Replacement of the methylene group at the C-8 position with an extended electronic conjugation is a new promising method to develop red-shifted coelenterazine derivatives. In this paper, we have described an oxygen-co...Replacement of the methylene group at the C-8 position with an extended electronic conjugation is a new promising method to develop red-shifted coelenterazine derivatives. In this paper, we have described an oxygen-containing coelenterazine derivative with a significant red-shifted（63 nm）bioluminescence signal maximum relative to coelenterazine 400a（Deep Blue C^（TM）, 1）. In cell imaging, the sulfur-containing coelenterazine derivative displayed a significantly（1.77 ± 0.09;P≤0.01） higher luminescence signal compared to coelenterazine 400 a and the oxygen-containing coelenterazine derivative exhibited a slightly（0.74 ± 0.08; P≤0.05） lower luminescence signal. It is beneficial to understand further the underlying mechanisms of bioluminescence.展开更多
基金supported by grants from the National Program on Key Basic Research Project (No. 2013CB734000)the National Natural Science Foundation of China (No. 81370085)+1 种基金the Major Project of Science and Technology of Shandong Province (No. 2015ZDJS04001)the Fundamental Research Funds of Shandong University (No. 2014JC008)
文摘Replacement of the methylene group at the C-8 position with an extended electronic conjugation is a new promising method to develop red-shifted coelenterazine derivatives. In this paper, we have described an oxygen-containing coelenterazine derivative with a significant red-shifted（63 nm）bioluminescence signal maximum relative to coelenterazine 400a（Deep Blue C^（TM）, 1）. In cell imaging, the sulfur-containing coelenterazine derivative displayed a significantly（1.77 ± 0.09;P≤0.01） higher luminescence signal compared to coelenterazine 400 a and the oxygen-containing coelenterazine derivative exhibited a slightly（0.74 ± 0.08; P≤0.05） lower luminescence signal. It is beneficial to understand further the underlying mechanisms of bioluminescence.
