensional advection-diffusion equation can be used toestimate the flushing time and the discharge dominated region of the Yangtze estuary.