Experimental models have allowed inquiry into the pathophysiology of varicocele （VC） beyond that possible with human patients. A randomized controlled study in rats was designed to clarify the influence of the degre...Experimental models have allowed inquiry into the pathophysiology of varicocele （VC） beyond that possible with human patients. A randomized controlled study in rats was designed to clarify the influence of the degree of left renal vein constriction on the development of adolescent VC. Fifty adolescent male Sprague-Dawley rats （Rattus norvegicus） were randomly assigned to five groups of 10： the experimental groups （I-IV） underwent partial ligation of left renal veins with 0.5-, 0.6-, 0.7-, and 0.8-mm diameter needles, respectively. The control group （V） underwent a sham operation. The diameter of the left spermatic vein （LSV） was measured at baseline and 30 days postoperatively. In addition, the lesion of the left kidney was examined with the naked eye and assessed by Masson＇s trichrome staining. VC was successfully induced in 2 （20%）, 4 （40%）, 7 （70%）, and 10 （100%） rats in groups I-IV, respectively. The other rats failed to develop VCs primarily due to left renal atrophy. No VC was observed in group V. The postsurgical LSV diameters in VC rats in groups III and IV were 1.54 ± 0. 16 and 1.49 ± 0.13 mm, respectively （P〉 0.05）, and their increments were 1.36±0. 10 and 1.31±0. 10 ram, respectively （P 〉 0.05）. These results suggest that suitable constriction of the left renal vein is critical for adolescent VC development. In addition, the 0.8-mm diameter needle may be more suitable for inducing left renal vein constriction in adolescent rat models.展开更多
文摘Experimental models have allowed inquiry into the pathophysiology of varicocele （VC） beyond that possible with human patients. A randomized controlled study in rats was designed to clarify the influence of the degree of left renal vein constriction on the development of adolescent VC. Fifty adolescent male Sprague-Dawley rats （Rattus norvegicus） were randomly assigned to five groups of 10： the experimental groups （I-IV） underwent partial ligation of left renal veins with 0.5-, 0.6-, 0.7-, and 0.8-mm diameter needles, respectively. The control group （V） underwent a sham operation. The diameter of the left spermatic vein （LSV） was measured at baseline and 30 days postoperatively. In addition, the lesion of the left kidney was examined with the naked eye and assessed by Masson＇s trichrome staining. VC was successfully induced in 2 （20%）, 4 （40%）, 7 （70%）, and 10 （100%） rats in groups I-IV, respectively. The other rats failed to develop VCs primarily due to left renal atrophy. No VC was observed in group V. The postsurgical LSV diameters in VC rats in groups III and IV were 1.54 ± 0. 16 and 1.49 ± 0.13 mm, respectively （P〉 0.05）, and their increments were 1.36±0. 10 and 1.31±0. 10 ram, respectively （P 〉 0.05）. These results suggest that suitable constriction of the left renal vein is critical for adolescent VC development. In addition, the 0.8-mm diameter needle may be more suitable for inducing left renal vein constriction in adolescent rat models.
