To observe the effects of different acupuncture manipulations on blood pressure and target organ damage in spontaneously hypertensive rats（SHRs）, this study used the reinforcing twirling method（1.5–2-mm depth; rot...To observe the effects of different acupuncture manipulations on blood pressure and target organ damage in spontaneously hypertensive rats（SHRs）, this study used the reinforcing twirling method（1.5–2-mm depth; rotating needle clockwise for 360° and then counter clockwise for 360°, with the thumb moving heavily forward and gently backward, 60 times per minute for 1 minute, and retaining needle for 9 minutes）, the reducing twirling method（1.5–2-mm depth; rotating needle counter clockwise for 360° and then clockwise for 360°, with the thumb moving heavily backward and gently forward, 60 times per minute for 1 minute, and retaining needle for 9 minutes）, and the needle retaining method（1.5–2-mm depth and retaining the needle for 10 minutes）. Bilateral Taichong（LR3） was treated by acupuncture using different manipulations and manual stimulation. Reinforcing twirling, reducing twirling, and needle retaining resulted in a decreased number of apoptotic cells, reduced Bax m RNA and protein expression, and an increased Bcl-2/Bax ratio in the hippocampus compared with the SHR group. Among these groups, the Bcl-2/Bax protein ratio was highest in the reducing twirling group, and the Bcl-2/Bax m RNA ratio was highest in the needle retaining group. These results suggest that reinforcing twirling, reducing twirling, and needle retaining methods all improve blood pressure and prevent target organ damage by increasing the hippocampal Bcl-2/Bax ratio and inhibiting cell apoptosis in the hippocampus in SHR.展开更多
Objective： To observe the clinical effect of lower-reinforcing and upper-reducing acupuncture method for hyperplasia of mammary gland （HMG） and its influence on estradiol （E2） and progesterone （P）. Methods： A ...Objective： To observe the clinical effect of lower-reinforcing and upper-reducing acupuncture method for hyperplasia of mammary gland （HMG） and its influence on estradiol （E2） and progesterone （P）. Methods： A randomized, single-blinded and controlled trial was conducted. A total of 124 cases conforming to the inclusion criteria were randomized by random number table into a treatment group and a control group, with 62 cases in each group. Patients in both groups received acupuncture therapy at the same acupoints, while patients in the treatment group received lower-reinforcing and upper-reducing method, and patients in the control group received even reinforcing-reducing manipulation. The treatment started around 10 d before menstruation and was conducted every other day. Patients received 5 treatments in each menstruation cycle for consecutive 3 cycles. The levels of E2, P and E2/P and clinical efficacy were measured before and after treatment. Results： After treatment, the breast lump size, pain intensity and concomitant symptoms score in both groups were substantially lower than those before treatment, showing statistical significances （all P〈0.01）, and the improvement in the treatment group was higher than that in the control group, and the between-group comparisons showed statistical significances （all P〈0.01）. After treatment, the overall effective rate was 91.9% in the treatment group, higher than 72.6% in the control group, and the between-group comparison showed a statistical significance （P〈0.01）. After treatment, levels of E2, P and E2/P value showed no statistical significance when compared with those before treatment in the two groups （all P〉0.05）. Conclusion： Lower-reinforcing and upper-reducing acupuncture method can effectively alleviate clinical symptoms and signs in HMG patients, and produce a better effect than even reinforcing-reducing manipulation. The majority of HMG patients＇ E2, P level and E2/P value were not beyond the normal ranges; therefore, acupuncture showed no substantial influence on E2 and P levels and E2/P value.展开更多
Objective To compare the difference in the efficacy on gait time cycle of ischemic stroke between yin-yang respiratory reinforcing and reducing needling technique(yin-yang needling)and the conventional acupuncture.Met...Objective To compare the difference in the efficacy on gait time cycle of ischemic stroke between yin-yang respiratory reinforcing and reducing needling technique(yin-yang needling)and the conventional acupuncture.Methods Sixty cases of ischemic stroke between展开更多
基金supported by the National Natural Science Foundation of China,No.81072861,81373727
文摘To observe the effects of different acupuncture manipulations on blood pressure and target organ damage in spontaneously hypertensive rats（SHRs）, this study used the reinforcing twirling method（1.5–2-mm depth; rotating needle clockwise for 360° and then counter clockwise for 360°, with the thumb moving heavily forward and gently backward, 60 times per minute for 1 minute, and retaining needle for 9 minutes）, the reducing twirling method（1.5–2-mm depth; rotating needle counter clockwise for 360° and then clockwise for 360°, with the thumb moving heavily backward and gently forward, 60 times per minute for 1 minute, and retaining needle for 9 minutes）, and the needle retaining method（1.5–2-mm depth and retaining the needle for 10 minutes）. Bilateral Taichong（LR3） was treated by acupuncture using different manipulations and manual stimulation. Reinforcing twirling, reducing twirling, and needle retaining resulted in a decreased number of apoptotic cells, reduced Bax m RNA and protein expression, and an increased Bcl-2/Bax ratio in the hippocampus compared with the SHR group. Among these groups, the Bcl-2/Bax protein ratio was highest in the reducing twirling group, and the Bcl-2/Bax m RNA ratio was highest in the needle retaining group. These results suggest that reinforcing twirling, reducing twirling, and needle retaining methods all improve blood pressure and prevent target organ damage by increasing the hippocampal Bcl-2/Bax ratio and inhibiting cell apoptosis in the hippocampus in SHR.
文摘Objective： To observe the clinical effect of lower-reinforcing and upper-reducing acupuncture method for hyperplasia of mammary gland （HMG） and its influence on estradiol （E2） and progesterone （P）. Methods： A randomized, single-blinded and controlled trial was conducted. A total of 124 cases conforming to the inclusion criteria were randomized by random number table into a treatment group and a control group, with 62 cases in each group. Patients in both groups received acupuncture therapy at the same acupoints, while patients in the treatment group received lower-reinforcing and upper-reducing method, and patients in the control group received even reinforcing-reducing manipulation. The treatment started around 10 d before menstruation and was conducted every other day. Patients received 5 treatments in each menstruation cycle for consecutive 3 cycles. The levels of E2, P and E2/P and clinical efficacy were measured before and after treatment. Results： After treatment, the breast lump size, pain intensity and concomitant symptoms score in both groups were substantially lower than those before treatment, showing statistical significances （all P〈0.01）, and the improvement in the treatment group was higher than that in the control group, and the between-group comparisons showed statistical significances （all P〈0.01）. After treatment, the overall effective rate was 91.9% in the treatment group, higher than 72.6% in the control group, and the between-group comparison showed a statistical significance （P〈0.01）. After treatment, levels of E2, P and E2/P value showed no statistical significance when compared with those before treatment in the two groups （all P〉0.05）. Conclusion： Lower-reinforcing and upper-reducing acupuncture method can effectively alleviate clinical symptoms and signs in HMG patients, and produce a better effect than even reinforcing-reducing manipulation. The majority of HMG patients＇ E2, P level and E2/P value were not beyond the normal ranges; therefore, acupuncture showed no substantial influence on E2 and P levels and E2/P value.
文摘Objective To compare the difference in the efficacy on gait time cycle of ischemic stroke between yin-yang respiratory reinforcing and reducing needling technique(yin-yang needling)and the conventional acupuncture.Methods Sixty cases of ischemic stroke between
