OBJECTIVE To evaluate the REGγ expression in gastric cancer tissue and gastric cancer cell lines of various differentiation levels and its clinical significance. METHODS Immunohistochemistry was used to detect the ex...OBJECTIVE To evaluate the REGγ expression in gastric cancer tissue and gastric cancer cell lines of various differentiation levels and its clinical significance. METHODS Immunohistochemistry was used to detect the expression of REGγ protein in 70 specimens of gastric cancer and 30 specimens of normal gastric mucosa. The relationship between the expression of REGγ protein and the biological behaviors of gastric cancer was analyzed. RT-PCR and Western blot were used to detect the mRNA level and the protein expression of REGγ in normal gastric cell line GES-1, well differentiated gastric cancer cell line MKN-28, moderately differentiated gastric cancer cell line SGC-7901 and poorly differentiated gastric cancer cell line BGC-823. RESULTS The expression rate of REGγ protein in gastric cancer tissue （52/70, 74.29%） was significantly higher than that in normal gastric tissue （12/30, 40%） （P 〈 0.01）. The expression rate of REGγ was correlated with tumor size （P 〈 0.01）, lymph node metastasis （P 〈 0.05）, differentiation degree （P 〈 0.01）, infiltration depth （P 〈 0.01） and distant metastasis （P 〈 0.05）. RT-PCR analysis showed that the expression of REGγ mRNA was 0.459 ± 0.079 in the normal gastric mucosa cell ling 0.588 ±0.118 in the well differentiated gastric cancer cell line, 0.715±0.066 in the moderately differentiated gastric cancer cell line, and 0.873 ± 0.099 in the poorly differentiated gastric cancer cell line, showing a negative correlation between REGγ mRNA expression and differentiation level （P 〈 0.05）. Western blot analysis showed that the expression of REGγ protein was 0.712±0.065 in the normal gastric mucosa cell line, 1.176±0.185 in the well differentiated gastric cancer cell line, 1.533 ± 0.127 in the moderately differentiated gastric cancer cell line, and 2.061± 0.398 in the poorly differentiated gastric cancer cell line, showing a negative correlation between REGγ protein expression and differentiation level （P 〈 0.05）. CONCLUSION REGγ is expressed in gastric cancer tissue and normal gastric tissue. In gastric cancer tissues, REGγ expression is positively correlated with the tumor size, lymph node metastasis, differentiation degree, infiltration depth and distant metastasis. Detecting the expression of REGγ mRNA and protein is helpful for early diagnosis and predicting prognosis of gastric cancer.展开更多
On September 29,2013,Shanghai Free Trade Zone("F TZ")was off icially launched upon being ap-proved by the State Council on July 3,2013,covering a geographic area of approximately 28.78 square kilometers,
February 7th,Chinese New Year,will bring about the longest holidayin a year for China's Mainland.Like before,the operation rates at mostchemical firms will hence go down
The Ministry of Industry and InformationTechnology (MⅡT) released a proposed PermitConditions for Manufacturing Pesticidesand expected to implement it all over the countryas soon as it is nodded by the public.The pro...The Ministry of Industry and InformationTechnology (MⅡT) released a proposed PermitConditions for Manufacturing Pesticidesand expected to implement it all over the countryas soon as it is nodded by the public.The proposed permission defines the minimumregistered capital for three categories ofpesticide manufacturers, and the requirementsof location, factory layout, pollutants treatmentfacilities, production installations, qual-展开更多
基金supported by a grant from the National Natural Science Foundation of China(No.30670811).
文摘OBJECTIVE To evaluate the REGγ expression in gastric cancer tissue and gastric cancer cell lines of various differentiation levels and its clinical significance. METHODS Immunohistochemistry was used to detect the expression of REGγ protein in 70 specimens of gastric cancer and 30 specimens of normal gastric mucosa. The relationship between the expression of REGγ protein and the biological behaviors of gastric cancer was analyzed. RT-PCR and Western blot were used to detect the mRNA level and the protein expression of REGγ in normal gastric cell line GES-1, well differentiated gastric cancer cell line MKN-28, moderately differentiated gastric cancer cell line SGC-7901 and poorly differentiated gastric cancer cell line BGC-823. RESULTS The expression rate of REGγ protein in gastric cancer tissue （52/70, 74.29%） was significantly higher than that in normal gastric tissue （12/30, 40%） （P 〈 0.01）. The expression rate of REGγ was correlated with tumor size （P 〈 0.01）, lymph node metastasis （P 〈 0.05）, differentiation degree （P 〈 0.01）, infiltration depth （P 〈 0.01） and distant metastasis （P 〈 0.05）. RT-PCR analysis showed that the expression of REGγ mRNA was 0.459 ± 0.079 in the normal gastric mucosa cell ling 0.588 ±0.118 in the well differentiated gastric cancer cell line, 0.715±0.066 in the moderately differentiated gastric cancer cell line, and 0.873 ± 0.099 in the poorly differentiated gastric cancer cell line, showing a negative correlation between REGγ mRNA expression and differentiation level （P 〈 0.05）. Western blot analysis showed that the expression of REGγ protein was 0.712±0.065 in the normal gastric mucosa cell line, 1.176±0.185 in the well differentiated gastric cancer cell line, 1.533 ± 0.127 in the moderately differentiated gastric cancer cell line, and 2.061± 0.398 in the poorly differentiated gastric cancer cell line, showing a negative correlation between REGγ protein expression and differentiation level （P 〈 0.05）. CONCLUSION REGγ is expressed in gastric cancer tissue and normal gastric tissue. In gastric cancer tissues, REGγ expression is positively correlated with the tumor size, lymph node metastasis, differentiation degree, infiltration depth and distant metastasis. Detecting the expression of REGγ mRNA and protein is helpful for early diagnosis and predicting prognosis of gastric cancer.
文摘On September 29,2013,Shanghai Free Trade Zone("F TZ")was off icially launched upon being ap-proved by the State Council on July 3,2013,covering a geographic area of approximately 28.78 square kilometers,
文摘February 7th,Chinese New Year,will bring about the longest holidayin a year for China's Mainland.Like before,the operation rates at mostchemical firms will hence go down
文摘The Ministry of Industry and InformationTechnology (MⅡT) released a proposed PermitConditions for Manufacturing Pesticidesand expected to implement it all over the countryas soon as it is nodded by the public.The proposed permission defines the minimumregistered capital for three categories ofpesticide manufacturers, and the requirementsof location, factory layout, pollutants treatmentfacilities, production installations, qual-
