目的探讨富白细胞-血小板纤维蛋白(leukocyte-and platelet-rich fibrin,L-PRF)作为盖髓剂对大鼠牙髓炎症调节的作用。方法脂多糖(LPS)构建牙髓炎大鼠模型,30只大鼠随机分成5组,即正常对照组、LPS造模组、iRoot BP PLUS组、L-PRF组、L-P...目的探讨富白细胞-血小板纤维蛋白(leukocyte-and platelet-rich fibrin,L-PRF)作为盖髓剂对大鼠牙髓炎症调节的作用。方法脂多糖(LPS)构建牙髓炎大鼠模型,30只大鼠随机分成5组,即正常对照组、LPS造模组、iRoot BP PLUS组、L-PRF组、L-PRF+iRoot BP PLUS组。直接盖髓14 d后取标本行显微CT(Micro-CT)扫描、苏木精-伊红(HE)及Masson染色、定量实时聚合酶链式反应(qRT-PCR)检测,观察牙髓炎症及硬组织形成程度。结果Micro-CT示:LPS组未见硬组织形成,L-PRF组冠髓内可见斑块状钙化物,BP组及联合组在穿髓孔处见较完整的钙化桥。组织学染色示:LPS组炎症最重、无硬组织形成;联合组炎症最轻,硬组织形成最完整、连续,两组差异有统计学意义(P<0.01);BP组与联合组的炎症及硬组织形成程度均差异无统计学意义(P>0.05)。qRT-PCR结果示:各实验组牙髓组织中TNF-α、IL-1β、IL-6 mRNA相对表达水平相较于LPS组均有所降低,但L-PRF组与LPS组的差异无统计学意义(P>0.05)。结论L-PRF联合iRoot BP PLUS作为复合盖髓材料,可用于大鼠牙髓炎牙髓组织修复以及牙髓炎症调节。展开更多
The current immunooncology artificially ignores the connection with lymphopoiesis, though is only its derivative. Hematopoietic stem cells (HSC) provide physiological regeneration of biomass of the host, fetus, and ma...The current immunooncology artificially ignores the connection with lymphopoiesis, though is only its derivative. Hematopoietic stem cells (HSC) provide physiological regeneration of biomass of the host, fetus, and malignant tumors, as well, as the cells’ reparation after sub-lethally injuring in any tissues and their renewal. HSC, especially of lymphoid lineage, are the most vulnerable of those, which are responsible for viability of organism. Natural and artificial deficits of HSC determine aging, multi-organs syndromes and death of the host, because their current proliferative resource (CPR) is individually limited at birth, and is spending irreversibly during wounds’ healing, pregnancy, tumor growth, and on. CPR, being an integral value of the number of stem cells along the length of their telomeres, is a “shagreen skin”, for which the tumor competes with normal tissues as a quasi-embryonic favorite and winner, especially in the final period of a shortening the life. The primary approach to cancer treatment must prioritize the preservation of CPR remnants, rather than their destruction, in order to temporarily halt the malignant process. The re-targeting of HSC from tumors in favor of normal tissues is the immediate objective of competitive therapy, which allows for preserving the rest of the CPR host’s resources, especially in patients with advanced cancer. However, the contradictory and insignificant practically, the dogma of antitumor cellular immunity continues to dominate and hinder progress in oncology.展开更多
Coal-fired electricity enterprises are caught in the dilemma of relative fixed prices and rising costs under the scenario of decarbonization.Meanwhile,soaring market-oriented coal pricing results in coal enterprises’...Coal-fired electricity enterprises are caught in the dilemma of relative fixed prices and rising costs under the scenario of decarbonization.Meanwhile,soaring market-oriented coal pricing results in coal enterprises’increasing defaults on thermal coal medium-and long-term contracts(MLC).To investigate the implementation of MLC at the micro-level,this study formalized the contractual behaviors of coal and coal-fired electricity enterprises based on the asymmetric evolutionary game.We formalized the evolving behaviors of both parties using replicator dynamics equations and proved that there were two evolutionary stabilization strategies(ESSs):compliance and coal enterprises’unilateral default.A multi-agent-based simulation was applied to verify the evolving process of ESSs and determine the critical values of MLC design by sensitive analysis.From the simulation results,coal-fired electricity enterprises do not stop generation under the current carbon quota allocation mechanism,even if carbon emission trading increases electricity generation costs.Coal enterprises choose to“default”when the market price of coal is higher than the contracted price by 18%.However,if the original reparation is increased by 5%,the compliance rate of the coal enterprises improves.Dynamic reparations embedded in the MLC improved enforcement during the contracting period.Moreover,the proposed policy implications have practical significance for enhancing the coordinated operation of coal-electricity energy supply chains.展开更多
BACKGROUND Flexible flatfoot(FFF)is a very common condition in children,but no evidencebased guidelines or assessment tools exist.Yet,surgical indication is left to the surgeon’s experience and preferences.AIM To dev...BACKGROUND Flexible flatfoot(FFF)is a very common condition in children,but no evidencebased guidelines or assessment tools exist.Yet,surgical indication is left to the surgeon’s experience and preferences.AIM To develop a functional clinical score for FFF[Catania flatfoot(CTF)score]and a measure of internal consistency;to evaluate inter-observer and intra-observer reliability of the CTF Score;to provide a strong tool for proper FFF surgical indication.METHODS CTF is a medically compiled score of four main domains for a total of twelve items:Patient features,Pain,Clinical Parameters,and Functionality.Each item refers to a specific rate.Five experienced observers answered 10 case reports according to the CTF.To assess inter-and intra-observer reliability of the CTF score,the intra-class correlation coefficients’(ICCs)statistics test was performed,as well as to gauge the correlation between the CTF score and the surgical or conservative treatment indication.Values of 75%were chosen as the score cut-off for surgical indication.Sensitivity,specificity,positive likelihood ratio(PLHR),negative likelihood ratio(NLHR),positive predictive value(PPV),and negative predictive value(NPV).RESULTS Overall interobserver reliability ICC was 0.87[95%confidence interval(CI):0.846-0.892;P<0.001].Overall intra-observer reliability ICC was 0.883(95%CI:0.854-0.909;P<0.001).A direct correlation between the CTF score and surgical treatment indication[Pearson correlation coefficient=0.94(P<0.001)]was found.According to the 75%cut-off,the sensitivity was 100%(95%CI:83.43%-100%),specificity was 85.71%(95%CI:75.29%-92.93%),PLHR was 7(95%CI:3.94-12.43),NLHR was 0(95%CI:0-0),PPV was 75%(95%CI:62.83%-84.19%)and NPV was 100%(95%CI:100%-100%).CONCLUSION CTF represents a useful tool for orthopedic surgeons in the FFF evaluation.The CTF score is a quality questionnaire to reproduce suitable clinical research,survey studies,and clinical practice.Moreover,the 75%cutoff is an important threshold for surgical indication and helps in the decision-making process.展开更多
文摘目的探讨富白细胞-血小板纤维蛋白(leukocyte-and platelet-rich fibrin,L-PRF)作为盖髓剂对大鼠牙髓炎症调节的作用。方法脂多糖(LPS)构建牙髓炎大鼠模型,30只大鼠随机分成5组,即正常对照组、LPS造模组、iRoot BP PLUS组、L-PRF组、L-PRF+iRoot BP PLUS组。直接盖髓14 d后取标本行显微CT(Micro-CT)扫描、苏木精-伊红(HE)及Masson染色、定量实时聚合酶链式反应(qRT-PCR)检测,观察牙髓炎症及硬组织形成程度。结果Micro-CT示:LPS组未见硬组织形成,L-PRF组冠髓内可见斑块状钙化物,BP组及联合组在穿髓孔处见较完整的钙化桥。组织学染色示:LPS组炎症最重、无硬组织形成;联合组炎症最轻,硬组织形成最完整、连续,两组差异有统计学意义(P<0.01);BP组与联合组的炎症及硬组织形成程度均差异无统计学意义(P>0.05)。qRT-PCR结果示:各实验组牙髓组织中TNF-α、IL-1β、IL-6 mRNA相对表达水平相较于LPS组均有所降低,但L-PRF组与LPS组的差异无统计学意义(P>0.05)。结论L-PRF联合iRoot BP PLUS作为复合盖髓材料,可用于大鼠牙髓炎牙髓组织修复以及牙髓炎症调节。
文摘The current immunooncology artificially ignores the connection with lymphopoiesis, though is only its derivative. Hematopoietic stem cells (HSC) provide physiological regeneration of biomass of the host, fetus, and malignant tumors, as well, as the cells’ reparation after sub-lethally injuring in any tissues and their renewal. HSC, especially of lymphoid lineage, are the most vulnerable of those, which are responsible for viability of organism. Natural and artificial deficits of HSC determine aging, multi-organs syndromes and death of the host, because their current proliferative resource (CPR) is individually limited at birth, and is spending irreversibly during wounds’ healing, pregnancy, tumor growth, and on. CPR, being an integral value of the number of stem cells along the length of their telomeres, is a “shagreen skin”, for which the tumor competes with normal tissues as a quasi-embryonic favorite and winner, especially in the final period of a shortening the life. The primary approach to cancer treatment must prioritize the preservation of CPR remnants, rather than their destruction, in order to temporarily halt the malignant process. The re-targeting of HSC from tumors in favor of normal tissues is the immediate objective of competitive therapy, which allows for preserving the rest of the CPR host’s resources, especially in patients with advanced cancer. However, the contradictory and insignificant practically, the dogma of antitumor cellular immunity continues to dominate and hinder progress in oncology.
基金supported by the Fund of Education Ministry Humanity and Society (No. 18YJCZH016)
文摘Coal-fired electricity enterprises are caught in the dilemma of relative fixed prices and rising costs under the scenario of decarbonization.Meanwhile,soaring market-oriented coal pricing results in coal enterprises’increasing defaults on thermal coal medium-and long-term contracts(MLC).To investigate the implementation of MLC at the micro-level,this study formalized the contractual behaviors of coal and coal-fired electricity enterprises based on the asymmetric evolutionary game.We formalized the evolving behaviors of both parties using replicator dynamics equations and proved that there were two evolutionary stabilization strategies(ESSs):compliance and coal enterprises’unilateral default.A multi-agent-based simulation was applied to verify the evolving process of ESSs and determine the critical values of MLC design by sensitive analysis.From the simulation results,coal-fired electricity enterprises do not stop generation under the current carbon quota allocation mechanism,even if carbon emission trading increases electricity generation costs.Coal enterprises choose to“default”when the market price of coal is higher than the contracted price by 18%.However,if the original reparation is increased by 5%,the compliance rate of the coal enterprises improves.Dynamic reparations embedded in the MLC improved enforcement during the contracting period.Moreover,the proposed policy implications have practical significance for enhancing the coordinated operation of coal-electricity energy supply chains.
文摘BACKGROUND Flexible flatfoot(FFF)is a very common condition in children,but no evidencebased guidelines or assessment tools exist.Yet,surgical indication is left to the surgeon’s experience and preferences.AIM To develop a functional clinical score for FFF[Catania flatfoot(CTF)score]and a measure of internal consistency;to evaluate inter-observer and intra-observer reliability of the CTF Score;to provide a strong tool for proper FFF surgical indication.METHODS CTF is a medically compiled score of four main domains for a total of twelve items:Patient features,Pain,Clinical Parameters,and Functionality.Each item refers to a specific rate.Five experienced observers answered 10 case reports according to the CTF.To assess inter-and intra-observer reliability of the CTF score,the intra-class correlation coefficients’(ICCs)statistics test was performed,as well as to gauge the correlation between the CTF score and the surgical or conservative treatment indication.Values of 75%were chosen as the score cut-off for surgical indication.Sensitivity,specificity,positive likelihood ratio(PLHR),negative likelihood ratio(NLHR),positive predictive value(PPV),and negative predictive value(NPV).RESULTS Overall interobserver reliability ICC was 0.87[95%confidence interval(CI):0.846-0.892;P<0.001].Overall intra-observer reliability ICC was 0.883(95%CI:0.854-0.909;P<0.001).A direct correlation between the CTF score and surgical treatment indication[Pearson correlation coefficient=0.94(P<0.001)]was found.According to the 75%cut-off,the sensitivity was 100%(95%CI:83.43%-100%),specificity was 85.71%(95%CI:75.29%-92.93%),PLHR was 7(95%CI:3.94-12.43),NLHR was 0(95%CI:0-0),PPV was 75%(95%CI:62.83%-84.19%)and NPV was 100%(95%CI:100%-100%).CONCLUSION CTF represents a useful tool for orthopedic surgeons in the FFF evaluation.The CTF score is a quality questionnaire to reproduce suitable clinical research,survey studies,and clinical practice.Moreover,the 75%cutoff is an important threshold for surgical indication and helps in the decision-making process.
