In the present study it was proved first that human recombinant interleukin-6(HrIL-6) significantly augmented natural killer(NK) cell activity derived from human fetal spleens against K562 target cells in a 4 hours 51...In the present study it was proved first that human recombinant interleukin-6(HrIL-6) significantly augmented natural killer(NK) cell activity derived from human fetal spleens against K562 target cells in a 4 hours 51Cr release assay. The enhancement of NK activity with 24 hours preincubation in HrlL-6 was dose-dependent, and significantly higher than that of fresh NK cells and controls cultured with RPMI-1640 medium alone (P<0.001). We also found that IL-6 was able to augment NK activity from different fetal spleens at 20 to 40 weeks of gestation (up to 2.24 to 2.78 times), and no difference of NK activity of fetal splenocytes treated by HrIL-6 was observed between different fetal age (32.3% to 45.4%, P>0.05). Furthermore, IL-6-augmented NK activity of fetal splenocytes was very similar to adult levels (P>0.05). These finding strongly indicated that IL-6 plays an important role in the development of NK cell function during the gestational period, suggesting that IL-6 may be of importance in the regulation of host defense mechanisms against malignancies and viral diseases.展开更多
The effects of sesamin on intestinal microflora of mice with normal diet were studied by in vitro animal feces anaerobic culture system.The results showed that treatment with sesamin could significantly change the int...The effects of sesamin on intestinal microflora of mice with normal diet were studied by in vitro animal feces anaerobic culture system.The results showed that treatment with sesamin could significantly change the intestinal flora structure of mice,increasing the abundance of probiotics Lactobacillus and Bifidobacterium and inhibiting the growth of harmful bacteria Enterococcus.High-throughput sequencing analysis showed that sesamin could affect the structure and composition of fecal microflora of mice,and the abundance of probiotics such as Bacillus and Bifidobacterium increased.Sesamin also could increase the expression of tight contiguous protein Occludin and Claudin-1 in colonic tissue of mice,which was benefit to the adhesion of probiotics.In conclusion,sesamin intervention could improve the composition and abundance of intestinal microorganisms in mice with normal diet,which had a beneficial regulatory effect on the intestinal health.展开更多
文摘In the present study it was proved first that human recombinant interleukin-6(HrIL-6) significantly augmented natural killer(NK) cell activity derived from human fetal spleens against K562 target cells in a 4 hours 51Cr release assay. The enhancement of NK activity with 24 hours preincubation in HrlL-6 was dose-dependent, and significantly higher than that of fresh NK cells and controls cultured with RPMI-1640 medium alone (P<0.001). We also found that IL-6 was able to augment NK activity from different fetal spleens at 20 to 40 weeks of gestation (up to 2.24 to 2.78 times), and no difference of NK activity of fetal splenocytes treated by HrIL-6 was observed between different fetal age (32.3% to 45.4%, P>0.05). Furthermore, IL-6-augmented NK activity of fetal splenocytes was very similar to adult levels (P>0.05). These finding strongly indicated that IL-6 plays an important role in the development of NK cell function during the gestational period, suggesting that IL-6 may be of importance in the regulation of host defense mechanisms against malignancies and viral diseases.
基金Support by National Natural Science Foundation of China(Grant No.31872899)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD).
文摘The effects of sesamin on intestinal microflora of mice with normal diet were studied by in vitro animal feces anaerobic culture system.The results showed that treatment with sesamin could significantly change the intestinal flora structure of mice,increasing the abundance of probiotics Lactobacillus and Bifidobacterium and inhibiting the growth of harmful bacteria Enterococcus.High-throughput sequencing analysis showed that sesamin could affect the structure and composition of fecal microflora of mice,and the abundance of probiotics such as Bacillus and Bifidobacterium increased.Sesamin also could increase the expression of tight contiguous protein Occludin and Claudin-1 in colonic tissue of mice,which was benefit to the adhesion of probiotics.In conclusion,sesamin intervention could improve the composition and abundance of intestinal microorganisms in mice with normal diet,which had a beneficial regulatory effect on the intestinal health.
