A hippocampal anti-hypertensive mechanism induced by twirling reinforcing-reducing manipulation in rats

Objective:To investigate a hippocampal anti-hypertensive mechanism induced by twirling reinforcingreducing manipulation(TRRM)using proteomics in rats.Methods:Forty-two male spontaneously hypertensive rats were randomly divided into 3 groups,and 14 Wistar-Kyoto rats were served as control group.In the twirling reinforcing(TRF)group,needles were directly inserted into the Taichong(LR 3)point,then thumbs were moved heavily forward and lightly backward for 3 min,while needles remained inserted for 17 min.In the twirling reduction(TRD)group,the same treatment was applied as in the TRF group except that the thumb moved lightly forward and heavily backward.In the model and control groups,only the corresponding grasping and fixation were applied.All interventions were conducted for 14 days.The blood pressure(BP)of all rats was measured one day before intervention and every other day after.Then hematoxylin-eosin(H&E)staining,label-free and parallel reaction monitoring proteomic techniques were used to assess hippocampal samples from each group.Results:Systolic BPs showed a significant decrease in the TRF and TRD groups compared with the model group(P<.01).In the model group,H&E staining showed obvious pathological changes in the hippocampus,while in the TRF and TRD groups,the hippocampal morphology was only slightly altered.Labelfree proteomic analysis revealed 1163 differential protein expressions between groups.Gene Ontology enrichment analysis confirmed that the differentially expressed proteins were enriched in different biological processes,cellular components,and molecular functions.Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that TRRM proteins were expressed in the serotonergic synapse pathway,renin-angiotensin system,the mitogen-activated protein kinase signaling pathway,and the peroxisome pathway,which were all also related to BP regulation.Conclusion:TRRM can significantly lower the BP of SHRs.The mode of action may be through the activation of various protein pathways in the hippocampus that are related to BP regulation.

Effects of different acupuncture manipulations on protein expression in the parietal cortex of spontaneously hypertensive rats

Objective: To analyze the effects of twirling reinforcing and reducing manipulations on protein expression in parietal cortex of spontaneously hypertensive rats(SHRs), and elucidate the main mechanisms and differences between two manipulations in hypertension treatment.Methods: Rats were randomly divided into the control, model, twirling reinforcing manipulation(TRFM),and twirling reducing manipulation(TRDM) groups. The control and model groups received catch and fixation stimulations once a day for 14 days. The TRFM and TRDM groups were intervened once a day for 20 min for 14 days. On days 0, 2, 6, 10, and 14 after acupuncture, rat systolic blood pressures(SBPs) were measured. Differential protein(DP) expression in the rat parietal cortices was detected. Thereafter, GO functional significance and KEGG pathway enrichment analyses were performed.Results: Compared with the model group, SBP of rats in the TRDM and TRFM groups decreased on days 6 and 10 of acupuncture, respectively(P=.009;P <.001). Moreover, SBP of the TRDM group was significantly lower than that of the TRFM group on days 10 and 14 of acupuncture(P=.015;P=.013).Compared with control group, 601 and 1040 DPs were up-and downregulated, respectively, in the model group. Compared with model group, 44 and 28 up-and downregulated DPs were expressed, respectively,in the TRFM group. Compared with model group, expression of 616 and 427 up-and downregulated DPs,respectively, was found in the TRDM group. After combining the results of GO and KEGG enrichment analyses, five and one pathways were found to be related to the central antihypertensive mechanism of the parietal cortex during twirling reducing and reinforcing manipulations, respectively.Conclusion: TRDM showed a more effective antihypertensive effect on SHRs than TRFM;this antihypertensive effect was related to the regulation of different proteins and their biological functions.

Influence of Sancai and conventional acupuncture manipulations on injured sciatic nerve histomorphology and serum interleukin-6 expression

Acupuncture and moxibustion therapy non-specifically raises the pain threshold, relieves regional muscular tension, improves local blood circulation, accelerates elimination of algogenic substances such as acid metabolites, and promotes neural regeneration and functional rehabilitation. However, the effects of acupuncture are synthetically influenced by multiple factors, which vary with acu-puncture point, intensity, and manipulation. The present study explored the analgesic effects and mechanisms of specific Sancai acupuncture manipulation for sciatica. Results revealed that Sancai acupuncture manipulation significantly reduced inflammatory factor interleukin-6 expression in the blood serum of rats with sciatica, exhibited anti-inflammatory effects and promoted rehabilitation of injured nerves. In addition, Sancai acupuncture manipulation exhibited superior curative effects over conventional acupuncture manipulation.

Clinical Observation of 32 Cases of Scapulohumeral Periarthritis Treated by Manipulations and Acupuncture

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Acupuncture with reinforcing and reducing twirling manipulation inhibits hippocampal neuronal apoptosis in spontaneously hypertensive rats

To observe the effects of different acupuncture manipulations on blood pressure and target organ damage in spontaneously hypertensive rats（SHRs）, this study used the reinforcing twirling method（1.5–2-mm depth; rotating needle clockwise for 360° and then counter clockwise for 360°, with the thumb moving heavily forward and gently backward, 60 times per minute for 1 minute, and retaining needle for 9 minutes）, the reducing twirling method（1.5–2-mm depth; rotating needle counter clockwise for 360° and then clockwise for 360°, with the thumb moving heavily backward and gently forward, 60 times per minute for 1 minute, and retaining needle for 9 minutes）, and the needle retaining method（1.5–2-mm depth and retaining the needle for 10 minutes）. Bilateral Taichong（LR3） was treated by acupuncture using different manipulations and manual stimulation. Reinforcing twirling, reducing twirling, and needle retaining resulted in a decreased number of apoptotic cells, reduced Bax m RNA and protein expression, and an increased Bcl-2/Bax ratio in the hippocampus compared with the SHR group. Among these groups, the Bcl-2/Bax protein ratio was highest in the reducing twirling group, and the Bcl-2/Bax m RNA ratio was highest in the needle retaining group. These results suggest that reinforcing twirling, reducing twirling, and needle retaining methods all improve blood pressure and prevent target organ damage by increasing the hippocampal Bcl-2/Bax ratio and inhibiting cell apoptosis in the hippocampus in SHR.

STUDIES ON REDUCING AND REINFORCING MANIPULATION OF ACUPUNCTURE DURING THE PAST 4 YEARS

Acupuncture manipulation, the pro-cesses from insertion to withdrawal of theneedle, is an important factor in upgradingtherapeutic effect, especially the manipula-tion of reducing and reinforcing when theneedling sensation is obtained. In 1986 LuShoukang et al. made a systemic summaryon acupuncture manipulation in their

Discussion on Reinforcing and Reducing Manipulation of Yin and Yang in Acupuncture

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STUDIES ON REDUCING AND REINFORCING MANIPULATION OF ACUPUNCTURE DURING THE PAST 4 YEARS (Continued)

EXPERIMENTAL STUDIES1. Different effects on physiological in-dices were produced by the different mani-pulations of thrusting--lifting and rotating-twisting. By embedding a probe into theZhusanli point (St 36) of normal male rab-bits for observing the effect of different ma-nipulations, including retention of needle,thrusting--lifting, rapid twisting and slowtwisting on the movements and electricity ofstomach, Liu et al. found that: 1) all

SUBSTITUTION OF ACUPUNCTURE FOR HCG IN OVULATION INDUCTION

By using human menopausal gonadotropin (HMG) and human chorionic gonadotropin (HCG), fairly good clinical therapeutic efficacy has been obtained in the treatment of infertility. However, difficulties are brought about due to the ovarian hyperstimulation syndrome (OHSS) easily induced by these two drugs. Therefore,

EXPERIMENTAL STUDY OF THE ACUPUNCTURE EFFECTS ON CALCIUM ION CONCENTRATION IN RABBITS' ACUPOINTS

In this article, the concentration of calcium ion(Ca21 ) in rabbits’ acupoints hasbeen observed in vivo by means of the Ca21 selective acupuncture electrode (Ca21 SAE). We discov-ered that stimulating the acupoints in the meridian could cause the Ca21 concentration increasing inother points of the same meridian but reducing in the control points （lateral to the meridian points）.The results indicate that Ca21 may involve in the activities of the meridians.

OBSERVATION ON CURATIVE EFFECTS OF BEATING-ACUPUNCTURE ON 100 CASES OF CERVICAL SPONDYLOPATHY

Beating-acupuncture therapy is developed by Li Xutang, Director of the Section of TCM in the Jingdezhen Municipal First Hospital. Amng the 100 cases in the beating-acupuncture group, 85 cases were cured and 11 cases improved, the cure rate was 85 %; Of the 50 cases in the filiform needle acupuncture group, 25 cases were cured and 23 improved, the cure rate was 50 %. There was a statis-tically significant difference(P<0.01 ) between the two grdrips. It is indicated that the curative effect of beating-acupuncture on cervical spondylopathy is obviously superior to that of fili form-needle acupuncture, which provides a new therapy without traumatic pain for clinical treatment of cervical spondylopathy,

THE EFFECT OF WARM ACUPUNCTURE ON RHEUMATOID ARTHRITIS OF 434 CASES

Clinical observation on 434 cases of rheumatoid arthritis shows that the warmingneedle method is markedly effective for rheumatoid arthritis (RA). It also helps to reveal the mecha-nism and function of acupuncture and moxibustion (AM) in curing diseases. Laboratory observationconfirms that the warming needle method can modulate the body’s immunity function significantly. Inthe future, more attention should be paid to point selection and manipulation method so that the bestresult can be obtained.

ACUPUNCTURE TREATMENT OF CORONARY HEART DISEASE

In the present study, the author adopted acupuncture therapy to treat 300 cases ofcoronary heart disease by needling Dahzhong (CV 17 )-to-Jiuwei (CV 15 ), Juque(CV 14 ), Neiguan(PC 6), Zusanli (ST 36), Xinshu (BL 15 ), Jueyinshu (BL 14), etc. and adopting tonification manipulation. Results showed that after treatment, of the 300 cases, 204 (68. 0% ) experienced significant improvement and 84 (28. 0 % ) had improvement, the total effective rate was 96 %. In 201 patients with ischemic ECG, 57 (28. 4% ) had marked amelioration, 93 (46. 2% ) had amelioration, 39(19. 4 % ) had no any apparent change and 12 (6. 0 % ) worsened, with an effective rate of 74. 6 %.Ultrasound cardiogram examination displayed that after acupuncture treatment, the amplitude of vibration of the back wall and the output of the left ventricle increased significantly, the nail fold microcirculation was bettered, and the blood viscosity and the vascular resistance a1l lowered.

124 CASES OF DYSSOMNIA TREATED WITH ACUPUNCTURE AT SISHENCONG POINTS

Dyssomnia is a syndrome consistingmainly in persistent difficulty in fallingasleep,dreamfulness,and early or frequentwakening,leading to dizziness,heaviness inthe head,amnesia and inability of mentalconcentration.The authors treated this ma-lady by acupuncture at Sishencong(Extra 6)with satisfactory effects as reported in thefollowing.

TREATMENT OF HEADACHES BY ACUPUNCTURE AND CHINESE HERBAL THERAPY Conclusive Data Concerning 1000 Patients

IntroductionThis clinical experience concerns 1000patients suffering from headache and treat-ed by acupuncture,moxibustion andChinese herbal therapy during the decade1980-1990.It is a polycentric work.Thetherapy was given in some out-patient de-partments belonging to different local cen-tres of Italian National Health Service:themost rapresentative were the

Observation on the Therapeutic Effect of Acupuncture—Moxibustion in 60 Cases of Diabetes Mellitus

For Yin-deficiency with exuberant-heat type,acupoints of the Yang channelsare selected,viz.,bilateral Geshu,Pishu,Zusanli.A#30 filiform needle is used.Re-ducing method with thrusting-lifting andtwisting-rotating manipulation is appliedand the needle retained for 30 minutes.Fordeficiency of both Qi and Yin type,

Clinical Study on Early Senile Cataract Treated by Acupuncture

40cases/70eyes were treated by need-ling Jingming(UB 1)(without needling ma-nipulation),Fengchi(GB 20),Zusanli(St36),Sanyinjiao(Sp 6)with reinforcing orreducing manipulations by lifting-thrusting,twisting-twirling methods and

VERTIGO TREATED WITH SCALP ACUPUNCTURE

Vertigo is a clinical symptom of variousetiological mechanisms,functional or organ-ic;treatment is therefore different accordingto the type of the disease.Since 1980,the au-thors treated 65 cases of vertigo with goodeffects by scalp acupuncture,the results arereported as follows.

“Tong Guan Li Qiao” Acupuncture Therapy on Post-Stroke Dysphagia

The differences between the“Tong Guan Li Qiao”acupuncture therapy and the conventional and current other acupuncture methods for poststroke dysphagia are as follows:first,it adheres to the basic idea of "Xing Nao Kai Qiao"acupuncture therapy in selecting and combining acupoints,centers on the brain,and combines the dysphagia symptoms of the mouth,tongue,and throat-related orifices with the root cause of"brain";second,thereare strictand standard requirements intheacupuncture operation,that is,manipulation quantification.In addition to standardized twirling,lifting,and thrusting,the techniques of deep needling on the acupoints in the neck region,blood-letting puncturing at the posterior wallof the pharynx,and theneedling sensation of"like a fishbone getting stuck in the throat"are all unique.

OBSERVATION OF CURATIVE EFFECT OF ACUPUNCTURE THERAPY PLUS SCALP ACUPUNCTURE FOR RESTORING CONSCIOUSNESS AND INDUCING RESUSCITATION IN 80 CASES OF ACUTE APOPLEXY

During our 4-year work in The Republic of Yemen, the authors used acupuncture therapy to restore consciousness and induce resuscitation plus scalp acupuncture in 80 cases of acute apoplexy, with 63 cases cured, accounting for 78.75%. This therapy is obviously superior to the traditional principle of point selection in acupuncture used in 25 cases as to curative effect, treatment course and other aspects. There is an obvious statistical difference (P【 0.005).