A study of toad infestation was carried out from July to December 2022, in the vicinity of the Mfilou and Ngamboulou Rivers, with the aim of determining the prevalence rate of parasitic carriage in these toads. After ...A study of toad infestation was carried out from July to December 2022, in the vicinity of the Mfilou and Ngamboulou Rivers, with the aim of determining the prevalence rate of parasitic carriage in these toads. After collecting 289 specimens, all belonging to the Anuran order and Bufonidae family, three species were identified: Sclerophrys camerunensis (Parker, 1936), Sclerophrys regularis (Reuss, 1834) and Sclerophrys sp. The most abundant species were Sclerophrys camerunensis, followed by Sclerophrys regularis. With regard to parasitological aspects, of the 289 specimens collected, 195 were parasitized, i.e. a percentage of 67.47%. The only parasitic species identified was Ascaris sp., which is preferentially located in the intestine.展开更多
In this paper, we present a regularized Newton method (M-RNM) with correction for minimizing a convex function whose Hessian matrices may be singular. At every iteration, not only a RNM step is computed but also two c...In this paper, we present a regularized Newton method (M-RNM) with correction for minimizing a convex function whose Hessian matrices may be singular. At every iteration, not only a RNM step is computed but also two correction steps are computed. We show that if the objective function is LC<sup>2</sup>, then the method posses globally convergent. Numerical results show that the new algorithm performs very well.展开更多
The present study was carried out to investigate the histological and histochemical changes in the liver and skin on different developmental stages of Egyptian toad Bufo regularis to be used as a histological key for ...The present study was carried out to investigate the histological and histochemical changes in the liver and skin on different developmental stages of Egyptian toad Bufo regularis to be used as a histological key for such species. Our experiment started when tadpoles began to feed. The adapted embryos are divided into 3 large tanks of 200 embryos each, collections of samples started from feeding age every three days. Both histological and histochemical results showed that the general architecture of the different organs was correlated with the state of development, i.e. larval, met-amorphic and post-metamorphic. They, therefore, displayed different characteristic features de-pending on the investigated developmental stage starting from the larval stage (stage 44) and ending with the post-metamorphic stage 66.展开更多
The present study aimed to investigate changes in the protein contents using SDS-PAGE, also using the random amplified polymorphic DNA (RAPD-PCR) assay to identify changes in DNA concentration in the embryos of Egypti...The present study aimed to investigate changes in the protein contents using SDS-PAGE, also using the random amplified polymorphic DNA (RAPD-PCR) assay to identify changes in DNA concentration in the embryos of Egyptian toad Bufo regularis. Our experiment started when tadpoles began to feed. The adapted embryos were divided into 3 large tanks of 200 embryos each, collections of samples started from feeding age every three days. Cluster method was used to indicate the distinct distance between the band patterns of different stages. An increase occurred in the concentration of protein fractions in these tadpoles upon metamorphosis and that when metamorphosis was completed. DNA concentration exhibited an evident low values at the premetamorphic stage 44 (105.85 ± 0.519 ng/μl) compared with the other more advanced developmental stages 55 and 56 (204.056 ± 0.651 ng/μl and 234.55 ± 2.325 ng/μl respectively). In conclusion, acquire information about the changes in the molecular content of Bufo regularis during metamorphosis has been estimated.展开更多
文摘A study of toad infestation was carried out from July to December 2022, in the vicinity of the Mfilou and Ngamboulou Rivers, with the aim of determining the prevalence rate of parasitic carriage in these toads. After collecting 289 specimens, all belonging to the Anuran order and Bufonidae family, three species were identified: Sclerophrys camerunensis (Parker, 1936), Sclerophrys regularis (Reuss, 1834) and Sclerophrys sp. The most abundant species were Sclerophrys camerunensis, followed by Sclerophrys regularis. With regard to parasitological aspects, of the 289 specimens collected, 195 were parasitized, i.e. a percentage of 67.47%. The only parasitic species identified was Ascaris sp., which is preferentially located in the intestine.
文摘In this paper, we present a regularized Newton method (M-RNM) with correction for minimizing a convex function whose Hessian matrices may be singular. At every iteration, not only a RNM step is computed but also two correction steps are computed. We show that if the objective function is LC<sup>2</sup>, then the method posses globally convergent. Numerical results show that the new algorithm performs very well.
文摘The present study was carried out to investigate the histological and histochemical changes in the liver and skin on different developmental stages of Egyptian toad Bufo regularis to be used as a histological key for such species. Our experiment started when tadpoles began to feed. The adapted embryos are divided into 3 large tanks of 200 embryos each, collections of samples started from feeding age every three days. Both histological and histochemical results showed that the general architecture of the different organs was correlated with the state of development, i.e. larval, met-amorphic and post-metamorphic. They, therefore, displayed different characteristic features de-pending on the investigated developmental stage starting from the larval stage (stage 44) and ending with the post-metamorphic stage 66.
文摘The present study aimed to investigate changes in the protein contents using SDS-PAGE, also using the random amplified polymorphic DNA (RAPD-PCR) assay to identify changes in DNA concentration in the embryos of Egyptian toad Bufo regularis. Our experiment started when tadpoles began to feed. The adapted embryos were divided into 3 large tanks of 200 embryos each, collections of samples started from feeding age every three days. Cluster method was used to indicate the distinct distance between the band patterns of different stages. An increase occurred in the concentration of protein fractions in these tadpoles upon metamorphosis and that when metamorphosis was completed. DNA concentration exhibited an evident low values at the premetamorphic stage 44 (105.85 ± 0.519 ng/μl) compared with the other more advanced developmental stages 55 and 56 (204.056 ± 0.651 ng/μl and 234.55 ± 2.325 ng/μl respectively). In conclusion, acquire information about the changes in the molecular content of Bufo regularis during metamorphosis has been estimated.
